白囊耙齿菌对茜素红脱色条件优化及其毒性变化检测
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摘要
白囊耙齿菌Irpexlacteus是分离自倒木上的一株可以分泌漆酶和锰过氧化物酶的白腐真菌。利用I. lacteus对固体条件下的活性黑、活性红、结晶紫、茜素红和孔雀石绿进行脱色能力的检测,通过单因素和正交试验优化I. lacteus对茜素红的脱色条件,并以3种作物发芽率为指标测定茜素红被I. lacteus脱色前后的毒性变化。结果显示,I.lacteus对5种染料均可脱色,其中对茜素红染料的脱色更为彻底;单因素和正交试验优化I. lacteus对茜素红的脱色条件为:pH 7.0、葡萄糖10.0g/L、硫酸铵0.66g/L、接种量2片(Φ=8.0mm)、100.0mL三角瓶装液20.0mL,优化条件下I.lacteus对茜素红脱色10d时的脱色率为88.26%,与未优化前的脱色率相比提高了60.50%;茜素红染料被I. lacteus脱色前后毒性大小排序为:染料原液>染料脱色后>PDB培养基处理,表明茜素红染料存在一定的毒性,I.lacteus脱色茜素红后可以使其毒性减弱。通过本研究,为I. lacteus在茜素红等染料废水脱色以及降低染料废水毒性方面的应用奠定基础。
Irpex lacteus, a white rot fungus isolated from fallen wood, which can secrete laccase and manganese peroxidase, was tested for its ability to decolorize reactive black, reactive red, crystal violet,alizarin red and malachite green under solid conditions. The decolorizing conditions of alizarin red by I. lacteus were optimized by single factor and orthogonal test, and the toxicity changes of alizarin red before and after decolorization were measured with germination rate of three crops as indicators. The results showed that I.lacteus could decolorize all of the five direct dyes, especially alizarin red. The optimized conditions for alizarin red decolorization included medium pH value of 7.0, 20.0 g/L of glucose, 0.66 g/L of ammonium sulfate, and two pieces of inoculum cake(diameter 8.0 mm) in 20.0 mL alizarin red solution. Under such optimized conditions, the decolorization rate of alizarin red was 88.26% in 10 d, 60.50% increase as compared with the decolorization rate before optimization. The toxicity of alizarin red dye before and after decolorization decreased progressively in order of dye stock solution > decolorized solution > solution treated with PDB medium. Alizarin red dye has certain toxicity, but I. lacteus can reduce its toxicity during decolorization. This study provides reference for application of I. lacteus in decolorization of dye wastewater such as alizarin red and reduction of toxicity of dye wastewater.
Irpex lacteus, a white rot fungus isolated from fallen wood, which can secrete laccase and manganese peroxidase, was tested for its ability to decolorize reactive black, reactive red, crystal violet,alizarin red and malachite green under solid conditions. The decolorizing conditions of alizarin red by I. lacteus were optimized by single factor and orthogonal test, and the toxicity changes of alizarin red before and after decolorization were measured with germination rate of three crops as indicators. The results showed that I.lacteus could decolorize all of the five direct dyes, especially alizarin red. The optimized conditions for alizarin red decolorization included medium pH value of 7.0, 20.0 g/L of glucose, 0.66 g/L of ammonium sulfate, and two pieces of inoculum cake(diameter 8.0 mm) in 20.0 mL alizarin red solution. Under such optimized conditions, the decolorization rate of alizarin red was 88.26% in 10 d, 60.50% increase as compared with the decolorization rate before optimization. The toxicity of alizarin red dye before and after decolorization decreased progressively in order of dye stock solution > decolorized solution > solution treated with PDB medium. Alizarin red dye has certain toxicity, but I. lacteus can reduce its toxicity during decolorization. This study provides reference for application of I. lacteus in decolorization of dye wastewater such as alizarin red and reduction of toxicity of dye wastewater.
