作物高油品种选育策略研究进展
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摘要
作物高油品种培育是育种工作的目标之一,对油脂合成过程中一些关键基因进行鉴定和克隆可以加速作物高油品种的选育过程。归纳了植物体内油脂合成的通路和调控网络,从碳源分配、脂肪酸从头合成、三酰甘油的合成效率3个方面总结了植物油脂合成的关键步骤,并对这3个过程中关键基因的研究进展进行了归纳总结,为后期作物高油品种和特殊油脂品种的选育提供技术指导和理论支撑。
Cultivating the high oil crop varieties is one of the breeding objectives.Identification and cloning of some key genes related lipid synthesis could accelerate the breeding process of high crop oil varieties.In this paper,the pathways and regulatory networks of lipid biosynthesis in plant were concluded,the key steps of plant lipid synthesis were summarized from three aspects which were carbon source allocation,de novo synthesis of fatty acid and the efficiency of triacylgcerol synthesis,as well as the key genes of the three pathways were summarized.This work would provide a technical guidance and theoretical support for the breeding of high oil crop varieties and special oil crop varieties.
Cultivating the high oil crop varieties is one of the breeding objectives.Identification and cloning of some key genes related lipid synthesis could accelerate the breeding process of high crop oil varieties.In this paper,the pathways and regulatory networks of lipid biosynthesis in plant were concluded,the key steps of plant lipid synthesis were summarized from three aspects which were carbon source allocation,de novo synthesis of fatty acid and the efficiency of triacylgcerol synthesis,as well as the key genes of the three pathways were summarized.This work would provide a technical guidance and theoretical support for the breeding of high oil crop varieties and special oil crop varieties.
引文
[1] 赵彦朋,梁伟,王丹,等.植物油脂合成调控与遗传改良研究进展[J].中国农业科技导报,2018,20(1):14-24.
[2] GUO Y L,HUANG Y,GAO J,et al.CIPK9 is involved in seed oil regulation in Brassica napus L.and Arabidopsis thaliana (L.) Heynh[J].Biotechnology for Biofuels,2018,11(1):124.
[3] HU Q,HUA W,YIN Y,et al.Rapeseed research and production in China[J].The Crop Journal,2017,5(2):127-135.
[4] 郭陞垚,陈剑洪,詹柳琪,等.福建省高油酸花生新品系比较试验[J].福建农业科技,2018(1):7-9.
[5] TRONCOSO-PONCE M A,NIKOVICS K,MARCHIVE C,et al.New insights on the organization and regulation of the fatty acid biosynthetic network in the model higher plant Arabidopsis thaliana[J].Biochimie,2016,120:3-8.
[6] LIU H L,SHEN H T,CHEN C,et al.Identification of a putative stearoyl acyl-carrier-protein desaturase gene from Saussurea involucrata[J].Biologia Plantarum,2015,59(2):316-324.
[7] ZALE J,JUNG J H,KIM J Y,et al.Metabolic engineering of sugarcane to accumulate energy-dense triacylglycerols in vegetative biomass[J].Plant Biotechnology Journal,2016,14(2):661-669.
[8] 何思思,王元丽,高保燕,等.微藻三酰甘油合成途径及其最新研究进展[J].生命科学,2014,26(9):979-990.
[9] 张照华,王志慧,淮东欣,等.利用回交和标记辅助选择快速培育高油酸花生品种及其评价[J].中国农业科学,2018,51(9):1641-1652.
[10] 陈四龙,程增书,王瑾,等.一个新的高油酸花生突变体的鉴定与育种应用[C]//中国作物学会.中国作物学会油料作物专业委员会第八次会员代表大会暨学术年会综述与摘要集.北京:[出版者不详],2018.
[11] 殷冬梅,杨秋云,杨海棠,等.花生突变体的EMS诱变及分子检测[J].中国农学通报,2009,25(5):53-56.
[12] 王传堂,王秀贞,唐月异,等.中国高油酸花生种质创制、品种选育进展与建议[J].花生学报,2015,44(2):49-53.
