劣味乳菇液体发酵工艺及其防治杨树溃疡病研究
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- 论文作者：张扬 ; 叶建仁 ; 张林平 ; 栾丰刚
- 年：2016
- 作者机构：南京林业大学林学院江苏省有害生物入侵预防与控制重点实验室;江西农业大学林学院;
- 论文关键词：劣味乳菇 ; 发酵工艺 ; 溃疡病 ; 防效
- 会议召开时间：2016-08-19
- 会议录名称：中国菌物学会2016年学术年会论文摘要集
- 英文会议录名称：Abstracts of 2016 Mycological Society of China Annual Meeting
- 语种：中文
- 分类号：TQ920.6;S763.7
- 学会代码：ZGVL
- 会议名称：中国菌物学会2016年学术年会
- 会议地点：中国福建福州
- 主办单位：中国菌物学会
- 学会名称：中国菌物学会
- 页数：3
- 文件大小：583k
- 原文格式：D
- 会议级别：全国
摘要
外生菌根是真菌与植物根系相互作用形成的互惠共生体,是大多数森林植物正常生长不可或缺的组成部分,具有重要的生态环境效益和经济价值,外生菌根真菌与植物根系形成菌根共生体可促进根系对土壤养分的吸收提高植物抗逆性、促进植物生长等生态功能,对植物群落多样性及生产力的维持起着重要的作用,为更好地为外生菌根真菌应用提供参考依据和理论基础,以外生菌根真菌劣味乳菇(Lactarius insulsus)为试验材料,采用液体发酵法,分析接种量、溶解氧及p H值等因子对其发酵培养的影响,并将发酵产物制成液体菌剂,对杨树溃疡病进行田间防治试验,田间试验地选在江苏淮安市集沭河旁,树苗来自杨树溃疡病高发区苗圃一年生NL-895杨,采用随机区组设计试验,布置保护行,采用灌根法,株行距4m×8 m,菌剂由发酵液:水=1.5:8.5组成,菌液浓度为15%,菌剂灌根后覆土,每棵树用量500 m L。各处理200棵,并日常维护。接种30 d后调查一年生杨树苗溃疡病发病级别,统计杨树死亡率、病情指数及防病效果,结果表明,接种量5%,发酵过程中p H保持5左右,发酵中后期保持溶氧浓度在28%~35%可有效提高劣味乳菇菌丝体生物量,缩短了发酵周期;田间防治试验表明,劣味乳菇对溃疡病有较好防治效果,杨树苗死亡率可减少19.3%,防效可达41.1%。
引文
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[15]杨蕾,周国英,梁军,2014.防治杨树溃疡病内生菌的分离筛选及鉴定[J].植物保护学报,(4):438-446
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[18]陈辉,唐明,刘贤德,1996.外生菌根真菌对杨树抗溃疡病的影响[J].植物病理学报,26(4):370-370
[19]马磊,吴小芹,2007.九种外生菌根菌与杨树苗木的菌根化研究[J].南京林业大学学报(自然科学版),31(6):29-33
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[22]Shen Q,Chen W,Yan Z,Xie Z,2009.Potential pharmaceutical resources of the Qinling Mountain in central China:medicinal fungi[J].Frontiers of Biology in China,4(1):89-93
[23]Kibar B,Peksen A,2011.Mycelial growth requirements of Lactarius pyrogalus and Lactarius controversus[J].Afr.J.Microbiol.Res,5(28):5107-5114
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[26]任嘉红,班虎栋,叶建仁,2010.吡咯伯克霍尔德氏菌JK-SH007的发酵条件及其对杨树溃疡病的防治效果[J].中国生物防治,26(3):300-306
[27]Ren J H,Ye J R,Liu H,Xu X L,Wu X Q,2011.Isolation and characterization of a new Burkholderia pyrrocinia strain JK-SH007 as a potential biocontrol agent[J].World Journal of Microbiology and Biotechnology,27(9):2203-2215
[2]Adeleke R A,Cloete T E,Bertrand A,Khasa D P,2010.Mobilisation of potassium and phosphorus from iron ore by ectomycorrhizal fungi[J].World Journal of Microbiology and Biotechnology,26(10):1901-1913
[3]Zhang R Q,Tang M,Chen H,Tian Z Q,2011.Effects of ectomycorrhizal fungi on damping-off and induction of pathogenesis-related proteins in Pinus tabulaeformis seedlings inoculated with Amanita vaginata[J].Forest Pathology,41(4):262-269
[4]St.Martin P,Mallik A,2016.Growth release of stunted black spruce(Picea mariana)in Kalmia heath:The role of ectomycorrhizal fungi and near-ground microclimate[J].Canadian Journal of Forest Research,46(5):666-673
