受引黄影响的河流沉积物细菌群落季节变化
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摘要
河流沉积物细菌群落的季节变化对碳、氮循环和水环境演化有明显的影响.然而,受调水干预的河流沉积物细菌群落季节性变化尚不明确.本文基于16S rRNA高通量测序技术,研究了中纬度地区受引黄影响的汾河上游沉积物细菌群落组成、结构和多样性的季节变化.结果表明,在引黄调水干预下受水河流细菌群落的季节性变化仍然显著.夏季温度适宜,河流沉积物细菌群落多样性较冬季高.核心菌群组成和结构随季节变化,然而丛毛单胞菌、黄杆菌、黄单胞菌和红环菌等的优势地位不随季节改变.河水水温和ORP的季节变化显著,是造成河流沉积物细菌群落季节变化的主导因子.水质较差的黄河水引入汾河后导致沉积物细菌群落多样性显著提高.引水对河流沉积物细菌群落的影响在夏季较冬季高20%左右.引黄调水对汾河沉积物细菌群落的影响大于季节变化的影响.
Seasonal changes of bacterial communities in river sediments have obvious effects on carbon/nitrogen cycles and water environment evolution. However, the seasonal changes of the bacterial communities affected by water transfer have not been clear yet. Based on the 16 S rRNA high-throughput sequencing technique, we studied the seasonal variation of composition, structure and diversity of bacterial communities of river sediments in the upper reaches of the Fen River where the Yellow River water is transferred into. Results show that seasonal changes of bacterial communities are significant even under the influences of the water diversion. The summer temperature is suitable for the majority of bacteria. The bacterial community diversity in river sediments is higher in summer than that in winter. The composition and structure of the dominant bacteria group vary with the seasons, while the dominant communities of Comamonadaceae, Flavobacterium, Xanthomonadaceae and Rhodocyclaceae don′t change. The seasonal variations of river water temperature and oxidation-reduction potential(ORP) are the dominant factors that causes the seasonal change of bacterial communities in the river sediments. The diversity of bacterial communities in sediments increases significantly after the introduction of the Yellow River water with poor quality. The effect of water diversion on the bacterial communities of river sediments is about 20% higher in summer than in winter. The effect of water transfer on the bacterial communities in the Fen River′s sediments is greater than that of seasonal variation.
Seasonal changes of bacterial communities in river sediments have obvious effects on carbon/nitrogen cycles and water environment evolution. However, the seasonal changes of the bacterial communities affected by water transfer have not been clear yet. Based on the 16 S rRNA high-throughput sequencing technique, we studied the seasonal variation of composition, structure and diversity of bacterial communities of river sediments in the upper reaches of the Fen River where the Yellow River water is transferred into. Results show that seasonal changes of bacterial communities are significant even under the influences of the water diversion. The summer temperature is suitable for the majority of bacteria. The bacterial community diversity in river sediments is higher in summer than that in winter. The composition and structure of the dominant bacteria group vary with the seasons, while the dominant communities of Comamonadaceae, Flavobacterium, Xanthomonadaceae and Rhodocyclaceae don′t change. The seasonal variations of river water temperature and oxidation-reduction potential(ORP) are the dominant factors that causes the seasonal change of bacterial communities in the river sediments. The diversity of bacterial communities in sediments increases significantly after the introduction of the Yellow River water with poor quality. The effect of water diversion on the bacterial communities of river sediments is about 20% higher in summer than in winter. The effect of water transfer on the bacterial communities in the Fen River′s sediments is greater than that of seasonal variation.
引文
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邸琰茗,王广煊,黄兴如,等.2017.再生水补水对河道底泥细菌群落组成与功能的影响[J].环境科学,38(2):743-751
高大文,王淑莹,彭永臻,等.2003a.温度变化对DO和ORP作为过程控制参数的影响[J].环境科学,24(1):63-69
高大文,王淑莹,彭永臻,等.2003b.温度变化对DO和ORP作为过程控制参数的影响[J].环境科学,24(1):63-69
高慧琴,刘凌,方泽建.2011.夏季湖泊表层沉积物的理化性质与微生物多样性[J].河海大学学报(自然科学版),39(4):361-366
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Ginige M P,Hugenholtz P,Daims H,et al.2004.Use of stable-isotope probing,full-cycle rRNA analysis,and fluorescence in situ hybridization-microautoradiography to study a methanol-fed denitrifying microbial community[J].Appl Environ Microbiol,70(1):588-596
