两种鹿尾中总多糖含量测定
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- 英文论文题名：Determination of Polysaccharidesin Cauda Cervi of Two Different Species
- 论文作者：张高慧 ; 王峰 ; 王玉方 ; 李艳 ; 姜英 ; 李淑芬
- 英文论文作者：Zhang Gaohui ; Wang Feng ; Wang Yufang ; Li Yan ; Jiang ying ; Li Shufen ; Institute of Special Wild Economic Animal and Plant Science ; CAAS
- 年：2016
- 作者机构：中国农业科学院特产研究所;
- 论文关键词：鹿尾 ; 多糖 ; 含量测定 ; 苯酚-硫酸法
- 英文论文关键词：Cauda Cervi ; polysaccharide ; content ; determination ; phenol-sulfuric acid method
- 会议召开时间：2016-06-01
- 会议录名称：“十二五” 鹿科学研究进展
- 语种：中文
- 分类号：R284.1
- 学会代码：ZXMY
- 学会名称：中国畜牧业协会
- 页数：7
- 文件大小：267k
- 原文格式：O
摘要
采用水热回流法提取中药材鹿尾中的总多糖,经三氯乙酸除蛋白、活性炭脱色处理,用苯酚一硫酸比色法对鹿尾样品中的总多糖含量进行测定,检测波长为486nm。结果表明梅花鹿尾中总多糖平均含量为4.70mg·g~(-1),马鹿尾中总多糖平均含量为5.86mg·g~(-1),两种鹿尾中总多糖含量差异不显著(P>0.05);该方法操作简便,结果可靠,适用性强,可以作为鹿尾药材的质量控制方法。
Polysaccharides was extracted from Cauda Cervi by water hot reflux extraction,with protein removed by trichloroacetic acid and then decolored by active carbon.The content of the polysaccharides was determined by phenol-sulfuric acid eolorimetry method at 486 nm.The results showed that the polysaccharides content in Cauda Cervi of Cervus Nippon Temminck is4.70 mg·g~(-1) while in Cervus elaphus Linnaeus is 5.86 mg·g~(-1),and there was no distinct differences in Cauda Cervi of two species(P > 0.05);With the chatacter of simplify operation,reliable result and adaptable,the method can be effectively applied in the control of Cauda Cervi quality.
Polysaccharides was extracted from Cauda Cervi by water hot reflux extraction,with protein removed by trichloroacetic acid and then decolored by active carbon.The content of the polysaccharides was determined by phenol-sulfuric acid eolorimetry method at 486 nm.The results showed that the polysaccharides content in Cauda Cervi of Cervus Nippon Temminck is4.70 mg·g~(-1) while in Cervus elaphus Linnaeus is 5.86 mg·g~(-1),and there was no distinct differences in Cauda Cervi of two species(P > 0.05);With the chatacter of simplify operation,reliable result and adaptable,the method can be effectively applied in the control of Cauda Cervi quality.
引文
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[9]Yuhong Liu,Chunhui Liu,Haining Tan,et al.Sulfation of a polysaccharide obtained from Phellinus ribis and potential biological activities of the sulfated derivatives[J].Carbohydrate Polymers,2009,77(2):370-375.
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[14]Chia Chi Wang,Shyh Chung Chang,Bing Huei Chen.Chromatographic determination of polysaccharides in Lycium barbarum Linnaeus[J].Food Chemistry,2009,116(2):595-603.
[15]师勤,马果玉,徐珞珊,等.比色法测定蒺藜中多糖的含量[J].中国药科大学学报,1997,28(5):291-293.
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[17]李知敏,王伯初,周箐,等.植物多糖提取液的几种脱蛋白方法的比较分析[J].重庆大学学报,2004,27(8):57-59.
[18]刘芳,朱学慧,刘玫,等.冬凌草多糖脱蛋白和脱色方法的研究[J].中药材,2008,31(5):751-753.
[19]谭敏,邱细敏,陆艳艳,等.白术多糖脱蛋白脱色工艺[J].中国新药杂志,2010,19(22):2 100-2 102.
