玉米根系分泌物对烟草黑胫病菌的抑制活性及其抑菌物质分析
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- 英文论文题名：The inhibition activity of maize root exudates against Phytophthora parasitica and the inhibitive compounds analysis
- 论文作者：张立猛 ; 计思贵 ; 焦永鸽 ; 李江舟 ; 代快 ; 崔永和 ; 赵进龙
- 英文论文作者：ZHANG Limeng ; JI Sigui ; JIAO Yongge ; LI Jiangzhou ; DAI kuai ; CUI yonghe ; ZHAO jinlong ; Yunnan Tobacco Company ; Yuxi Branch
- 年：2016
- 作者机构：云南省烟草公司玉溪市公司;
- 论文关键词：玉米 ; 根系分泌物 ; 烟草黑胫病菌 ; 丁布 ; 门布
- 英文论文关键词：maize ; root exudates ; Phytophthora parasitica ; DIMBOA ; MBOA
- 会议召开时间：2016-12-01
- 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草农业主题
- 语种：中文
- 分类号：S435.72
- 学会代码：ZGYG
- 学会名称：中国烟草学会
- 页数：9
- 文件大小：1161k
- 原文格式：D
摘要
本文研究了玉米根系分泌物对烟草黑胫病菌的抑菌活性。结果表明,玉米根系能吸引烟草疫霉游动孢子,其分泌物质能使孢子裂解。进一步研究表明,玉米根系分泌物对烟草寄生疫霉菌游动孢子的释放、休止孢萌发及菌丝生长均具有抑制活性。HPLC-MS分析结果表明,根系分泌物中存在苯并噁嗪类化合物丁布及其降解产物门布。门布对烟草寄生疫霉菌菌丝的生长具有明显的抑菌活性,浓度0.30mg/m L时抑制率达90.94%。本研究结果表明,玉米根系既能吸引游动孢子,又能分泌对烟草黑胫病菌具有抑制活性的苯并噁嗪类化合物。该结果对合理利用玉米与烤烟轮作控制烟草黑胫病的发生具有重要意义。
In this study, the inhibition activity of maize root exudates against Phytophthora parasitica was tested and the DIMBOA and MBOA in root exudates were identified with the method of HPLC-MS. The results indicated that maize root can not only attract zoospores into rhizosphere, but also secret some substances to rupture the spores. The root exudates showed the inhibition activity against the release of zoospores from sporangia, the germination of cycstospore, and the hyphal growth. The DIMBOA and MBOA were identified in root exudates with the method of HPLC-MS. MBOA also showed antifungal activity against the hyphal growth of P. parasitica. The inhibition ratio reached 90.94% when the concentration of MBOA in media was 0.30mg/m L. In summary, maize root can not only attract the zoospores of P. parasitica, but also secret antifungal compounds DIMBOA and MBOA. This data is useful for us to utilize maize to rotate with tobacco to management the disease infected by P. parasitica.
In this study, the inhibition activity of maize root exudates against Phytophthora parasitica was tested and the DIMBOA and MBOA in root exudates were identified with the method of HPLC-MS. The results indicated that maize root can not only attract zoospores into rhizosphere, but also secret some substances to rupture the spores. The root exudates showed the inhibition activity against the release of zoospores from sporangia, the germination of cycstospore, and the hyphal growth. The DIMBOA and MBOA were identified in root exudates with the method of HPLC-MS. MBOA also showed antifungal activity against the hyphal growth of P. parasitica. The inhibition ratio reached 90.94% when the concentration of MBOA in media was 0.30mg/m L. In summary, maize root can not only attract the zoospores of P. parasitica, but also secret antifungal compounds DIMBOA and MBOA. This data is useful for us to utilize maize to rotate with tobacco to management the disease infected by P. parasitica.
引文
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[7]Bjostard L B and Hibbard B E.6-Methoxy-2-benzoxazolinone:a semiochemical for host1ocation by western corn rootworm larvae[J].Jounal of Chemical Ecology,1992,18(7):931-944.
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[10]Fan KW,Vrijmoed LL,Jones EBG.Zoospore chemotaxis of Mangrove thraustochytrids from Hong Kong[J].Mycologia,2002,94(4):569-578.
