虫草类真菌的进化生物学研究
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- 论文作者：张永杰 ; 张姝 ; 刘杏忠
- 年：2016
- 作者机构：山西大学生命科学学院;中国科学院微生物研究所;
- 论文关键词：冬虫夏草 ; 蛹虫草 ; 遗传分化 ; 进化 ; 线粒体基因组 ; 普洱茶 ; 微生物组
- 会议召开时间：2016-08-19
- 会议录名称：中国菌物学会2016年学术年会论文摘要集
- 英文会议录名称：Abstracts of 2016 Mycological Society of China Annual Meeting
- 语种：中文
- 分类号：TS272.7;S567.35
- 学会代码：ZGVL
- 会议名称：中国菌物学会2016年学术年会
- 会议地点：中国福建福州
- 主办单位：中国菌物学会
- 学会名称：中国菌物学会
- 页数：2
- 文件大小：1048k
- 原文格式：D
- 会议级别：全国
摘要
作为生物学的一个分支学科,进化生物学(Evolutionary Biology)关注的是生物进化过程和生物多样性。近年来,我们以虫草类真菌为主要研究对象,系统开展了真菌群体遗传与进化、线粒体基因组学、真菌群落及微生物组等方面的研究,得到以下研究结果:1.通过多基因序列分析,发现藏南地区是冬虫夏草菌的起源中心,并进一步发现冬虫夏草菌与寄主昆虫间存在复杂的进化关系。该研究结果对通过划定自然保护区有效地保护冬虫夏草野生资源,以及对指导冬虫夏草人工栽培具有重要的参考价值。同时,该工作对开展其它非模式生物的群体遗传学研究,以及研究其它真菌——宿主体系中双方的互作关系具有借鉴意义。详见参考文献1、7、9、10、12、13、16-20。2.结合经典的分离培养方法和免培养的分子生物学方法,发现天然冬虫夏草群落中具有丰富的、值得开发的真菌资源。该研究结果增加了对冬虫夏草群落中真菌多样性的认识,丰富了我国的药用真菌资源,而且对冬虫夏草的人工规模化培育和深度开发具有重要意义。相关研究见参考文献8、14、15、22和23。3.通过比较线粒体基因组学分析,发现蛹虫草在线粒体DNA上具有明显的遗传分化,不同菌株表现出线粒体内含子的插入缺失多样性,筛选到适于遗传多样性研究的线粒体DNA片段。该工作不但揭示了蛹虫草在线粒体DNA上较高的遗传分化,而且将极大促进蛹虫草群体遗传结构、线粒体遗传机制等研究工作的开展。详见参考文献6、11和21。4.首次报道Hirsutellaminnesotensis、Hirsutellarhossiliensis、Pseudogymnoascuspannorum等真菌的线粒体基因组序列,并且发现利用线粒体全基因组序列构建的系统发育关系与传统的基于细胞核多基因片段构建的系统发育关系基本一致。该工作不但丰富了真菌线粒体基因组数据,而且有助于阐明一些疑难物种的进化和系统发育关系。详见参考文献3-5。5.通过高通量测序发现普洱生茶、普洱熟茶和新鲜茶树叶片具有显著不同的微生物群落结构,贮藏年份是影响普洱茶微生物群落结构的主要因素。通过质谱分析,在普洱茶中检测到多种毒素成分。该工作较好地解释了微生物在普洱茶形成过程中的作用,而且对于指导普洱茶安全饮用具有重要意义。详见参考文献2。
引文
Zhang Y.J.,Hou J.X.,Zhang S.,Hausner G.,Liu X.Z.,Li W.J.The intronicminisatellite Os Min1 within a serine protease gene in the Chinese caterpillar fungus Ophiocordycepssinensis.Applied Microbiology and Biotechnology,2016,100(8):3599-3610.
Zhang Y.J.,Skaar I.,Sulyok M.,Liu X.Z.,Rao M.Y.,Taylor J.W.The microbiome and metabolites in fermented Pu-erh tea as revealed by high-throughput sequencing and quantitative multiplex metabolite analysis.PLo S One,2016,11(6):e0157847.
Zhang,Y.J.,Zhang S.,Liu X.Z.The complete mitochondrial genome of the nematode endoparasitic fungus Hirsutellaminnesotensis.Mitochondrial DNA Part A,2016,27(4):2693-2694.
Zhang,Y.J.,Huo L.Q.,Zhang S.,Liu X.Z.The complete mitochondrial genome of the cold-adapted fungus Pseudogymnoascuspannorum syn.Geomycespannorum.Mitochondrial DNA Part A,2016,27(4):2566-2567.
Wang N.N.,Zhang Y.J.,Hussain M.,Li K.,Xiang M.C.,Liu X.Z.The mitochondrial genome of the nematode endoparasitic fungus Hirsutellarhossiliensis.Mitochondrial DNA Part B,2016,1(1):114-115.
