摘要
目的优选养阴清肺汤加味提取工艺。方法采用HPLC法同时测定毛蕊花糖苷、丹皮酚、绿原酸、甘草酸,以层次分析法(AHP)计算权重系数,多指标综合评分结合Box-Behnken设计-响应面法考察乙醇体积分数、提取时间、提取次数对养阴清肺汤加味提取工艺的影响,优选醇提工艺。结果最佳提取工艺为采用69%乙醇,提取68min,提取1次。结论优选的提取工艺简单可行,成分提取效率高,为养阴清肺汤加味提取工艺提供参考。
Objective To optimize the extraction process of Modified Yangyin Qingfei Decoction(MYQD). Methods The effects of ethanol concentration, extraction time and extraction times on the optimization of extraction process of MYQD were investigated by multiple indicators comprehensive scoring and Box-Behnken response surface methodology. The content of verbascoside, chlorogenic acid, paeonol, and glycyrrhizic acid was simultaneously determined by HPLC, and the method of analytic hierarchy process was used to calculate the weight coefficient. Results The optimum extraction process was as follow: using 69% ethanol for once extraction for 68 min. Conclusion The optimum extraction process is simple and feasible, and the extraction efficiency of components is high,which can provide reference for the extraction process of MYQD.
引文
[1]徐玲霞,刘水婷,刘骏,等.Box-Behnken效应面法优化吴茱萸次碱脂质液晶纳米粒的处方研究[J].中草药,2018,49(21):5076-5081.
[2]黄潇,刘婧,彭水梅,等.响应面法优化栀子中总多酚、总黄酮的提取工艺[J].中国药房,2017,28(28):3964-3968.
[3]王冲,赵明芬,陈良,等.Box-Behnken响应面法优化排闼宗阳颗粒提取工艺[J].中国药师,2019,22(1):151-155.
[4]宋燕,冉姗,孙方方,等.Box-Behnken设计-响应面法优化木鳖子霜炮制工艺[J].中草药,2019,50(2):382-387.
[5]万丹,张水寒,肖娟,等.Box-Behnken设计-效应面法优选酒黄连炮制工艺[J].药物评价研究,2014,37(4):341-345.
[6]刘小妹,程中琴,施崇精,等.基于AHP-CRITIC法的正交设计优选参膝口服液提取工艺[J].中草药,2018,49(11):2577-2583.
[7]石振武,赵敏.运用层次分析法确定指标的权值[J].科技和产业,2008,8(2):23-25.
[8]李炜.养阴清肺汤联合孟鲁司特治疗小儿支原体肺炎致慢性咳嗽临床研究[J].新中医,2019,51(1):70-73.
[9]付慧玲.中西医结合治疗儿童肺炎支原体肺炎疗效观察[J].中国民间疗法,2016,24(10):76-77.
[10]刘宪宾.养阴清肺汤加减联合金嗓散结丸对慢性咽炎患者症状及免疫功能的影响[J].河南医学研究,2018,27(18):3362-3363.
[11]Pastore S,Lulli D,Fidanza P,et al.Plant polyphenols regulate chemokine expression and tissue repair in human keratinocytes through interaction with cytoplasmic and nuclear components of epidermal growth factor receptor system[J].Antioxid Redox Sign,2012,16(4):314-328.
[12]蒋丽丽,张彦龙,王春杰,等.牡丹皮中有效成分丹皮酚的药理活性研究进展[J].现代诊断与治疗,2016,27(22):4223-4224.
[13]崔虹,朱佳茜,冯秋芳,等.中药白芍化学成分及生物活性研究进展[J].海峡药学,2017,29(9):1-5.
[14]魏明,杨晓梅,刘佳红,等.绿原酸的药理作用研究进展[J].陕西中医,2016,37(4):511-512.
[15]严永旺,肖兰,周旭,等.绿原酸的药理作用及药用研发对策[J].中国药房,2017,28(19):2729-2732.
[16]张明发,沈雅琴.甘草及其活性成分抗炎与抗炎机制的研究进展[J].现代药物与临床,2011,26(4):261-268.
[17]张利.甘草的药理作用及现代研究进展[J].中医临床研究,2014,6(10):147-148.
[18]蔡志明,刘颜,王光明,等.医院绩效评估指标体系权重研究[J].中国卫生经济,2004,23(8):34-35.
[19]陈勇,陈明,王钧,等.基于灰色关联分析法辨识中药生产过程关键工艺参数[J].中草药,2019,50(3):582-587.
[20]时维静,王海侠,卜先峰,等.综合评分法优化白头翁汤提取工艺[J].中国中医药科技,2009,16(1):44-45.