干旱胁迫下野生大豆ABC转运蛋白转录组测序分析
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摘要
为获取干旱胁迫状态下ABC转运蛋白家族相关基因,以抗旱性强的野生大豆为试验材料,浇灌20%PEG 6000模拟干旱胁迫,分别在干旱处理0、6、12、24和48 h后取叶片提取RNA进行转录组测序;以GO、KEGG和NR三大数据库注释的结果为基础,再以ABC转运蛋白为关键词进行筛选。结果表明,共获得337条ABC转运蛋白相关序列,包括8大亚家族(ABCA、ABCB、ABCC、ABCD、ABCE、ABCF、ABCG和ABCI)。差异表达分析结果表明,共有182条表现差异表达。GO注释到的差异表达基因多数为嘌呤核苷酸分解过程,KEGG注释到的差异表达基因分布在5个亚家族(ABCA、ABCB、ABCC、ABCD和ABCG)。对NR数据库有注释的168条差异表达基因进行进化分析,结果表明ABCE、ABCF、ABCG家族与ABCI11为一类,ABCB、ABCC家族与ABCI10为一类,ABCA、ABCD家族与ABCI19为一类。本研究为进一步研究野生大豆干旱胁迫下ABC转运蛋白抗旱基因提供了一定的理论依据。
In order to obtain the genes related to ABC transporter protein family under the conditions of drought stress,the drought-resistant wild soybean accession was used as experimental material which was treated with 20% PEG6000 solution to simulate drought stress. RNA was extracted from leaves of drought treated 0,6,12,24 and 48 h respectively,and transcriptome sequencing was carried out by Illumina Hi Seq 2000 platform. Based on the results of GO,KEGG and NR 3 major database notes,and then using ABC transporter as the key words to screen,a total of 337 ABC transporter related sequences were obtained,including 8 subfamilies( ABCA、ABCB、ABCC、ABCD、ABCE、ABCF、ABCG 和 ABCI). Differential expression analysis showed that there were 182 differentially expressed genes. Most of the differentially expressed genes annotated by GO database were purine ribonucleotide catabolic processes,while the Unigenes annotated by KEGG database were distributed in the 5 subfamilies( ABCA、ABCB、ABCC、ABCD、ABCE、ABCF、ABCG 和 ABCI). The evolutionary analysis of 168 differentially Unigenes annotated in the NR database shows that the ABCE,ABCF,ABCG families and ABCI11 were clustered to a group,the ABCB,ABCC families and ABCI10 were clustered to a group,and the ABCA,ABCD families and ABCI19 were clustered to a group. The information obtained from ABC transporters provides a theoretical basis for further study on drought resistance genes of ABC transporters in wild soybean.
In order to obtain the genes related to ABC transporter protein family under the conditions of drought stress,the drought-resistant wild soybean accession was used as experimental material which was treated with 20% PEG6000 solution to simulate drought stress. RNA was extracted from leaves of drought treated 0,6,12,24 and 48 h respectively,and transcriptome sequencing was carried out by Illumina Hi Seq 2000 platform. Based on the results of GO,KEGG and NR 3 major database notes,and then using ABC transporter as the key words to screen,a total of 337 ABC transporter related sequences were obtained,including 8 subfamilies( ABCA、ABCB、ABCC、ABCD、ABCE、ABCF、ABCG 和 ABCI). Differential expression analysis showed that there were 182 differentially expressed genes. Most of the differentially expressed genes annotated by GO database were purine ribonucleotide catabolic processes,while the Unigenes annotated by KEGG database were distributed in the 5 subfamilies( ABCA、ABCB、ABCC、ABCD、ABCE、ABCF、ABCG 和 ABCI). The evolutionary analysis of 168 differentially Unigenes annotated in the NR database shows that the ABCE,ABCF,ABCG families and ABCI11 were clustered to a group,the ABCB,ABCC families and ABCI10 were clustered to a group,and the ABCA,ABCD families and ABCI19 were clustered to a group. The information obtained from ABC transporters provides a theoretical basis for further study on drought resistance genes of ABC transporters in wild soybean.