引文
Chen L,Lin YH,He XB,Tan L,Tang X,Hu YT,2011.Optimization of decolorization of aniline blue dye by white-rot fungus Bjerkandera fumosa XX-3.Hubei Agricultural Sciences,50(20):4178-4180+4184(in Chinese)
Chen WH,Li G,Xu FC,Pan X,Wen LN,Du LN,2014.Research progress on pollution and treatment of dye wastewater.Journal of Zhejiang Agricultural Sciences,1(2):264-269(in Chinese)
Cheng YQ,Huang MS,Zhang GY,2000.Decoloration and degradation of dyes by white rot fungi.Techniques and Equipment for Environmental Pollution Control,6:25-34(in Chinese)
Ding SL,Li ZK,Wang R,2010.Summary of treatment of dyestuff wastewater.Water Resources Protection,3:73-78(in Chinese)
Guo LX,Ruan HX,Chen WD,2016.Study on the technology of dye wastewater treatment.Chemical Management,1(19):212-213(in Chinese)
Han QC,Huo GH,Luo GX,2014.Three species of wild macrofungi with decolorization of malachite green and their decolorization conditions.Mycosystema,33(4):905-915(in Chinese)
Li N,Li J,Lu L,Wang JY,Wang TN,Li GF,Xu TF,Zhao M,2014.Application of immobilized laccase in dye decolorization.Journal of Nanjing Forestry University(Natural Sciences Edition),38(5):129-133(in Chinese)
Liang B,Xu JQ,Guan J,Guo YG,Su RJ,2015.Research progresses in treatment of dyeing waste water by biological methods.Environmental Protection of Chemical Industry,35(3):259-266(in Chinese)
Liang H,2008.Optimization of incubation conditions of Phanerochaete chrysosporium and its decolorization performance of azo dye.Master Thesis,Northeast Forestry University,Harbin.1-50(in Chinese)
Liang YY,Zhang CH,Zhou XY,2003.Progress in reseach on utilization of Phanerochaete chrysosporium for industial wastewater treatment.Technology of Water Treatment,29(5):253-257(in Chinese)
Luo X,Qin YX,Yu C,2018.Optimization of Irpex lacteus culture conditions for manganese peroxidase production and dye decolorization ability of the enzyme.Mycosystema,37(9):1233-1242(in Chinese)
Lv P,2016.The development of microbial degradation of textile dyes.Value Engineering,35(18):217-221(in Chinese)
Qi C,2016.Screening,identification and decolorization conditions and mechanisms of isolated strain for the degradation of azo dye reactive black 5.Master Thesis,Nanjing Agricultural University,Nanjing.1-59(in Chinese)
Qian C,2005.Cloning of laccase gene from Irpex lacteus and correlated sequence analysis.Master Thesis,Northeast Forestry University,Harbin.1-54(in Chinese)
Si J,Cui BK,Dai YC,2011.Application in dye decolorization and optimization of conditions in discoloration by Trametes orientalis.Genomics and Applied Biology,30(3):364-371(in Chinese)
Si J,Cui BK,Li MJ,2012.Dye decolorization and adsorptive mechanism by the adsorbents obtained from Trametes orientalis biomass.Mycosystema,31(6):890-899(in Chinese)
Yang ZH,2010.Research on the comparison of different methods for the advanced treatment of printing and dyeing wastewater.Industrial Water Treatment,30(7):42-45(in Chinese)
