利用基因芯片筛选烤烟早花基因初探
详细信息 查看官网全文
- 英文论文题名：A Preliminary Research on microarray-based gene expression files related to premature flowering gene of flue-cured tobacco
- 论文作者：杨静 ; 陈杰 ; 陈建军 ; 邓世媛 ; 邱妙文 ; 赵伟才 ; 王维
- 英文论文作者：YANG Jing ; CHEN Jie ; CHEN Jianjun ; DENG Shiyuan ; QIU Miaowen ; ZHAO Weicai ; WANG Wei ; Qian Dongnan Zhenyuan Tobacco Company ; College of Agriculture ; South China Agricultural University ; Qian Dongnan Tobacco Company ; Nanxiong Institute of Tobacco Science ; Guangdong Provincial Tobacco Company
- 年：2016
- 作者机构：黔东南州烟草公司镇远县分公司;华南农业大学农学院;黔东南州烟草公司;广东省烟草南雄科学研究所;
- 论文关键词：烤烟 ; 早花 ; 抗早花 ; 基因芯片 ; 基因
- 英文论文关键词：flue-cured tobacco ; premature flowering ; premature flowering resistance ; gene chip ; gene
- 会议召开时间：2016-12-01
- 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草农业主题
- 语种：中文
- 分类号：S572
- 学会代码：ZGYG
- 学会名称：中国烟草学会
- 页数：9
- 文件大小：1471k
- 原文格式：D
摘要
为研究与烟草早花有关的基因,本试验利用安捷伦烟草全基因组基因芯片对烤烟品种K326及其抗早花变异系华烟06、K326LF花芽分化过程茎尖端基因表达谱进行分析,筛选出其中与花芽分化可能有主要关系的基因:AGL20、CCR2、ELF4、ERD7、CBF1、COR413-PM1、EMF2、VIP4、COL1、AGL17、COL9、FPA、SWN等,其中COL2、ELF4的表达差异达到了5倍;AGL62、CBF1、SWN、NAP与FPA的差异大于或等于10倍。该试验为进一步研究烟草抗早花机理及培育抗早花新品种提供参考。
To research the gene of premature flowering tobacco, in this study, we used the Agilent Tobacco Oligo Microarray to deduce the expression profiling of flue-cured tobacco K326 and its premature flowering resistance variety Huayan06, K326 LF under the flower bud differentiation process. Through screening, we concluded genes that may have relation with flower bud differentiation were as follows: AGL20、CCR2、ELF4、ERD7、CBF1、COR413-PM1、EMF2、VIP4、COL1、AGL17、COL9、FPA、SWN etc. The expression difference level of COL2 and ELF4 reached 5 times, AGL62、CBF1、SWN、NAP and FPA was greater than or equal to10 times. This study provided a reference for the further study of flue-cured tobacco premature flowering resistance mechanism and premature flowering resistance varieties breeding.
To research the gene of premature flowering tobacco, in this study, we used the Agilent Tobacco Oligo Microarray to deduce the expression profiling of flue-cured tobacco K326 and its premature flowering resistance variety Huayan06, K326 LF under the flower bud differentiation process. Through screening, we concluded genes that may have relation with flower bud differentiation were as follows: AGL20、CCR2、ELF4、ERD7、CBF1、COR413-PM1、EMF2、VIP4、COL1、AGL17、COL9、FPA、SWN etc. The expression difference level of COL2 and ELF4 reached 5 times, AGL62、CBF1、SWN、NAP and FPA was greater than or equal to10 times. This study provided a reference for the further study of flue-cured tobacco premature flowering resistance mechanism and premature flowering resistance varieties breeding.
引文
[1]刘建锋.烟草早花产生的原因及生产对策[J].陕西农业科学,2005,(2):91,130.
[2]金磊,晋艳,周冀衡,等.烟草早花机理及控制的研究进展[J].中国烟草学报,2008,14(1):58-62.
[3]Franklin,KA.Light and temperature signal crosstalk in plant development[J].Current Opinion in Plant Biology,2009,12:63-68.
[4]Horvath D.Common mechanisms regulate flowering and dormancy[J].Plant Science,2009,177(6):523-531.
[5]Matsuda N,Ikeda K,Kurosaka M,et al.Early Flowering phenotype in transgenic pears(Pyrus communis L.)expressing the Ci FT gene[J].J.Japan.Soc.Hort.Sci.2009,78(4):410–416.