Zhang Y.J.,Zhang S.,Zhang G.Z.,Liu X.Z.,Wang C.S.,Xu J.P.Comparison of mitochondrial genomes provides insights into intron dynamics and evolution in the caterpillar fungus Cordycepsmilitaris.Fungal Genetics and Biology,2015,77:95-107.
Zhang Y.J.,Zhang S.,Li Y.L.,Ma S.L.,Wang C.S.,Xiang M.C.,Liu X.,An Z.Q.,Xu J.P.,Liu X.Z.Phylogeography and evolution of a fungal-insect association on the Tibetan Plateau.Molecular Ecology,2014,23(21):5337-5355.
Zhang S.,Zhang Y.J.,Liu X.Z.,Zhang H.,Liu D.S.On the reliability of DNA sequences of Ophiocordycepssinensis in public databases.Journal of Industrial Microbiology&Biotechnology,2013,40:365-378.
Hu X.,Zhang Y.J.,Xiao G.H.,Zheng P.,Xia Y.L.,Zhang X.Y.,St Leger R.J.,Liu,X.Z.,Wang C.S.Genome survey uncovers the secrets of sex and lifestyle in caterpillar fungus.Chinese Science Bulletin,2013,58(23):2846-2854.
Zhang Y.J.,Bai F.R.,Zhang S.,Liu X.Z.Determining novel molecular markers in the Chinese caterpillar fungus Ophiocordycepssinensis by screening a shotgun genomic library.Applied Microbiology and Biotechnology,2012,95:1243-1251.
Shrestha B.,Zhang W.M.,Zhang Y.J.,Liu X.Z.The medicinal fungus Cordycepsmilitaris:research and development.Mycological Progress,2012,11:599-614.
Zhang Y.J.,Li E.W.,Wang C.S.,Li Y.L.,Liu X.Z.Ophiocordycepssinensis,the flagship fungus of China:terminology,life strategy and ecology.Mycology,2012,3(1):2-10.
Zhang S.,Zhang Y.J.,Liu,X.Z.,Wen H.A.,Wang M.,Liu D.S.Cloning and analysis of the MAT1-2-1 gene from the traditional Chinese medicinal fungus Ophiocordycepssinensis.Fungal Biology,2011,115(8):708-714
Zhang Y.J.,Zhang S.,Wang M.,Bai F.Y.,Liu X.Z.High diversity of the fungal community structure in naturally-occurring Ophiocordycepssinensis.PLo S ONE,2010,5(12):e15570.
Zhang Y.J.,Zhang S.,Liu X.Z.,Wen H.A.,Wang M.A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains.Letters in Applied Microbiology,2010,51(1):114-118.
Shrestha B.,Zhang W.M,Zhang Y.J.,Liu X.Z.What is the Chinese caterpillar fungus Ophiocordycepssinensis(Ophiocordycipitaceae)?Mycology,2010,1(4):228-236
Zhang Y.J.,Xu L.L.,Zhang S.,Liu X.Z.,An Z.Q.,Wang M.,Guo Y.L.Genetic diversity of Ophiocordycepssinensis,a medicinal fungus endemic to the Tibetan Plateau:Implications for its evolution and conservation.BMC Evolutionary Biology,2009,9:290.
Zhang Y.J.,Liu X.Z.,Wang M.Cloning,expression and characterization of two novel cuticle-degrading serine proteases from the entomopathogenic fungus,Cordycepssinensis.Research in Microbiology,2008,159(6):462-469.
张姝,张永杰,SHRESTHA Bhushan,徐建平,王成树,刘杏忠.冬虫夏草菌和蛹虫草菌的研究现状、问题及展望.菌物学报,2013,32(4):577-597.
张姝,张永杰.冬虫夏草菌三个细胞核蛋白编码基因的分子进化.微生物学通报,2015,42(8):1549-1560.
张永杰,郭丽宏,张姝,刘杏忠.适于蛹虫草遗传多样性研究的线粒体分子标记的筛选.微生物学报,2015,55(7):826-833.
张永杰,孙炳达,张姝,旺姆,刘杏忠,巩文峰.分离自冬虫夏草可培养真菌的多样性研究.菌物学报,2010,29(4):518-527.
旺姆,孙漫红,张永杰,罗章,卓嘎.西藏冬虫夏草微生物区系初步研究.中国食用菌,2006,25(suppl.):6-8.
Zhang Y.J.,Skaar I.,Sulyok M.,Liu X.Z.,Rao M.Y.,Taylor J.W.The microbiome and metabolites in fermented Pu-erh tea as revealed by high-throughput sequencing and quantitative multiplex metabolite analysis.PLo S One,2016,11(6):e0157847.
Zhang,Y.J.,Zhang S.,Liu X.Z.The complete mitochondrial genome of the nematode endoparasitic fungus Hirsutellaminnesotensis.Mitochondrial DNA Part A,2016,27(4):2693-2694.