[13] LU S P,STURTEVANT D,AZIZ M,et al.Spatial analysis of lipid metabolites and expressed genes reveals tissue-specific heterogeneity of lipid metabolism in high- and low-oil Brassica napus L.seeds[J].Plant Journal,2018,94(6):915-932.
[14] 王艳芳,苏婉玉,曹绍玉,等.新型基因编辑技术发展及在植物育种中的应用[J].西北农业学报,2018,27(5):617-625.
[15] 陈锦清,郎春秀,胡张华,等.反义PEP基因调控油菜籽粒蛋白质/油脂含量比率的研究[J].农业生物技术学报,1999,7(4):316-320.
[16] VAZQUEZ-TELLO A,WHITTIER R F,KAWASAKI T,et al.Sequence of a soybean(Glycine max L.)phosphoenolpyruvate carboxylase cDNA[J].Plant Physiology,1993,103(3):1025-1026.
[17] 赵翠格,刘頔,李凤兰,等.植物种子油脂的生物合成及代谢基础研究进展[J].种子,2010,29(4):56-62.
[18] XU Z P,LI J W,GUO X P,et al.Metabolic engineering of cottonseed oil biosynthesis pathway via RNA interference[J].Scientific Reports,2016,6:33342.
[19] ZHAO Y P,HUANG Y,WANG Y M,et al.RNA interference of GhPEPC2 enhanced seed oil accumulation and salt tolerance in upland cotton[J].Plant Science,2018,271:52-61.
[20] MEYER K,STECCA K L,EWELL-HICKS K,et al.Oil and protein accumulation in developing seeds is influenced by the expression of a cytosolic pyrophosphatase in Arabidopsis[J].Plant Physiology,2012,159(3):1221-1234.
[21] PAN L,ZHANG J,CHI X,et al.The antisense expression of AhPEPC1 increases seed oil production in peanuts (Arachis hypogaea L.)[J].Grasas Y Aceites,2016,67(4):e164.
[22] 闫博巍,许晓萱,谷英楠,等.玉米Ⅰ型二酰基转移酶基因(DAGT1)的生物信息学分析及其在非生物胁迫下的表达研究[J].玉米科学,2018,26(1):20-28.
[23] MCGLEW K,SHAW V,ZHANG M,et al.An annotated database of Arabidopsis mutants of acyl lipid metabolism[J].Plant Cell Reports,2015,34(4):519-532.
[24] SONG L,LIU D.Mutations in the three Arabidopsis genes that encode the E2 subunit of the mitochondrial pyruvate dehydrogenase complex differentially affect enzymatic activity and plant growth[J].Plant Cell Reports,2015,34(11):1919-1926.
[25] YU H L,DU X Q,ZHANG F X,et al.A mutation in the E2 subunit of the mitochondrial pyruvate dehydrogenase complex in Arabidopsis reduces plant organ size and enhances the accumulation of amino acids and intermediate products of the TCA cycle[J].Planta,2012,236(2):387-399.
[26] BROZ A K,TOVAR-MéNDEZ A,MOONEY B P,et al.A novel regulatory mechanism based upon a dynamic core structure for the mitochondrial pyruvate dehydrogenase complex?[J].Mitochondrion,2014,19:144-153.
[27] CUI Y P,LIU Z J,ZHAO Y P,et al.Overexpression of heteromeric GhACCase subunits enhanced oil accumulation in upland cotton[J].Plant Molecular Biology Reporter,2017,35(2):287-297.
[28] BI Y L,LIU W T,GUO W L,et al.Molecular basis of multiple resistance to ACCase- and ALS-inhibiting herbicides in Alopecurus japonicus from China[J].Pestic Biochem and Physiol,2016,126:22-27.
[29] SHERGILL L S,FLEET B,PRESTON C A,et al.Incidence of herbicide resistance,seedling emergence,and seed persistence of smooth barley(Hordeum glaucum) in South Australia[J].Weed Technology,2015,29(4):782-792.