[6]Smykal P,Gennen J,De Bodt S,et al.Flowering of strict photoperiodic Nicotiana varieties in non-inductive conditions by transgenic approaches[J].Plant Mol Biol,2007,65(3):233-242.
[7]李元元.低温诱导烟草早花研究与烟草MADS-box基因的同源克隆[J].中国烟草科学,2011,32(3):21-27.
[8]王小彦.低温诱导烟草早花相关因子的分析[D].河南农业大学硕士论文,2009.
[9]李爽,郑唐春,臧丽娜,等.小黑杨Psn AP1基因转化烟草的研究[J].安徽农业科学,2014,42(8):2278-2281,2308.
[10]Wang Yanwen,Wang Wenchao,Jin Shanghui,et al.Over-expression of a putative poplar glycosyltransferase gene,Pt GT1,in tobacco increases lignin content and causes early flowering[J].Journal of Experimental Botany,2012,63(7):2799-2808.
[11]孙颖,谭晓风,罗敏,等.油桐花芽2个不同发育时期转录组分析[J].林业科学,2014,50(5):70-74.
[12]中国烟草基因组计划取得重大突破绒毛状烟草和林烟草全基因组序列图谱完成[N/OL].中国烟草,2011‐12‐13.http://www.tobacco.gov.cn/html/30/3004/3893491_n.html
[13]全球第一套烟草全基因组基因芯片制作完成[N/OL].中国烟草,2013‐01‐15.http://www.tobacco.gov.cn/html/30/3001/4258838_n.html
[14]烟草全基因组覆瓦式(Tiling)基因芯片设计理念实现及差异基因图谱绘制[J].中国烟草学报,2013,(2):F0002-F0002.
[15]马腾飞,林新春.植物SOC1/AGL20基因研究进展[J].浙江农林大学学报,2013,30(6):930-937.
[16]Han P,García-Ponce B,Fonseca-Salazar G,et al.AGAMOUS-LIKE 17,a novel flowering promoter,acts in a FT-independent photoperiod pathway[J].Plant J.2008,55(2):253-65.
[17]Lee D,Polisensky DH,Braam J.Genome-wide identification of touch-and darkness-regulated Arabidopsis genes:a focus on calmodulin-like and XTH genes[J].New Phytologist,2005,165(2):429-444.
[18]Sonmez C,B?urle I,Magusin A,et al.RNA 3'processing functions of Arabidopsis FCA and FPA limit intergenic transcription[J].Proc Natl Acad Sci USA,2011,108(20):8508-8513.
[19]Willmann M R,Poethig R S.The effect of the floral repressor FLC on the timing and progression of vegetative phase change in Arabidopsis[J].Development,2011,138(4):677-685.
[20]Lee H,Suh S S,Park E,et al.The AGAMOUS-LIKE 20 MADS domain protein integrates floral inductive pathways in Arabidopsis[J].Genes Development,2011,14(18):2366-2376.
[21]Seo E,Lee H,Jeon J,et al.Crosstalk between Cold Response and Flowering in Arabidopsis Is Mediated through the Flowering-Time Gene SOC1 and Its Upstream Negative Regulator FLC[J].The Plant Cell,2009,21:3185–3197.
[22]Tao Zhen,Shen Lisha,Liu Chang,et al.Genome-wide identification of SOC1 and SVP targets during the floral transition in Arabidopsis[J].The Plant Journal,2012,70(4):549-561.
[23]An Xinmin,Ye Meixia,Wang Dongmei,et al.Ectopic expression of a poplar APETALA3-like gene in tobacco causes early flowering and fast growth[J].Biotechnology Letters,2011,33(6):1239-1247.
[24]Kelly A J,Bonnlander M B,Meeks-Wagner D R.NFL,the tobacco homolog of FLORICAULA and LEAFY,is transcriptionally expressed in both vegetative and floral meristems[J].Plant Cell,1995,7:225-234.
[25]艾育芳,陈观水,周以飞,等.早熟油菜成花相关基因LFY的克隆与分析[J].西北植物学报,2012,32(10):1965-1970.
[2]金磊,晋艳,周冀衡,等.烟草早花机理及控制的研究进展[J].中国烟草学报,2008,14(1):58-62.
[3]Franklin,KA.Light and temperature signal crosstalk in plant development[J].Current Opinion in Plant Biology,2009,12:63-68.