Yu C,Chi YJ,2014.Decolorization of reactive dyes by Cerrena unicolor.Journal of Beijing Forestry University,36(4):126-132(in Chinese)
Yuan HS,Dai YC,Cao Y,Yang J,2010.Screening of white-rot fungi for decolorizing synthetic dyes and investigation on factors affecting decolorizing capacity of Cerrena unicolor.Mycosystema,29(3):429-436(in Chinese)
Zhou MY,1992.Environmental organic pollutants and carcinogens.Sichuan University Press,Chengdu.1-228(in Chinese)
陈亮,林永慧,何兴兵,谭玲,唐笑,胡玉婷,2011.白腐真菌XX-3对苯胺蓝染料的脱色条件优化.湖北农业科学,50(20):4178-4180+4184
陈文华,李刚,许方程,泮琇,温玲宁,都林娜,2014.染料废水污染现状及处理方法研究进展.浙江农业科学,1(2):264-269
程永前,黄民生,张国莹,2000.白腐真菌对染料脱色及降解过程机理和影响因素.环境污染治理技术与设备,6:25-34
丁绍兰,李郑坤,王睿,2010.染料废水处理技术综述.水资源保护,3:73-78
郭理想,阮海兴,陈伟东,2016.染料废水处理技术方法的研究.化工管理,1(19):212-213
韩启灿,霍光华,罗桂祥,2014.三株野生大型真菌对孔雀石绿的脱色作用及其脱色条件.菌物学报,33(4):905-915
历娜,栗君,卢磊,王靖瑶,王天女,李国富,徐腾飞,赵敏,2014.固定化漆酶在染料脱色中的应用.南京林业大学学报(自然科学版),38(5):129-133
梁波,徐金球,关杰,郭耀广,苏瑞景,2015.生物法处理印染废水的研究进展.化工环保,35(3):259-266
梁红,2008.黄孢原毛平革菌培养条件的优化及对染料的降解研究.东北林业大学硕士论文,哈尔滨.1-50
梁园园,张朝辉,周晓云,2003.应用黄孢原毛平革菌处理工业废水的研究进展.水处理技术,29(5):253-257
罗鑫,覃育贤,于存,2018.白囊耙齿菌产锰过氧化物酶条件优化及其对染料的脱色.菌物学报,37(9):1233-1242
吕鹏,2016.微生物降解纺织染料研究进展.价值工程,35(18):217-221
齐澄,2016.一株偶氮染料活性黑5降解菌的筛选、鉴定与其脱色条件及机理研究.南京农业大学硕士论文,南京.1-59
钱程,2005.白囊耙齿菌(Irpex lacteus)漆酶基因的克隆及相关序列的分析.东北林业大学硕士论文,哈尔滨.1-54
司静,崔宝凯,戴玉成,2011.东方栓孔菌在染料脱色中的应用及其脱色条件的优化.基因组学与应用生物学,30(3):364-371
司静,崔宝凯,李牧洁,2012.东方栓孔菌菌丝体生物吸附剂对染料的脱色作用及其吸附机制研究.菌物学报,31(6):890-899
杨占红,2010.不同方法深度处理印染废水的比较研究.工业水处理,30(7):42-45
于存,池玉杰,2014.一色齿毛菌对活性染料的脱色研究.北京林业大学学报,36(4):126-132
袁海生,戴玉成,曹云,杨建,2010.白腐真菌染料脱色菌株的筛选及一色齿毛菌脱色条件的研究.菌物学报,29(3):429-436
周明耀,1992.环境有机污染物与致癌物质.成都:四川大学出版社.1-228
Chen WH,Li G,Xu FC,Pan X,Wen LN,Du LN,2014.Research progress on pollution and treatment of dye wastewater.Journal of Zhejiang Agricultural Sciences,1(2):264-269(in Chinese)
Cheng YQ,Huang MS,Zhang GY,2000.Decoloration and degradation of dyes by white rot fungi.Techniques and Equipment for Environmental Pollution Control,6:25-34(in Chinese)
Ding SL,Li ZK,Wang R,2010.Summary of treatment of dyestuff wastewater.Water Resources Protection,3:73-78(in Chinese)
Guo LX,Ruan HX,Chen WD,2016.Study on the technology of dye wastewater treatment.Chemical Management,1(19):212-213(in Chinese)
Han QC,Huo GH,Luo GX,2014.Three species of wild macrofungi with decolorization of malachite green and their decolorization conditions.Mycosystema,33(4):905-915(in Chinese)
Li N,Li J,Lu L,Wang JY,Wang TN,Li GF,Xu TF,Zhao M,2014.Application of immobilized laccase in dye decolorization.Journal of Nanjing Forestry University(Natural Sciences Edition),38(5):129-133(in Chinese)
Liang B,Xu JQ,Guan J,Guo YG,Su RJ,2015.Research progresses in treatment of dyeing waste water by biological methods.Environmental Protection of Chemical Industry,35(3):259-266(in Chinese)
Liang H,2008.Optimization of incubation conditions of Phanerochaete chrysosporium and its decolorization performance of azo dye.Master Thesis,Northeast Forestry University,Harbin.1-50(in Chinese)
