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不同栽培方式对白肋烟烟碱转化率及TSNA含量的影响
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摘要
为探索不同栽培方式对白肋烟烟碱转化率及烟草特有亚硝胺(TSNA)含量的影响,采取裂区试验设计方法,以不同烟碱转化率类型的品系为主处理,不同种植规格、行向为副处理和再副处理,进行了垂垄向和垄向烟叶的光照强度、生物碱含量、烟碱转化率及4种TSNA含量的比较分析。结果表明,不同转化率的品系是影响烟碱转化率和TSNA含量的主要因素;不同种植规格、行向及不同垄向烟叶的TSNA含量存在显著的差异;品系与行向及种植规格间亦存在显著的互作效应,且HC(高转化)品系受行向和种植规格的影响大于LC(低转化)品系。初步推断,生物碱代谢、TNSA的形成与积累均与光照强度有关。为此认为,在白肋烟生产上首先要选用低转化率品系,确立合理的栽培规格及行向,努力改善烟株的光照条件,是降低烟叶TSNA含量的有效措施。
Tobacco alkaloids and nitrite are the main precursors TSNA formation. Nitrite is formed by the reduction reaction of nitrate in tobacco leaf. Nicotine, nornicotine, anatabine and anabasine of alkaloids reacts with nitrous acid formed NNK, NNN, NAT and NAB. Alkaloids ordinary tobacco to nicotine-based, nornicotine content is very low, but once the tobacco gene mutation, the rapid formation of nicotine to Nornicotine transformation capacity, nornicotine, total NNN and TSNA content and consequent large modest increase.Lot of research results show that numerous cultural factors are different levels of alkaloids, nicotine conversion and formation of TSNAs and accumulation.The purpose of this study was to explore the different cultivation methods on burley tobacco nicotine conversion rate and TSNA content.The experiment was designed as a split plot design with three replicates, to light intensity, alkaloid content, nicotine conversion and four kinds of TSNA content in a comparative analysis.The results showed that, conversion rate differences between strains is a major factor affecting on the TSNA content,planting with different specifications, and perpendicular to the ridge and parallel on the ridge of the tobacco TSNA content exist significant influence; There is also a significant interaction effect between species and row direction and planting specification;HC strains by to and planting specifications than LC strains. Preliminary inference, the formation and accumulation of alkaloid metabolism, TNSA are related to light intensity.To this end, we should choose the low conversion rate of the production of white rib tobacco, and establish reasonable cultivation standards and row, and strive to improve the lighting conditions of the tobacco plant, and it is an effective measure to reduce the content of TSNA in tobacco leaves.
引文
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