引文
[1] Yuan C P,Wang Y J,Zhao H K,Zhang L,Wang Y M,Liu X D,Zhong X F,Dong Y S. Genetic diversity of rhg1 and Rhg4 loci in wild soybeans resistant to soybean cyst nematode race 3[J]. Genetics&Molecular Research,2016,15(2):1-10
[2] Kim M Y,Lee S,Van K,Kim T H,Jeong S C,ChoiⅠY,Kim D S,Lee Y S,Park D,Ma J,Kim W Y,Kim B C,Park S,Lee K A,Kim D H,Kim K H,Shin J H,Jang Y E,Kim K D,Liu W X,Chaisan T,Kang Y J,Lee Y H,Kim K H,Moon J K,Schmutz J,Jackson S A,Bhak J,Lee S H. Whole-genome sequencing and intensive analysis of the undomesticated soybean(Glycine soja Sieb.and Zucc.)genome[J]. Proceedings of the National Academy of Sciences of the United States of America,2010,107(51):22032-22037
[3] Lam H M,Xu X,Liu X,Chen W,Yang G,Wong F L,Li M W,He W,Qin N,Wang B,Li J,Jian M,Wang J,Shao G,Wang J,Sun S S,Zhang G. Resequencing of 31 wild and cultivated soybean genomes identifies patterns of genetic diversity and selection[J].Nature Genetics,2010,42(12):1053-1059
[4] Higgins C F. ABC transporters:From microorganisms to man[J].Annual Review of Plant Biology,1992,8(1):67-113
[5]曹冠华,柏旭,陈迪,张晓蓉,贺森.ABC转运蛋白结构特点及在植物和真菌重金属耐性中的作用与机制[J].农业生物技术学报,2016,24(10):1617-1628
[6] Beis K. Structural basis for the mechanism of ABC transporters[J].Biochemical Society Transactions,2015,43(5):889
[7] Broehan G,Kroeger T,Lorenzen M,Merzendorfer H. Functional analysis of the ATP-binding cassette(ABC)transporter gene family of Tribolium castaneum[J]. BMC Genomics,2013,14(1):6
[8]刘艳青,赵永芳.ABC转运蛋白结构与转运机制的研究进展[J].生命科学,2017,29(3):223-229
[9]曹冠华,柏旭,陈迪,张晓蓉,贺森.ABC转运蛋白结构特点及在植物和真菌重金属耐性中的作用与机制[J].农业生物技术学报,2016,24(10):1617-1628
[10]邵若玄,沈忆珂,周文彬,方佳,郑炳松.植物ATP结合盒(ABC)转运蛋白研究进展[J].浙江农林大学学报,2013,30(5):761-768
[11] Kang J,Pei Z M. PDR-type ABC transporter mediates cellular uptake of the phytohormone abscisic acid[J]. Proceedings of the National Academy of Sciences of the United States of America,2010,107(5):2355-2360
[12] Schulz B,Kolukisaoglu H U. Genomics of plant ABC transporters:the alphabet of photosynthetic life forms or just holes in membranes?[J]. Febs Letters,2006,580(4):1010-1016
[13]李依民,雷根平,颜永刚,彭亮,张娜,刘亮亮,黑小斌,李欢,张岗,郭顺星.铁皮石斛2个F家族ABC转运蛋白基因的克隆和表达研究[J].中草药,2017,48(15):3153-3159
[14]唐玉瑾.葡萄ABCG基因亚家族半分子成员克隆及表达分析[D].杨凌:西北农林科技大学,2016
[15]吴鹏,郭茜茜,武涛,秦智伟.黄瓜ABC转运蛋白基因(abca19)的克隆及其对农药霜霉威胁迫的响应[J].作物杂志,2015(3):45-51
[16] Rea P A. Plant ATP-binding cassette transporters[J]. Annual Review of Plant Biology,2007,58:347-375
[17] Kaneda M,Schuetz M,Lin B S,Chanis C,Hamberger B,Western T L, Ehlting J, Samuels A L. ABC transporters coordinately expressed during lignification of Arabidopsis stems include a set of ABCBs associated with auxin transport[J]. Journal of Experimental Botany,2011,62(6):2063
[18]卢金淼,李琴,李智平.表观遗传学因素对转运蛋白ABCB1的影响及其在药代动力学方面的作用[J].儿科药学杂志,2018,24(5):50-54