Zhang,Y.J.,Huo L.Q.,Zhang S.,Liu X.Z.The complete mitochondrial genome of the cold-adapted fungus Pseudogymnoascuspannorum syn.Geomycespannorum.Mitochondrial DNA Part A,2016,27(4):2566-2567.
Wang N.N.,Zhang Y.J.,Hussain M.,Li K.,Xiang M.C.,Liu X.Z.The mitochondrial genome of the nematode endoparasitic fungus Hirsutellarhossiliensis.Mitochondrial DNA Part B,2016,1(1):114-115.
Zhang Y.J.,Zhang S.,Zhang G.Z.,Liu X.Z.,Wang C.S.,Xu J.P.Comparison of mitochondrial genomes provides insights into intron dynamics and evolution in the caterpillar fungus Cordycepsmilitaris.Fungal Genetics and Biology,2015,77:95-107.
Zhang Y.J.,Zhang S.,Li Y.L.,Ma S.L.,Wang C.S.,Xiang M.C.,Liu X.,An Z.Q.,Xu J.P.,Liu X.Z.Phylogeography and evolution of a fungal-insect association on the Tibetan Plateau.Molecular Ecology,2014,23(21):5337-5355.
Zhang S.,Zhang Y.J.,Liu X.Z.,Zhang H.,Liu D.S.On the reliability of DNA sequences of Ophiocordycepssinensis in public databases.Journal of Industrial Microbiology&Biotechnology,2013,40:365-378.
Hu X.,Zhang Y.J.,Xiao G.H.,Zheng P.,Xia Y.L.,Zhang X.Y.,St Leger R.J.,Liu,X.Z.,Wang C.S.Genome survey uncovers the secrets of sex and lifestyle in caterpillar fungus.Chinese Science Bulletin,2013,58(23):2846-2854.
Zhang Y.J.,Bai F.R.,Zhang S.,Liu X.Z.Determining novel molecular markers in the Chinese caterpillar fungus Ophiocordycepssinensis by screening a shotgun genomic library.Applied Microbiology and Biotechnology,2012,95:1243-1251.
Shrestha B.,Zhang W.M.,Zhang Y.J.,Liu X.Z.The medicinal fungus Cordycepsmilitaris:research and development.Mycological Progress,2012,11:599-614.
Zhang Y.J.,Li E.W.,Wang C.S.,Li Y.L.,Liu X.Z.Ophiocordycepssinensis,the flagship fungus of China:terminology,life strategy and ecology.Mycology,2012,3(1):2-10.
Zhang S.,Zhang Y.J.,Liu,X.Z.,Wen H.A.,Wang M.,Liu D.S.Cloning and analysis of the MAT1-2-1 gene from the traditional Chinese medicinal fungus Ophiocordycepssinensis.Fungal Biology,2011,115(8):708-714
Zhang Y.J.,Zhang S.,Wang M.,Bai F.Y.,Liu X.Z.High diversity of the fungal community structure in naturally-occurring Ophiocordycepssinensis.PLo S ONE,2010,5(12):e15570.
Zhang Y.J.,Zhang S.,Liu X.Z.,Wen H.A.,Wang M.A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains.Letters in Applied Microbiology,2010,51(1):114-118.
Shrestha B.,Zhang W.M,Zhang Y.J.,Liu X.Z.What is the Chinese caterpillar fungus Ophiocordycepssinensis(Ophiocordycipitaceae)?Mycology,2010,1(4):228-236
Zhang Y.J.,Xu L.L.,Zhang S.,Liu X.Z.,An Z.Q.,Wang M.,Guo Y.L.Genetic diversity of Ophiocordycepssinensis,a medicinal fungus endemic to the Tibetan Plateau:Implications for its evolution and conservation.BMC Evolutionary Biology,2009,9:290.
Zhang Y.J.,Liu X.Z.,Wang M.Cloning,expression and characterization of two novel cuticle-degrading serine proteases from the entomopathogenic fungus,Cordycepssinensis.Research in Microbiology,2008,159(6):462-469.
张姝,张永杰,SHRESTHA Bhushan,徐建平,王成树,刘杏忠.冬虫夏草菌和蛹虫草菌的研究现状、问题及展望.菌物学报,2013,32(4):577-597.
张姝,张永杰.冬虫夏草菌三个细胞核蛋白编码基因的分子进化.微生物学通报,2015,42(8):1549-1560.
张永杰,郭丽宏,张姝,刘杏忠.适于蛹虫草遗传多样性研究的线粒体分子标记的筛选.微生物学报,2015,55(7):826-833.
张永杰,孙炳达,张姝,旺姆,刘杏忠,巩文峰.分离自冬虫夏草可培养真菌的多样性研究.菌物学报,2010,29(4):518-527.
旺姆,孙漫红,张永杰,罗章,卓嘎.西藏冬虫夏草微生物区系初步研究.中国食用菌,2006,25(suppl.):6-8.
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