Liang YY,Zhang CH,Zhou XY,2003.Progress in reseach on utilization of Phanerochaete chrysosporium for industial wastewater treatment.Technology of Water Treatment,29(5):253-257(in Chinese)
Luo X,Qin YX,Yu C,2018.Optimization of Irpex lacteus culture conditions for manganese peroxidase production and dye decolorization ability of the enzyme.Mycosystema,37(9):1233-1242(in Chinese)
Lv P,2016.The development of microbial degradation of textile dyes.Value Engineering,35(18):217-221(in Chinese)
Qi C,2016.Screening,identification and decolorization conditions and mechanisms of isolated strain for the degradation of azo dye reactive black 5.Master Thesis,Nanjing Agricultural University,Nanjing.1-59(in Chinese)
Qian C,2005.Cloning of laccase gene from Irpex lacteus and correlated sequence analysis.Master Thesis,Northeast Forestry University,Harbin.1-54(in Chinese)
Si J,Cui BK,Dai YC,2011.Application in dye decolorization and optimization of conditions in discoloration by Trametes orientalis.Genomics and Applied Biology,30(3):364-371(in Chinese)
Si J,Cui BK,Li MJ,2012.Dye decolorization and adsorptive mechanism by the adsorbents obtained from Trametes orientalis biomass.Mycosystema,31(6):890-899(in Chinese)
Yang ZH,2010.Research on the comparison of different methods for the advanced treatment of printing and dyeing wastewater.Industrial Water Treatment,30(7):42-45(in Chinese)
Yu C,Chi YJ,2014.Decolorization of reactive dyes by Cerrena unicolor.Journal of Beijing Forestry University,36(4):126-132(in Chinese)
Yuan HS,Dai YC,Cao Y,Yang J,2010.Screening of white-rot fungi for decolorizing synthetic dyes and investigation on factors affecting decolorizing capacity of Cerrena unicolor.Mycosystema,29(3):429-436(in Chinese)
Zhou MY,1992.Environmental organic pollutants and carcinogens.Sichuan University Press,Chengdu.1-228(in Chinese)
陈亮,林永慧,何兴兵,谭玲,唐笑,胡玉婷,2011.白腐真菌XX-3对苯胺蓝染料的脱色条件优化.湖北农业科学,50(20):4178-4180+4184
陈文华,李刚,许方程,泮琇,温玲宁,都林娜,2014.染料废水污染现状及处理方法研究进展.浙江农业科学,1(2):264-269
程永前,黄民生,张国莹,2000.白腐真菌对染料脱色及降解过程机理和影响因素.环境污染治理技术与设备,6:25-34
丁绍兰,李郑坤,王睿,2010.染料废水处理技术综述.水资源保护,3:73-78
郭理想,阮海兴,陈伟东,2016.染料废水处理技术方法的研究.化工管理,1(19):212-213
韩启灿,霍光华,罗桂祥,2014.三株野生大型真菌对孔雀石绿的脱色作用及其脱色条件.菌物学报,33(4):905-915
历娜,栗君,卢磊,王靖瑶,王天女,李国富,徐腾飞,赵敏,2014.固定化漆酶在染料脱色中的应用.南京林业大学学报(自然科学版),38(5):129-133
梁波,徐金球,关杰,郭耀广,苏瑞景,2015.生物法处理印染废水的研究进展.化工环保,35(3):259-266
梁红,2008.黄孢原毛平革菌培养条件的优化及对染料的降解研究.东北林业大学硕士论文,哈尔滨.1-50
梁园园,张朝辉,周晓云,2003.应用黄孢原毛平革菌处理工业废水的研究进展.水处理技术,29(5):253-257
罗鑫,覃育贤,于存,2018.白囊耙齿菌产锰过氧化物酶条件优化及其对染料的脱色.菌物学报,37(9):1233-1242
吕鹏,2016.微生物降解纺织染料研究进展.价值工程,35(18):217-221
齐澄,2016.一株偶氮染料活性黑5降解菌的筛选、鉴定与其脱色条件及机理研究.南京农业大学硕士论文,南京.1-59
钱程,2005.白囊耙齿菌(Irpex lacteus)漆酶基因的克隆及相关序列的分析.东北林业大学硕士论文,哈尔滨.1-54
司静,崔宝凯,戴玉成,2011.东方栓孔菌在染料脱色中的应用及其脱色条件的优化.基因组学与应用生物学,30(3):364-371
司静,崔宝凯,李牧洁,2012.东方栓孔菌菌丝体生物吸附剂对染料的脱色作用及其吸附机制研究.菌物学报,31(6):890-899
杨占红,2010.不同方法深度处理印染废水的比较研究.工业水处理,30(7):42-45
于存,池玉杰,2014.一色齿毛菌对活性染料的脱色研究.北京林业大学学报,36(4):126-132
袁海生,戴玉成,曹云,杨建,2010.白腐真菌染料脱色菌株的筛选及一色齿毛菌脱色条件的研究.菌物学报,29(3):429-436
周明耀,1992.环境有机污染物与致癌物质.成都:四川大学出版社.1-228
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