[19] Mcfarlane H E,Shin J J,Bird D A,Samuels A L. Arabidopsis ABCG transporters,which are required for export of diverse cuticular lipids,dimerize in different combinations[J]. Plant Cell,2010,22(9):3066-3075
[20]赵胡.阜豆GmABC基因的克隆及表达分析[J].西北植物学报,2012,32(9):1726-1730
[21]王玲玲.大豆ABC转运蛋白基因的克隆及响应铝和重金属胁迫的表达分析[C]//江苏省遗传学会第八届会员代表大会暨学术研讨会论文集.江苏:江苏省遗传学会,2010:90-91
[22]马进,郑钢.利用转录组测序技术鉴定紫花苜蓿根系盐胁迫应答基因[J].核农学报,2016,30(8):1470-1479
[23]李惠华,常强,王伟,曾碧玉,徐剑,苏明华.基于转录组测序的枇杷五环三萜合成途径差异基因的分析[J].农业生物技术学报,2018,26(2):222-233
[24]张少平,张少华,邱珊莲,张帅,吴松海,何炎森,郑开斌,郑加协.基于转录组测序的紫背天葵花青素相关基因分析[J].核农学报,2018,32(4):639-645
[25]王洋. Na HCO3模拟盐碱混合胁迫下野生大豆转录组研究[D].哈尔滨:东北农业大学,2014
[26] Zhang J, Wang J, Jiang W, Liu J, Yang S, Gai J, Li Y.Identification and analysis of Na HCO3stress responsive genes in wild Soybean(Glycine soja)roots by RNA-seq[J]. Frontiers in Plant Science,2016,7:1842
[27] Shi G,Huang F,Gong Y,Xu G,Yu J,Hu Z,Cai Q,Yu D. RNA-Seq analysis reveals that multiple phytohormone biosynthesis and signal transduction pathways are reprogrammed in curled-cotyledons mutant of soybean[Glycine max(L.)Merr.][J]. BMC Genomics,2014,15(1):510
[28] Yuan S,Rong L,Chen S,Chen H,Zhang C,Chen L,Hao Q,Shan Z,Yang Z,Qiu D,Zhang X,Zhou X. RNA-Seq analysis of differential gene expression responding to different rhizobium strains in Soybean(Glycine max)roots.[J]. Frontiers in Plant Science,2016,7(6):7363
[29] Duanmu H,Wang Y,Bai X,Cheng S,Deyholos M K,Wong G K,Li D,Zhu D,Li R,Yu Y,Cao L,Chen C,Zhu Y. Wild soybean roots depend on specific transcription factors and oxidation reduction related genesin response to alkaline stress[J]. Functional&Integrative Genomics,2015,15(6):651-660
[30]蒲伟凤.不同类型大豆种质资源抗旱性比较及野生大豆抗旱性筛选[D].秦皇岛:河北科技师范学院,2010
[31]张小芳,王冰冰,徐燕,张炜坤,赵恢,张锴,乔亚科,李桂兰.PEG模拟干旱胁迫下野生大豆转录组分析[J].大豆科学,2018,37(5):681-689
[32]朱璐,许杰,张大兵.拟南芥ABC转运类蛋白家族的分子进化、表达模式和蛋白功能网络预测分析[J].植物生理学报,2012,48(12):1151-1166
[33] Byrne S L,Durandeau K,Nagy I,Barth S. Identification of ABC transporters from Lolium perenne L. that are regulated by toxic levels of selenium[J]. Planta,2010,231(4):901-911
[34] Maathuis F J,FilatovⅤ,Herzyk P C,Krijger G B,Axelsen K,Chen S,Green BJ,Li Y,Madagan K L,Sanchez-Fernandez R,Forde B G,Palmgren M G,Rea P A,Williams L E,Sanders D,Amtmann A. Transcriptome analysis of root transporters reveals participation of multiple gene families in the response to cation stress[J]. Plant Journal,2003,35(6):675-692
[35] Wu G,Carville J S,Spalding E P. ABCB19-mediated polar auxin transport modulates Arabidopsis hypocotyl elongation and the endoreplication variant of the cell cycle[J]. Plant Journal,2016,85(2):209-218
[36] Lee M,Choi Y,Burla B,Kim Y Y,Jeon B,Maeshima M,Yoo J Y,Martinoia E,Lee Y. The ABC transporter At ABCB14 is a malate importer and modulates stomatal response to CO2[J]. Nature Cell Biology,2008,10(10):1217-1223
[37] Nagy R,Grob H,Weder B,Green P,Klein M,Frelet-Barrand A,Schjoerring J K,Brearley C,Martinoia E. The Arabidopsis ATPbinding cassette protein At MRP5/At ABCC5 is a high affinity inositol Hexakisphosphate transporter involved in guard Cell signaling and phytate storage[J]. Journal of Biological Chemistry,2009,284(48):33614-33622
[38] Galbiati M, Simoni L, Pavesi G, Cominelli E, Francia P,Vavasseur A,Nelson T, Bevan M, Tonelli C. Gene trap lines identify Arabidopsis genes expressed in stomatal guard cells[J].Plant Journal,2010,53(5):750-762
[39] Hafiane A,Genest J. ATP binding cassette A1(ABCA1)mediates microparticle formation during high-density lipoprotein(HDL)biogenesis[J]. Atherosclerosis,2017,257:90-99
[40]张利国.拟南芥OFP1与BLH3、ATH1蛋白互作与相关功能分析[D].哈尔滨:东北农业大学,2016
[41] Roeck A D,Duchateau L,Dongen JⅤ,Cacace R,Bjerke M,Van den Bossche T, Cras P, Vandenberghe R, De Deyn P P,Engelborghs S,Van Broeckhoven C,Sleegers K. An intronic VNTR affects splicing of ABCA7,and increases risk of Alzheimer’s disease[J]. Acta Neuropathologica,2018,135(6):1-11
[42]徐杏,邱杰,徐扬,徐辰武.水稻ABCB转运蛋白基因的分子进化和表达分析[J].中国水稻科学,2012,26(2):127-136
[43]张婧,陈梦词,马清,未丽,王锁民.植物ABCG转运蛋白研究进展[J].草业学报,2015,24(7):180-188
[44]王晓珠,孙万梅,马义峰,韩三琴,韩丽慧,王邦俊.拟南芥ABC转运蛋白研究进展[J].植物生理学报,2017,53(2):133-144
[45] Bussell J D,Reichelt M,Wiszniewski A A,Gershenzon J,Smith S M. Peroxisomal ATP-binding cassette transporter COMATOSE and the multifunctional protein abnormal INFLORESCENCE MERISTEM are required for the production of benzoylated metabolites in Arabidopsis seeds[J]. Plant Physiology,2014,164(1):48-54
[2] Kim M Y,Lee S,Van K,Kim T H,Jeong S C,ChoiⅠY,Kim D S,Lee Y S,Park D,Ma J,Kim W Y,Kim B C,Park S,Lee K A,Kim D H,Kim K H,Shin J H,Jang Y E,Kim K D,Liu W X,Chaisan T,Kang Y J,Lee Y H,Kim K H,Moon J K,Schmutz J,Jackson S A,Bhak J,Lee S H. Whole-genome sequencing and intensive analysis of the undomesticated soybean(Glycine soja Sieb.and Zucc.)genome[J]. Proceedings of the National Academy of Sciences of the United States of America,2010,107(51):22032-22037
[3] Lam H M,Xu X,Liu X,Chen W,Yang G,Wong F L,Li M W,He W,Qin N,Wang B,Li J,Jian M,Wang J,Shao G,Wang J,Sun S S,Zhang G. Resequencing of 31 wild and cultivated soybean genomes identifies patterns of genetic diversity and selection[J].Nature Genetics,2010,42(12):1053-1059
[4] Higgins C F. ABC transporters:From microorganisms to man[J].Annual Review of Plant Biology,1992,8(1):67-113
[5]曹冠华,柏旭,陈迪,张晓蓉,贺森.ABC转运蛋白结构特点及在植物和真菌重金属耐性中的作用与机制[J].农业生物技术学报,2016,24(10):1617-1628
[6] Beis K. Structural basis for the mechanism of ABC transporters[J].Biochemical Society Transactions,2015,43(5):889
[7] Broehan G,Kroeger T,Lorenzen M,Merzendorfer H. Functional analysis of the ATP-binding cassette(ABC)transporter gene family of Tribolium castaneum[J]. BMC Genomics,2013,14(1):6
[8]刘艳青,赵永芳.ABC转运蛋白结构与转运机制的研究进展[J].生命科学,2017,29(3):223-229