[11]Tang C S and Young C C.Collection and identification of allelopathic compounds from the undisturbed root system of bigalta limpograss(Hemarthria altissima)[J].Plant Physiology,1982,69(1):155-160.
[12]Ahmad S,Veyrat N,Gordon-Weeks R,et al.Benzoxazinoid Metabolites Regulate Innate Immunity against Aphids and Fungi in Maize[J].Plant Physiology,2011,157(1):317-327.
[13]朱书生,袁善奎,范洁茹,等.六种杀菌剂对黄瓜霜霉病菌不同发育阶段的影响[J].农药学学报,2005,7(2):119-125.
[14]Bais H P,Loyola-Vargas V M,Flores H E,et al.Root-specific metabolism:the biology and biochemistry of underground organs[J].In Vitro Plant,2001,37(6):730-741.
[15]Walker T S,Bais H P,Grotewold E,et al.Root exudation and rhizosphere biology[J].Plant Physiology,2003,132(1):44-51.
[16]Bais H P,Park S W,Weir T L,et al.How plants communicate using the underground information superhighway[J].Trends in Plant Science,2004,9(1):26-32.
[17]Erwin D C and Ribeiro O K.Phytophthora capsici[C].//Er-win D C,Ribeiro O K(ed),Phytophthora Diseases Worldwide.USA:APS Press,1996,262-268.
[18]Deacon J W.Behavioural responses of fungal zoospores[J].Microbiological Sciences,1988,5(8):249-252.
[19]Ho H H and Hichman C J.Factors governing zoospore responses of Phytophthora megasperma var.sojae to plant roots[J].Canadian Journal of Botany,1967,45(11):1983-1994.
[20]Allen R N and Newhook F J.Chemotaxis of zoospores of Phytophthora cinnamomi to ethanol in capillaries of soil pore dimensions[J].Transactions of the British Mycological Society,1973,61(2):287-302
[21]Cameron J N and Carlile M J.Fatty acids,aldehydes and alcohols as attractants for zoospores of Phytophthora palmivora[J].Nature,1978,271(5644):448-449.
[22]van West P,Morris B M,Reid B,et al.Oomycete plant pathogens use electric fields to target roots[J].Molecular Plant-Microbe Interactions,2002,15(8):790-798.
[23]Bais H P,Weir T L,Perry L G,et al.The role of root exudates in rhizosphere interactions with plants and other[J].Annual Review of Plant Biology,2006,57:233-266.
[24]Carlile M J(1983).Motility,taxis and tropism in Phytophthora[C].//Erwin D C,Bartnicki-Garcia S,Tsao P H,Phytophthora:Its Biology,Taxonomy,Ecology and Pathology.St.Paul,Minnesota:American Phytopathological Society,1983,95-107.
[25]Zhang J,Boone L,Kocz R,et al.At the maize/Agrobacterium interface:natural factors limiting host transformation[J].Chemistry&Biology,2000,7(8):611-621.
[26]Woodward M D,Corcuera L J,Helgeson J P,et al.Decomposition of 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one in aqueous solutions[J].Plant Physiology,1978,61:796-802.
[27]Kumar P,Gagliardo R W,Chilton W S.Soil transformation of wheat and corn metabolites MBOA and DIMBOA into aminophenoxazinones[J].Journal of Chemical Ecology,1993,19(11):2453–2461.
[2]韦发才,莫仁敏,杨再豪,等.烤烟主要病虫害发生特点及防治对策[J].广西植保,2007,20(1):35-37.
[3]孙计平,李雪君,吴照辉,等.烟草黑胫病的研究进展[J].湖北农业科学,2011,50(16):3253-3255.
[4]李天福、王彪、王树会.云南烤烟轮作的现状分析与保障措施[J].中国烟草科学,2006,27(2):48-51.
[5]Niemeyer H M.Hydroxamic acids derived from 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one:key defense chemicals of cereals[J].Journal of Agricultural and Food Chemistry,2009,57(5):1677-1696.
[6]Park S,Takano Y,Matsuura H,et al.Antifungal compounds from the root and root exudate of Zea mays[J].Bioscience,Biotechnology and Biochemistry,2004,68(6):1366-1368.
