大蒜类似根缘细胞生物学特性及其对疫霉菌的抑制活性
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- 英文论文题名：The Biological Characteristics of Garlic Root Border-like Cells and its Antimicrobial activity against Phytophthora capsici
- 论文作者：焦永鸽 ; 张立猛 ; 计思贵 ; 李江舟 ; 代快 ; 崔永和 ; 赵进龙
- 英文论文作者：JI Sigui ; ZHANG Limeng ; JIAO Yongge ; LI Jiangzhou ; DAI kuai ; CUI yonghe ; ZHAO jinlong ; Yunnan Tobacco Company ; Yuxi Branch
- 年：2016
- 作者机构：云南省烟草公司玉溪市公司;
- 论文关键词：大蒜 ; 辣椒疫霉菌 ; 水培液
- 英文论文关键词：garlic ; P.capsici ; hydroponic liquid
- 会议召开时间：2016-12-01
- 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草农业主题
- 语种：中文
- 分类号：S633.4;S436.418
- 学会代码：ZGYG
- 学会名称：中国烟草学会
- 页数：11
- 文件大小：4436k
- 原文格式：D
摘要
本文分析了大蒜根冠细胞脱落物的种类以及不同温湿度和根长对大蒜根冠细胞脱落物产生的影响,并测定细胞脱落物水培液对辣椒疫霉菌的抑制活性。结果表明,大蒜根冠细胞脱落物为类似根缘细胞,有椭圆/长椭圆形和长形细胞。大蒜类似根缘细胞的产生受根长、温度和湿度的影响。大蒜根系在100ml 2%水琼脂的平板内,20℃条件下培养至25mm以上产生的类似根缘细胞最多。大蒜类似根缘细胞水培液对辣椒疫霉菌游动孢子游动和孢子囊释放都具有显著的抑制活性。该研究表明,大蒜根系产生的类似根缘细胞也是化感抑菌物质释放的重要途径。
This study analyzed the type of garlic sloughed root cap cells and the effect of temperature, humidity and the root length on the production of this kind of cell. The antimicrobial activity of the hydroponic liquid of sloughed root cap cells against Phytophthora capsici leonian was also been tested. The results indicated that the sloughed root cap cells of garlic was border-like cells(BLCs), which includes oval/long oval cells and elongated cells. The production of BLCs was affected by temperature, humidity and the length of roots. Garlic roots can produce a large number of BLCs when garlic roots grow to 25 mm length at 20℃ in plates containing 100 ml 2% water agar. Hydroponic liquid of BLCs showed inhibitory activity against zoospore release from sporangia and zoospores' motility. This study demonstrated that border-like cells is the important way of garlic to release antimicrobial substances.
This study analyzed the type of garlic sloughed root cap cells and the effect of temperature, humidity and the root length on the production of this kind of cell. The antimicrobial activity of the hydroponic liquid of sloughed root cap cells against Phytophthora capsici leonian was also been tested. The results indicated that the sloughed root cap cells of garlic was border-like cells(BLCs), which includes oval/long oval cells and elongated cells. The production of BLCs was affected by temperature, humidity and the length of roots. Garlic roots can produce a large number of BLCs when garlic roots grow to 25 mm length at 20℃ in plates containing 100 ml 2% water agar. Hydroponic liquid of BLCs showed inhibitory activity against zoospore release from sporangia and zoospores' motility. This study demonstrated that border-like cells is the important way of garlic to release antimicrobial substances.
引文
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[14]Kuzyakov Y.Review:Factors affecting rhizosphere priming effects[J].Journal of Plant Nutrition and Soil Science,2002,165(4):382‐396.
[15]张伟,廖静静,朱贵李,等.8种植物挥发物和浸提液对三七根腐病菌的抑制活性研究[J].中国农学通报,2013,29(30):197‐201.
[16]廖静静,刘屹湘,杨敏,等.大蒜挥发物和浸提液对辣椒疫霉菌的抑菌活性分析[J].云南农业大学学报,2014,29(3):337‐346.
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[18]Curlango‐Rivera G,Huskey D A,Mostafa A,et al.Intraspecies variation in cotton border cell production:Rhizosphere microbiome implications[J].American Journal of Botany,2013,100(9):1706‐1712.
[19]Bengough A G,Mc Kenzie B M.Sloughing of root cap cells decrease the frictional resistance to maize(Zea mays L.)root growth[J].Journal of Experimental Botany,1997,48(4):885‐893.
