应用于生态修复技术土壤真菌的筛选与鉴定
详细信息 查看官网全文
- 英文论文题名：Screening and evaluation of soil fungi for ecological restoration technology
- 论文作者：吴雁雯 ; 张金池 ; 王群 ; 王丽 ; 曹容 ; 王凌剑 ; 王鹰翔 ; 孟苗婧
- 英文论文作者：Wu Yanwen ; Zhang Jinchi ; Wang Qun ; Wang Li ; Cao Rong ; Wang Lingjian ; Wang Yingxiang ; Meng Miaojing ; Key Laboratory of Soil and Water Conservation and Ecological Restoration ; College of Forestry ; Nanjing Forestry University
- 年：2015
- 作者机构：南京林业大学林学院水土保持与生态修复重点实验室;
- 论文关键词：生态修复 ; 土壤真菌 ; 系统发育树
- 英文论文关键词：ecological restoration ; soil fungi ; phylogenetic tree
- 会议召开时间：2015-09-01
- 会议录名称：2015海峡两岸水土保持学术研讨会论文集（下）
- 英文会议录名称：Proceedings of Cross-strait Symposium on Soil and Water Conservation 2015
- 语种：中文
- 分类号：S154.3
- 学会代码：OGSJ
- 会议名称：2015海峡两岸水土保持学术研讨会
- 会议地点：中国山西太原
- 主办单位：中国水土保持学会、台湾中华水土保持学会
- 学会名称：中国水土保持学会
- 页数：8
- 文件大小：3044k
- 原文格式：O
- 会议级别：全国
摘要
从南京幕府山采集岩石表层的土壤样品,采用孟加拉红培养基反复筛选真菌。利用真菌通用引物扩增菌株26SrDNA D1/D2区序列,对扩增产物进行测序。测序结果在GenBank中进行同源性搜索,比对其中的26SrDNA D1/D2区序列,利用软件MEGA5构建分子系统发育树。通过序列分析,鉴定出幕府山石灰岩表层土壤中能够培养的真菌隶属于6个属。通过测定真菌作用后培养液的pH和Ca、Mg元素的浓度,得出能够应用于石灰岩山地的生态修复技术的潜在有效菌种为3种青霉菌、1种毛霉菌和1种卵形孢子菌。
Soil sample were obtained from the surface layer of rocks in Mufu Mountain in Nanjing,fungi were repeatedly screening by Rose Bengal medium.Their rDNA D1/D2 sequences were amplified using fungal universal primers D1/D2 to sequence the amplified products.The results of sequencing were searched in Genbank for their homology,and the part of download 26S sequence that possessed some rep resentative species was compared with.And identified Potential effective for three strains penicillium,a rhizomucor and Gongronella using a constructed molecular phylogenetic tree by sequence analysis with software MEGA5.The cultivation fungus in the surface soil of Mufu Mountain limestone belongs to six genera through sequence analysis.The paper tests the culture medium of pH and the concentration of Ca,Mg.As a consequence,there are 3 kinds of valid fungi,which are of blue mold,a mold and a wool oval spore,that could be used in ecological restoration technology.
Soil sample were obtained from the surface layer of rocks in Mufu Mountain in Nanjing,fungi were repeatedly screening by Rose Bengal medium.Their rDNA D1/D2 sequences were amplified using fungal universal primers D1/D2 to sequence the amplified products.The results of sequencing were searched in Genbank for their homology,and the part of download 26S sequence that possessed some rep resentative species was compared with.And identified Potential effective for three strains penicillium,a rhizomucor and Gongronella using a constructed molecular phylogenetic tree by sequence analysis with software MEGA5.The cultivation fungus in the surface soil of Mufu Mountain limestone belongs to six genera through sequence analysis.The paper tests the culture medium of pH and the concentration of Ca,Mg.As a consequence,there are 3 kinds of valid fungi,which are of blue mold,a mold and a wool oval spore,that could be used in ecological restoration technology.
引文
[1]晏闻博,柳丹,彭丹莉,等.重金属矿山生态治理与环境修复技术进展[J].浙江农林大学学报,2015,32(3):467-477.
[2]王丽,张金池,张小庆,等.土壤保水剂含量对喷播基质物理性质及抗冲性能的影响[J].水土保持学报,2010,24(2):79-82.
[3]张涪平.藏中拉屋铜矿区生态恢复研究[D].华中农业大学,2012.
