原发性肝细胞癌中NOP14的表达及其临床意义
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摘要
目的探讨NOP14在肝细胞癌(hepatocellular carcinoma,HCC)组织中的表达及临床意义。方法检测97例HCC石蜡包埋组织及其癌旁组织中NOP14的表达,分析NOP14表达与HCC临床病理特征的关系。利用实时荧光定量PCR(real-time quantitative PCR,qRT-PCR)及Western blot法分别检测20对配对新鲜冻存HCC及癌旁组织中NOP14的表达。结果 NOP14在HCC组织中的高表达率为64. 9%(63/97),癌旁组织中的高表达率为12. 4%(12/97),差异有统计学意义(P <0. 001),NOP14在HCC组织中的高表达与肿瘤血管侵犯(P=0. 004)、TNM分期(P=0. 013)及Edmondson分级(P=0. 009)差异有统计学意义。qRT-PCR及Western blot法检测均显示HCC组织中NOP14的表达量显著高于配对癌旁组织,差异有统计学意义(P <0. 001,P <0. 001)。Kaplan-Meier生存分析和Log-rank检验显示:NOP14高表达与低表达患者的中位生存期分别为17、35个月,差异有统计学意义(P <0. 05)。结论 NOP14的表达与HCC是否存在血管侵犯、TNM分期以及Edmondson分级均有显著相关性,检测NOP14有助于HCC患者病情评估。
Purpose To investigate the expression and clinical significance of NOP14 in hepatocellular carcinoma( HCC). Methods The expression of NOP14 in HCC paraffinembedded tissues and adjacent tissues was confirmed in 97 cases. The relationship between NOP14 expression and clinicopathological features of HCC was analyzed. Real-time quantitative PCR( qRT-PCR) and Western blot were used to detect the expression of NOP14 in 20 pairs of freshly frozen HCC and adjacent tissues. Results The high expression rate of NOP14 in HCC tissues was 64. 9%( 63/97),while the high expression rate in adjacent tissues was 12. 4%( 12/97). The difference in expression between the two groups was statistically significant( P< 0. 001). The high expression of NOP14 in HCC tissues was significantly correlated with tumor vascular invasion( P =0. 004),TNM stage( P = 0. 013),and Edmondson classifica-tion( P = 0. 009). Both qRT-PCR and Western blot showed that the expression of NOP14 in HCC tissues was significantly higher than that in the matched adjacent tissues,and the difference between the groups was statistically significant( P < 0. 001,P <0. 001). Kaplan-Meier survival analysis and Log-rank test indicated that the median survival of patients with high and low NOP14 expression was 17 months and 35 months,respectively,and the difference was statistically significant( P < 0. 05). Conclusion The expression of NOP14 was significantly associated with the presence of HCC in vascular invasion,TNM staging,and Edmondson grading. The detection of NOP14 contributes to the assessment of the severity of HCC patients.
Purpose To investigate the expression and clinical significance of NOP14 in hepatocellular carcinoma( HCC). Methods The expression of NOP14 in HCC paraffinembedded tissues and adjacent tissues was confirmed in 97 cases. The relationship between NOP14 expression and clinicopathological features of HCC was analyzed. Real-time quantitative PCR( qRT-PCR) and Western blot were used to detect the expression of NOP14 in 20 pairs of freshly frozen HCC and adjacent tissues. Results The high expression rate of NOP14 in HCC tissues was 64. 9%( 63/97),while the high expression rate in adjacent tissues was 12. 4%( 12/97). The difference in expression between the two groups was statistically significant( P< 0. 001). The high expression of NOP14 in HCC tissues was significantly correlated with tumor vascular invasion( P =0. 004),TNM stage( P = 0. 013),and Edmondson classifica-tion( P = 0. 009). Both qRT-PCR and Western blot showed that the expression of NOP14 in HCC tissues was significantly higher than that in the matched adjacent tissues,and the difference between the groups was statistically significant( P < 0. 001,P <0. 001). Kaplan-Meier survival analysis and Log-rank test indicated that the median survival of patients with high and low NOP14 expression was 17 months and 35 months,respectively,and the difference was statistically significant( P < 0. 05). Conclusion The expression of NOP14 was significantly associated with the presence of HCC in vascular invasion,TNM staging,and Edmondson grading. The detection of NOP14 contributes to the assessment of the severity of HCC patients.
引文
[1]Torre L A,Siegel R L,Ward E M,et al.Global cancer incidenceand mortality rates and trends-an update[J].Cancer Epidemiol Biomarkers Prev,2016,25(1):16-27.
[2]Vaňhara P,Horak P,Pils D.Loss of the oligosaccharyltransferase subunit TUSC3 promotes proliferation and migration ofovarian cancer cells[J].Int J Oncol,2013,42(4):1383-1389.
[3]Horak P,Tomasich E,Vaňhara P,et al.TUSC3 loss alters the ER stress response and accelerates prostate cancer growth in vivo[J].Sci Rep,2014,4:3739.
