红果小檗果乙醇提取物对四氯化碳致急性肝损伤小鼠的保护作用
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摘要
目的评价红果小檗果乙醇提取物对四氯化碳(CCl_4)所致小鼠急性肝损伤模型的保肝作用。方法将60只小鼠随机分成模型组(生理盐水10 mL/kg体质量),阳性对照组(联苯双酯5 mg/kg体质量),红果小檗乙醇提取物低、中、高剂量组(0.375、 0.75、1.50 g/kg体质量),另取12只小鼠作为正常对照组(生理盐水10 mL/kg体质量),每天灌胃1次,连续7 d。末次给药2 h后,除正常对照组外其他各组小鼠腹腔注射0.1%四氯化碳花生油溶液(10 mL/kg体质量),收集各组小鼠血液和肝脏,采用全自动生化分析仪测定小鼠血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)含量,测定小鼠肝组织中丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)含量和超氧化物歧化酶(SOD)的含量。显微观察各组小鼠肝组织病理学改变情况。结果与正常对照组比较,模型组小鼠血清ALT、AST含量均升高,差异有统计学意义(P<0.05);与模型组小鼠比较,红果小檗果乙醇提取物低、中、高剂量组小鼠血清ALT、AST含量降低,差异有统计学意义(P<0.05)。与正常对照组比较,模型组小鼠肝组织SOD、GSH-PX含量降低,MDA含量升高,差异有统计学意义(P<0.05);与模型组比较,阳性对照组、红果小檗乙醇提取物低、中、高剂量组小鼠肝组织SOD、GSH-PX含量升高,MDA含量降低,差异有统计学意义(P<0.05)。显微观察显示正常组小鼠肝小叶结构正常,肝细胞无坏死、变性和炎性浸润等现象;模型组肉眼可见小鼠肝脏肿大、充血、质地脆弱、肝小叶界限模糊、肝索排列紊乱,肝细胞变性,被大量炎性细胞浸润;红果小檗乙醇提取物低、中、高剂量组小鼠肝小叶结构恢复正常,肝细胞形态正常,炎性细胞浸润减少。结论红果小檗果乙醇提取物对CCl_4致小鼠急性化学性肝损伤具有一定的保护作用。
Objective To evaluate the hepatoprotective effect of ethanol extract of Berberisheteropoda on acute liver injury model induced by carbon tetrachloride(CCl_4) in mice. Methods Sixty mice were randomly divided into model group(10 mL/kg body mass of normal saline), positive control group(5 mg/kg body mass of biphenylenediester), low, medium and high dose group(0.375, 0.75, 1.50 g/kg body mass of alcohol extract of Berberisheteropoda). Another 12 mice were taken as normal control group(10 mL/kg body mass of normal saline) and gavaged once a day for 7 days. Two hours after the last administration, the mice in each group were injected with 0.1% carbon tetrachloride peanut oil solution(10 mL/kg body mass) intraperitoneally, and the blood and liver of each group were collected. The levels of alanine aminotransferase(ALT) and glutathione aminotransferase(AST) in the serum of mice were determined by automatic biochemical analyzer. The contents of Malondialdehyde(MDA), glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) in the liver tissue of mice were measured. The pathological changes of liver tissue were observed by microscopy. Results Compared with the normal control group, the levels of serum ALT and AST in the model group increased significantly(P<0.05). Compared with the model group, the levels of serum ALT and AST in the low, medium and high dose groups of alcohol extract from Berberisheteropoda decreased significantly(P<0.05). Compared with the normal control group, the contents of SOD and GSH-Px of liver in the model group decreased, while the content of MDA increased(P<0.05); compared with the model group, the contents of SOD and GSH-Px of liver in the positive control group, the ethanol extract of Berberisheteropoda low, medium and high dose groups increased, and the content of MDA decreased(P<0.05). Microscopic observation showed that the structure of hepatic lobules in the normal mice was normal, and there were no necrosis, degeneration and inflammatory infiltration of hepatocytes. The naked eyes of the model group showed that the hepatic lobules of mice were enlarged, congested and fragile, the boundary of hepatic lobules was blurred, the hepatic cord was arranged disorderly, and the hepatocytes were degenerated and infiltrated by a large number of inflammatory cells. In low, medium and high dose groups of Berberis, the structure of hepatic lobules of mice returned to normal, the morphology of hepatocyte was normal, and inflammatory cell infiltration reduced. Conclusion Ethanol extract from Berberisheteropoda capsules have a certain liver-protecting effect on acute chemical liver injury induced by CCl_4 in mice.
Objective To evaluate the hepatoprotective effect of ethanol extract of Berberisheteropoda on acute liver injury model induced by carbon tetrachloride(CCl_4) in mice. Methods Sixty mice were randomly divided into model group(10 mL/kg body mass of normal saline), positive control group(5 mg/kg body mass of biphenylenediester), low, medium and high dose group(0.375, 0.75, 1.50 g/kg body mass of alcohol extract of Berberisheteropoda). Another 12 mice were taken as normal control group(10 mL/kg body mass of normal saline) and gavaged once a day for 7 days. Two hours after the last administration, the mice in each group were injected with 0.1% carbon tetrachloride peanut oil solution(10 mL/kg body mass) intraperitoneally, and the blood and liver of each group were collected. The levels of alanine aminotransferase(ALT) and glutathione aminotransferase(AST) in the serum of mice were determined by automatic biochemical analyzer. The contents of Malondialdehyde(MDA), glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) in the liver tissue of mice were measured. The pathological changes of liver tissue were observed by microscopy. Results Compared with the normal control group, the levels of serum ALT and AST in the model group increased significantly(P<0.05). Compared with the model group, the levels of serum ALT and AST in the low, medium and high dose groups of alcohol extract from Berberisheteropoda decreased significantly(P<0.05). Compared with the normal control group, the contents of SOD and GSH-Px of liver in the model group decreased, while the content of MDA increased(P<0.05); compared with the model group, the contents of SOD and GSH-Px of liver in the positive control group, the ethanol extract of Berberisheteropoda low, medium and high dose groups increased, and the content of MDA decreased(P<0.05). Microscopic observation showed that the structure of hepatic lobules in the normal mice was normal, and there were no necrosis, degeneration and inflammatory infiltration of hepatocytes. The naked eyes of the model group showed that the hepatic lobules of mice were enlarged, congested and fragile, the boundary of hepatic lobules was blurred, the hepatic cord was arranged disorderly, and the hepatocytes were degenerated and infiltrated by a large number of inflammatory cells. In low, medium and high dose groups of Berberis, the structure of hepatic lobules of mice returned to normal, the morphology of hepatocyte was normal, and inflammatory cell infiltration reduced. Conclusion Ethanol extract from Berberisheteropoda capsules have a certain liver-protecting effect on acute chemical liver injury induced by CCl_4 in mice.
引文
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[13] 易婷婷,罗光成,胡帅,等.血浆谷氨酸脱氢酶活性测定在肝胆疾病中的临床应用评价[J].川北医学院学报,2016,31(4):532-535.
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[16] 许金鹏,张慧慧,李朝品,等.原卟啉钠对四氯化碳致小鼠急性肝损伤的保护作用及其机制[J].中国实验方剂学杂志,2011,17(5):168-172.
[2] 孙华.小檗色素的提取及其理化性质研究[J].生物技术通讯,2005,16(4):405-406.
[3] 库尔班江·亥依如拉木,罗新泽.伊犁河流域红果小檗资源分布与形态调查[J].中国野生植物资源,2007,26(5):26-30.
[4] 邓贵华,林朝展,祝晨,等.穿心莲药材及其制剂中6个内酯类成分的含量分析[J].药物分析杂志,2011,30(2):231-235.
[5] 林朝展,邓贵华,祝晨.HPLC同时测定穿心莲药材及其制剂中的6种内酯类成分[J].华西药学杂志,2011,26(1):67-70.
[6] 任睿,张园园.反相高效液相色谱法测定喉舒宁片中穿心莲内酯和脱水穿心莲内酯的含量[J].中南药学,2011,9(4):271-273.
[7] 徐冲,王峥涛.穿心莲根的化学成分研究[J].药学学报,2011,46(3):317-321.
[8] 蒋珍藕.穿心莲属植物化学成分研究进展[J].中成药,2011,33(8):1382-1387.
[9] 田莉,杨建,田树革,等.小檗果提取物的体外抑菌实验研究[J].食品工业科技,2012,34(16):148-150.
[10] 陈奇.中药药理研究方法学[M].北京:人民卫生出版社,1993:78-79.
[11] 李舒丹,厉有名,虞朝辉,等.大鼠慢性酒精性肝损伤模型的建立[J].浙江医学2002,24(9):524-525.
[12] 刘城移,戚梦,吕虹燕,等.黑木耳黑色素对急性肝损伤的改善作用[J].菌物学报,2018,37(12):1741-1750.
[13] 易婷婷,罗光成,胡帅,等.血浆谷氨酸脱氢酶活性测定在肝胆疾病中的临床应用评价[J].川北医学院学报,2016,31(4):532-535.
[14] OZDEN S,CATALGOL B,GEZGINCI-OKTAYOGLU S,et a1.Methiocarb-induced oxidative damage following subacute exposure and the protective effects of vitamin E and taurine in rats[J].Food Chem Toxicol,2009,47(7):1676-1684.
[15] 阿依西姑·巴伍墩,阿迪力·阿不都热合曼,赵双清,等.黑果小檗提取物对高脂血症大鼠血脂/血液流变学和脂质过氧化水平的影响[J].新疆医科大学学报,2018,41(2):211-216.
[16] 许金鹏,张慧慧,李朝品,等.原卟啉钠对四氯化碳致小鼠急性肝损伤的保护作用及其机制[J].中国实验方剂学杂志,2011,17(5):168-172.
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