CLOCK基因多态性与抑郁症患者睡眠障碍的关联研究

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• 论文作者：高琪 ; 张志珺 ; 徐治 ; 史艳艳 ; 耿磊钰 ; 浦梦佳
• 年：2016
• 作者机构：南京医科大学附属淮安市第一人民医院神经内科;东南大学医学院神经精神病学研究所;
• 论文关键词：抑郁症 ; CLOCK ; 基因多态性 ; 睡眠障碍
• 英文论文关键词：depression ; CLOCK ; gene polymorphism ; sleep disorders
• 会议召开时间：2016-11-25
• 会议录名称：湖南中医药大学学报2016/专集：国际数字医学会数字中医药分会成立大会暨首届数字中医药学术交流会论文集
• 语种：中文
• 分类号：R749.4
• 学会代码：HNZY
• 会议名称：国际数字医学会数字中医药分会成立大会暨首届数字中医药学术交流会
• 会议地点：中国广东珠海
• 主办单位：国际数字医学会、Digital Chinese Medicine
• 学会名称：湖南中医药大学期刊杂志社
• 页数：1
• 文件大小：2017k
• 原文格式：D
• 会议级别：全国

摘要

目的分析CLOCK基因rs11133399多态性与抑郁症患者睡眠障碍关系,进一步探讨其多态性在抑郁症发病遗传机制中的作用。方法入组281例抑郁症患者,采用汉密尔顿17项抑郁量表(HAMD)评定睡眠障碍,将入组患者分为有睡眠障碍组(HAMD睡眠因子分>0分)和无睡眠障碍组(HAMD睡眠因子分=0分)。采用基因芯片检测CLOCK基因rs11133399,运用SPSS 16.0软件包对有睡眠障碍组和无睡眠障碍组之间的人口学资料进行t检验和Pearson’sχ2检验。P<0.05为差异具有统计学意义。结果无睡眠障碍组中rs11133399位点AG基因型的分布频率显著高于有睡眠障碍组(F=7.060,P=0.029)结论本研究提示CLOCK基因位点rs11133399多态性可能与抑郁症患者睡眠障碍有关。
Objective To determine the association of SNP rs11133399 in CLOCK gene with sleep disorder, to explore whether CLOCK gene is the susceptibility genes of sleep disorders indepressive patients, and to investigate the role of the polymorphism in pathogenesis of depression. Methods We recruited 281 patients of major depressive disorder(MDD) who met the diagnosiscriteria of MDD(DSM-IV). Using the 17 Hamilton Depression Scale(HAMD-17) to assess sleep disorders.The patients were divided into sleep disorder group(score of sleep factor in HAMD >0) and nonsleep disorder group(score of sleep factor in HAMD =0).Using Illumina Golden Gate genotyping to determine the SNP rs11133399 of the CLOCK gene. The statistical analysis has been finished by SPSSpackage software for Windows version 16.0. Results The frequency of rs11133399 genotype AG in non sleep disorder group was significantly higher than in sleep disorder group(F= 7.060, P=0.029). Conclusion SNPs rs11133399 of CLOCK gene may be associated with sleep disorders of depressive patients.

引文

[1]Wirz-Justice A.Biological rhythm disturbances in mood disorders.International clinical psychopharmacology.2006;21 Suppl 1:S11-15.
    [2]Kato T.Molecular genetics of bipolar disorder and depression.Psychiatry and clinical neurosciences.2007;61(1):3-19.
    [3]Mc Clung CA.Circadian genes,rhythms and the biology of mood disorders.Pharmacology&therapeutics.2007;114(2):222-232.
    [4]Tsuno N,Besset A,Ritchie K.Sleep and depression.The Journal of clinical psychiatry.2005;66(10):1254-1269
    [5]Allebrandt KV,Teder-Laving M,Akyol M,et al.CLOCK gene variants associate with sleep duration in two independent populations.Biological psychiatry.2010;67(11):1040-1047
    [6]Benedetti F,Serretti A,Colombo C,et al.Influence of CLOCK gene polymorphism on circadian mood fluctuation and illness recurrence in bipolar depression.American journal of medical genetics.Part B,Neuropsychiatric genetics:the official publication of the International Society of Psychiatric Genetics.2003;123B(1):23-26
    [7]Lamont EW,James FO,Boivin DB,Cermakian N.From circadian clock gene expression to pathologies.Sleep medicine.2007;8(6):547-556.
    [8]Shiromani PJ,Xu M,Winston EM,Shiromani SN,Gerashchenko D,Weaver DR.Sleep rhythmicity and homeostasis in mice with targeted disruption of m Period genes.American journal of physiology.Regulatory,integrative and comparative physiology.2004;287(1):R47-57
    [9]Garaulet M,Lee YC,Shen J,et al.CLOCK genetic variation and metabolic syndrome risk:modulation by monounsaturated fatty acids.The American journal of clinical nutrition.2009;90(6):1466-1475.
    [10]Sookoian S,Gemma C,Gianotti TF,Burgueno A,Castano G,Pirola CJ.Genetic variants of Clock transcription factor are associated with individual susceptibility to obesity.The American journal of clinical nutrition.2008;87(6):1606-1615.
    [11]Scott EM,Carter AM,Grant PJ.Association between polymorphisms in the Clock gene,obesity and the metabolic syndrome in man.International journal of obesity.2008;32(4):658-662.
    [12]Gomez-Abellan P,Hernandez-Morante JJ,Lujan JA,Madrid JA,Garaulet M.Clock genes are implicated in the human metabolic syndrome.International journal of obesity.2008;32(1):121-128.