[4]Yu X,Zhang K,Liu F,et al.Tumor suppressor candidate 3 as a novel predictor for lymph node metastasis in lung cancer patients[J].Oncol Lett,2016,12(6):5099-5105.
[5]Lei J J,Peng R J,Kuang B H,et al.NOP14 suppresses breast cancer progression by inhibiting NRIP1/Wnt/β-catenin pathway[J].Oncotarget,2015,6(28):25701-25714.
[6]Du Y,Liu Z,You L,et al.Pancreatic cancer progression relies upon mutant p53-induced oncogenic signaling mediated by NOP14[J].Cancer Res,2017,77(10):2661-2673.
[7]孟府陶,黄强,黄玫,等.胰腺癌中FFAR4蛋白和mRNA的表达及临床意义[J].临床与实验病理学杂志,2018,34(6):601-604.
[8]姚楠,孙景秋,张琼.肝细胞癌中Dkk-3与Cyclin D1的表达及临床意义[J].临床与实验病理学杂志,2017,33(2):158-161.
[9]Kühn H,Hierlmeier T,Merl J,et al.The Noc-domain containing C-terminus of Noc4p mediates both formation of the Noc4pNop14p submodule and its incorporation into the SSU processome[J].PLo S One,2009,4:e8370.
[10]Montanaro L,TreréD.Changes in ribosome biogenesis may induce cancer by down-regulating the cell tumor suppressor potential[J].Biochim Biophys Acta,2012,1825(1):101-110.
[11]Goyal A,Fi2kin E,Gutschner T,et al.A cautionary tale of senseantisense gene pairs:independent regulation despite inverse correlation of expression[J].Nucleic Acids Res,2017,45(21):12496-12508.
[12]Hannes F,Hammond P,Quarrell O,et al.A microdeletion proximal of the critical deletion region is associated with mild Wolf-Hirschhorn syndrome[J].Am J Med Genet A,2012,158A(5):996-1004.
[13]Li Y,Zhang F,Cong Y,Zhao Y.Identification of potential genes and miRNAs associated with sepsis based on microarray analysis[J].Mol Med Rep,2018,17(5):6227-6234.
[14]Suzuki T,Behnam M,Ronasian F,et al.A homozygous NOP14variant is likely to cause recurrent pregnancy loss[J].J Hum Genet,2018,63(4):425-430.
[2]Vaňhara P,Horak P,Pils D.Loss of the oligosaccharyltransferase subunit TUSC3 promotes proliferation and migration ofovarian cancer cells[J].Int J Oncol,2013,42(4):1383-1389.
[3]Horak P,Tomasich E,Vaňhara P,et al.TUSC3 loss alters the ER stress response and accelerates prostate cancer growth in vivo[J].Sci Rep,2014,4:3739.
[4]Yu X,Zhang K,Liu F,et al.Tumor suppressor candidate 3 as a novel predictor for lymph node metastasis in lung cancer patients[J].Oncol Lett,2016,12(6):5099-5105.
[5]Lei J J,Peng R J,Kuang B H,et al.NOP14 suppresses breast cancer progression by inhibiting NRIP1/Wnt/β-catenin pathway[J].Oncotarget,2015,6(28):25701-25714.
[6]Du Y,Liu Z,You L,et al.Pancreatic cancer progression relies upon mutant p53-induced oncogenic signaling mediated by NOP14[J].Cancer Res,2017,77(10):2661-2673.
[7]孟府陶,黄强,黄玫,等.胰腺癌中FFAR4蛋白和mRNA的表达及临床意义[J].临床与实验病理学杂志,2018,34(6):601-604.
[8]姚楠,孙景秋,张琼.肝细胞癌中Dkk-3与Cyclin D1的表达及临床意义[J].临床与实验病理学杂志,2017,33(2):158-161.
[9]Kühn H,Hierlmeier T,Merl J,et al.The Noc-domain containing C-terminus of Noc4p mediates both formation of the Noc4pNop14p submodule and its incorporation into the SSU processome[J].PLo S One,2009,4:e8370.
[10]Montanaro L,TreréD.Changes in ribosome biogenesis may induce cancer by down-regulating the cell tumor suppressor potential[J].Biochim Biophys Acta,2012,1825(1):101-110.
[11]Goyal A,Fi2kin E,Gutschner T,et al.A cautionary tale of senseantisense gene pairs:independent regulation despite inverse correlation of expression[J].Nucleic Acids Res,2017,45(21):12496-12508.
[12]Hannes F,Hammond P,Quarrell O,et al.A microdeletion proximal of the critical deletion region is associated with mild Wolf-Hirschhorn syndrome[J].Am J Med Genet A,2012,158A(5):996-1004.
[13]Li Y,Zhang F,Cong Y,Zhao Y.Identification of potential genes and miRNAs associated with sepsis based on microarray analysis[J].Mol Med Rep,2018,17(5):6227-6234.
[14]Suzuki T,Behnam M,Ronasian F,et al.A homozygous NOP14variant is likely to cause recurrent pregnancy loss[J].J Hum Genet,2018,63(4):425-430.
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