黄芪茎叶对糖尿病大鼠认知障碍的影响
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摘要
目的:研究黄芪茎叶对链脲佐菌素(STZ)诱导的糖尿病大鼠所造成的认知障碍的影响。方法:一次性腹腔注射链脲佐菌素(60 mg/kg)诱导糖尿病大鼠模型。将造模成功的大鼠随机分为模型组、黄芪茎叶高、中、低剂量组(24 g/kg、12 g/kg、6 g/kg)、二甲双胍组(150 mg/kg)、黄芪组(6 g/kg)和正常组,每组8只,每天灌胃1次,连续7 d。7 d后进行Morris水迷宫实验,并检测空腹血糖和胸腺、脾脏指数。结果:在Morris水迷宫实验中,黄芪茎叶不同剂量组较模型组逃避潜伏期缩短,且撤出平台后,平台进入次数和有效区域进入次数明显增加,在平台及有效区域的运动距离增加。同时黄芪茎叶组糖尿病大鼠血糖水平显著降低(P<0.01),胸腺脏器指数显著提高(P<0.01,P<0.05)。结论:黄芪茎叶能有效降低糖尿病大鼠的血糖,提高其免疫力,显著改善STZ诱导的糖尿病大鼠的认知功能障碍。
Objective:To study the effects of stem and leaf of astragalus on cognitive impairment of diabetic rats induced by streptozotocin(STZ). Methods:Diabetic models were induced by intraperitoneal injection of streptozotocin(60 mg/kg).The rats were randomly divided into model group,high,medium and low stem and leaf of astragalus group(24 g/kg,12 g/kg,6 g/kg),metformingroup group(150 mg/kg),astragalus group(6 g/kg)and normal group,8 rats in each group. The rats in treatment groups were administered intragastically corresponding drugs respectively,1 time a day and continuous 7 d. 7 days later,Morris water maze test was performed and fasting blood glucose was detected and indices of thymus and spleen were calculated. Results:In the test of Morris water maze,the escape latent period of stem and leaf of astragalus groups was shorter than that of model group. After withdrawing from the platform,the numbers of platform entries and the numbers of effective area entries increased significantly in stem and leaf of astragalus groups,while the moving distances in platform and the effective areas were increased. At the same time,the blood sugar level of rats was significantly decreased(P<0.01),but the thymus index was increased significantly in stem and leaf of astragalus group(P<0.01,P<0.05). Conclusion:The stem and leaf of astragalus can effectively reduce the blood sugar of diabetic rats,improve their immunity and improve the cognitive impairment of diabetic rats induced by STZ.
Objective:To study the effects of stem and leaf of astragalus on cognitive impairment of diabetic rats induced by streptozotocin(STZ). Methods:Diabetic models were induced by intraperitoneal injection of streptozotocin(60 mg/kg).The rats were randomly divided into model group,high,medium and low stem and leaf of astragalus group(24 g/kg,12 g/kg,6 g/kg),metformingroup group(150 mg/kg),astragalus group(6 g/kg)and normal group,8 rats in each group. The rats in treatment groups were administered intragastically corresponding drugs respectively,1 time a day and continuous 7 d. 7 days later,Morris water maze test was performed and fasting blood glucose was detected and indices of thymus and spleen were calculated. Results:In the test of Morris water maze,the escape latent period of stem and leaf of astragalus groups was shorter than that of model group. After withdrawing from the platform,the numbers of platform entries and the numbers of effective area entries increased significantly in stem and leaf of astragalus groups,while the moving distances in platform and the effective areas were increased. At the same time,the blood sugar level of rats was significantly decreased(P<0.01),but the thymus index was increased significantly in stem and leaf of astragalus group(P<0.01,P<0.05). Conclusion:The stem and leaf of astragalus can effectively reduce the blood sugar of diabetic rats,improve their immunity and improve the cognitive impairment of diabetic rats induced by STZ.
引文
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[15]施怀生,毕小凤,史宪海. UPLC-Q-Exactive四极杆-静电场轨道阱高分辨质谱联用分析黄芪根及其茎叶中黄酮和皂苷类成分[J].世界中西医结合杂志,2018,13(3):357-361.
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[2]颜文慧,张纯茜,邢通,等.尼克地尔可改善链脲佐菌素诱导的糖尿病小鼠的认知功能障碍[J].南方医科大学学报,2018,38(4):384-389.
[3]侯软玲,董献红,李新强,等.银杏叶提取物对糖尿病学习记忆障碍的影响[J].新乡医学院学报,2010,27(3):250-252.
[4]王平,梁逸曾. HPLC-DAD-MS研究黄芪的化学成分[J].中草药,2011,42(2):226-229.
[5]马振平.黄芪叶化学成分及含量测定研究[D].哈尔滨:黑龙江中医药大学,2014.
[6]陈虎虎,龚苏晓,张铁军,等.黄芪茎、叶的化学成分和药理作用研究进展[J].药物评价研究,2011,34(2):134-137.
[7]靳晓飞,高维娟.黄芪甲苷生物活性及神经保护作用研究进展[J].河北中医药学报,2017,32(3):54-57.
[8]公惠玲,李卫平,尹艳艳,等.黄精多糖对链脲菌素糖尿病大鼠降血糖作用及其机制探讨[J].中国中药杂志,2009,39(9):1149-1154.
[9]金莉.不同剂量黄芪对糖尿病大鼠认知功能的影响机制研究[D].济南:山东中医药大学,2012.
[10]刘明,闫娟娟,周小江,等.开心散对慢性应激抑郁模型大鼠学习记忆的影响[J].中国中药杂志,2012,37(16):2439-2443.
[11]陈士玉.补脾益气中药对脾气虚痴呆大鼠水迷宫实验、白介素1、白介素6影响随机平行对照研究[J].实用中医内科杂志,2016,30(2):73-76.
[12]李晓冰,任玉梅,陈玉龙,等.当归芍药散对糖尿病大鼠认知障碍及海马组织病变的影响[J].时珍国医国药,2016,27(2):269-271.
[13]万朋,王师,郎茂林,等.黄芪对阿尔兹海默病模型大鼠学习记忆能力的影响[J].吉林医药学院学报,2011,32(2):69-73.
[14]李娜,李慧丽,刘德山.黄芪多糖对糖尿病大鼠认知功能障碍的改善作用.[J].中国老年学杂志,2017,37(5):2098-2099.
[15]施怀生,毕小凤,史宪海. UPLC-Q-Exactive四极杆-静电场轨道阱高分辨质谱联用分析黄芪根及其茎叶中黄酮和皂苷类成分[J].世界中西医结合杂志,2018,13(3):357-361.
[16]毕小凤,庞静,史宪海.蒙古黄芪根部与茎叶中部分药理活性成分差异研究[J].山西中医学院学报,2017,18(1):29-31.
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