[30] STOLL C,LüHS W,ZARHLOUL M K,et al.Knockout of KASⅢ regulation changes fatty acid composition in canola(Brassica napus)[J].European Journal of Lipid Science and Technology,2010,108(4):277-286.
[31] XIONG W D,WEI Q,WU P Z,et al.Molecular cloning and characterization of two β-ketoacyl-acyl carrier protein synthaseⅠgenes from Jatropha curcas L.[J].Journal of Plant Physiology,2017,214:152-160.
[32] OZSEYHAN M E,LI P C,NA G N,et al.Improved fatty acid profiles in seeds of Camelina sativa by artificial microRNA mediated FATB gene suppression[J].Biochemical and Biophysical Research Communications,2018,503(2):621-624.
[33] AZNAR-MORENO J A,VENEGAS-CALERóN M,MARTíNEZ-FORCE E,et al.Acyl carrier proteins from sunflower(Helianthus annuus L.) seeds and their influence on FatA and FatB acyl-ACP thioesterase activities[J].Planta,2016,244(2):479-490.
[34] SHOCKEY J,REGMI A,COTTON K,et al.Identification of Arabidopsis GPAT9(At5g60620) as an essential gene involved in triacylglycerol biosynthesis[J].Plant Physiology,2015,170(1):163.
[35] XU J Y,FRANCIS T,MIETKIEWSKA E,et al.Cloning and characterization of an acyl-CoA-dependent diacylglycerol acyltransferase 1(DGAT1) gene from Tropaeolum majus,and a study of the functional motifs of the DGAT protein using site-directed mutagenesis to modify enzyme activity and oil content[J].Plant Biotechnology Journal,2008,6(8):799-818.
[36] ROSLI R,CHAN P L,CHAN K L,et al.In silico characterization and expression profiling of the diacylglycerol acyltransferase gene family(DGAT1,DGAT2,DGAT3 and WS/DGAT) from oil palm,Elaeis guineensis[J].Plant Science,2018,275:84-96.
[37] WANG Y Q,PENG D,ZHANG L,et al.Overexpression of SsDGAT2 from Sapium sebiferum(L.) Roxb increases seed oleic acid level in Arabidopsis[J].Plant Molecular Biology Reporter,2016,34(3):638-648.
[38] PENG D,ZHANG L,TAN X F,et al.Increasing seed oil content and altering oil quality of Brassica napus L.by over-expression of diacylglycerol acyltransferase 1(SsDGAT1) from Sapium sebiferum(L.) Roxb[J].Molecular Breeding,2016,36(10):136.
[39] MARMON S,STURTEVANT D,HERRFURTH C,et al.Two acyltransferases contribute differently to linolenic acid levels in seed oil[J].Plant Physiology,2017,173(4):2081-2095.
[40] YUAN L X,MAO X,ZHAO K,et al.Characterisation of phospholipid:Diacylglycerol acyltransferases(PDATs) from Camelina sativa and their roles in stress responses[J].Biology Open,2017,6(7):1024-1034.
[2] GUO Y L,HUANG Y,GAO J,et al.CIPK9 is involved in seed oil regulation in Brassica napus L.and Arabidopsis thaliana (L.) Heynh[J].Biotechnology for Biofuels,2018,11(1):124.
[3] HU Q,HUA W,YIN Y,et al.Rapeseed research and production in China[J].The Crop Journal,2017,5(2):127-135.
[4] 郭陞垚,陈剑洪,詹柳琪,等.福建省高油酸花生新品系比较试验[J].福建农业科技,2018(1):7-9.
[5] TRONCOSO-PONCE M A,NIKOVICS K,MARCHIVE C,et al.New insights on the organization and regulation of the fatty acid biosynthetic network in the model higher plant Arabidopsis thaliana[J].Biochimie,2016,120:3-8.
[6] LIU H L,SHEN H T,CHEN C,et al.Identification of a putative stearoyl acyl-carrier-protein desaturase gene from Saussurea involucrata[J].Biologia Plantarum,2015,59(2):316-324.
