宁夏枸杞大孢子发生和雌配子体发育的半薄切片观察研究
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- 英文论文题名：Semi-thin sectioning Observation of Megasporogensis and female gametophyte(FG) in Lycium barbarum
- 论文作者：杨淑娟 ; 彭励 ; 毛桂莲
- 英文论文作者：Yang ShuJuan ; Peng Li ; Mao Guilian ; School of Life Sciences ; Ningxia University
- 年：2017
- 作者机构：宁夏大学生命科学学院;
- 论文关键词：宁夏枸杞 ; 大孢子发生 ; 雌配子体 ; 丝状器 ; 承珠盘
- 英文论文关键词：Lycium barbarum ; Megasporogenesis ; Female gametophyte ; filiform apparatus ; hypostase
- 会议召开时间：2017-01-11
- 会议录名称：2017全国植物生物技术发展与植物逆境生理研究前沿动态研讨会论文集
- 语种：中文
- 分类号：S567.19
- 学会代码：BJHS
- 会议名称：2017全国植物生物技术发展与植物逆境生理研究前沿动态研讨会
- 会议地点：中国海南海口
- 主办单位：中国农业发展与科技交流中心
- 学会名称：北京锦升会议服务中心
- 页数：10
- 文件大小：1782k
- 原文格式：D
- 会议级别：全国
摘要
采用半薄切片及组织化学染色法对宁夏枸杞大孢子发生和雌配子体发育过程中细胞结构及营养物质积累特征进行了观察。结果表明,枸杞为多室子房,倒生胚珠,多胚珠,单珠被,薄珠心类型;单孢原,位于珠心表皮下;直线形大孢子四分体,合点端的一个大孢子发育为功能大孢子,胚囊发育类型为蓼型,具有珠被绒毡层结构;刚形成的胚囊没外周没有物质积累,成熟胚囊时期出现了大量的淀粉粒,同时可见助细胞的珠孔端出现丝状器,而且呈PAS正反应,呈现出多糖性质。成熟胚囊具有承珠盘结构。
Megaspore and female gametophyte development were observed by using semi-thin sectioning and histochemical methods in Lycium barbarum.The results showed that the Lycium barbarum spore original cells can be directly as the megaspore mother cell,then through megaspore mother cell meiosis dyad formation,and then forming the linear sequence of four megaspore,chalaza end megaspore develops into functional megaspore.Ovules attached to the central axis of an ovary with more locules.Embryo sac development smartweed and mature embryo sac is composed of 7 cells.The ovule is of anatropous,simple integument and tenuinucellus。The mature embryo sac began to accumulate starch grains The mature embryo sac is surrounded by en lothelium tapetum.It is composed or an egg apparatus,one central cell with secondary nucleus,and 3 antipodal cells.the synergid contains the filiform apparatus.At the mature embryo sac stage,some nucellar cells at the chalazal region differentiated into hypostase.
Megaspore and female gametophyte development were observed by using semi-thin sectioning and histochemical methods in Lycium barbarum.The results showed that the Lycium barbarum spore original cells can be directly as the megaspore mother cell,then through megaspore mother cell meiosis dyad formation,and then forming the linear sequence of four megaspore,chalaza end megaspore develops into functional megaspore.Ovules attached to the central axis of an ovary with more locules.Embryo sac development smartweed and mature embryo sac is composed of 7 cells.The ovule is of anatropous,simple integument and tenuinucellus。The mature embryo sac began to accumulate starch grains The mature embryo sac is surrounded by en lothelium tapetum.It is composed or an egg apparatus,one central cell with secondary nucleus,and 3 antipodal cells.the synergid contains the filiform apparatus.At the mature embryo sac stage,some nucellar cells at the chalazal region differentiated into hypostase.
引文
1.Arroyo-Martinez Q.,Saenz,M.J.,Arguelles Arias,F.,and Acosta,M.S.Lycium barbarum:a new hepatotoxic"natural"agent?Dig Liver Dis,2011.43(9):749.
2.Cassileth B.Lycium(Lycium barbarum).Oncology(Williston Park),2010.24(14):1353.
