麦胚多肽-钙螯合物的工艺优化以及结构表征

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• 英文论文题名：Optimization of preparation and structure characterization of calcium-chelating polypeptides from wheat germ
• 论文作者：丁媛媛 ; 王莉 ; 陈正行
• 英文论文作者：Ding Yuanyuan ; Wang Li ; Chen Zhengxing ; School of Food Science and Technology ; National Engineering Laboratory for Cereal Fermentation Technology ; Jiangnan University
• 年：2016
• 作者机构：江南大学食品学院;粮食发酵工艺与技术国家工程实验室;
• 论文关键词：麦胚多肽 ; 螯合钙 ; 结构表征
• 英文论文关键词：wheat germ polypeptides ; chelated calcium ; structure characterization
• 会议召开时间：2016-11-09
• 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
• 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
• 语种：中文
• 分类号：TS201.2
• 学会代码：ZGSP
• 会议名称：中国食品科学技术学会第十三届年会
• 会议地点：中国北京
• 主办单位：中国食品科学技术学会
• 学会名称：中国食品科学技术学会
• 页数：2
• 文件大小：109k
• 原文格式：O
• 会议级别：全国

摘要

为减少小麦胚芽的资源浪费提高其附加值,同时解决居民缺钙的问题,对麦胚多肽与钙离子螯合的工艺进行研究。以小麦胚芽为原料,以螯合率为考察指标,设计单因素试验和正交试验,考察温度、麦胚多肽与钙离子的摩尔比值、时间、pH值对螯合率影响,在温度65℃、麦胚多肽与钙离子的摩尔比值3:1、时间60min、pH9.5的条件下,螯合率达到最大为86.21%。对在最佳条件下制备的麦胚多肽-钙螯合物进行结构分析,红外光谱说明麦胚多肽的氨基和羧基都参与了螯合反应;圆二色谱表明螯合物的二级结构由原来的无序结构向有序、紧密的结构转变;扫描电镜表明螯合物的分子有明显的聚集现象。本研究中麦胚多肽-钙螯合物借助小肽吸收转运机制不仅促进钙离子和氨基酸的利用率,而且为制备新型补钙剂提供理论指导。
In order to achieve value-added utilization of wheat germ and simultaneously reduce resource waste of wheat germ and solve the problem of deficiency of calcium.Wheat germ was used as the raw material to prepare calcium-chelating polypeptides.Under given conditions(temperature,mole ratio between polypeptides and calcium,time and pH),calcium chelating rate was taken as the indexes to optimize the chelating condition.On the basis of the results of single-factor experiments and orthogonal experiment,the optimal chelating conditions were determined as temperature at 65℃ and pH of 9.5 for 60 min with polypeptide to calcium mole ratio of 3:1,yielding maximum calcium chelating rate of 86.21%.Infrared spectroscopic analysis confirmed the coordination reaction between calcium and carboxyl/amino group of polypeptide.Circular dichroism spectra indicated calcium-chelating polypeptides lost its disordered secondary structure and formed compact and ordered conformations compared to the control group.Scanning electron microscopy observation confirmed obvious aggregations of calcium-chelating polypeptides formed.In general,calcium-chelating polypeptides facilitate and enhance the bioavailability of calcium and amino acid with the aid of small peptide absorption,moreover,this study provide a guide for calcium supplement.

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