石仙桃药材HPLC指纹图谱的建立及聚类分析
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摘要
目的:建立石仙桃药材的高效液相色谱(HPLC)指纹图谱并进行聚类分析,为石仙桃药材的质量评价提供参考。方法:根据《中药色谱指纹图谱相似度评价系统》(2012版)建立广西、广东和福建产16批石仙桃药材的HPLC指纹图谱[色谱柱为Agilent SB-aq,流动相为乙腈-0.05%磷酸水溶液(梯度洗脱),流速为1.0 mL/min,检测波长为220 nm,柱温为30℃,进样量为5μL],并采用SPSS 22.0统计学软件对其进行聚类分析。结果:根据建立的HPLC指纹图谱,共确定了11个共有峰,并指认了其中的共有色谱峰1为天麻素;16批样品的相似度均大于0.9。16批药材样品可聚为2类。结论:建立的HPLC指纹图谱和聚类分析结果可为石仙桃药材的质量控制提供一定的参考。
OBJECTIVE:To establish HPLC fingerprint of Pholidota chinensis and cluster analysis the samples,and to provide reference for quality evaluation of P. chinensis. METHODS:HPLC fingerprints of 16 batches of P. chinensis from Guangxi,Guangdong and Fujian were established according to 2012 edition of Similarity Evaluation System for Chromatographic Fingerprint of TCM [chromatographic column was Agilent SB-aq column with mobile phase consisted of acetonitrile-0.05% phosphoric acid solution(gradient elution),at the flow rate of 1.0 mL/min. The detection wavelength of 220 nm,the column temperature was30 ℃ and the sample volume was 5 μL].The cluster analysis was carried out by using SPSS 22.0 statistical software. RESULTS:Accoding to the established HPLC fingerprint,11 common peaks were indentified,and the common peak 1 was identified as gastrodin;the similarity of 16 batch of samples was greater than 0.9. 16 batches of medicinal materials could be divided into 2 groups. CONCLUSIONS:The established HPLC fingerprint and cluster analysis can provide reference for the quality control of P. chinensis.
OBJECTIVE:To establish HPLC fingerprint of Pholidota chinensis and cluster analysis the samples,and to provide reference for quality evaluation of P. chinensis. METHODS:HPLC fingerprints of 16 batches of P. chinensis from Guangxi,Guangdong and Fujian were established according to 2012 edition of Similarity Evaluation System for Chromatographic Fingerprint of TCM [chromatographic column was Agilent SB-aq column with mobile phase consisted of acetonitrile-0.05% phosphoric acid solution(gradient elution),at the flow rate of 1.0 mL/min. The detection wavelength of 220 nm,the column temperature was30 ℃ and the sample volume was 5 μL].The cluster analysis was carried out by using SPSS 22.0 statistical software. RESULTS:Accoding to the established HPLC fingerprint,11 common peaks were indentified,and the common peak 1 was identified as gastrodin;the similarity of 16 batch of samples was greater than 0.9. 16 batches of medicinal materials could be divided into 2 groups. CONCLUSIONS:The established HPLC fingerprint and cluster analysis can provide reference for the quality control of P. chinensis.
引文
[1]国家中医药管理局《中华本草》编委会.中华本草[M].上海:上海科学技术出版社.1999:748-750.
[2]李经纬,余瀛鳌,永欣,等.中医大辞典[M].北京:人民卫生出版社,1995:439.
[3]王晓燕,黎理,朱华.石仙桃研究进展[J].亚太传统医药,2016,12(1):42-44.
[4]刘建新,周青,连其深.石仙桃对中枢神经系统抑制作用[J].赣南医学院学报,2004,24(2):119-121.
[5]杨素雄,姜家康,迟文成,等.石仙桃的药理进展[J].世界最新医学信息文摘,2016,16(69):67-68,70.
[6]林礒石,严德志.头痛定糖浆对神经机能性头痛的疗效观察[J].福建医药杂志,1985,7(6):28.
[7]朱华.中国壮药志:第1卷[M].南宁:广西民族出版社,2003:171-174.
[8]张淼,朱华,陈龙,等.石仙桃生药学鉴定[J].时珍国医国药,2018,29(9):2177-2179.
[9]黎理,曾祥燕,谢凤凤,等.竹节蓼药材的HPLC指纹图谱建立及聚类分析[J].中国药房,2018,29(12):1640-1643.
[10]孙绪,姚成芬,付思红,等.苗药朱砂根的HPLC指纹图谱研究[J].中国药房,2017,28(30):4285-4288.
[11]张洪峰,张学强,于丽霞,等.基于HPLC指纹图谱与聚类分析对不同产地连翘质量评价[J].江苏中医药,2018,50(4):78-80.
[12]李坤,李明花,秦文杰,等.基于高效液相指纹图谱及聚类分析对不同产地半夏质量评价研究[J].中南药学,2018,16(12):1750-1754.
[2]李经纬,余瀛鳌,永欣,等.中医大辞典[M].北京:人民卫生出版社,1995:439.
[3]王晓燕,黎理,朱华.石仙桃研究进展[J].亚太传统医药,2016,12(1):42-44.
[4]刘建新,周青,连其深.石仙桃对中枢神经系统抑制作用[J].赣南医学院学报,2004,24(2):119-121.
[5]杨素雄,姜家康,迟文成,等.石仙桃的药理进展[J].世界最新医学信息文摘,2016,16(69):67-68,70.
[6]林礒石,严德志.头痛定糖浆对神经机能性头痛的疗效观察[J].福建医药杂志,1985,7(6):28.
[7]朱华.中国壮药志:第1卷[M].南宁:广西民族出版社,2003:171-174.
[8]张淼,朱华,陈龙,等.石仙桃生药学鉴定[J].时珍国医国药,2018,29(9):2177-2179.
[9]黎理,曾祥燕,谢凤凤,等.竹节蓼药材的HPLC指纹图谱建立及聚类分析[J].中国药房,2018,29(12):1640-1643.
[10]孙绪,姚成芬,付思红,等.苗药朱砂根的HPLC指纹图谱研究[J].中国药房,2017,28(30):4285-4288.
[11]张洪峰,张学强,于丽霞,等.基于HPLC指纹图谱与聚类分析对不同产地连翘质量评价[J].江苏中医药,2018,50(4):78-80.
[12]李坤,李明花,秦文杰,等.基于高效液相指纹图谱及聚类分析对不同产地半夏质量评价研究[J].中南药学,2018,16(12):1750-1754.
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