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4.田惠桥.宁夏枸杞的大、小孢子发生和雌、雄配子体发育.武汉植物学研究.1987.17-24.
5.徐青.宁夏枸杞发育生物学.2009:黄河出版传媒集团,宁夏人民出版社.
6.申家恒,李伟,杨虹,丁建庭,李冉俐.辣椒大、小孢子发生与雌、雄配子体发育的研究.园艺学报.2007.1443-1452.
7.吴晓琛.辣椒胚珠的组织化学及卵器钙离子分布特征的研究,2014,厦门大学.
8.胡适宜.被子植物生殖生物学.2005.106-133.
9.Webb M.C.,and Gunning,B.E.S..Embryo sac development in Arabidopsis thaliana.I.Megasporogenesis,including the microtubular cytoskeleton.Sex.Plant Reprod,1990.3:244-256.
10.Russell S.D.Fine structure of megagametophyte development in Zea mays.Can.J.Bot,1979(57):1093-1110.
11.杨春雪,申家恒.星星草大、小孢子发生与雌、雄配子体发育的观察.武汉植物学研究,2003,06:464-470.
12.Demesa-Arevalo E.and Vielle-Calzada,J.P.The classical arabinogalactan protein AGP18 mediates megaspore selection in Arabidopsis.Plant Cell,2013.25(4):1274-1287.
13.Higashiyama T.,Yabe,S.,Sasaki,N.,Nishimura,Y.,Miyagishima,S.,Kuroiwa,H.,and Kuroiwa,T.Pollen tube attraction by the synergid cell.Science,2001.293(5534):1480-1483.
14.Higashiyama T.The synergid cell:attractor and acceptor of the pollen tube for double fertilization.JPlant Res,2002.115(1118):149-160.
15.Kasahara R.D.,Portereiko,M.F.,Sandaklie-Nikolova,L.,Rabiger,D.S.,and Drews,G.N.MYB98 is required for pollen tube guidance and synergid cell differentiation in Arabidopsis.Plant Cell,2005.17(11):2981-2992.
16.Punwani J.A.,Rabiger,D.S.,and Drews,G.N.MYB98 positively regulates a battery of synergid-expressed genes encoding filiform apparatus localized proteins.Plant Cell,2007.19(8):2557-2568.
17.施建军,张文红,杨静,孟源.紫荆(Cercischinensis Bunge)大孢子发生及雌配子体发育的研究.中国工业经济.2012.123-134.
18.张雪,慕小倩,蔡宇良,冯瑛,宛甜.‘马哈利’樱桃雌雄蕊发育解剖学研究.果树学报,2014(S1):57-63.
19.赵桦.丫蕊花胚胎发育的研究I.大孢子,雌配子体的发生及淀粉粒的动态.四川大学学报.1985.(4):183-191.
20.W HESSE M,HESS M.Recent trends in tapetum research.Acytological and methodological review.Pl Syst Evol,1993.7:127-145.
21.Russell S.D.and Mao,L.J.Patterns of embryo-sac organization,synergid degeneration and cotyledon orientation in Linum usitatissimum L.Planta,1990.182(1):52-57.
22.Leshem Y.,Johnson,C.,and Sundaresan,V.Pollen tube entry into the synergid cell of Arabidopsis is observed at a site distinct from the filiform apparatus.Plant Reprod,2013.26(2):93-99.
23.李晶,郑彩霞.高等植物珠被绒毡层的研究.植物生理学报.2012,48(12):1135-1140.
24.Valtue?a FJ Rodríguez-Ria?oa T,Ortega-Olivencia A.Ephem-eral and non-ephemeral structures during seed development in two Cytisus species(Papilionoideae,Leguminosae).Plant Biosyst,2011.145(1):98-105.
25.曲波,许玉凤,邵美妮,翟强,张春宇,李冬华.虎眼万年青胚珠及胚囊发育的研究.2004.35(1).沈阳农业大学学报.7-9.
2.Cassileth B.Lycium(Lycium barbarum).Oncology(Williston Park),2010.24(14):1353.
