美洲大蠊提取液对创面愈合中TGF-β1通路中Smad6、Smad9表达的影响及超微病理结构观察
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摘要
目的:探索美洲大蠊提取液作用大鼠难愈合创面的生长优势及可能调控机制。方法:将造模成功后的大鼠按随机数字表法分为空白组、模型组、实验组、对照组,应用免疫组化观察Smad6、Smad9的表达;应用HE染色观察细胞病理形态;应用透射电镜观察细胞超微结构。结果:第20天时,与模型组、空白组、对照组比较,实验组Smad6表达显著下降,Smad9表达显著上升(P<0.05)。病理形态显示,实验组细胞炎性渗出、水肿明显,炎性反应进程迅速;透射电镜显示,实验组较模型组、对照组细胞结构连接紧密,线粒体数量增加(P<0.05)。结论:在TGF-β1通路中,美洲大蠊提取液可能通过下调Smad6,上调Smad9表达,加速创面细胞病理炎性反应进程,促进细胞线粒体、内质网、桥粒等的增生与分化,促进创面愈合。
Objective: To explore the growth advantages and possible mechanism of on American cockroach extracting rat wounds with refractory healing. Methods: After successful modeling, rats were divided by random number table method into control group, model group, positive group and experimental group according to the balance. The expression of Smad6 and Smad9 was observed by immunohistochemistry. Cytopathological morphology was observed by HE staining. The ultrastructure of cells was observed by transmission electron microscopy. Results: The expression of Smad9 increased and the expression of Smad6 decreased in the experimental group, which was statistically significant compared with control group, model group,positive group on the 20 th day(P<0.05). Pathologically, the experimental group had obvious inflammatory exudation and edema in wound tissue, and the inflammatory reaction process was rapid, which was statistically significant compared with the other three groups. Compared with the model group and the control group, the experimental group had more closely connected and numerous cells, which had statistical significance(P<0.05). Conclusion: American cockroach extracting may effectively down-regulate the expression of Smad6 and up-regulate the expression of Smad9, accelerate the pathologic inflammatory response process of wound cells, promote the proliferation and differentiation of cell mitochondria, endoplasmic reticulum and desmosomes, and promote wound healing.
Objective: To explore the growth advantages and possible mechanism of on American cockroach extracting rat wounds with refractory healing. Methods: After successful modeling, rats were divided by random number table method into control group, model group, positive group and experimental group according to the balance. The expression of Smad6 and Smad9 was observed by immunohistochemistry. Cytopathological morphology was observed by HE staining. The ultrastructure of cells was observed by transmission electron microscopy. Results: The expression of Smad9 increased and the expression of Smad6 decreased in the experimental group, which was statistically significant compared with control group, model group,positive group on the 20 th day(P<0.05). Pathologically, the experimental group had obvious inflammatory exudation and edema in wound tissue, and the inflammatory reaction process was rapid, which was statistically significant compared with the other three groups. Compared with the model group and the control group, the experimental group had more closely connected and numerous cells, which had statistical significance(P<0.05). Conclusion: American cockroach extracting may effectively down-regulate the expression of Smad6 and up-regulate the expression of Smad9, accelerate the pathologic inflammatory response process of wound cells, promote the proliferation and differentiation of cell mitochondria, endoplasmic reticulum and desmosomes, and promote wound healing.
引文
[1]明·李时珍.本草纲目.北京:人民卫生出版社,2000:1010
[2]唐乾利,狄钾骐,李杰辉,等.MEBT/MEBO对大鼠慢性难愈性皮肤溃疡创面肉芽组织EGF、EGFR表达的影响.中国烧伤疮疡杂志,2012,6(24):470-480
[3]姜珍倩,吴宝玉,苏永华.中医药促进创面生长因子分泌的研究进展.中华中医药杂志,2015,30(2):485-487
[4]李文涛,段文旭,张扬,等.生肌膏对大鼠创面肉芽组织中MMP-2,MMP-9及bFGF表达的影响.中华中医药杂志,2018,33(6):2605-2609
[5]张勇,秦娜,于斌,等.TGF/Smads信号转导通路的研究进展.广西医科大学学报,2009,26(1):155-157
[6]洪乐鹏,邵帅,谭珍,等.Purmorphamine通过调控Smad6的表达发挥抗炎作用.中国临床神经病学,2016,24(3):260-266
[7]王和建,张莉,余道伦,等.BMP4诱导的Smad9在小鼠卵泡发育中的作用及其机制的初步研究.中国畜牧兽医,2018,45(10):2902-2909
[8]徐杰男,阙华发.补肾填精方促进大鼠慢性难愈合创面修复愈合的作用机制探讨.四川中医,2015,8(10):34-37
[9]Quiros P M,Langer T,Lopez-Otin C,et al.New roles for mitochondria)proteases in health,ageing and disease.Nat Rev Mol Cell Biol,2015,16(6):345-359
[10]曹海燕,翟玉龙,孙夏承,等.基于电镜和免疫组化的线粒体数目标志物评估肝癌的初步研究.现代生物医学进展,2016,16(14):2646-2650
[11]曾娟妮,刘筱,伍伟明,等.美洲大蠊提取液对大鼠难愈合创面VEGF表达影响的研究.中国民族民间医药,2016,25(19):64-66
[12]陈颖超.康复新液治疗消化性溃疡的研究进展.中国中医药现代远程教育,2017,15(9):141-143
[2]唐乾利,狄钾骐,李杰辉,等.MEBT/MEBO对大鼠慢性难愈性皮肤溃疡创面肉芽组织EGF、EGFR表达的影响.中国烧伤疮疡杂志,2012,6(24):470-480
[3]姜珍倩,吴宝玉,苏永华.中医药促进创面生长因子分泌的研究进展.中华中医药杂志,2015,30(2):485-487
[4]李文涛,段文旭,张扬,等.生肌膏对大鼠创面肉芽组织中MMP-2,MMP-9及bFGF表达的影响.中华中医药杂志,2018,33(6):2605-2609
[5]张勇,秦娜,于斌,等.TGF/Smads信号转导通路的研究进展.广西医科大学学报,2009,26(1):155-157
[6]洪乐鹏,邵帅,谭珍,等.Purmorphamine通过调控Smad6的表达发挥抗炎作用.中国临床神经病学,2016,24(3):260-266
[7]王和建,张莉,余道伦,等.BMP4诱导的Smad9在小鼠卵泡发育中的作用及其机制的初步研究.中国畜牧兽医,2018,45(10):2902-2909
[8]徐杰男,阙华发.补肾填精方促进大鼠慢性难愈合创面修复愈合的作用机制探讨.四川中医,2015,8(10):34-37
[9]Quiros P M,Langer T,Lopez-Otin C,et al.New roles for mitochondria)proteases in health,ageing and disease.Nat Rev Mol Cell Biol,2015,16(6):345-359
[10]曹海燕,翟玉龙,孙夏承,等.基于电镜和免疫组化的线粒体数目标志物评估肝癌的初步研究.现代生物医学进展,2016,16(14):2646-2650
[11]曾娟妮,刘筱,伍伟明,等.美洲大蠊提取液对大鼠难愈合创面VEGF表达影响的研究.中国民族民间医药,2016,25(19):64-66
[12]陈颖超.康复新液治疗消化性溃疡的研究进展.中国中医药现代远程教育,2017,15(9):141-143
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