[9]曹冠华,柏旭,陈迪,张晓蓉,贺森.ABC转运蛋白结构特点及在植物和真菌重金属耐性中的作用与机制[J].农业生物技术学报,2016,24(10):1617-1628
[10]邵若玄,沈忆珂,周文彬,方佳,郑炳松.植物ATP结合盒(ABC)转运蛋白研究进展[J].浙江农林大学学报,2013,30(5):761-768
[11] Kang J,Pei Z M. PDR-type ABC transporter mediates cellular uptake of the phytohormone abscisic acid[J]. Proceedings of the National Academy of Sciences of the United States of America,2010,107(5):2355-2360
[12] Schulz B,Kolukisaoglu H U. Genomics of plant ABC transporters:the alphabet of photosynthetic life forms or just holes in membranes?[J]. Febs Letters,2006,580(4):1010-1016
[13]李依民,雷根平,颜永刚,彭亮,张娜,刘亮亮,黑小斌,李欢,张岗,郭顺星.铁皮石斛2个F家族ABC转运蛋白基因的克隆和表达研究[J].中草药,2017,48(15):3153-3159
[14]唐玉瑾.葡萄ABCG基因亚家族半分子成员克隆及表达分析[D].杨凌:西北农林科技大学,2016
[15]吴鹏,郭茜茜,武涛,秦智伟.黄瓜ABC转运蛋白基因(abca19)的克隆及其对农药霜霉威胁迫的响应[J].作物杂志,2015(3):45-51
[16] Rea P A. Plant ATP-binding cassette transporters[J]. Annual Review of Plant Biology,2007,58:347-375
[17] Kaneda M,Schuetz M,Lin B S,Chanis C,Hamberger B,Western T L, Ehlting J, Samuels A L. ABC transporters coordinately expressed during lignification of Arabidopsis stems include a set of ABCBs associated with auxin transport[J]. Journal of Experimental Botany,2011,62(6):2063
[18]卢金淼,李琴,李智平.表观遗传学因素对转运蛋白ABCB1的影响及其在药代动力学方面的作用[J].儿科药学杂志,2018,24(5):50-54
[19] Mcfarlane H E,Shin J J,Bird D A,Samuels A L. Arabidopsis ABCG transporters,which are required for export of diverse cuticular lipids,dimerize in different combinations[J]. Plant Cell,2010,22(9):3066-3075
[20]赵胡.阜豆GmABC基因的克隆及表达分析[J].西北植物学报,2012,32(9):1726-1730
[21]王玲玲.大豆ABC转运蛋白基因的克隆及响应铝和重金属胁迫的表达分析[C]//江苏省遗传学会第八届会员代表大会暨学术研讨会论文集.江苏:江苏省遗传学会,2010:90-91
[22]马进,郑钢.利用转录组测序技术鉴定紫花苜蓿根系盐胁迫应答基因[J].核农学报,2016,30(8):1470-1479
[23]李惠华,常强,王伟,曾碧玉,徐剑,苏明华.基于转录组测序的枇杷五环三萜合成途径差异基因的分析[J].农业生物技术学报,2018,26(2):222-233
[24]张少平,张少华,邱珊莲,张帅,吴松海,何炎森,郑开斌,郑加协.基于转录组测序的紫背天葵花青素相关基因分析[J].核农学报,2018,32(4):639-645
[25]王洋. Na HCO3模拟盐碱混合胁迫下野生大豆转录组研究[D].哈尔滨:东北农业大学,2014
[26] Zhang J, Wang J, Jiang W, Liu J, Yang S, Gai J, Li Y.Identification and analysis of Na HCO3stress responsive genes in wild Soybean(Glycine soja)roots by RNA-seq[J]. Frontiers in Plant Science,2016,7:1842
[27] Shi G,Huang F,Gong Y,Xu G,Yu J,Hu Z,Cai Q,Yu D. RNA-Seq analysis reveals that multiple phytohormone biosynthesis and signal transduction pathways are reprogrammed in curled-cotyledons mutant of soybean[Glycine max(L.)Merr.][J]. BMC Genomics,2014,15(1):510
[28] Yuan S,Rong L,Chen S,Chen H,Zhang C,Chen L,Hao Q,Shan Z,Yang Z,Qiu D,Zhang X,Zhou X. RNA-Seq analysis of differential gene expression responding to different rhizobium strains in Soybean(Glycine max)roots.[J]. Frontiers in Plant Science,2016,7(6):7363
[29] Duanmu H,Wang Y,Bai X,Cheng S,Deyholos M K,Wong G K,Li D,Zhu D,Li R,Yu Y,Cao L,Chen C,Zhu Y. Wild soybean roots depend on specific transcription factors and oxidation reduction related genesin response to alkaline stress[J]. Functional&Integrative Genomics,2015,15(6):651-660