Heylen K,Lebbe L,De V P.2008.Acidovorax caeni sp.nov.,a denitrifying species with genetically diverse isolates from activated sludge[J].International Journal of Systematic & Evolutionary Microbiology,58(Pt 1):73
黄兴如,张琼琼,张瑞杰,等.2016.再生水补水对河流湿地香蒲根际细菌群落结构影响研究[J].中国环境科学,36(2):569-580
Ibekwe A M,Ma J,Murinda S E.2016.Bacterial community composition and structure in an Urban River impacted by different pollutant sources[J].Science of the Total Environment,566-567:1176-1185
江玉梅,张晨,黄小兰,等.2016.重金属污染对鄱阳湖底泥微生物群落结构的影响[J].中国环境科学,36(11):3475-3486
金笑,寇文伯,于昊天,等.2017.鄱阳湖不同区域沉积物细菌群落结构、功能变化及其与环境因子的关系[J].环境科学研究,30(4):529-536
Juretschko S,Loy A,Lehner A,et al.2002.The microbial community composition of a nitrifying-denitrifying activated sludge from an industrial sewage treatment plant analyzed by the full-cycle rRNA approach[J].Syst Appl Microbiol,25(1):84-99
Lee N,Nielsen P H,Aspegren H,et al.2003.Long-term population dynamics and in situ physiology in activated sludge systems with enhanced biological phosphorus removal operated with and without nitrogen removal[J].Systematic & Applied Microbiology,26(2):211
Liu J,Sui Y,Yu Z,et al.2014.High throughput sequencing analysis of biogeographical distribution of bacterial communities in the black soils of northeast China[J].Soil Biology & Biochemistry,70(2):113-122
李建洋,赵月,谢宁栋,等.2017.深圳近海表层浮游细菌分布特征及其环境影响因素[J].生态学报,37(24):8567-8577
Mills A L,Mallory L M.1987.The community structure of sessile heterotrophic bacteria stressed by acid mine drainage[J].Microbial Ecology,14(3):219-232
Morgan X C,Kabakchiev B,Waldron L,et al.2015.Associations between host gene expression,the mucosal microbiome,and clinical outcome in the pelvic pouch of patients with inflammatory bowel disease[J].Genome Biology,16(1):67
Purkhold U,Pommereningr?ser A,Juretschko S,et al.2000.Phylogeny of all recognized species of ammonia oxidizers based on comparative 16S rRNA and amoA sequence analysis:Implications for molecular diversity surveys[J].Appl.environ.microbiol,66(12):5368-5382
Quan X,Wang Y,Xiong W,et al.2010.Description of microbial community structure of sediments from the Daliao River water system and its estuary (NE China) by application of fluorescence in situ hybridization[J].Environmental Earth Sciences,61(8):1725-1734
阮宇鹰.2008.黑臭河水生物栅处理系统中填料及植物根系微生物生态研究[D].上海:华东师范大学
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Sadaie T,Sadaie A,Takada M,et al.2007.Reducing sludge production and the domination of comamonadaceae by reducing the oxygen supply in the wastewater treatment procedure of a food-processing factory[J].Journal of the Agricultural Chemical Society of Japan,71(3):791
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Sjostedt J,Koch-Schmidt P,Pontarp M,et al.2012.Recruitment of members from the rare biosphere of marine bacterioplankton communities after an environmental disturbance[J].Appl Environ Microbiol,78(5):1361-1369
佘跃惠,张凡,向廷生,等.2005.PCR-DGGE方法分析原油储层微生物群落结构及种群多样性[J].生态学报,25(2):237-242
沈烽,赵大勇,黄睿,等.2017.南京玄武湖浮游细菌群落结构的季节变化及其与环境因子的关系[J].湖泊科学,29(3):662-669
施邑屏.1982.温度与微生物[J].微生物学通报,9(6):41-44
唐婧,徐小蓉,商传禹,等.2015.南明河城区河段细菌多样性与环境因子的关系[J].微生物学报,55(8):1050-1059
屠腾,李蕾,毛冠男,等.2012.利用细胞计数手段和DGGE技术分析松花江干流部分地区的细菌种群多样性[J].生态学报,32(11):3505-3515
Wang G,Liu J,Qi X,et al.2008.Effects of fertilization on bacterial community structure and function in a black soil of Dehui region estimated by Biolog and PCR-DGGE methods[J].Acta Ecologica Sinica,28(1):220-226
Wang Z,Su Y,Zhang Y,et al.2016.Ecology-types determine physicochemical properties and microbial communities of sediments obtained along the Songhua River[J].Biochemical Systematics & Ecology,66(7):312-318
王彩霞,王毅波,刘鹏远,等.2018.渤海三湾表层水域细菌群落结构多样性及其环境因子分析[J].微生物学通报,(9):1956-1971
王佳.2015.浑河底泥微生物群落结构时空变化及其与水环境因子间的关联研究[D].西安:西安建筑科技大学.76
王佳,彭剑峰,宋永会,等.2016a.浑河底泥微生物群落的季节性变化特征[J].环境科学研究,29(2):202-210
王佳,彭剑峰,宋永会,等.2016b.浑河底泥微生物群落的季节性变化特征[J].环境科学研究,29(2):202-210
夏围围,贾仲君.2014.高通量测序和DGGE分析土壤微生物群落的技术评价[J].微生物学报,54(12):1489-1499
肖慧慧,倪晋仁.2013.城镇污水处理厂活性污泥细菌群落结构特征分析[J].应用基础与工程科学学报,21(3):522-531
徐华成,徐晓军,王凯,等.2007.饮用水氧化还原电位的影响因素分析[J].苏州科技学院学报(工程技术版)(2):63-66
薛俊增,肖南燕,王琼,等.2016.洋山港海域细菌群落多样性的季节变化[J].生态学报,36(23):7758-7767
Yu X Y,Li Y F,Zheng J W,et al.2011.Comamonas zonglianii sp.nov.,isolated from phenol-contaminated soil[J].Int J Syst Evol Microbiol,61(Pt 2):255-258
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