[5]Klein T,Siegwolf RT,K?rner C,2016.Belowground carbon trade among tall trees in a temperate forest.Science,352(6283):342-344
[6]Griffiths R,Baham J,Caldwell B,1994.Soil solution chemistry of ectomycorrhizal mats in forest soil[J].Soil Biology and Biochemistry,26(3):331-337
[7]刘辉,吴小芹,陈丹,2010.4种外生菌根真菌对难溶性磷酸盐的溶解能力[J].西北植物学报,30(1):143-149
[8]曾丽琼,2010.几种优良外生菌根菌的应用和胶丸菌剂的制备[D],南京林业大学
[9]张星耀,骆有庆,2004.中国森林重大生物灾害[M],中国林业出版社
[10]伦世仪,2008.生化工程[M],中国轻工业出版社
[11]方中达,1998.植病研究方法[M],农业出版社,1979:6-11
[12]刘会香,贾秀贞,吕全,等,2005.中国杨树溃疡病的发生与防治[J].世界林业研究,18(4):60-63
[13]任飞娟,2011.欧美杨溃疡病病原鉴定[D].北京:北京林业大学
[14]杨蕾,梁军,周国英,2015.土壤中杨树溃疡病生防菌的分离鉴定[J].林业科学,51(4):116-125
[15]杨蕾,周国英,梁军,2014.防治杨树溃疡病内生菌的分离筛选及鉴定[J].植物保护学报,(4):438-446
[16]Colpaert J V,Wevers J H,Krznaric E,Adriaensen K,2011.How metal-tolerant ecotypes of ectomycorrhizal fungi protect plants from heavy metal pollution[J].Annals of Forest Science,68(1):17-24
[17]Luo Z B,Li K,Gai Y,G?bel C,Wildhagen H,Jiang X,Feu?ner I,Rennenberg H,Polle A.,2011.The ectomycorrhizal fungus(Paxillus involutus)modulates leaf physiology of poplar towards improved salt tolerance[J].Environmental and Experimental Botany,72(2):304-311
[18]陈辉,唐明,刘贤德,1996.外生菌根真菌对杨树抗溃疡病的影响[J].植物病理学报,26(4):370-370
[19]马磊,吴小芹,2007.九种外生菌根菌与杨树苗木的菌根化研究[J].南京林业大学学报(自然科学版),31(6):29-33
[20]Zhao L,Wu X Q,Ye J R,Li H,Li G E,2014.Isolation and characterization of a mycorrhiza helper bacterium from rhizosphere soils of poplar stands[J].Biology and Fertility of Soils,50(4):593-601
[21]Peter M,Büchler U,Fran?ois A,Simon E,2001.Ectomycorrhizas and molecular phylogeny of the hypogeous russuloid fungus Arcangeliella borziana[J].Mycological Research,105(10):1231-1238
[22]Shen Q,Chen W,Yan Z,Xie Z,2009.Potential pharmaceutical resources of the Qinling Mountain in central China:medicinal fungi[J].Frontiers of Biology in China,4(1):89-93
[23]Kibar B,Peksen A,2011.Mycelial growth requirements of Lactarius pyrogalus and Lactarius controversus[J].Afr.J.Microbiol.Res,5(28):5107-5114
[24]Reddy S,2015.Diversity and Applications of Mushrooms[J].Plant Biology and Biotechnology,231-261
[25]Xu M L,Zhu J J,Kang H Z,Xu A H,Zhang J X,Li F Q,2008.Optimum conditions for pure culture of major ectomycorrhizal fungi obtained from Pinus sylvestris var.mongolica plantations in southeastern Keerqin sandy lands[J].China.Journal of Forestry Research,19(2):113-118
[26]任嘉红,班虎栋,叶建仁,2010.吡咯伯克霍尔德氏菌JK-SH007的发酵条件及其对杨树溃疡病的防治效果[J].中国生物防治,26(3):300-306
[27]Ren J H,Ye J R,Liu H,Xu X L,Wu X Q,2011.Isolation and characterization of a new Burkholderia pyrrocinia strain JK-SH007 as a potential biocontrol agent[J].World Journal of Microbiology and Biotechnology,27(9):2203-2215
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