[4]Horvath D.Common mechanisms regulate flowering and dormancy[J].Plant Science,2009,177(6):523-531.
[5]Matsuda N,Ikeda K,Kurosaka M,et al.Early Flowering phenotype in transgenic pears(Pyrus communis L.)expressing the Ci FT gene[J].J.Japan.Soc.Hort.Sci.2009,78(4):410–416.
[6]Smykal P,Gennen J,De Bodt S,et al.Flowering of strict photoperiodic Nicotiana varieties in non-inductive conditions by transgenic approaches[J].Plant Mol Biol,2007,65(3):233-242.
[7]李元元.低温诱导烟草早花研究与烟草MADS-box基因的同源克隆[J].中国烟草科学,2011,32(3):21-27.
[8]王小彦.低温诱导烟草早花相关因子的分析[D].河南农业大学硕士论文,2009.
[9]李爽,郑唐春,臧丽娜,等.小黑杨Psn AP1基因转化烟草的研究[J].安徽农业科学,2014,42(8):2278-2281,2308.
[10]Wang Yanwen,Wang Wenchao,Jin Shanghui,et al.Over-expression of a putative poplar glycosyltransferase gene,Pt GT1,in tobacco increases lignin content and causes early flowering[J].Journal of Experimental Botany,2012,63(7):2799-2808.
[11]孙颖,谭晓风,罗敏,等.油桐花芽2个不同发育时期转录组分析[J].林业科学,2014,50(5):70-74.
[12]中国烟草基因组计划取得重大突破绒毛状烟草和林烟草全基因组序列图谱完成[N/OL].中国烟草,2011‐12‐13.http://www.tobacco.gov.cn/html/30/3004/3893491_n.html
[13]全球第一套烟草全基因组基因芯片制作完成[N/OL].中国烟草,2013‐01‐15.http://www.tobacco.gov.cn/html/30/3001/4258838_n.html
[14]烟草全基因组覆瓦式(Tiling)基因芯片设计理念实现及差异基因图谱绘制[J].中国烟草学报,2013,(2):F0002-F0002.
[15]马腾飞,林新春.植物SOC1/AGL20基因研究进展[J].浙江农林大学学报,2013,30(6):930-937.
[16]Han P,García-Ponce B,Fonseca-Salazar G,et al.AGAMOUS-LIKE 17,a novel flowering promoter,acts in a FT-independent photoperiod pathway[J].Plant J.2008,55(2):253-65.
[17]Lee D,Polisensky DH,Braam J.Genome-wide identification of touch-and darkness-regulated Arabidopsis genes:a focus on calmodulin-like and XTH genes[J].New Phytologist,2005,165(2):429-444.
[18]Sonmez C,B?urle I,Magusin A,et al.RNA 3'processing functions of Arabidopsis FCA and FPA limit intergenic transcription[J].Proc Natl Acad Sci USA,2011,108(20):8508-8513.
[19]Willmann M R,Poethig R S.The effect of the floral repressor FLC on the timing and progression of vegetative phase change in Arabidopsis[J].Development,2011,138(4):677-685.
[20]Lee H,Suh S S,Park E,et al.The AGAMOUS-LIKE 20 MADS domain protein integrates floral inductive pathways in Arabidopsis[J].Genes Development,2011,14(18):2366-2376.
[21]Seo E,Lee H,Jeon J,et al.Crosstalk between Cold Response and Flowering in Arabidopsis Is Mediated through the Flowering-Time Gene SOC1 and Its Upstream Negative Regulator FLC[J].The Plant Cell,2009,21:3185–3197.
[22]Tao Zhen,Shen Lisha,Liu Chang,et al.Genome-wide identification of SOC1 and SVP targets during the floral transition in Arabidopsis[J].The Plant Journal,2012,70(4):549-561.
[23]An Xinmin,Ye Meixia,Wang Dongmei,et al.Ectopic expression of a poplar APETALA3-like gene in tobacco causes early flowering and fast growth[J].Biotechnology Letters,2011,33(6):1239-1247.
[24]Kelly A J,Bonnlander M B,Meeks-Wagner D R.NFL,the tobacco homolog of FLORICAULA and LEAFY,is transcriptionally expressed in both vegetative and floral meristems[J].Plant Cell,1995,7:225-234.
[25]艾育芳,陈观水,周以飞,等.早熟油菜成花相关基因LFY的克隆与分析[J].西北植物学报,2012,32(10):1965-1970.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |