水产动物Akt基因研究进展
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摘要
Akt(RAC-alpha serine/threonine-protein kinase)是一种丝氨酸(Ser)或苏氨酸(Thr)蛋白激酶,也称为蛋白激酶B(PKB),可以磷酸化多种转录因子,在细胞增殖、细胞免疫应答、细胞代谢调控、细胞发育及存活等过程中发挥重要作用。目前有关水产动物Akt基因及其生物学功能的研究处于起步阶段,资料尚缺乏,本研究中就近年来水产动物Akt基因的序列特征、进化特点、参与的主要通路,以及参与调控鱼类的胚胎发育过程、甲壳类与贝类的免疫防御过程等生物功能等进行综述,指出今后可利用同源克隆或基因组数据挖掘技术获得更多水产动物的Akt基因信息,以便系统和全面地了解该基因在水产动物中的系统进化特点和规律,深入探究不同生理、病理条件下,不同水产动物的Akt基因的响应规律及该基因与其下游靶基因的互作机制,进一步筛选和挖掘能够调控水产动物Akt基因表达的表观遗传调控因子。本研究结果可为充分认识和利用水产动物Akt基因的生物学功能提供更为丰富的资料和线索。
Akt(RAC-alpha serine/threonine-protein kinase)as a serine(Ser) or threonine(Thr) protein kinase, known as protein kinase B(PKB, functions as phosphorylation of a variety of transcription factors, and plays an essential rolein cell multiplication, cellular immune response, cell metabolism regulation, cell development and survival. At present, there were few research on Akt gene in aquatic animals, so we reviewed the sequence characteristics, evolutionary characteristics, and main pathways involved in Akt gene in aquatic animals, as well as the biological functions involved in regulation of embryonic development of fish, and immune defense processes of crustaceans and shellfish. In the future, more Akt gene information of aquatic animals can be obtained by homologous cloning or genome data mining technology so as to understand systematically and comprehensively the evolution characteristics and rules of Akt gene in aquatic animals, and to further explore the response rules of Akt gene in different aquatic animals and the interaction mechanism between Akt gene and downstream target genes under different physiological and pathological conditions. Further screening and mining of epigenetic regulators that can regulate Akt gene expression in aquatic animals can provide abundant information and clues for fully understanding and utilizing of the biological functions of Akt gene in aquatic animals.
Akt(RAC-alpha serine/threonine-protein kinase)as a serine(Ser) or threonine(Thr) protein kinase, known as protein kinase B(PKB, functions as phosphorylation of a variety of transcription factors, and plays an essential rolein cell multiplication, cellular immune response, cell metabolism regulation, cell development and survival. At present, there were few research on Akt gene in aquatic animals, so we reviewed the sequence characteristics, evolutionary characteristics, and main pathways involved in Akt gene in aquatic animals, as well as the biological functions involved in regulation of embryonic development of fish, and immune defense processes of crustaceans and shellfish. In the future, more Akt gene information of aquatic animals can be obtained by homologous cloning or genome data mining technology so as to understand systematically and comprehensively the evolution characteristics and rules of Akt gene in aquatic animals, and to further explore the response rules of Akt gene in different aquatic animals and the interaction mechanism between Akt gene and downstream target genes under different physiological and pathological conditions. Further screening and mining of epigenetic regulators that can regulate Akt gene expression in aquatic animals can provide abundant information and clues for fully understanding and utilizing of the biological functions of Akt gene in aquatic animals.
引文
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[8] 王富轩.香港牡蛎(Crassostrea hongkongensis)Akt-FoxO信号通路相关基因的分子克隆与功能鉴定[D].北京:中国科学院大学,2016.
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[13] Vasudevan K M,Garraway L A.AKT signaling in physiology and disease[M]//Rommel C,Vanhaesebroeck B,Vogt P K.Phosphoinositide 3-Kinase in Health and Disease.Berlin,Heidelberg:Springer,2010:105-133.
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[15] Leslie N R,Downes C P.PTEN:the down side of PI 3-kinase signalling[J].Cellular Signalling,2002,14(4):285-295.
[16] 张海燕,孙红.Akt基因在上皮性卵巢癌组织中的表达及临床意义[J].现代妇产科进展,2014,23(12):964-967.
[17] 谢婧雯.Akt信号转导通路在心血管系统中作用的研究进展[J].重庆医学,2013,42(30):3692-3694.
[18] 晏淼,朱正秋.Akt/mTOR信号通路与恶性肿瘤[J].白求恩军医学院学报,2010,8(6):434-436.
[19] 杨运华.胰岛素抵抗对肝胰岛素PI3K/Akt/GSK-3信号传导通路的影响[J].黔南民族医专学报,2013,26(2):79-81,87.
[20] 迟毓婧,李晶,管又飞,等.PI3K-Akt信号传导通路对糖代谢的调控作用[J].中国生物化学与分子生物学报,2010,26(10):879-885.
[21] Lai K M V,Gonzalez M,Poueymirou W T,et al.Conditional activation of Akt in adult skeletal muscle induces rapid hypertrophy[J].Molecular and Cellular Biology,2004,24(21):9295-9304.
[22] 钟宇,王亚军,李娟.猪Akt1、Akt2基因克隆与组织表达图谱分析[J].四川大学学报:自然科学版,2014,51(2):365-370.
[23] 尚胜男,李开全,柳林,等.棘皮动物结合素研究进展[J].大连海洋大学学报,2018,33(1):113-122.
[24] Lawlor M A,Alessi D R.PKB/Akt:a key mediator of cell proliferation,survival and insulin responses?[J].Journal of Cell Science,2001,114:2903-2910.
[25] 胡明英,王晓莉,毕学辉,等.RNA干扰沉默AKT基因对卵巢癌SKOV3细胞增殖与凋亡影响研究[J].中华肿瘤防治杂志,2013,20(24):1869-1873.
[26] Liang Jiyong,Slingerland J M.Multiple roles of the PI3K/PKB (Akt) pathway in cell cycle progression[J].Cell Cycle,2003,2(4):339-345.
[27] 陈一飞.中国卤虫胚胎滞育解除过程中akt/pkb基因的作用及表达模式[D].大连:辽宁师范大学,2013.
[28] 王金凤,胡鹏,牛虹博,等.低温胁迫对鱼类PI3K/AKT/GSK-3β信号通路的影响[J].生物学杂志,2016,33(6):24-28.
[29] 田志环,焦传珍,成永旭,等.中华绒螯蟹Akt基因的cDNA克隆、序列分析及表达特征[J].水产学报,2018,42(4):485-494.
[30] 于鸿燕.草鱼三个PI3K/AKT通路相关基因的克隆和表达分析[D].上海:上海海洋大学,2015.
[2] Jones P F,Jakubowicz T,Pitossi F J,et al.Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily[J].Proceedings of the National Academy of Sciences of the United States of America,1991,88(10):4171-4175.
[3] 周颖,王建,贺福初.蛋白激酶B(PKB/Akt)的结构、调控与功能[J].生命的化学,2006,26(3):226-228.
[4] 王维.P13K/Akt信号转导通路的研究进展[J].现代医药卫生,2010,26(7):1051-1052.
[5] Chan J,Bayliss P E,Wood J M,et al.Dissection of angiogenic signaling in zebrafish using a chemical genetic approach[J].Cancer Cell,2002,1(3):257-267.
[6] 谌爽.RKB/Akt在斑马鱼早期胚胎发育中的功能研究与分析[D].青岛:中国海洋大学,2010.
[7] 迟艳红,李诗豪,王冬冬,等.中国明对虾(Fenneropenaeus chinensis)Akt/PKB基因在病原刺激下的表达和功能分析[J].海洋与湖沼,2013,44(3):611-617.
[8] 王富轩.香港牡蛎(Crassostrea hongkongensis)Akt-FoxO信号通路相关基因的分子克隆与功能鉴定[D].北京:中国科学院大学,2016.
