基于药效实验和层次分析法的参芪强心片提取工艺优选

详细信息    查看全文 | 推荐本文 |

英文篇名：Optimization of extraction process of Shenqi Qiangxin Tablets based on pharmacodynamic test and analytic hierarchy process 作者：莫平莉 ; 王洛临 ; 卢泳 ; 李洁环 ; 冯健英 ; 陈雪婷 ; 徐文杰 ; 李智勇 英文作者：MO Ping-li;WANG Luo-lin;LU Yong;LI Jie-huan;FENG Jian-ying;CHEN Xue-ting;XU Wen-Jie;LI Zhi-yong;The Fifth Clinical Medicine School Affiliated to Guangzhou University of Chinese Medicine;Guangdong Provincial Engineering Technology Research Institute of Traditional Chinese Medicine/Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine; 关键词：参芪强心片 ; 心力衰竭 ; 提取工艺 ; 层次分析 ; 单因素实验 ; 正交试验 ; 人参皂苷Rg1 ; 人参皂苷Re ; 淫羊藿苷 ; 红参 ; 黄芪 ; 淫羊藿 英文关键词：Shenqi Qiangxin Tablets;;heart failure;;extraction technology;;analytic hierarchy process;;single factor test;;orthogonal test;;ginsenoside Rg1;;ginsenoside Re;;icariin;;red ginseng;;Astragalus membranaceus(Fisch.) Bunge;;Epimedium brevicornu Maxim 中文刊名：ZCYO 英文刊名：Chinese Traditional and Herbal Drugs 机构：广州中医药大学第五临床医学院;广东省中医药工程技术研究院/广东省中医药研究开发重点实验室; 出版日期：2019-06-12 出版单位：中草药 年：2019 期：v.50;No.646 基金：广东省科技计划项目(2017A070701017);广东省科技计划项目(2013B040200040) 语种：中文; 页：ZCYO201911013 页数：9 CN：11 ISSN：12-1108/R 分类号：82-90

摘要

目的优化参芪强心片的提取工艺。方法采用药效实验,以对异丙肾上腺素诱发的大鼠心力衰竭模型心脏病变改善程度为指标,初步筛选制备工艺;采用层次分析法结合单因素实验、正交试验,以固形物质量、人参皂苷Rg1和人参皂苷Re含量,优化乙醇回流提取工艺;以固形物质量和淫羊藿苷含量为指标,优化煎煮提取工艺,并对提取工艺进行验证试验。结果药效实验表明工艺4更优;该工艺的最佳提取条件为红参、黄芪等5味饮片加50%乙醇回流提取3次,第1次加11倍量,第2、3次加10倍量,每次2.5 h;淫羊藿等其余2味饮片煎煮提取3次,第1次加19倍量,第2、3次加16倍量,每次1.5 h。验证试验表明乙醇提取固形物得率平均值为19.78%、人参皂苷Rg1和人参皂苷Re提取率平均值为77.52%;水提取固形物得率平均值为16.58%、淫羊藿苷提取率平均值为90.98%(RSD<2.0%,n=3)。结论该提取工艺稳定可行。
        Objective To optimize the extraction technology of Shenqi Qiangxin Tablets(SQT). Methods With the improvement of heart lesion of pharmacological model of isoproterenol induced heart failure in rats as the index, pharmacological efficacy test was used to screen extracting conditions of the technology. The extraction technology was optimized by analytic hierarchy process combined with principal component analysis, single factor and orthogonal tests using each content of solid matter, ginsenosides Rg1, Re as indexes. And the verification test was carried out by using solid mass and icariin content as indexes. Results Pharmacological efficacy test showed that technology 4 was superior. The optimal extraction condition of technology 4 was as follow: five medicinal materials including red ginseng and astragalus were reflux extracted three times with 50% ethanol, 11 fold for the first time, 10 fold for the second and three times, 2.5 h for each extraction; Epimedium and the other two medicinal materials were decocted three times with water, 19 fold for the first time, 16 fold for the second and third times, 1.5 h for each decction. The verification test showed that the average yield of ethanol extracted solids was 19.78%, and the average extraction rate of ginsenoside Rg1 and Re was 77.52%; The average value of water extracted solids was 16.58%, and the average extraction rate of epimedium was 90.98%(RSD < 2.0%, n = 3).Conclusion The optimized extraction technology was stable and feasible.

