辅助性Th17细胞在HPV感染宫颈病变患者外周血的比例及临床意义
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摘要
目的探讨辅助性Th17细胞在人乳头状瘤病毒(HPV)感染宫颈病变患者外周血的比例及作用机制。方法将2016年1月-2018年1月间在医院就诊的151例宫颈病变HPV感染患者根据病变类型分为慢性宫颈炎(CCS)组42例、宫颈上皮内瘤变(CIN)组51例、宫颈癌(CC)组58例。检测三组HPV分型,比较不同宫颈病变类型患者以及不同HPV感染类型患者外周血辅助性Th17细胞比例及白介素-17(IL-17)、白介素-23(IL-23)水平,并比较不同临床特征的CC患者外周血Th17细胞比例。结果 CCS组、CIN组和CC组Th17细胞比例、IL-17、IL-23水平以及高危型HPV感染发生率均依次升高(P<0.05)。高危型HPV感染患者外周血Th17细胞比例及IL-17、IL-23水平均明显高于低危型HPV感染患者(P<0.05)。CC患者外周血Th17细胞比例与FIGO分期和分化程度有关(P<0.05)。存在脉管浸润、淋巴结转移的CC患者外周血Th17细胞比例均明显高于无脉管浸润、无淋巴结转移的CC患者(P<0.05)。结论随着宫颈病变程度的恶化,外周血辅助性Th17细胞比例升高。Th17细胞可能参与高危型HPV感染引导的免疫逃逸过程而促进CC的发生、发展。
OBJECTIVE To explore the percentage of helper Th17 cells in peripheral blood of patients with cervical lesions human papillomavirus(HPV) and observe its action mechanisms. METHODS A total of 151 patients with cervical lesions HPV infection who were treated in the hospital from Jan 2016 to Jan 2018 were enrolled in the study and divided into the chronic cervicitis(CCS) group with 42 cases, the cervical intraepithelial neoplasia(CIN) group with 51 cases and the cervical cancer(CC)group with 58 cases according to the types of lesions. The types of HPV were detected, the percentage of peripheral blood helper Th 17 cells and levels of interleukin-17(IL-17) and interleukin-23(IL-23) were compared among the three groups of patients and among the patients with different types of HPV infection, and the percentage of peripheral blood helper Th17 cells among the patients with CC. RESULTS The percentage of Th17 cells and levels of IL-17 and IL-23 as well as the incidence of high-risk HPV infection were successively elevated in the CCS group, the CIN group and the CC group(P<0.05); the percentage of peripheral blood Th17 cells and levels of IL-17 and IL-23 of the patients with high-risk HPV infection were significantly lower than those of the patients with low-risk HPV infection(P<0.05). The percentage of peripheral blood Th17 cells of the CC patients was associated with the FIGO stage and differentiation degree(P<0.05). The percentage of peripheral blood Th17 cells of the CC patients who had vascular infiltration and lymph node metastasis was significantly higher than that of the CC patients who did not have vascular infiltration and lymph node metastasis( P<0.05). CONCLUSION With the deterioration of the degree of cervical lesions, the percentage of peripheral blood helper Th cells is elevated. Th17 cells may participate in the process of immune escape induced by high risk HPV infection and promote the occurrence and development of CC.
OBJECTIVE To explore the percentage of helper Th17 cells in peripheral blood of patients with cervical lesions human papillomavirus(HPV) and observe its action mechanisms. METHODS A total of 151 patients with cervical lesions HPV infection who were treated in the hospital from Jan 2016 to Jan 2018 were enrolled in the study and divided into the chronic cervicitis(CCS) group with 42 cases, the cervical intraepithelial neoplasia(CIN) group with 51 cases and the cervical cancer(CC)group with 58 cases according to the types of lesions. The types of HPV were detected, the percentage of peripheral blood helper Th 17 cells and levels of interleukin-17(IL-17) and interleukin-23(IL-23) were compared among the three groups of patients and among the patients with different types of HPV infection, and the percentage of peripheral blood helper Th17 cells among the patients with CC. RESULTS The percentage of Th17 cells and levels of IL-17 and IL-23 as well as the incidence of high-risk HPV infection were successively elevated in the CCS group, the CIN group and the CC group(P<0.05); the percentage of peripheral blood Th17 cells and levels of IL-17 and IL-23 of the patients with high-risk HPV infection were significantly lower than those of the patients with low-risk HPV infection(P<0.05). The percentage of peripheral blood Th17 cells of the CC patients was associated with the FIGO stage and differentiation degree(P<0.05). The percentage of peripheral blood Th17 cells of the CC patients who had vascular infiltration and lymph node metastasis was significantly higher than that of the CC patients who did not have vascular infiltration and lymph node metastasis( P<0.05). CONCLUSION With the deterioration of the degree of cervical lesions, the percentage of peripheral blood helper Th cells is elevated. Th17 cells may participate in the process of immune escape induced by high risk HPV infection and promote the occurrence and development of CC.