[9] Bottomley M J,Salim K,Panayotou G.Phospholipid-binding protein domains[J].Biochimica et Biophysica Acta,1998,1436(1-2):165-183.
[10] 刘荣雕.凡纳滨对虾免疫相关分子LVAKT与LVFLOT的初步研究[D].厦门:国家海洋局第三海洋研究所,2013.
[11] 徐丹丹,唐彦君,王桂华,等.Akt基因的研究进展[J].生物技术世界,2014(1):8.
[12] Dunn E F,Connor J H.HijAkt:the PI3K/Akt pathway in virus replication and pathogenesis[J].Progress in Molecular Biology and Translational Science,2012,106:223-250.
[13] Vasudevan K M,Garraway L A.AKT signaling in physiology and disease[M]//Rommel C,Vanhaesebroeck B,Vogt P K.Phosphoinositide 3-Kinase in Health and Disease.Berlin,Heidelberg:Springer,2010:105-133.
[14] Maehama T,Dixon J E.PTEN:a tumour suppressor that functions as a phospholipid phosphatase[J].Trends in Cell Biology,1999,9(4):125-128.
[15] Leslie N R,Downes C P.PTEN:the down side of PI 3-kinase signalling[J].Cellular Signalling,2002,14(4):285-295.
[16] 张海燕,孙红.Akt基因在上皮性卵巢癌组织中的表达及临床意义[J].现代妇产科进展,2014,23(12):964-967.
[17] 谢婧雯.Akt信号转导通路在心血管系统中作用的研究进展[J].重庆医学,2013,42(30):3692-3694.
[18] 晏淼,朱正秋.Akt/mTOR信号通路与恶性肿瘤[J].白求恩军医学院学报,2010,8(6):434-436.
[19] 杨运华.胰岛素抵抗对肝胰岛素PI3K/Akt/GSK-3信号传导通路的影响[J].黔南民族医专学报,2013,26(2):79-81,87.
[20] 迟毓婧,李晶,管又飞,等.PI3K-Akt信号传导通路对糖代谢的调控作用[J].中国生物化学与分子生物学报,2010,26(10):879-885.
[21] Lai K M V,Gonzalez M,Poueymirou W T,et al.Conditional activation of Akt in adult skeletal muscle induces rapid hypertrophy[J].Molecular and Cellular Biology,2004,24(21):9295-9304.
[22] 钟宇,王亚军,李娟.猪Akt1、Akt2基因克隆与组织表达图谱分析[J].四川大学学报:自然科学版,2014,51(2):365-370.
[23] 尚胜男,李开全,柳林,等.棘皮动物结合素研究进展[J].大连海洋大学学报,2018,33(1):113-122.
[24] Lawlor M A,Alessi D R.PKB/Akt:a key mediator of cell proliferation,survival and insulin responses?[J].Journal of Cell Science,2001,114:2903-2910.
[25] 胡明英,王晓莉,毕学辉,等.RNA干扰沉默AKT基因对卵巢癌SKOV3细胞增殖与凋亡影响研究[J].中华肿瘤防治杂志,2013,20(24):1869-1873.
[26] Liang Jiyong,Slingerland J M.Multiple roles of the PI3K/PKB (Akt) pathway in cell cycle progression[J].Cell Cycle,2003,2(4):339-345.
[27] 陈一飞.中国卤虫胚胎滞育解除过程中akt/pkb基因的作用及表达模式[D].大连:辽宁师范大学,2013.
[28] 王金凤,胡鹏,牛虹博,等.低温胁迫对鱼类PI3K/AKT/GSK-3β信号通路的影响[J].生物学杂志,2016,33(6):24-28.
[29] 田志环,焦传珍,成永旭,等.中华绒螯蟹Akt基因的cDNA克隆、序列分析及表达特征[J].水产学报,2018,42(4):485-494.
[30] 于鸿燕.草鱼三个PI3K/AKT通路相关基因的克隆和表达分析[D].上海:上海海洋大学,2015.
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