玉米高油型诱导系CHOI1和CHOI3的单倍体鉴定效率评价
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摘要
为系统评价新型高油型诱导系油份鉴定单倍体的效率,以籽粒油分含量分别为8.71%和8.72%的2个高油型诱导系CHOI1和CHOI3为父本,分别在海南省和北京市对10个普通玉米杂交种进行诱导,利用油分和R1-nj遗传标记对诱导产生的籽粒分别进行单倍体的鉴定,并对2种鉴定方法进行评价。结果表明:与遗传标记法相比,油分标记法的单倍体鉴定准确率更高,且不易受环境影响。CHOI1和CHOI3诱导杂交籽粒与其母本单倍体籽粒间的平均油分均值差异分别为2.21%和2.02%,变异范围分别为1.86%～2.63%,1.54%～2.46%;各组合诱导率平均值分别为8.30%和8.59%,变异范围分别为6.25%～10.40%,5.81%～11.19%。诱导系CHOI1作父本时,以油份均值(μ)+1.645×标准差(σ)作为区分单倍体的阈值,所测组合的单倍体鉴定准确率均高于90%,漏选率均低于10%。以4.0%的籽粒油分含量为通用阈值,所测组合均可达到90%以上的鉴定准确率。
The objective of this study was to systematically evaluate the efficiency of haploid identification by oil content(OC) with new High-Oil Inducers.Ten hybrids were induced by two High-Oil Inducers CHOI1 and CHOI3 with OC 8.71% and 8.72% respectively in Beijing and Hainan.The haploid identification by OC and R1-nj markers were evaluated and compared.The results showed that the accuracy rate(AR) of OC system,which was not influenced by environments,was higher than that of R1-nj system.When OC system was used to identify haploids induced by CHOI1 and CHOI3,the difference in the mean OC of haploids and crossing seeds were respectively 2.21% and 2.02% and the ranges of variation were 1.86%-2.63% and 1.54%-2.46%,respectively.The haploid induction rates were 8.30% and 8.59%,with ranges of 6.25%-10.40% and 5.81%-11.19%,respectively.When indexes mean(μ) +1.645×standard deviation(σ) was used as the oil content threshold value to classify the haploids induced by CHOI1,the ARs were all beyond 90%.In addition,the missing rates were all below 10%.The ARs of all the crosses were more than 90% by using 4% seed oil as the general threshold.
The objective of this study was to systematically evaluate the efficiency of haploid identification by oil content(OC) with new High-Oil Inducers.Ten hybrids were induced by two High-Oil Inducers CHOI1 and CHOI3 with OC 8.71% and 8.72% respectively in Beijing and Hainan.The haploid identification by OC and R1-nj markers were evaluated and compared.The results showed that the accuracy rate(AR) of OC system,which was not influenced by environments,was higher than that of R1-nj system.When OC system was used to identify haploids induced by CHOI1 and CHOI3,the difference in the mean OC of haploids and crossing seeds were respectively 2.21% and 2.02% and the ranges of variation were 1.86%-2.63% and 1.54%-2.46%,respectively.The haploid induction rates were 8.30% and 8.59%,with ranges of 6.25%-10.40% and 5.81%-11.19%,respectively.When indexes mean(μ) +1.645×standard deviation(σ) was used as the oil content threshold value to classify the haploids induced by CHOI1,the ARs were all beyond 90%.In addition,the missing rates were all below 10%.The ARs of all the crosses were more than 90% by using 4% seed oil as the general threshold.
引文
[1] 陈绍江,黎亮,李浩川.玉米单倍体育种技术[M].北京:中国农业大学出版社,2009Chen S J,Li L,Li H C.Maize Doubled-Haploid Technology[M].Beijing:China Agricultural University Press,2009 (in Chinese)
[2] 王元东,张华生,段民孝,张春原,张雪原,刘新香,陈传永,赵久然.玉米新品种NK971的选育及栽培制种技术要点[J].中国种业,2016(2):54-55Wang Y D,Zhang H S,Duan M X,Zhang C Y,Zhang X Y,Liu X X,Chen C Y,Zhao J R.Breeding and cultivation techniques of new maize variety NK971[J].China Seed Industry,2016(2):54-55 (in Chinese)
[3] 张亚勤.高产优质大穗型玉米新品种渝单50通过重庆市审定[J].植物医生,2016,31(7)Zhang Y Q.A new maize variety Yu Dan 50 with high yield,good quality and large ears was approved by Chongqing[J].Plant Doctor,2016,31(7) (in Chinese)
[4] Nanda D K,Chase S S.An embryo marker for detecting monoploids of maize (Zea mays L)[J].Crop Science,1966,6(2):213-215
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[6] R?ber F K,Gordillo G A,Geiger H H.In vivo haploid induction in maize-performance of new inducers and significance of doubled haploid lines in hybrid breeding[J].Maydica,2005,50(3):275-283
[7] 陈绍江,宋同明.利用高油分的花粉直感效应鉴别玉米单倍体[J].作物学报,2003,29(4):587-590Chen S J,Song T M.Identification haploid with high oil xenia effect in maize[J].Acta Agronomica Sinica,2003,29(4):587-590 (in Chinese)
[8] Melchinger A E,Schipprack W,Würschum T,Chen S,Technow F.Rapid and accurate identification of in vivo-induced haploid seeds based on oil content in maize[J].Scientific Reports,2013,3(3):2129