[30]蒲伟凤.不同类型大豆种质资源抗旱性比较及野生大豆抗旱性筛选[D].秦皇岛:河北科技师范学院,2010
[31]张小芳,王冰冰,徐燕,张炜坤,赵恢,张锴,乔亚科,李桂兰.PEG模拟干旱胁迫下野生大豆转录组分析[J].大豆科学,2018,37(5):681-689
[32]朱璐,许杰,张大兵.拟南芥ABC转运类蛋白家族的分子进化、表达模式和蛋白功能网络预测分析[J].植物生理学报,2012,48(12):1151-1166
[33] Byrne S L,Durandeau K,Nagy I,Barth S. Identification of ABC transporters from Lolium perenne L. that are regulated by toxic levels of selenium[J]. Planta,2010,231(4):901-911
[34] Maathuis F J,FilatovⅤ,Herzyk P C,Krijger G B,Axelsen K,Chen S,Green BJ,Li Y,Madagan K L,Sanchez-Fernandez R,Forde B G,Palmgren M G,Rea P A,Williams L E,Sanders D,Amtmann A. Transcriptome analysis of root transporters reveals participation of multiple gene families in the response to cation stress[J]. Plant Journal,2003,35(6):675-692
[35] Wu G,Carville J S,Spalding E P. ABCB19-mediated polar auxin transport modulates Arabidopsis hypocotyl elongation and the endoreplication variant of the cell cycle[J]. Plant Journal,2016,85(2):209-218
[36] Lee M,Choi Y,Burla B,Kim Y Y,Jeon B,Maeshima M,Yoo J Y,Martinoia E,Lee Y. The ABC transporter At ABCB14 is a malate importer and modulates stomatal response to CO2[J]. Nature Cell Biology,2008,10(10):1217-1223
[37] Nagy R,Grob H,Weder B,Green P,Klein M,Frelet-Barrand A,Schjoerring J K,Brearley C,Martinoia E. The Arabidopsis ATPbinding cassette protein At MRP5/At ABCC5 is a high affinity inositol Hexakisphosphate transporter involved in guard Cell signaling and phytate storage[J]. Journal of Biological Chemistry,2009,284(48):33614-33622
[38] Galbiati M, Simoni L, Pavesi G, Cominelli E, Francia P,Vavasseur A,Nelson T, Bevan M, Tonelli C. Gene trap lines identify Arabidopsis genes expressed in stomatal guard cells[J].Plant Journal,2010,53(5):750-762
[39] Hafiane A,Genest J. ATP binding cassette A1(ABCA1)mediates microparticle formation during high-density lipoprotein(HDL)biogenesis[J]. Atherosclerosis,2017,257:90-99
[40]张利国.拟南芥OFP1与BLH3、ATH1蛋白互作与相关功能分析[D].哈尔滨:东北农业大学,2016
[41] Roeck A D,Duchateau L,Dongen JⅤ,Cacace R,Bjerke M,Van den Bossche T, Cras P, Vandenberghe R, De Deyn P P,Engelborghs S,Van Broeckhoven C,Sleegers K. An intronic VNTR affects splicing of ABCA7,and increases risk of Alzheimer’s disease[J]. Acta Neuropathologica,2018,135(6):1-11
[42]徐杏,邱杰,徐扬,徐辰武.水稻ABCB转运蛋白基因的分子进化和表达分析[J].中国水稻科学,2012,26(2):127-136
[43]张婧,陈梦词,马清,未丽,王锁民.植物ABCG转运蛋白研究进展[J].草业学报,2015,24(7):180-188
[44]王晓珠,孙万梅,马义峰,韩三琴,韩丽慧,王邦俊.拟南芥ABC转运蛋白研究进展[J].植物生理学报,2017,53(2):133-144
[45] Bussell J D,Reichelt M,Wiszniewski A A,Gershenzon J,Smith S M. Peroxisomal ATP-binding cassette transporter COMATOSE and the multifunctional protein abnormal INFLORESCENCE MERISTEM are required for the production of benzoylated metabolites in Arabidopsis seeds[J]. Plant Physiology,2014,164(1):48-54
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