敲低钙调磷酸酶结合蛋白1引起肾小管上皮细胞线粒体损伤

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英文篇名：Knocking down Cabin1 induces renal tubular epithelial cell mitochondrial dysfunction 作者：吴汪丽 ; 温跃强 英文作者：WU Wangli;WEN Yueqiang;The Second Affiliated Hospital of Guangzhou Medical University; 关键词：肾小管上皮细胞 ; 线粒体损伤 ; 钙调磷酸酶结合蛋白1 英文关键词：Renal tubular epithelial cell;;Mitochondrial dysfunction;;Calcineurin binding protein 1 中文刊名：GZYY 英文刊名：Guangzhou Medical Journal 机构：广州医科大学附属第二医院; 出版日期：2019-05-20 出版单位：广州医药 年：2019 期：v.50 语种：中文; 页：GZYY201903003 页数：3 CN：03 ISSN：44-1199/R 分类号：15-17

摘要

目的探讨钙调磷酸酶结合蛋白1(calcineurin binding protein 1, Cabin1)在肾小管上皮细胞(renal tubular epithelial cells,RTECs)线粒体损伤中的作用机制。方法采用siRNA干预体外培养RTECs,敲低Cabin1的表达,继而以电镜观察其对RTECs线粒体形态的影响。结果在对照组和阴性对照组中Cabin1蛋白在RTECs中有高表达,采用siRNA干预RTECs后,Cabin1蛋白的表达量较对照组和阴性对照组降低50%以上(P<0.05)。对照组与阴性对照组中,线粒体形态规则,呈圆形或椭圆形,线粒体膜完整,线粒体嵴清晰可见。敲低组中,线粒体肿胀,呈长条形或不规则形,线粒体膜、线粒体嵴结构模糊甚至消失。结论敲低Cabin1引起RTECs的线粒体形态学异常,提示Cabin1是维持RTECs线粒体正常功能的重要分子。
        Objective To investigate the role of calcineurin binding protein 1(Cabin1) in renal tubular epithelial cells(RTECs) mitochondrial dysfunction. Methods Knocked down Cabin1 in RTECs with siRNA, Western bolt was applied to detect the level of Cabin1 protein. The morphology of mitochondria in RTECs were observed under microscopy. Results In control and negative control groups, Cabin1 protein was obviously expressed in RTECs. After knocked down by siRNA, Cabin1 protein expression was decreased(P<0.05). In Cabin1 knocked down group, mitochondria changed from large and ellipsoid shape to the small, long and irregulars. Morover, mitochondria were swollen and cristae were remarkably dissolved. Conclusion Knocked down Cabin1 induced RTECs mitochondrial dysfunction, which indicates Cabin1 is a crucial factor regulating mitochondrial function.

引文

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