活血散结中药复方含药血浆对PDGF干预下兔RPE细胞增殖及IGF-Ⅰ表达的影响

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英文篇名：Effects of drug-containing plasma of Huoxue Sanjie Formula on rabbit RPE cell proliferation and IGF-Ⅰ expression under the PDGF intervention 作者：潘坤 ; 彭俊 ; 吴佳敏 ; 张又玮 ; 李建超 ; 吴权龙 ; 彭清华 英文作者：PAN Kun;PENG Jun;WU Jia-min;ZHANG You-wei;LI Jian-chao;WU Quan-long;PENG Qing-hua;Hunan University of Chinese Medicine; 关键词：活血散结中药复方 ; 增生性玻璃体视网膜病变 ; 视网膜色素上皮细胞 ; 细胞增殖 ; 胰岛素样生长因子-Ⅰ 英文关键词：Huoxue Sanjie Formula;;Proliferative vitreoretinopathy;;Retinal pigment epithelial cells;;Cell proliferation;;Insulin-like growth factor Ⅰ 中文刊名：BXYY 英文刊名：China Journal of Traditional Chinese Medicine and Pharmacy 机构：湖南中医药大学; 出版日期：2019-07-01 出版单位：中华中医药杂志 年：2019 期：v.34 基金：国家自然科学基金面上项目(No.71208301);; 湖南省研究生科研创新项目(No.CX2016B377,No.CX2017B432);; 湖南省教育厅双一流学科优秀青年基金项目(No.18B239);; 中医药防治五官科疾病湖南省重点实验室建设项目(No.2017TP1018);; 中央财政支持地方高校建设项目;; 湖南省高层次卫生人才“225”工程培养项目;; 国家中医药管理局中医眼科学重点学科建设项目;; 湖南省中医诊断学优势重点学科建设项目;; 湖南省中医五官科学重点学科建设项目~~ 语种：中文; 页：BXYY201907128 页数：5 CN：07 ISSN：11-5334/R 分类号：465-469

摘要

目的:探究血小板衍生因子(PDGF)促视网膜色素上皮细胞(RPE)细胞增殖的最佳浓度,并由此研究活血散结中药复方含药血浆对PDGF干预下兔RPE细胞增殖及胰岛素样生长因子-Ⅰ(IGF-Ⅰ)表达的影响。方法:成年健康家兔为对象,制备含药血浆。观察不同浓度PDGF对兔RPE细胞增殖的影响,选择最佳作用于兔RPE细胞的PDGF浓度。将实验分成空白对照组、正常血浆组、PDGF组、PDGF+AG1296组、PDGF+含药血浆组,观察活血散结中药复方含药血浆对PDGF干预下兔RPE细胞增殖的影响以及PDGF干预下兔RPE细胞IGF-ⅠmRNA和蛋白表达的影响。结果:选择10μg/L PDGF作为干预RPE细胞的最佳浓度。与PDGF组比较,PDGF+AG1296组、PDGF+含药血浆组细胞增殖显著抑制(P<0.01)。PDGF+AG1296组、PDGF+含药血浆组与PDGF组比较,IGF-ⅠmRNA和蛋白表达显著下调(P<0.01)。结论:活血散结中药复方含药血浆能抑制PDGF作用下兔RPE细胞的增殖。活血散结中药复方含药血浆作用于PDGF干预下所致PVR的细胞模型可抑制RPE细胞IGF-Ⅰ的表达。
        Objective: To explore the best concentration of PDGF to promote the proliferation of RPE cells. To study the effect of drug-containing plasma of Huoxue Sanjie Formula on rabbit RPE cell proliferation and IGF-Ⅰ expression under the PDGF intervention. Methods: Adult healthy rabbits were used to prepare drug-containing plasma. The effects of different concentrations of PDGF on the proliferation of rabbit RPE cells were observed. The PDGF concentration optimally acting on rabbit RPE cells was selected. The study would be divided into control group and normal plasma group, the plasma PDGF groups, PDGF+AG1296 group and PDGF+drug-containing plasma group. To observe effect drug-containing plasma of Huoxue Sanjie Formula on rabbit RPE cell proliferation and IGF-Ⅰ mRNA and protein expression. Results: 10μg/L PDGF was selected as the optimal concentration for intervening RPE cells. It had significant inhibition of cell proliferation in PDGF+AG1296 group and PDGF+drug-containing plasma group(P<0.01). Compared with PDGF groups, IGF-Ⅰ mRNA, IGF-Ⅰ protein expression in PDGF+AG1296 and PDGF+drug-containing plasma group significantly lowered, the difference had statistical significance(P<0.01). Conclusion:Drug-containing plasma of Huoxue Sanjie Formula can inhibit PDGF under the action of rabbit RPE cells proliferation and the expression of IGF-Ⅰ in RPE cells.

引文

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