引文

[1]顾东风,黄广勇,何江,等.中国心力衰竭流行病学调查及其患病率[J].中华心血管病杂志,2003,31(1):3-6.
    [2]张奕奕,薛一涛.中医药治疗心力衰竭研究进展[J].黑龙江中医药,2014,43(4):62-64.
    [3]邓青秀,彭成.中药治疗心力衰竭的研究现状[J].中药与临床,2011,2(1):57-60.
    [4]王晓燕,李七一,严士海.异丙肾上腺素诱导慢性心力衰竭大鼠模型实验研究[J].辽宁中医药大学学报,2017,19(8):31-34.
    [5]陈向云,李尧锋,刘杨,等.苗药理气活血滴丸对慢性心力衰竭模型大鼠的保护作用研究[J].中药与民族药,2018,29(16):2233-2237.
    [6]任爱农,卢爱玲,田耀洲,等.层次分析法用于中药复方提取工艺的多指标权重研究[J].中国中药杂志,2008,33(4):372-374.
    [7]贾成友,李微,张传辉,等.基于多指标权重分析和正交设计法优选白黄泄热止痢片复方提取工艺[J].中草药,2016,47(6):917-922.
    [8]王政,魏莉.利用SPSS软件实现药学实验中正交设计的方差分析[J].数理医药杂志,2014,27(1):99-102.
    [9]中国药典[S].一部.2015.
    [10]Lim K H,Lim D J,Kim J H.Ginsenoside-Re ameliorates.Ischemia and reperfusion injury in the heart:Ahemodynamics approach[J].J Ginseng Res,2013,37(3):283-292.
    [11]孙宪昌,郭俊,姜明春,等.人参皂苷Rg1通过Akt-Nrf2信号通路拮抗顺铂诱导的豚鼠耳毒性作用研究[J].中草药,2018,49(14):3309-3317.
    [12]李渊,周玥,王亚平,等.人参皂苷Rg1基于SIRT6/NF-κB信号通路对辐射致造血干/祖细胞衰老的保护作用[J].中草药,2017,48(21):4497-4501.
    [13]向玥,陈粼波,姚辉,等.人参皂苷Rg1对D-半乳糖所致衰老小鼠海马的保护机制[J].中草药,2017,48(18):3789-3795.
    [14]孙莹莹,刘玥,陈可冀.人参皂苷的心血管药理效应:进展与思考[J].中国科学:生命科学,2016,46(6):771-778.
    [15]安明,赵国君,韦新成.人参皂苷Rg1保护心血管和中枢神经系统的药理活性研究进展[J].中国临床药理学杂志,2012,28(1):75-77.
    [16]黎阳,张铁军,刘素香,等.人参化学成分和药理研究进展[J].中草药,2009,40(1):164-附2.
    [17]高晨盈,王俊逸,罗云梅,等.人参皂苷Re对球囊损伤大鼠血管内膜增殖及TGF-β1/Smads信号通路的影响[J].中草药,2017,48(1):143-148.
    [18]李娌,王学美.淫羊藿苷药理作用研究进展[J].中国中药杂志,2008,33(23):2727-2731.
    [19]夏国莲,黄兆铨.淫羊藿对心血管系统药理作用的研究新进展[J].中华中医药学刊,2010,28(8):1676-1679.
    [20]胡长明,黄露,许明旺,等.基于层次分析权数法的参葛降脂颗粒提取工艺优选[J].中国医院药学杂志,2015,35(24):2204-2208.