[7]Bjostard L B and Hibbard B E.6-Methoxy-2-benzoxazolinone:a semiochemical for host1ocation by western corn rootworm larvae[J].Jounal of Chemical Ecology,1992,18(7):931-944.
[8]Hibbard B E and Bjoatard L B.Isolation of corn semiochemicals attractive and repellent to western corn rootworm larvae[J].Jounal of Chemical Ecology,1990,16(12):3425-3439.
[9]Friebe A,Klever W,Sikora R,et a1.Allelochemicals in root exudates of maize[C].//Romeo JT,eds.Phytochemical Signals and Plant-Microbe Interactions.New York:Plenum Press,1998,71-93.
[10]Fan KW,Vrijmoed LL,Jones EBG.Zoospore chemotaxis of Mangrove thraustochytrids from Hong Kong[J].Mycologia,2002,94(4):569-578.
[11]Tang C S and Young C C.Collection and identification of allelopathic compounds from the undisturbed root system of bigalta limpograss(Hemarthria altissima)[J].Plant Physiology,1982,69(1):155-160.
[12]Ahmad S,Veyrat N,Gordon-Weeks R,et al.Benzoxazinoid Metabolites Regulate Innate Immunity against Aphids and Fungi in Maize[J].Plant Physiology,2011,157(1):317-327.
[13]朱书生,袁善奎,范洁茹,等.六种杀菌剂对黄瓜霜霉病菌不同发育阶段的影响[J].农药学学报,2005,7(2):119-125.
[14]Bais H P,Loyola-Vargas V M,Flores H E,et al.Root-specific metabolism:the biology and biochemistry of underground organs[J].In Vitro Plant,2001,37(6):730-741.
[15]Walker T S,Bais H P,Grotewold E,et al.Root exudation and rhizosphere biology[J].Plant Physiology,2003,132(1):44-51.
[16]Bais H P,Park S W,Weir T L,et al.How plants communicate using the underground information superhighway[J].Trends in Plant Science,2004,9(1):26-32.
[17]Erwin D C and Ribeiro O K.Phytophthora capsici[C].//Er-win D C,Ribeiro O K(ed),Phytophthora Diseases Worldwide.USA:APS Press,1996,262-268.
[18]Deacon J W.Behavioural responses of fungal zoospores[J].Microbiological Sciences,1988,5(8):249-252.
[19]Ho H H and Hichman C J.Factors governing zoospore responses of Phytophthora megasperma var.sojae to plant roots[J].Canadian Journal of Botany,1967,45(11):1983-1994.
[20]Allen R N and Newhook F J.Chemotaxis of zoospores of Phytophthora cinnamomi to ethanol in capillaries of soil pore dimensions[J].Transactions of the British Mycological Society,1973,61(2):287-302
[21]Cameron J N and Carlile M J.Fatty acids,aldehydes and alcohols as attractants for zoospores of Phytophthora palmivora[J].Nature,1978,271(5644):448-449.
[22]van West P,Morris B M,Reid B,et al.Oomycete plant pathogens use electric fields to target roots[J].Molecular Plant-Microbe Interactions,2002,15(8):790-798.
[23]Bais H P,Weir T L,Perry L G,et al.The role of root exudates in rhizosphere interactions with plants and other[J].Annual Review of Plant Biology,2006,57:233-266.
[24]Carlile M J(1983).Motility,taxis and tropism in Phytophthora[C].//Erwin D C,Bartnicki-Garcia S,Tsao P H,Phytophthora:Its Biology,Taxonomy,Ecology and Pathology.St.Paul,Minnesota:American Phytopathological Society,1983,95-107.
[25]Zhang J,Boone L,Kocz R,et al.At the maize/Agrobacterium interface:natural factors limiting host transformation[J].Chemistry&Biology,2000,7(8):611-621.
[26]Woodward M D,Corcuera L J,Helgeson J P,et al.Decomposition of 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one in aqueous solutions[J].Plant Physiology,1978,61:796-802.
[27]Kumar P,Gagliardo R W,Chilton W S.Soil transformation of wheat and corn metabolites MBOA and DIMBOA into aminophenoxazinones[J].Journal of Chemical Ecology,1993,19(11):2453–2461.
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