引文
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[14] Mahmoud Hashemi A,Solahaye Kahnamouii S,Aghajani H,et al.Quercetin decreases Th17 production by down-regulation of MAPK-TLR4 signaling pathway on T cells in dental pulpitis[J].J Dent (Shiraz),2018,19(4):259-264.
[15] Yang CH,Tian JJ,Ko WS,et al.Oligo-fucoidan improved unbalance the Th1/Th2 and Treg/Th17 ratios in asthmatic patients:An ex vivo study[J].Exp Ther Med,2019,17(1):3-10.
[16] Zhang Q,Liu S,Ge D,et al.Targeting Th17-IL-17 pathway in prevention of micro-invasive prostate cancer in a mouse model[J].Prostate,2017,77(8):888-899.
[17] Mao H,Pan F,Guo H,et al.Feedback mechanisms between M2 macrophages and Th17 cells in colorectal cancer patients[J].Tumour Biol,2016,37(9):12223-12230.
[18] 李燕波,何志良,苏丹妮,等.舌鳞癌患者外周血Th17细胞及其细胞因子变化的临床意义[J].重庆医学,2017,46(20):2761-2763.
[19] Wang LX,Kang ZP,Yang ZC,et al.MicroRNA-135a inhibits nasopharyngeal carcinoma cell proliferation through targeting interleukin-17[J].Cell Physiol Biochem,2018,46(6):2232-2238.
[20] 林晨红,管剑龙.干扰素调节因子8抑制辅助性T淋巴细胞17分化在白塞病发病机制中的作用[J].第二军医大学学报,2018,39(5):535-537.
[21] Lee MH,Tung-Chieh Chang J,Liao CT,et al.Interleukin 17 and peripheral IL-17-expressing T cells are negatively correlated with the overall survival of head and neck cancer patients[J].Oncotarget,2018,9(11):9825-9837.
[22] 蔡冬慧,李燕,郭润梅,等.宫颈癌HPV分型与Th17和Treg细胞的相关性研究[J].中国肿瘤临床,2016,43(24):1099-1102.
[23] 陶陶,高嵩.CIN及宫颈癌患者外周Treg/Th17细胞的表达及意义[J].中国妇幼健康研究,2018,29(2):174-177.
[24] 陈志芳,蔡冬慧,范玲玲,等.维吾尔族宫颈癌患者手术前后HPV感染与Th 17/Treg细胞的相关性[J].新疆医科大学学报,2017,40(1):106-110.
[25] 刘佳,蔡小凤.白细胞介素-17基因启动子区域甲基化与宫颈癌的关系研究[J].中国计划生育和妇产科,2017,9(12):64-66.
[26] 潘铭,黄建霞,王宁,等.探索Th17细胞在急性髓细胞白血病中的作用[J].临床血液学杂志,2016,29(3):389-391.
[2] 孙娟娟,宋晓婕,罗自娟,等.高危型HPV筛查与TCT联合检查在宫颈癌筛查中的临床应用[J].现代生物医学进展,2016,16(12):2336-2338.
[3] Bruggmann D,Kayser L,Jaque J,et al.Human papilloma virus:global research architecture assessed by density-equalizing mapping[J].Oncotarget,2018,9(31):21965-21977.
[4] Seneldir H,Kir G.Prevalence of high-risk human papilloma virus in liquid-based cervical samples from Turkish women with normal and abnormal cytology[J].Diagn Cytopathol,2019,47(2):100-104.
[5] 陈海涛,兰建云,翁国武,等.宫颈上皮内瘤变组织中HPV型别分布情况分析[J].国际检验医学杂志,2018,39(22):2778-2781.
