疏肝调神针灸法对创伤后应激障碍致睡眠障碍大鼠海马区异常神经信息编码时空模式及受损神经元超微结构影响
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摘要
目的:分析疏肝调神针灸法对创伤后应激障碍(PTSD)致睡眠障碍大鼠海马区异常神经信息编码时空模式及受损神经元超微结构影响。方法:选取SPF级Wistar雄性大鼠45只,随机数字表法将大鼠分成三组,观察组、模型组与正常组,每组各15只,观察组与模型组大鼠制备PTSD模型,钻颅法埋置电极并记录大鼠非快速动眼睡眠期(NREMS)及快速动眼睡眠期(REMS)时间,检测大鼠海马CA3与CA1区域内神经元的放电波幅数值及超微结构状况。结果:模型组大鼠NERMS及REMS潜伏期比正常组上升,观察组比模型组下降,差异均有统计学意义(P<0.05);模型组大鼠海马CA1、CA3波幅数值比正常组下降,观察组比模型组上升,差异有统计学意义(P<0.05)。结论:疏肝调神针灸法可显著改善PTSD睡眠障碍大鼠的睡眠质量,逆转其海马区神经元的放电活动并恢复神经元细胞结构。
Objective:To analyze the effect of acupuncture and moxibustion on the spatial and temporal patterns of abnormal neuronal information in the hippocampus of rats with sleep disorder induced by post-traumatic stress disorder(PTSD) and the ultrastructure of damaged neurons.Methods:A total of 45 male Wistar male rats were randomly divided into three groups.The observation group, the model group and the normal group, 15 rats in each group.The PTSD model was prepared by the observation group and the model group.The cranial method was used to embed the electrodes and the non-rapid eye movement sleep period(NREMS) and rapid eye movement sleep(REMS) time were recorded.The amplitude and ultrastructure of the neurons in the hippocampal CA3 and CA1 regions were measured.Results:The latency of NERMS and REMS in the model group was higher than that in the normal group.The difference between the observation group and the model group was statistically significant(P<0.05).The amplitude of CA1 and CA3 in the hippocampus of the model group was lower than that of the normal group.The group was higher than the model group, and the difference was statistically significant(P<0.05).Conclusion:Shugan Tiaoshen acupuncture can significantly improve the sleep quality of rats with PTSD sleep disorder, reverse the discharge activity of neurons in hippocampus and restore the structure of neuron cells.
Objective:To analyze the effect of acupuncture and moxibustion on the spatial and temporal patterns of abnormal neuronal information in the hippocampus of rats with sleep disorder induced by post-traumatic stress disorder(PTSD) and the ultrastructure of damaged neurons.Methods:A total of 45 male Wistar male rats were randomly divided into three groups.The observation group, the model group and the normal group, 15 rats in each group.The PTSD model was prepared by the observation group and the model group.The cranial method was used to embed the electrodes and the non-rapid eye movement sleep period(NREMS) and rapid eye movement sleep(REMS) time were recorded.The amplitude and ultrastructure of the neurons in the hippocampal CA3 and CA1 regions were measured.Results:The latency of NERMS and REMS in the model group was higher than that in the normal group.The difference between the observation group and the model group was statistically significant(P<0.05).The amplitude of CA1 and CA3 in the hippocampus of the model group was lower than that of the normal group.The group was higher than the model group, and the difference was statistically significant(P<0.05).Conclusion:Shugan Tiaoshen acupuncture can significantly improve the sleep quality of rats with PTSD sleep disorder, reverse the discharge activity of neurons in hippocampus and restore the structure of neuron cells.
引文
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[2] Giannoni-Pastor A,Eiroa-Orosa F J,Fidel Kinori S G,et al.Prevalence and Predictors of Posttraumatic Stress Symptomatology Among Burn Survivors:A Systematic Review and Meta-Analysis[J].Journal of Burn Care Research,2016,37(1):79～83
[3] 李越峰,张泽国,徐富菊,等.从肝论治创伤后应激障碍及睡眠障碍的研究进展[J].中国中医药信息杂志,2016,23(7):128～131
[4] 王承展,黄庚娣,方青,等.创伤性应激障碍样大鼠海马GABA受体表达的变化[J].中国心理卫生杂志,2016,30(12):952～956
[5] 王禾,廖维宏,吴宝明,等.创伤后应激障碍大鼠动物模型行为学表现及学习记忆功能的变化[J].中国组织工程研究,2004,8(10):1848～1850
[6] 郭义,方剑乔.实验针灸学[M].北京:中国中医药出版社,2012:124～128
[7] 帕克森诺斯.大鼠脑立体定位图谱[M].北京:人民卫生出版社,2005:64～68
[8] Divita C,George S,Barr C J.Traumatic brain injury and post-traumatic amnesia:a retrospective review of discharge outcomes[J].Brain Injury,2017,9(7):1～4
[9] 吴仲敏,程正文,倪桂莲,等.人参皂苷 Rg1对创伤后应激障碍大鼠行为学变化和海马神经元自噬的影响[J].中国病理生理杂志,2017,33(5):896～901
[10] 李国正,钟文波,张志斌,等.驻疆官兵创伤后应激障碍及睡眠障碍的调查[J].神经损伤与功能重建,2018,5(3):204～208
[11] 曹瑞,牛江涛,李越峰.四逆散对创伤后应激障碍大鼠海马组织分子表达的作用[J].中国临床药理学杂志,2017,33(10):921～924
[12] 李凤蕾,杜菲,吴迪,等.乌灵菌粉早期应用对创伤后应激障碍模型大鼠行为及海马组织中IL-1β、IL-6水平的影响[J].中国药房,2016,27(25):3478～3480
[13] Katona L,Micklem B,Borhegyi Z,et al.Behavior-dependent activity patterns of GABAergic long-range projecting neurons in the rat hippocampus[J].Hippocampus,2017,27(4):359～364
[14] 邢文龙,王梅子,时若欢,等.催产素对创伤后应激障碍样大鼠行为学及海马MKP-1表达的影响[J].中华行为医学与脑科学杂志,2018,7(3):208～215
[15] Terada S,Sakurai Y,Nakahara H,et al.Temporal and Rate Coding for Discrete Event Sequences in the Hippocampus[J].Neuron,2017,94(6):1248～1262
[16] 闫海江.创伤后应激障碍与慢性痛共病的机制:海马小胶质细胞的激活[J].中国疼痛医学杂志,2018,9(6):61～64
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