[9] Liu J,Guo T T,Li H C,Jia S Q,Yan Y L,An D,Zhang Y,Chen S J.Discrimination of maize haploid seeds from hybrid seeds using vis spectroscopy and support vector machine method[J].Spectroscopy & Spectral Analysis,2015,35(11):3268-3274
[10] Dong X,Xu X,Li L,Liu C X,Tian X L,Li W,Chen S J.Marker assisted selection and evaluation of high oil in vivo haploid inducers in maize[J].Molecular Breeding,2014,34(3):1147-1158
[11] Antoine-Michard S,Beckert M.Spontaneous versus colchicine-induced chromosome doubling in maize anther culture[J].Plant Cell,Tissue and Organ Culture,1997,48(3):203-207
[12] Barret P,Brinkmann M,Beckert M.A major locus expressed in the male gametophyte with incomplete penetrance is responsible for in situ gynogenesis in maize[J].Theoretical and Applied Genetics,2008,117(4):581-594
[13] Li L,Xu X,Jin W,Chen S J.Morphological and molecular evidences for DNA introgression in haploid induction via a high oil inducer CAUHOI in maize[J].Planta,2009,230(2):367-376
[14] Zhao X,Xu X,Xie H,Chen S,Jin W.Fertilization and uniparental chromosome elimination during crosses with maize haploid inducers[J].Plant Physiology,2013,163(2):721-731
[15] Belicuas P R,Guimar?es C T,Paiva L V,Duarte J M,Maluf W R,Paiva E.Androgenetic haploids and SSR markers as tools for the development of tropical maize hybrids[J].Euphytica,2007,156(1-2):95-102
[2] 王元东,张华生,段民孝,张春原,张雪原,刘新香,陈传永,赵久然.玉米新品种NK971的选育及栽培制种技术要点[J].中国种业,2016(2):54-55Wang Y D,Zhang H S,Duan M X,Zhang C Y,Zhang X Y,Liu X X,Chen C Y,Zhao J R.Breeding and cultivation techniques of new maize variety NK971[J].China Seed Industry,2016(2):54-55 (in Chinese)
[3] 张亚勤.高产优质大穗型玉米新品种渝单50通过重庆市审定[J].植物医生,2016,31(7)Zhang Y Q.A new maize variety Yu Dan 50 with high yield,good quality and large ears was approved by Chongqing[J].Plant Doctor,2016,31(7) (in Chinese)
[4] Nanda D K,Chase S S.An embryo marker for detecting monoploids of maize (Zea mays L)[J].Crop Science,1966,6(2):213-215
[5] Prasanna B M,Chaikam V,Mahuku G.Doubled Haploid Technology in Maize Breeding:Theory and Practice[M].Mexico,D.F:Centro Internacional de Mejoramientode Maizy Trigo Press,2012
[6] R?ber F K,Gordillo G A,Geiger H H.In vivo haploid induction in maize-performance of new inducers and significance of doubled haploid lines in hybrid breeding[J].Maydica,2005,50(3):275-283
[7] 陈绍江,宋同明.利用高油分的花粉直感效应鉴别玉米单倍体[J].作物学报,2003,29(4):587-590Chen S J,Song T M.Identification haploid with high oil xenia effect in maize[J].Acta Agronomica Sinica,2003,29(4):587-590 (in Chinese)
[8] Melchinger A E,Schipprack W,Würschum T,Chen S,Technow F.Rapid and accurate identification of in vivo-induced haploid seeds based on oil content in maize[J].Scientific Reports,2013,3(3):2129
[9] Liu J,Guo T T,Li H C,Jia S Q,Yan Y L,An D,Zhang Y,Chen S J.Discrimination of maize haploid seeds from hybrid seeds using vis spectroscopy and support vector machine method[J].Spectroscopy & Spectral Analysis,2015,35(11):3268-3274
[10] Dong X,Xu X,Li L,Liu C X,Tian X L,Li W,Chen S J.Marker assisted selection and evaluation of high oil in vivo haploid inducers in maize[J].Molecular Breeding,2014,34(3):1147-1158
[11] Antoine-Michard S,Beckert M.Spontaneous versus colchicine-induced chromosome doubling in maize anther culture[J].Plant Cell,Tissue and Organ Culture,1997,48(3):203-207
[12] Barret P,Brinkmann M,Beckert M.A major locus expressed in the male gametophyte with incomplete penetrance is responsible for in situ gynogenesis in maize[J].Theoretical and Applied Genetics,2008,117(4):581-594
[13] Li L,Xu X,Jin W,Chen S J.Morphological and molecular evidences for DNA introgression in haploid induction via a high oil inducer CAUHOI in maize[J].Planta,2009,230(2):367-376
[14] Zhao X,Xu X,Xie H,Chen S,Jin W.Fertilization and uniparental chromosome elimination during crosses with maize haploid inducers[J].Plant Physiology,2013,163(2):721-731
[15] Belicuas P R,Guimar?es C T,Paiva L V,Duarte J M,Maluf W R,Paiva E.Androgenetic haploids and SSR markers as tools for the development of tropical maize hybrids[J].Euphytica,2007,156(1-2):95-102
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