[4]余悦.黄河三角洲原生演替中土壤微生物多样性及其与土壤理化性质关系[D].山东大学,2012.
[5]吴涛,陈骏,连宾.微生物对硅酸盐矿物风化作用研究进展[J].矿物岩石地球化学通报,2007,26(3):263-268、275.
[6]张捷.地表喀斯特沉积中藻类作用的观察研究[J].地理研究,1992,11(2):26-33.
[7]李林.南京幕府山植物区系及其野生观赏植物资源应用研究[D].南京林业大学,2004.
[8]Hillis D M,Moritz C M,Mable B K(eds).Molecular Systematics.Sinauer Sunderland,Massachusetts,USA,1996.
[9]Thompson JD,Gibson TJ,Plewniak F,etal.The CLUSTAL-X windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools.Nucleic Acids Res,1997,25(24):4876-4882.
[10]唐亮,马香,李明霞,等.苹果属S-RNase基因的进化研究[J].中国农业科学,2013,46(13):2717-2729.
[11]Saitou N,Nei M.The Neighbor-Joining method:a new method for reconstructing phylogenetic trees.Mol Biol Evol,1987,4:406-425.
[12]Jongmans A G,Van B N,Lundstrfm U S,et al.Rock-eating fungi[J].Nature,1997,389:682-683.
[13]Cameselle C,Ricart M T,Nunez M J,et al.Iron removal from kaolin Comparison between"insitu"and"two-stage"bioleaching processes[J].Hydrometallurgy,2003,68:97-105.
[14]Puentea M E,Rodriguez J M C,Ching Y L,et al.Image analysis for quantification of bacterial rock weathering[J].Journal of Microbiological Methods,2006,64:275-286.
[15]范丙全.北方石灰性土壤中青霉菌P8活化难溶磷的作用和机理研究:(D)北京:中国农业科学院土壤肥料研究所,2001.
[16]谢先德,张刚生.微生物-矿物相互作用之环境意义的研究[J].岩石矿物学杂志,2001,20(4):382-385.
[2]王丽,张金池,张小庆,等.土壤保水剂含量对喷播基质物理性质及抗冲性能的影响[J].水土保持学报,2010,24(2):79-82.
[3]张涪平.藏中拉屋铜矿区生态恢复研究[D].华中农业大学,2012.
[4]余悦.黄河三角洲原生演替中土壤微生物多样性及其与土壤理化性质关系[D].山东大学,2012.
[5]吴涛,陈骏,连宾.微生物对硅酸盐矿物风化作用研究进展[J].矿物岩石地球化学通报,2007,26(3):263-268、275.
[6]张捷.地表喀斯特沉积中藻类作用的观察研究[J].地理研究,1992,11(2):26-33.
[7]李林.南京幕府山植物区系及其野生观赏植物资源应用研究[D].南京林业大学,2004.
[8]Hillis D M,Moritz C M,Mable B K(eds).Molecular Systematics.Sinauer Sunderland,Massachusetts,USA,1996.
[9]Thompson JD,Gibson TJ,Plewniak F,etal.The CLUSTAL-X windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools.Nucleic Acids Res,1997,25(24):4876-4882.
[10]唐亮,马香,李明霞,等.苹果属S-RNase基因的进化研究[J].中国农业科学,2013,46(13):2717-2729.
[11]Saitou N,Nei M.The Neighbor-Joining method:a new method for reconstructing phylogenetic trees.Mol Biol Evol,1987,4:406-425.
[12]Jongmans A G,Van B N,Lundstrfm U S,et al.Rock-eating fungi[J].Nature,1997,389:682-683.
[13]Cameselle C,Ricart M T,Nunez M J,et al.Iron removal from kaolin Comparison between"insitu"and"two-stage"bioleaching processes[J].Hydrometallurgy,2003,68:97-105.
[14]Puentea M E,Rodriguez J M C,Ching Y L,et al.Image analysis for quantification of bacterial rock weathering[J].Journal of Microbiological Methods,2006,64:275-286.
[15]范丙全.北方石灰性土壤中青霉菌P8活化难溶磷的作用和机理研究:(D)北京:中国农业科学院土壤肥料研究所,2001.
[16]谢先德,张刚生.微生物-矿物相互作用之环境意义的研究[J].岩石矿物学杂志,2001,20(4):382-385.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |