对NOD/SCID和SCID小鼠皮下接种的黑色素瘤生长特性比较
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摘要
目的比较两种常用免疫缺陷小鼠皮下接种黑色素肿瘤细胞后肿瘤生长及小鼠生存情况。方法 7和17周龄的NOD/SCID及SCID小鼠左腋下接种小鼠黑色素瘤细胞,接种第8天开始隔1天测量肿瘤长径和短径,计算肿瘤体积,每天观察小鼠生存情况,记录生存时间,待1组小鼠完全死亡结束实验。结果 a.7周龄不同品系小鼠接种小鼠黑色素瘤后,NOD/SCID较SCID小鼠肿瘤生长速度更快、中位生存期更长;17周龄2种品系小鼠肿瘤生长情况无显著性差异。b.同品系NOD/SCID和SCID小鼠接种小鼠黑色素瘤后,小周龄较大周龄肿瘤生长速度更快,中位生存期更短。结论与7和17周龄SCID小鼠及17周龄NOD/SCID小鼠相比,7周龄NOD/SCID小鼠肿瘤生长情况更好,生存期较为适当,可能更适合作为黑色素瘤小鼠模型的实验动物。
Objective To comparatively analyze tumor growth characteristics in two commonly used immunodeficiency mice,non-obese diabetic/severe combined immunodeficiency(NOD/SCID)and severe combined immunodeficiency(SCID)mice.Methods The tumor size and the cumulative survival time were calculated,after 7-and 17-week-old NOD/SCID and SCID mice were subcutaneously injected with melanoma B16 cells under left armpit.On the 8 th day after the inoculation,the long diameter and short diameter of the tumor were measured every other day,the tumor volume was calculated.The survival of the mice was observed every day,and the survival time was recorded.And the experiment was finished when the group of the mice were all dead.Results(1)Compared with SCID aged at 7 weeks,the tumor grew faster in NOD/SCID mice at the same age,and the median survival time for tumor-bearing mice was significantly longer.There was no significant difference in tumor growth between 17-week-old NOD/SCID and SCID mice.(2)After NOD/SCID and SCID mice were inoculated with melanoma B16 cells,tumor grew faster and the median survival time was shorter in younger NOD/SCID and SCID mice.Conclusion Compared with SCID mice at 7 or 17 weeks age and NOD/SCID mice at 17 weeks age,NOD/SCID mice at 7 weeks age are more suitable for melanoma mouse model with characteristics of rapid tumor growth and more appropriate median survival time.
Objective To comparatively analyze tumor growth characteristics in two commonly used immunodeficiency mice,non-obese diabetic/severe combined immunodeficiency(NOD/SCID)and severe combined immunodeficiency(SCID)mice.Methods The tumor size and the cumulative survival time were calculated,after 7-and 17-week-old NOD/SCID and SCID mice were subcutaneously injected with melanoma B16 cells under left armpit.On the 8 th day after the inoculation,the long diameter and short diameter of the tumor were measured every other day,the tumor volume was calculated.The survival of the mice was observed every day,and the survival time was recorded.And the experiment was finished when the group of the mice were all dead.Results(1)Compared with SCID aged at 7 weeks,the tumor grew faster in NOD/SCID mice at the same age,and the median survival time for tumor-bearing mice was significantly longer.There was no significant difference in tumor growth between 17-week-old NOD/SCID and SCID mice.(2)After NOD/SCID and SCID mice were inoculated with melanoma B16 cells,tumor grew faster and the median survival time was shorter in younger NOD/SCID and SCID mice.Conclusion Compared with SCID mice at 7 or 17 weeks age and NOD/SCID mice at 17 weeks age,NOD/SCID mice at 7 weeks age are more suitable for melanoma mouse model with characteristics of rapid tumor growth and more appropriate median survival time.
引文
[1] CSCO黑色素瘤专家委员会.中国黑色素瘤诊治指南(2011版)[J].临床肿瘤学杂志,2012,17(2):159-171.
[2] 曾红梅,张思维,郑荣寿,等.2003～2007年中国皮肤黑色素瘤发病与死亡分析[J].中国肿瘤,2012,21(3),183-189.
[3] GRYGIELSKA B,KAMIENICZNA M,WILAND E,et al.In situ reconstruction of humoral immune response against sperm:comparison of SCID and NOD/SCID mouse models[J].American Journal of Reproductive Immunology,2009,61(2):147-157.
[4] VALENTINER U,HALL D M,BROOKS S A,et al.HPA binding and metastasis formation of human breast cancer cell lines transplanted into severe combined immunodeficient(scid)mice[J].Cancer Letters,2005,219(2):233-242.
[5] 杨文华,于文俊,史哲新,等.人白血病-NOD/SCID小鼠髓外浸润模型的建立与鉴定[J].临床与实验病理学杂志,2009,25(2):178-182.
[6] 于文俊,杨文华,史哲新,等.NOD/SCID小鼠模型在实验血液学研究中的应用[J].中国实验动物学报,2008,16(5):391-395.
[7] 徐珊,李增强,齐欢,等.7-乙基-10-羟基喜树碱脂质体的抗肿瘤作用[J].沈阳药科大学学报,2015,32(3):218-222.
[8] 杜丽媛,陈燕,宋荣霞,等.吠喃二烯抑制脑胶质瘤作用[J].沈阳药科大学学报,2013,30(4):292-297.
[9] 李翔,张婧,王东凯,等.叶酸受体靶向多烯紫杉醇膜修饰脂质体的抗肿瘤活性[J].药学学报,2013,48(7):1142-1147.