[7] ZALE J,JUNG J H,KIM J Y,et al.Metabolic engineering of sugarcane to accumulate energy-dense triacylglycerols in vegetative biomass[J].Plant Biotechnology Journal,2016,14(2):661-669.
[8] 何思思,王元丽,高保燕,等.微藻三酰甘油合成途径及其最新研究进展[J].生命科学,2014,26(9):979-990.
[9] 张照华,王志慧,淮东欣,等.利用回交和标记辅助选择快速培育高油酸花生品种及其评价[J].中国农业科学,2018,51(9):1641-1652.
[10] 陈四龙,程增书,王瑾,等.一个新的高油酸花生突变体的鉴定与育种应用[C]//中国作物学会.中国作物学会油料作物专业委员会第八次会员代表大会暨学术年会综述与摘要集.北京:[出版者不详],2018.
[11] 殷冬梅,杨秋云,杨海棠,等.花生突变体的EMS诱变及分子检测[J].中国农学通报,2009,25(5):53-56.
[12] 王传堂,王秀贞,唐月异,等.中国高油酸花生种质创制、品种选育进展与建议[J].花生学报,2015,44(2):49-53.
[13] LU S P,STURTEVANT D,AZIZ M,et al.Spatial analysis of lipid metabolites and expressed genes reveals tissue-specific heterogeneity of lipid metabolism in high- and low-oil Brassica napus L.seeds[J].Plant Journal,2018,94(6):915-932.
[14] 王艳芳,苏婉玉,曹绍玉,等.新型基因编辑技术发展及在植物育种中的应用[J].西北农业学报,2018,27(5):617-625.
[15] 陈锦清,郎春秀,胡张华,等.反义PEP基因调控油菜籽粒蛋白质/油脂含量比率的研究[J].农业生物技术学报,1999,7(4):316-320.
[16] VAZQUEZ-TELLO A,WHITTIER R F,KAWASAKI T,et al.Sequence of a soybean(Glycine max L.)phosphoenolpyruvate carboxylase cDNA[J].Plant Physiology,1993,103(3):1025-1026.
[17] 赵翠格,刘頔,李凤兰,等.植物种子油脂的生物合成及代谢基础研究进展[J].种子,2010,29(4):56-62.
[18] XU Z P,LI J W,GUO X P,et al.Metabolic engineering of cottonseed oil biosynthesis pathway via RNA interference[J].Scientific Reports,2016,6:33342.
[19] ZHAO Y P,HUANG Y,WANG Y M,et al.RNA interference of GhPEPC2 enhanced seed oil accumulation and salt tolerance in upland cotton[J].Plant Science,2018,271:52-61.
[20] MEYER K,STECCA K L,EWELL-HICKS K,et al.Oil and protein accumulation in developing seeds is influenced by the expression of a cytosolic pyrophosphatase in Arabidopsis[J].Plant Physiology,2012,159(3):1221-1234.
[21] PAN L,ZHANG J,CHI X,et al.The antisense expression of AhPEPC1 increases seed oil production in peanuts (Arachis hypogaea L.)[J].Grasas Y Aceites,2016,67(4):e164.
[22] 闫博巍,许晓萱,谷英楠,等.玉米Ⅰ型二酰基转移酶基因(DAGT1)的生物信息学分析及其在非生物胁迫下的表达研究[J].玉米科学,2018,26(1):20-28.
[23] MCGLEW K,SHAW V,ZHANG M,et al.An annotated database of Arabidopsis mutants of acyl lipid metabolism[J].Plant Cell Reports,2015,34(4):519-532.
[24] SONG L,LIU D.Mutations in the three Arabidopsis genes that encode the E2 subunit of the mitochondrial pyruvate dehydrogenase complex differentially affect enzymatic activity and plant growth[J].Plant Cell Reports,2015,34(11):1919-1926.
[25] YU H L,DU X Q,ZHANG F X,et al.A mutation in the E2 subunit of the mitochondrial pyruvate dehydrogenase complex in Arabidopsis reduces plant organ size and enhances the accumulation of amino acids and intermediate products of the TCA cycle[J].Planta,2012,236(2):387-399.