[20]Tamás L,BudíkováS,HuttováJ,et al.Aluminum‐induced cell death of barley‐root border cells is correlated with peroxidase‐and oxalate oxidase‐mediated hydrogen peroxide production[J].Plant Cell Reports,2005,24(3):189‐194.
[21]Miyasaka S C,Hawes M C.Possible role of root border cells in detection and avoidance of aluminum toxicity[J].Plant Physiology,2001,125(4):1978‐1987.
[22]Pan J W,Ye D,Wang L L,et al.Root border cell development is a temperature‐insensitive and Al‐sensitive process in barley[J].Plant Cell Physiology,2004,45(6):751–760.
[23]Brigham L A,Woo H H,Nicoll S M,et al.Differential expression of proteins and m RNAs from border cells and root tips of pea[J].Plant Physiology,1995,109(2):457‐463.
[24]Wen F S,Van Etten H D,Tsaprailis G,et al.Extracellular proteins in pea root tip and border cell exudates[J].Plant Physiology,2007,143(2):773–783.
[25]Cannesan M A,Gangneux C,Lanoue A,et al.Association between border cell responses and localized root infection by pathogenic Aphanomyces euteiches[J].Annals of Botany,2011,108(3):459‐469.
[26]Hawes M C,Lin H J.Correlation of pectolytic enzyme activity with the programmed release of cells from root caps of pea(Pisum sativum)[J].Plant Physiology,1990,94(4):1855‐1859.
[27]Driouich A,Durand C,Cannesan M A,et al.Border cells versus border‐like cells:are they alike?[J].Journal of Experimental Bortany,2010,61(14):3827‐3831.
[28]Hawes M C,Bengough G,Cassab G,et al.Root caps and rhizosphere[J].Plant Growth Regulation,2002,21(4):352‐367.
[29]VicréM,Santaella C,Blanchet S,et al.Root border‐like cells of Arabidopsis.microscopical characterization and role in the interaction with rhizobacteria[J].Plant Physiology,2005,138(2):998‐1008.
[30]Driouich A,Cannesan M A,Dardelle F,et al.Unity is strength:The power of border cells and border‐like cells in relation with plant defense[C]//Vivanco J M,Balu?ka F,eds,Secretions and Exudates in Biological System,Signaling and Communication in Plants.Springer‐Verlag Berlin Heidelperg,2012:91‐107.
[31]Odell R E,Dumlao M R,Samar D,et al.Stage‐dependent border cell and carbon flow from roots to rhizosphere[J].American Journal of Botany,2008,95(4):441‐446.
[2]汤东生,王斌,毛忠顺,等.大蒜根系分泌物对石榴枯萎病菌和枯草芽孢杆菌作用初探[J].农业科学与技术(英文版),2011,12(2):237‐240.
[3]孙雁,周大富,王云月,等.辣椒玉米间作对病害的控制作用及其增产效应[J].园艺学报,2006,33(5):995‐1000.
[4]金杨秀,谢关林,孙祥良,等.大蒜轮作与瓜类枯萎病发病的关系[J].上海交通大学学报,2003,3(1):9‐12.
[5]甄洁,张桂玲,朱信涵,等.西瓜无公害生产的病虫害防治技术[J].农技服务,2007,24(7):54‐55.
[6]向青松,钟亚霖,彭军,等.农业生物多样性控制烟草病虫害[J].中国农学通报,2010,26(2):208‐211.
[7]张学文,刘亦学,刘万学,等.植物化感物质及其释放途径[J].中国农学通报,2007,23(7):295‐297.
[8]Ren L X,Su S M,Yang X M,et al.Intercropping with aerobic rice suppressed Fusarium wilt in watermelon[J].Soil Biology and Biochemistry,2008,40(3):834‐844.
[9]Zewde T,Fininsa C,Sakhuja P K,et al.Association of white rot(Sclerotium cepicorum)of garlic with environmental factors and cultural practices in the North Shewa highlands of Ethiopia[J].Crop Protection,2007,26(10):1566‐1573.
[10]Abdel‐Monaim M F,Abo‐Elyousr K A M.Effect of preceding and intercropping crops on suppression of lentil damping‐off and root rot disease in New Valley,Egypt[J].Crop Protection,2012,32:41–46.
