摘要
[目的]鉴定河北省番茄上主要病毒病种类,明确病原和分布。[方法]采用分子生物学技术及免疫试纸条技术,对2016—2018年采自河北省8个县市18个番茄主产区的番茄样品进行病毒病种类鉴定。[结果]采集的番茄样品中共检出3种病毒,分别为番茄黄化曲叶病毒(tomato yellow leaf curl virus)、番茄褪绿病毒(tomato chlorosis virus)、番茄花叶病毒(tomato mosaic virus)。其中TYLCV是目前河北省危害最严重、分布最广泛的一种病毒,各调查地区均能检出,检出率为63.29%,其次为ToCV,检出率为17.19%,田间主要表现与番茄黄化曲叶病毒混合侵染;ToMV检出率仅为0.09%,为零星发生。[结论]河北省番茄病毒病的主要种类为粉虱传播的番茄黄化曲叶病毒和番茄褪绿病毒。
[Objective] To determine the pathogens and distribution of tomato viral diseases in Hebei Province. [Method] In 2016-2018, a total of 346 infected tomato plant samples were collected from 18 main production areas of tomato in Hebei Province. Molecular biology and immunostrip techniques were used to detect the pathogens of tomato samples. [Result] Three kinds of tomato viruses were identified in the samples, they were tomato yellow leaf curl virus(TYLCV),tomato chlorosis virus(ToCV) and tomato mosaic virus(ToMV). TYLCV could be detected at every sampling site with the highest detection rate of 63.29%. The detection rate of ToCV was 17.19%, and all the positive samples were mix-infected with TYLCV. ToMV occurred sporadically in Hebei Province with a detection rate of only 0.09%. [Conclusion] Three virus were detected, TYLCV and ToCV which transmitted by whiteflies were the main types of tomato viruses in Hebei Province.
引文
[1] 冯兰香,蔡少华,郑贵彬,等.我国番茄病毒病的主要毒原种类和番茄上烟草花叶病毒株系的鉴定[J].中国农业科学,1987,20(3):60-66.
[2] 赵世民,李秀生,谈文.番茄病毒病调查及其毒源种类的鉴定[J].河南农业大学学报,1991,25(4):407-414.
[3] 邸垫平,攻焕然,张书敏,等.河北省发生一种番茄新病害-番茄黄化曲叶病毒病[C]//成卓敏.粮食安全与植保科技创新.北京:中国农业科学技术出版社,2009:207-209.
[4] 孙国珍,高利利,陆文利,等.河北省设施番茄褪绿病毒分子检测和鉴定研究[J].北方园艺,2015(9):95-98.
[5] 刘玉乐,蔡健和,李冬玲,等.中国番茄黄化曲叶病毒——双生病毒的一个新种[J].中国科学(C辑),1998,28(2):148-153.
[6] 周莹,尚巧霞,罗晨,等.番茄黄化曲叶病毒的鉴定与群体进化分析[J].植物保护 2016,42(1):106-111.
[7] 于翠.番茄花叶病毒(ToMV)和黄瓜花叶病毒(CMV)单克隆抗体制备及ToMV在烟草上致病分子机理研究[D].杭州:浙江大学,2004.
[8] 姜玉霞.新疆加工番茄上番茄花叶病毒的分子检测及全序列测定[D].石河子:石河子大学,2008.
[9] 朱敏,王泊婷,黄莹,等.云南天竺葵上发现番茄斑萎病毒[J].南京农业大学学报,2017,40(3):450-456.
[10] 刘勇,李媛媛,吴竹妍,等.广州辣椒上番茄斑萎病毒属病毒的鉴定[J].植物保护学报,2010,37(4):383-384.
[11] 李飞,吴青君,徐宝云,等.北京地区发现番茄斑萎病毒[J].植物保护,2012,38(6):186-188.
[12] 李洁,迟胜起,杨勤民,等.山东烟台地区发生番茄斑萎病毒病危害[J].植物保护,2017,43(1):228-232.
[13] 曹金强,谢学文,柴阿丽,等.李宝聚博士诊病手记(九十三) 番茄斑萎病毒病在宁夏的发现与防治[J].中国蔬菜,2016(4):87-89.