婴儿血管瘤组织中微小RNA-130a、B淋巴细胞瘤-2的表达观察

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英文篇名：Expression of miR-130a and Bcl-2 in infantile hemangiomas 作者：高培培 ; 张兰成 ; 朱光辉 ; 卢彬 ; 张数青 英文作者：GAO Peipei;ZHANG Lancheng;ZHU Guanghui;LU Bin;ZHANG Shuqing;Tianjin Stomatological Hospital; 关键词：微小RNA ; 微小RNA-130a ; B淋巴细胞瘤-2 ; 血管瘤 ; 婴儿血管瘤 英文关键词：microRNA;;microRNA-130a;;B lymphocytoma-2;;hemangioma;;infant hemangioma 中文刊名：SDYY 英文刊名：Shandong Medical Journal 机构：天津市口腔医院; 出版日期：2019-06-25 出版单位：山东医药 年：2019 期：v.59;No.1140 基金：天津市自然科学基金资助项目(15JCYBJC29600) 语种：中文; 页：SDYY201918005 页数：4 CN：18 ISSN：37-1156/R 分类号：24-27

摘要

目的观察婴儿血管瘤(Infantile hemangioma,IH)组织中微小RNA(micro RNA,miR)-130a、B淋巴细胞瘤-2(Bcl-2)的表达变化。方法 33例IH患者,其中增殖期17例、退化期16例,留取IH组织、瘤旁正常皮肤组织。采用荧光实时定量PCR法检测IH组织、瘤旁正常皮肤组织中miR-130a和Bcl-2 mRNA,采用SP免疫组织化学法检测IH组织、瘤旁正常皮肤组织BCl-2蛋白,分析IH组织miRNA-130a、Bcl-2 mRNA表达的相关性。结果 IH组织、瘤旁正常皮肤组织中miR-130a相对表达量分别为3. 71±1. 23、0. 87±0. 21,退化期、增殖期IH组织miRNA-130a相对表达量分别为3. 68±1. 40、3. 72±1. 63; IH组织及瘤旁正常皮肤组织Bcl-2 mRNA相对表达量分别为1. 213±0. 272、0. 614±0. 22,退化期、增殖期IH组织Bcl-2 mRNA相对表达量比较分别为3. 68±1. 40、3. 72±1. 63,与瘤旁正常皮肤组织比较,退化期、增殖期IH组织miRNA-130a及Bcl-2 mRNA相对表达量均升高(P均<0. 05)。与瘤旁正常皮肤组织比较,IH组织Bcl-2蛋白光密度值和阳性面积率均升高(P均<0. 05);与退化期IH组织、瘤旁正常皮肤组织比较,增殖期IH组织Bcl-2蛋白光密度值和阳性面积率均升高(P均<0. 05)。IH组织中miR-130a与Bcl-2 mRNA的表达呈正相关(r=0. 451,P=0. 008)。结论 IH组织miR-130a、Bcl-2 mRNA及蛋白表达均升高,增殖期IH组织Bcl-2蛋白表达高于退化期。miR-130a、Bcl-2可能协同促进IH的发生、发展。
        Objective To observe the expression of microRNA-130 a( miR-130 a) and B lymphocytoma-2( Bcl-2) in infantile hemangioma( IH). Methods Thirty-one cases of patients with IH were enrolled,including 17 cases in proliferative stage and 16 cases in degenerative stage. The expression of miR-130 a and Bcl-2 in IH tissues and adjacent normal skin tissues were detected by real-time quantitative fluorescence PCR( qRT-PCR). Bcl-2 protein in IH tissues and adjacent normal skin tissues was detected by SP immunohistochemistry. The correlation between the expression of miR-130 a and Bcl-2 in IH tissues were analyzed. Results The relative expression levels of miR-130 a in IH tissues and adjacent normal skin tissues were 3. 71 ± 1. 23 and 0. 87 ± 0. 21,respectively. The relative expression levels of miRNA-130 a in the degenerative and proliferative IH tissues were 3. 68 ± 1. 40 and 3. 72 ± 1. 63,respectively. The relative expression levels of Bcl-2 mRNA in the IH tissues and adjacent normal skin tissues were 1. 213 ± 0. 272 and 0. 614 ± 0. 22,respectively. The relative expression levels of Bcl-2 mRNA in the degenerative and proliferative IH tissues were 3. 68 ± 1. 40 and 3. 72 ± 1. 63,respectively. The relative expression levels of miR-130 a and Bcl-2 mRNA in the IH tissues were significantly higher than those in adjacent normal skin tissues( all P < 0. 05). Bcl-2 protein optical density and positive area in the IH tissues were higher than those in the adjacent normal skin tissues( all P < 0. 05); Bcl-2 protein optical density and positive area in IH tissues during proliferation were higher than those in degenerative IH tissues and adjacent normal skin tissues( all P < 0. 05).There was a positive correlation between the expression of miR-130 a and Bcl-2 mRNA in IH tissues( r = 0. 451,P =0. 008). Conclusions The expression levels of and Bcl-2 in IH tissues increase,and the expression of Bcl-2 in IH tissues during proliferation was higher than that in degeneration. miR-130 a and Bcl-2 may synergistically promote the occurrence and development of IH.

引文

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