[26] BROZ A K,TOVAR-MéNDEZ A,MOONEY B P,et al.A novel regulatory mechanism based upon a dynamic core structure for the mitochondrial pyruvate dehydrogenase complex?[J].Mitochondrion,2014,19:144-153.
[27] CUI Y P,LIU Z J,ZHAO Y P,et al.Overexpression of heteromeric GhACCase subunits enhanced oil accumulation in upland cotton[J].Plant Molecular Biology Reporter,2017,35(2):287-297.
[28] BI Y L,LIU W T,GUO W L,et al.Molecular basis of multiple resistance to ACCase- and ALS-inhibiting herbicides in Alopecurus japonicus from China[J].Pestic Biochem and Physiol,2016,126:22-27.
[29] SHERGILL L S,FLEET B,PRESTON C A,et al.Incidence of herbicide resistance,seedling emergence,and seed persistence of smooth barley(Hordeum glaucum) in South Australia[J].Weed Technology,2015,29(4):782-792.
[30] STOLL C,LüHS W,ZARHLOUL M K,et al.Knockout of KASⅢ regulation changes fatty acid composition in canola(Brassica napus)[J].European Journal of Lipid Science and Technology,2010,108(4):277-286.
[31] XIONG W D,WEI Q,WU P Z,et al.Molecular cloning and characterization of two β-ketoacyl-acyl carrier protein synthaseⅠgenes from Jatropha curcas L.[J].Journal of Plant Physiology,2017,214:152-160.
[32] OZSEYHAN M E,LI P C,NA G N,et al.Improved fatty acid profiles in seeds of Camelina sativa by artificial microRNA mediated FATB gene suppression[J].Biochemical and Biophysical Research Communications,2018,503(2):621-624.
[33] AZNAR-MORENO J A,VENEGAS-CALERóN M,MARTíNEZ-FORCE E,et al.Acyl carrier proteins from sunflower(Helianthus annuus L.) seeds and their influence on FatA and FatB acyl-ACP thioesterase activities[J].Planta,2016,244(2):479-490.
[34] SHOCKEY J,REGMI A,COTTON K,et al.Identification of Arabidopsis GPAT9(At5g60620) as an essential gene involved in triacylglycerol biosynthesis[J].Plant Physiology,2015,170(1):163.
[35] XU J Y,FRANCIS T,MIETKIEWSKA E,et al.Cloning and characterization of an acyl-CoA-dependent diacylglycerol acyltransferase 1(DGAT1) gene from Tropaeolum majus,and a study of the functional motifs of the DGAT protein using site-directed mutagenesis to modify enzyme activity and oil content[J].Plant Biotechnology Journal,2008,6(8):799-818.
[36] ROSLI R,CHAN P L,CHAN K L,et al.In silico characterization and expression profiling of the diacylglycerol acyltransferase gene family(DGAT1,DGAT2,DGAT3 and WS/DGAT) from oil palm,Elaeis guineensis[J].Plant Science,2018,275:84-96.
[37] WANG Y Q,PENG D,ZHANG L,et al.Overexpression of SsDGAT2 from Sapium sebiferum(L.) Roxb increases seed oleic acid level in Arabidopsis[J].Plant Molecular Biology Reporter,2016,34(3):638-648.
[38] PENG D,ZHANG L,TAN X F,et al.Increasing seed oil content and altering oil quality of Brassica napus L.by over-expression of diacylglycerol acyltransferase 1(SsDGAT1) from Sapium sebiferum(L.) Roxb[J].Molecular Breeding,2016,36(10):136.
[39] MARMON S,STURTEVANT D,HERRFURTH C,et al.Two acyltransferases contribute differently to linolenic acid levels in seed oil[J].Plant Physiology,2017,173(4):2081-2095.
[40] YUAN L X,MAO X,ZHAO K,et al.Characterisation of phospholipid:Diacylglycerol acyltransferases(PDATs) from Camelina sativa and their roles in stress responses[J].Biology Open,2017,6(7):1024-1034.
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