[20]朱越雄,孙海一,曹广力.野生糙皮侧耳子实体多糖的脱色素效果比较[J].光谱实验室,2005,22(5):1 070-1 073.
[21]孙颉,何慧,谢笔钧.活性炭脱色对灵芝水提液活性成分的影响[J].化学工业与工程技术,2001,22(1):5-9.
[22]杨娜,杨栋梁,刘佳佳.颗粒活性炭脱色对发酵大蒜水提液中活性成分的影响[J].食品工业科技,2007,28(5):75-77,80.
[2]董万超,赵景辉,潘久茹,等.梅花鹿七种产品的化学成分研究[J].特产研究,1994,(1):36-43.
[3]季宇彬.中药多糖的化学与药理[M].北京:人民卫生出版社,2005.
[4]张阳.鹿茸等四种商品药材质量评价模式研究[D].沈阳:辽宁中医药大学,2008.
[5]谢建华,申明月,刘昕,等.苦瓜中多糖含量测定方法的研究[J].中国食品添加剂,209-213.
[6]绿茶水溶性多糖含量测定方法研究[D].北京:中国农业科学院,2009.
[7]S.R Sudhamani,R.N Tharanathan,M.S Prasad.Isolation and characterization of an extracellular polysaccharide from Pseudomonas caryophylli CFR 1705[J].carbohydrate polymers,2004,56(4):423-427.
[8]Ye Seul Na,Woo Jung Kim,Sung Min Kim,et al.Purification,characterization and immunostimulating activity of water-soluble polysaccharide isolated from Capsosiphon fulvescens[J].International Immunopharmacology,2010,10(3):364-370.
[9]Yuhong Liu,Chunhui Liu,Haining Tan,et al.Sulfation of a polysaccharide obtained from Phellinus ribis and potential biological activities of the sulfated derivatives[J].Carbohydrate Polymers,2009,77(2):370-375.
[10]马丹.红芪多糖的提取分离纯化及组成分析[D].兰州:兰州大学,2008.
[11]Yuhong Liu,Chunhui Liu,Haining Tan,et al.Sulfation of a polysaccharide obtained from Phellinus ribis and potential biological activities of the sulfated derivatives[J].Carbohydrate Polymers,2009,77(2):370-375.
[12]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2010:附录30.
[13]董群,郑丽伊,方积年.改良的苯酚-硫酸法测定多糖和寡糖含量的研究[J].中国药学杂志,1996,31(9):550-553.
[14]Chia Chi Wang,Shyh Chung Chang,Bing Huei Chen.Chromatographic determination of polysaccharides in Lycium barbarum Linnaeus[J].Food Chemistry,2009,116(2):595-603.
[15]师勤,马果玉,徐珞珊,等.比色法测定蒺藜中多糖的含量[J].中国药科大学学报,1997,28(5):291-293.
[16]钟方晓,任海华,李岩.多糖含量测定方法比较[J].时珍国医国药,2007,18(8):1 916-1 917.
[17]李知敏,王伯初,周箐,等.植物多糖提取液的几种脱蛋白方法的比较分析[J].重庆大学学报,2004,27(8):57-59.
[18]刘芳,朱学慧,刘玫,等.冬凌草多糖脱蛋白和脱色方法的研究[J].中药材,2008,31(5):751-753.
[19]谭敏,邱细敏,陆艳艳,等.白术多糖脱蛋白脱色工艺[J].中国新药杂志,2010,19(22):2 100-2 102.
[20]朱越雄,孙海一,曹广力.野生糙皮侧耳子实体多糖的脱色素效果比较[J].光谱实验室,2005,22(5):1 070-1 073.
[21]孙颉,何慧,谢笔钧.活性炭脱色对灵芝水提液活性成分的影响[J].化学工业与工程技术,2001,22(1):5-9.
[22]杨娜,杨栋梁,刘佳佳.颗粒活性炭脱色对发酵大蒜水提液中活性成分的影响[J].食品工业科技,2007,28(5):75-77,80.
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