[10] 吴晓东,杨永朝,宋献美.人脐带间充质干细胞对NOD/SCID小鼠乳腺原位移植人乳腺癌MCF-7细胞增殖分化的影响[J].肿瘤基础与临床,2013,26(5):379-382.
[11] HIRAMATSU H,NISHIKOMORI R,HEIKE T,et al.Complete reconstitution of human lymphocytes from cord blood CD34+cells using the NOD/SCID/gamma null mice model[J].Blood,2003,102(3):873-880
[12] 孙冰,吴世凯,宋三泰.NOD/SCID小鼠平台上的肿瘤实验研究进展[J].中华肿瘤防治杂志,2008,15(4):307-310.
[13] SMYTH M J,HAYAKAWA Y,TAKEDA K,et al.New aspects of natural-killer-cell surveillance and therapy of cancer[J].Nature reviews,2002,2:850-861.
[14] 张盈华,张志培,赵佐庆.免疫缺陷鼠重建免疫后抑制移植瘤生长的研究[J].免疫学杂志,2002,18(6):431-433.
[15] 孙志方,黄强,衣服新,等.SCID小鼠的免疫学特征[J].上海实验动物科学,2000,20(3):159-161.
[16] 洑翔科,邓忠彬,朱伟,等.人多发性骨髓瘤荷瘤SCID鼠模型的建立及其生长特性[J].上海实验动物科学,2004,24(4):230-233.
[17] 陈步云,朱乃硕.严重联合免疫缺陷小鼠在肿瘤与免疫研究中的应用[J].河北医科大学学报,2005,26(4):316-320.
[18] 许文静,郭建光,吴艳秋,等.SPF级SCID.BG小鼠与BALB/c裸小鼠、SCID小鼠部分免疫指标的测定与比较[J].实验动物与比较医学,2013,33(2):153-156.
[19] 钱建新,周丽英,黄强,等.SCID 小鼠外周血临检指标的检测[J].苏州医学院学报,2000,20(3):215-217.
[2] 曾红梅,张思维,郑荣寿,等.2003～2007年中国皮肤黑色素瘤发病与死亡分析[J].中国肿瘤,2012,21(3),183-189.
[3] GRYGIELSKA B,KAMIENICZNA M,WILAND E,et al.In situ reconstruction of humoral immune response against sperm:comparison of SCID and NOD/SCID mouse models[J].American Journal of Reproductive Immunology,2009,61(2):147-157.
[4] VALENTINER U,HALL D M,BROOKS S A,et al.HPA binding and metastasis formation of human breast cancer cell lines transplanted into severe combined immunodeficient(scid)mice[J].Cancer Letters,2005,219(2):233-242.
[5] 杨文华,于文俊,史哲新,等.人白血病-NOD/SCID小鼠髓外浸润模型的建立与鉴定[J].临床与实验病理学杂志,2009,25(2):178-182.
[6] 于文俊,杨文华,史哲新,等.NOD/SCID小鼠模型在实验血液学研究中的应用[J].中国实验动物学报,2008,16(5):391-395.
[7] 徐珊,李增强,齐欢,等.7-乙基-10-羟基喜树碱脂质体的抗肿瘤作用[J].沈阳药科大学学报,2015,32(3):218-222.
[8] 杜丽媛,陈燕,宋荣霞,等.吠喃二烯抑制脑胶质瘤作用[J].沈阳药科大学学报,2013,30(4):292-297.
[9] 李翔,张婧,王东凯,等.叶酸受体靶向多烯紫杉醇膜修饰脂质体的抗肿瘤活性[J].药学学报,2013,48(7):1142-1147.
[10] 吴晓东,杨永朝,宋献美.人脐带间充质干细胞对NOD/SCID小鼠乳腺原位移植人乳腺癌MCF-7细胞增殖分化的影响[J].肿瘤基础与临床,2013,26(5):379-382.
[11] HIRAMATSU H,NISHIKOMORI R,HEIKE T,et al.Complete reconstitution of human lymphocytes from cord blood CD34+cells using the NOD/SCID/gamma null mice model[J].Blood,2003,102(3):873-880
[12] 孙冰,吴世凯,宋三泰.NOD/SCID小鼠平台上的肿瘤实验研究进展[J].中华肿瘤防治杂志,2008,15(4):307-310.
[13] SMYTH M J,HAYAKAWA Y,TAKEDA K,et al.New aspects of natural-killer-cell surveillance and therapy of cancer[J].Nature reviews,2002,2:850-861.
[14] 张盈华,张志培,赵佐庆.免疫缺陷鼠重建免疫后抑制移植瘤生长的研究[J].免疫学杂志,2002,18(6):431-433.
[15] 孙志方,黄强,衣服新,等.SCID小鼠的免疫学特征[J].上海实验动物科学,2000,20(3):159-161.
[16] 洑翔科,邓忠彬,朱伟,等.人多发性骨髓瘤荷瘤SCID鼠模型的建立及其生长特性[J].上海实验动物科学,2004,24(4):230-233.
[17] 陈步云,朱乃硕.严重联合免疫缺陷小鼠在肿瘤与免疫研究中的应用[J].河北医科大学学报,2005,26(4):316-320.
[18] 许文静,郭建光,吴艳秋,等.SPF级SCID.BG小鼠与BALB/c裸小鼠、SCID小鼠部分免疫指标的测定与比较[J].实验动物与比较医学,2013,33(2):153-156.
[19] 钱建新,周丽英,黄强,等.SCID 小鼠外周血临检指标的检测[J].苏州医学院学报,2000,20(3):215-217.
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