[11]Park S,Takano Y,Matsuura H,et al.Antifungal compounds from the root and root exudates of Zea mays[J].Bioscience Biotechnology and Biochemistry,2004,68(6):1366‐1368.
[12]Michel V V,Wang J F,Midmore D J,et al.Effects of intercropping and soil amendment with urea and calcium oxide on the incidence of bacterial wilt of tomato and survival of soil‐borne Pseudomonas solanacearum in Taiwan[J].Plant Pathology.1997,46(4):600–610.
[13]Dong L,Li X,Huang L,et al.Lauric acid in crown daisy root exudates potently regulates root-knot nematode chemotaxis and distrupts Mi-flp-18 expression to block infection[J].Journal of Experimental Botany,2013,65:131-141.
[14]Kuzyakov Y.Review:Factors affecting rhizosphere priming effects[J].Journal of Plant Nutrition and Soil Science,2002,165(4):382‐396.
[15]张伟,廖静静,朱贵李,等.8种植物挥发物和浸提液对三七根腐病菌的抑制活性研究[J].中国农学通报,2013,29(30):197‐201.
[16]廖静静,刘屹湘,杨敏,等.大蒜挥发物和浸提液对辣椒疫霉菌的抑菌活性分析[J].云南农业大学学报,2014,29(3):337‐346.
[17]张万萍,赵丽.大蒜提取物和根系分泌物对3种土传性病原菌的抑菌效果[J].中国蔬菜,2012(2):66‐71.
[18]Curlango‐Rivera G,Huskey D A,Mostafa A,et al.Intraspecies variation in cotton border cell production:Rhizosphere microbiome implications[J].American Journal of Botany,2013,100(9):1706‐1712.
[19]Bengough A G,Mc Kenzie B M.Sloughing of root cap cells decrease the frictional resistance to maize(Zea mays L.)root growth[J].Journal of Experimental Botany,1997,48(4):885‐893.
[20]Tamás L,BudíkováS,HuttováJ,et al.Aluminum‐induced cell death of barley‐root border cells is correlated with peroxidase‐and oxalate oxidase‐mediated hydrogen peroxide production[J].Plant Cell Reports,2005,24(3):189‐194.
[21]Miyasaka S C,Hawes M C.Possible role of root border cells in detection and avoidance of aluminum toxicity[J].Plant Physiology,2001,125(4):1978‐1987.
[22]Pan J W,Ye D,Wang L L,et al.Root border cell development is a temperature‐insensitive and Al‐sensitive process in barley[J].Plant Cell Physiology,2004,45(6):751–760.
[23]Brigham L A,Woo H H,Nicoll S M,et al.Differential expression of proteins and m RNAs from border cells and root tips of pea[J].Plant Physiology,1995,109(2):457‐463.
[24]Wen F S,Van Etten H D,Tsaprailis G,et al.Extracellular proteins in pea root tip and border cell exudates[J].Plant Physiology,2007,143(2):773–783.
[25]Cannesan M A,Gangneux C,Lanoue A,et al.Association between border cell responses and localized root infection by pathogenic Aphanomyces euteiches[J].Annals of Botany,2011,108(3):459‐469.
[26]Hawes M C,Lin H J.Correlation of pectolytic enzyme activity with the programmed release of cells from root caps of pea(Pisum sativum)[J].Plant Physiology,1990,94(4):1855‐1859.
[27]Driouich A,Durand C,Cannesan M A,et al.Border cells versus border‐like cells:are they alike?[J].Journal of Experimental Bortany,2010,61(14):3827‐3831.
[28]Hawes M C,Bengough G,Cassab G,et al.Root caps and rhizosphere[J].Plant Growth Regulation,2002,21(4):352‐367.
[29]VicréM,Santaella C,Blanchet S,et al.Root border‐like cells of Arabidopsis.microscopical characterization and role in the interaction with rhizobacteria[J].Plant Physiology,2005,138(2):998‐1008.
[30]Driouich A,Cannesan M A,Dardelle F,et al.Unity is strength:The power of border cells and border‐like cells in relation with plant defense[C]//Vivanco J M,Balu?ka F,eds,Secretions and Exudates in Biological System,Signaling and Communication in Plants.Springer‐Verlag Berlin Heidelperg,2012:91‐107.
[31]Odell R E,Dumlao M R,Samar D,et al.Stage‐dependent border cell and carbon flow from roots to rhizosphere[J].American Journal of Botany,2008,95(4):441‐446.
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