<正>Background:Newly developed RNA capture and profiling assays like Ch IRP sonicate RNAs into pieces of a few hundred nucleotides and then use biotin-labeled oligonucleotide probes to retrieve targetRNAs from cell lysate.Existing methods usually only design probes in non-homologous regions of targetRNAs,often resulting in big gaps(>500nt)without any probe coverage.Since RNA together with