[6] Sun LX,Wang XB,Huang XJ.Association analysis of rs2275913G>A and rs763780T>C interleukin 17 polymorphisms in Chinese women with cervical cancer[J].Genet Mol Res,2015,14(4):13612-13617.
[7] 丁琦,黄建安.Th17/IL-17在急性肺损伤中的研究进展[J].临床肺科杂志,2018,23(11):2111-2113.
[8] 胡萍,王芳,周星.Th1/Th2、Treg/Th17细胞平衡在慢性乙型肝炎发病机制中的作用[J].实用医学杂志,2018,34(18):3070-3072.
[9] 林仲秋.FIGO/IGCS妇科恶性肿瘤分期及临床实践指南之三:宫颈癌[J].国际妇产科学杂志,2008,35(3):226-228.
[10] 陈乐真.妇产科诊断病理学[M].北京:人民军医出版社,2002:116-136.
[11] 吴翠霞,张艳红,葛小花,等.高危型HPV感染与宫颈癌前病变与宫颈癌的相关性研究[J].中华医院感染学杂志,2016,26(11):2568-2570.
[12] Valls-Ontanon A,Hernandez-Losa J,Somoza Lopez de Haro R,et al.Impact of human papilloma virus in patients with oral and oropharyngeal squamous cell carcinomas[J].Med Clin (Barc),2019,152(5):174-180.
[13] 刘娇,王川林,古雪,等.Th17/Treg细胞失衡与乙肝病毒感染相关性肝病的关系[J].川北医学院学报,2017,32(3):479-482.
[14] Mahmoud Hashemi A,Solahaye Kahnamouii S,Aghajani H,et al.Quercetin decreases Th17 production by down-regulation of MAPK-TLR4 signaling pathway on T cells in dental pulpitis[J].J Dent (Shiraz),2018,19(4):259-264.
[15] Yang CH,Tian JJ,Ko WS,et al.Oligo-fucoidan improved unbalance the Th1/Th2 and Treg/Th17 ratios in asthmatic patients:An ex vivo study[J].Exp Ther Med,2019,17(1):3-10.
[16] Zhang Q,Liu S,Ge D,et al.Targeting Th17-IL-17 pathway in prevention of micro-invasive prostate cancer in a mouse model[J].Prostate,2017,77(8):888-899.
[17] Mao H,Pan F,Guo H,et al.Feedback mechanisms between M2 macrophages and Th17 cells in colorectal cancer patients[J].Tumour Biol,2016,37(9):12223-12230.
[18] 李燕波,何志良,苏丹妮,等.舌鳞癌患者外周血Th17细胞及其细胞因子变化的临床意义[J].重庆医学,2017,46(20):2761-2763.
[19] Wang LX,Kang ZP,Yang ZC,et al.MicroRNA-135a inhibits nasopharyngeal carcinoma cell proliferation through targeting interleukin-17[J].Cell Physiol Biochem,2018,46(6):2232-2238.
[20] 林晨红,管剑龙.干扰素调节因子8抑制辅助性T淋巴细胞17分化在白塞病发病机制中的作用[J].第二军医大学学报,2018,39(5):535-537.
[21] Lee MH,Tung-Chieh Chang J,Liao CT,et al.Interleukin 17 and peripheral IL-17-expressing T cells are negatively correlated with the overall survival of head and neck cancer patients[J].Oncotarget,2018,9(11):9825-9837.
[22] 蔡冬慧,李燕,郭润梅,等.宫颈癌HPV分型与Th17和Treg细胞的相关性研究[J].中国肿瘤临床,2016,43(24):1099-1102.
[23] 陶陶,高嵩.CIN及宫颈癌患者外周Treg/Th17细胞的表达及意义[J].中国妇幼健康研究,2018,29(2):174-177.
[24] 陈志芳,蔡冬慧,范玲玲,等.维吾尔族宫颈癌患者手术前后HPV感染与Th 17/Treg细胞的相关性[J].新疆医科大学学报,2017,40(1):106-110.
[25] 刘佳,蔡小凤.白细胞介素-17基因启动子区域甲基化与宫颈癌的关系研究[J].中国计划生育和妇产科,2017,9(12):64-66.
[26] 潘铭,黄建霞,王宁,等.探索Th17细胞在急性髓细胞白血病中的作用[J].临床血液学杂志,2016,29(3):389-391.
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