用于有机磷农药检测的生物传感器薄膜的研究
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摘要
将纳米金(AuNP)和重氮树脂(DAR)固定在由聚对氨基苯磺酸(PABSA)修饰的玻碳电极表面,构成复合固酶基质膜,用层层自组装技术将乙酰胆碱酯酶(AChE)固定在基质膜上,利用光反应将离子键转化为共价键,形成稳定的生物薄膜.研究固定后的乙酰胆碱酯酶对电流的响应信号,并在此基础上对自组装的工艺进行优化,得到了AChE/DAR/AChE/DAR/AuNP/DAR薄膜,这种生物薄膜是制备生物传感器的理想材料,该方法检测有机磷农药具有很好的稳定性和重现性.
引文
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[2]Zhirong Z,Miao Z,Xiaobin L.Method establishment for the determination of 6 organophosphorous pesticides metabolites in human urine using LC-MS/MS[J].Journal of hygiene research,2013,42(1):122-126.
[3]Guang Z,Zhi-jun Z,Xu-feng D,Yun-guang S,Xi-an G.Detection of estrogenic effects of organophosphorous pesticides on MCF-7 human breast cancer cells[J].Chinese Journal of industrial medicine,2004,17(5):273-276.
[4]Xiang Y,Liu Y,Lee M L.Ultrahigh pressure liquid chromatography using elevated temperature[J].Journal of Chromatography A,2006,6(1-2):198-202.
[5]张秀尧,蔡欣欣,陈珞洛等.缓冲QuEChERS法提取气相色谱火焰光度检测法快速测定水果、蔬菜39种有机磷农药残留[J].中国卫生检验杂志,2007,17(12):2133-2135.
[6]Serge V.D,Alexander A.S,Alexei P.S,Andre'M.N H,Nicole J R,Claude M.Conductometric biosensors based on cholinesterases for sensitive detection of pesticides[J].Electroanalysis,2005,6(9):752-758.
[7]Zourob M,Simonian A,Wild J,Mohr S,Xudong F,Abdulhalim L,Goddard N J.Optical leaky waveguide biosensors for the detection of organophosphorous pesticides[J].The Analyst,2007,132(2):114-120.
[8]Petr S,Gilvanda S N,Hideko Y,Maria L R.Detection of carbamate pesticides in vegetable samples using cholinesterase-based biosensors[J].Electroanalysis,2005,9(14):1083-1087.
[9]Gamal M.Electrochemical Biosensors for the Detection of Pesticides[J].Electrochemistry,2010,2(1):22-42.
[10]Bucur B,Fournier D,Danet A A.Biosensors based onhighly sensitive acetylcholinesterases for enhanced carbamate insecti-cides detection[J].Analytica Chimica Acta,2006,1;115-121.
[11]Miroslav P,Daniel J,Kamil K.Amperometric Biosensors for Real Time Assays of Organophosphates[J].Sensors,2008,8:5303-5312.
[12]Guanglei F,Zhifei D.Efficient immobilization of glucose oxidase by in situ photo-cross-linking for glucose biosensing[J].Talanta,2012,97:438.
[13]Frens G.Controlled nucleation for the regulation of the Particle size in monodisperse gold suspensions[J].Nature Phys Sci,1973,241:20-22.
[14]Yu B,Shuang Z,Kai Z,Changqing S.Covalently attached multilayer assemblies of citrate-cappedcolloidal gold nanoparticles and diazo-resins[J].Colloids and surfaces,2006,218:105-112.
[15]Li X,Wan Y,Sun C.Covalent modification of a glassy carbon surface by electrochemical oxidation of r-aminobenzene sulfonic acid in aqueous solution[J].Electroanal.Chem.,2004,56:79-87.
[16]Dan D,Jiawang D,Jie C,Aidong Z.One-step electrochemically deposited interface of chitosan-gold nanoparticles for acetylcholinesterase biosensor design[J].Journal of Electroanalytical Chemistry,2007,605:53-60.
[17]Jose M P,Paloma Y S,Araceli G C.Gold nanoparticle-based electrochemical biosensors[J].Electrochimica Acta,2008,53:5848-5866.
[18]Bikash K J,C.R R.Electrochemical Biosensor Based on Intergrated Assembly of Dehydrogenase Enzymes and Gold Nanoparticles[J].Anal.Chem,2008,78:6332-6339.
[19]Paulo R.Brasil O M,Anabel L,Susana C.Double-Tagging Polymerase Chain Reaction with a Thiolated Primer and Electrochemical Genosensing based on Gold Nanocomposite Sensor for Food Safety[J].Anal.Chem,2009,81:1332-1339.
[20]Crooks R M,Zhao M,Sun L,Chechik L,Yeung L.Dendrimerencapsulated metal nanoparticles:synthesis,characterization,and applications to catalysis[J].Acc.Chem.Res,2001,34(18):1-19.
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