3.李文钿,王锡林,罗蕴芳.宁夏枸杞开花结果形态发育的初步观察.宁夏农业科技.1979.32-35.
4.田惠桥.宁夏枸杞的大、小孢子发生和雌、雄配子体发育.武汉植物学研究.1987.17-24.
5.徐青.宁夏枸杞发育生物学.2009:黄河出版传媒集团,宁夏人民出版社.
6.申家恒,李伟,杨虹,丁建庭,李冉俐.辣椒大、小孢子发生与雌、雄配子体发育的研究.园艺学报.2007.1443-1452.
7.吴晓琛.辣椒胚珠的组织化学及卵器钙离子分布特征的研究,2014,厦门大学.
8.胡适宜.被子植物生殖生物学.2005.106-133.
9.Webb M.C.,and Gunning,B.E.S..Embryo sac development in Arabidopsis thaliana.I.Megasporogenesis,including the microtubular cytoskeleton.Sex.Plant Reprod,1990.3:244-256.
10.Russell S.D.Fine structure of megagametophyte development in Zea mays.Can.J.Bot,1979(57):1093-1110.
11.杨春雪,申家恒.星星草大、小孢子发生与雌、雄配子体发育的观察.武汉植物学研究,2003,06:464-470.
12.Demesa-Arevalo E.and Vielle-Calzada,J.P.The classical arabinogalactan protein AGP18 mediates megaspore selection in Arabidopsis.Plant Cell,2013.25(4):1274-1287.
13.Higashiyama T.,Yabe,S.,Sasaki,N.,Nishimura,Y.,Miyagishima,S.,Kuroiwa,H.,and Kuroiwa,T.Pollen tube attraction by the synergid cell.Science,2001.293(5534):1480-1483.
14.Higashiyama T.The synergid cell:attractor and acceptor of the pollen tube for double fertilization.JPlant Res,2002.115(1118):149-160.
15.Kasahara R.D.,Portereiko,M.F.,Sandaklie-Nikolova,L.,Rabiger,D.S.,and Drews,G.N.MYB98 is required for pollen tube guidance and synergid cell differentiation in Arabidopsis.Plant Cell,2005.17(11):2981-2992.
16.Punwani J.A.,Rabiger,D.S.,and Drews,G.N.MYB98 positively regulates a battery of synergid-expressed genes encoding filiform apparatus localized proteins.Plant Cell,2007.19(8):2557-2568.
17.施建军,张文红,杨静,孟源.紫荆(Cercischinensis Bunge)大孢子发生及雌配子体发育的研究.中国工业经济.2012.123-134.
18.张雪,慕小倩,蔡宇良,冯瑛,宛甜.‘马哈利’樱桃雌雄蕊发育解剖学研究.果树学报,2014(S1):57-63.
19.赵桦.丫蕊花胚胎发育的研究I.大孢子,雌配子体的发生及淀粉粒的动态.四川大学学报.1985.(4):183-191.
20.W HESSE M,HESS M.Recent trends in tapetum research.Acytological and methodological review.Pl Syst Evol,1993.7:127-145.
21.Russell S.D.and Mao,L.J.Patterns of embryo-sac organization,synergid degeneration and cotyledon orientation in Linum usitatissimum L.Planta,1990.182(1):52-57.
22.Leshem Y.,Johnson,C.,and Sundaresan,V.Pollen tube entry into the synergid cell of Arabidopsis is observed at a site distinct from the filiform apparatus.Plant Reprod,2013.26(2):93-99.
23.李晶,郑彩霞.高等植物珠被绒毡层的研究.植物生理学报.2012,48(12):1135-1140.
24.Valtue?a FJ Rodríguez-Ria?oa T,Ortega-Olivencia A.Ephem-eral and non-ephemeral structures during seed development in two Cytisus species(Papilionoideae,Leguminosae).Plant Biosyst,2011.145(1):98-105.
25.曲波,许玉凤,邵美妮,翟强,张春宇,李冬华.虎眼万年青胚珠及胚囊发育的研究.2004.35(1).沈阳农业大学学报.7-9.
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