ROC曲线方法确定恒山仿野生黄芪毛蕊异黄酮葡萄糖苷的含量限度
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摘要
目的:探索确立黄芪药材中毛蕊异黄酮葡萄糖苷含量限度的受试者工作特征(ROC)曲线数理统计方法,以区分山西恒山仿野生芪与甘肃移栽芪毛蕊异黄酮葡萄糖苷含量差异。方法:选取45批共225个山西恒山仿野生芪样本与42批共210个甘肃移栽芪样本为研究对象,采用2015年版《中国药典》黄芪项下毛蕊异黄酮葡萄糖苷含量测定方法测定黄芪样本毛蕊异黄酮葡萄糖苷含量。统计分析数据并采用ROC曲线判定山西恒山仿野生芪与甘肃移栽芪毛蕊异黄酮葡萄糖苷临界值。结果:恒山仿野生芪和甘肃移栽芪毛蕊异黄酮葡萄糖苷平均质量分数分别为0. 107%和0. 039%,两者差异有统计学意义(P <0. 01)。ROC曲线判定恒山仿野生芪与甘肃移栽芪毛蕊异黄酮葡萄糖苷质量分数差异的临界值为0. 069%。该曲线的灵敏度为100%,特异度为93. 33%,模型诊断准确率为0. 978,说明预测结果准确。结论:ROC曲线法可确定山西恒山仿野生芪与甘肃移栽芪毛蕊异黄酮葡萄糖苷临界值,提高恒山仿野生芪毛蕊异黄酮葡萄糖苷含量限度,体现市场"优质优价"规律,为建立优质黄芪中药材标准提供理论基础。
Objective: To explore the receiuer operating characteristic( ROC) curve mathematical statistics method to establish the content limit of isoflavones glucoside contents in Astragali Radix,in order to distinguish the difference in isoflavones glucoside content between Hengshan wild-simulated Astragali Radix in Shanxi and cultivated Astragali Radix in Gansu. Method: A total of 225 samples of 45 batches of wild-simulated Astragali Radix in Hengshan and 210 samples of 42 batches of cultivated Astragali Radix in Gansu were selected as research objects,and isoflavones glucoside contents of these samples were determined by using the method in the edition 2015 Chinese Pharmacopoeia. Data was statistically analyzed,and the ROC curve was used to determine the critical value of wild-simulated Astragali Radix in Hengshan and transplanted Astragali Radix in Gansu. Result:The average contents of isoflavones glucoside in imitative wild Astragali Radix in Henshan and cultivated Astragali Radix in Gansu were 0. 107% and 0. 039% respectively,with statistically significant differences( P < 0. 01). The ROC curve had a critical value of 0. 069%,and its sensitivity and specificity was 100% and 93. 33%,and its model diagnostic accuracy was 0. 978,which indicated accurate prediction results. Conclusion: ROC curve can be used to distinguish the critical value of the wild-simulated Astragali Radix in Hengshan and cultivated Astragali Radix in Gansu. The results reflected the regularity of " high quality and good price" in the market. The research provided a theoretical basis for the establishment of standard of high-quality Chinese herbal medicines.
Objective: To explore the receiuer operating characteristic( ROC) curve mathematical statistics method to establish the content limit of isoflavones glucoside contents in Astragali Radix,in order to distinguish the difference in isoflavones glucoside content between Hengshan wild-simulated Astragali Radix in Shanxi and cultivated Astragali Radix in Gansu. Method: A total of 225 samples of 45 batches of wild-simulated Astragali Radix in Hengshan and 210 samples of 42 batches of cultivated Astragali Radix in Gansu were selected as research objects,and isoflavones glucoside contents of these samples were determined by using the method in the edition 2015 Chinese Pharmacopoeia. Data was statistically analyzed,and the ROC curve was used to determine the critical value of wild-simulated Astragali Radix in Hengshan and transplanted Astragali Radix in Gansu. Result:The average contents of isoflavones glucoside in imitative wild Astragali Radix in Henshan and cultivated Astragali Radix in Gansu were 0. 107% and 0. 039% respectively,with statistically significant differences( P < 0. 01). The ROC curve had a critical value of 0. 069%,and its sensitivity and specificity was 100% and 93. 33%,and its model diagnostic accuracy was 0. 978,which indicated accurate prediction results. Conclusion: ROC curve can be used to distinguish the critical value of the wild-simulated Astragali Radix in Hengshan and cultivated Astragali Radix in Gansu. The results reflected the regularity of " high quality and good price" in the market. The research provided a theoretical basis for the establishment of standard of high-quality Chinese herbal medicines.
引文
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[14]高四云,李科,熊一峰,等.恒山仿野生黄芪绝对生长年限鉴别及黄酮和皂苷积累规律研究[J].药学学报,2018,53(1):147-154.
[15]王军.苦参药用部位发育解剖暨黄芪道地药材的年限鉴别及其次生代谢产物积累研究[D].合肥:安徽中医药大学,2013.
[2]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015:302-303.
[3]叶迎,王瑞海,柏冬,等.甘肃不同产地及不同生长年限红芪和黄芪中4种异黄酮类成分的含量对比[J].中国实验方剂学杂志,2018,24(9):52-58.
[4]杜国军,秦雪梅,李震宇,等.蒙古黄芪主产区2种不同种植模式黄芪药材的质量比较[J].中草药,2013,44(23):3386-3393.
[5]石子仪,鲍忠,姜勇,等.不同来源黄芪药材中毛蕊异黄酮葡萄糖苷和芒柄花素的定量分析[J].中国中药杂志,2007,32(9):779-783.
[6]姚雪莲,裴彩云,王宗权.不同产地、不同采收期黄芪药材及饮片中毛蕊异黄酮葡萄糖苷及芒柄花素含量测定[J].药物分析杂志,2012,32(5):797-801.
[7]杜国军.恒山黄芪道地药材质量标准研究[D].太原:山西大学,2013.
[8]丁峰,石庆平,姜晓东,等. Logistic模型和ROC曲线对参麦注射液致不良反应的预测分析[J].中国中药杂志,2015,40(7):1404-1409.
[9]赵晓利,孙利,黄喜英,等. ROC曲线评价糖化血红蛋白在妊娠期糖尿病诊断中价值探讨[J].河北医学,2016,22(1):99-102.
[10]陈卫中,倪宗瓒,潘晓平,等.用ROC曲线确定最佳临界点和可疑值范围[J].现代预防医学,2005,32(7):729-731.
[11]李洁.表观扩散系数判断肺癌不同组织学类型及增殖程度的应用价值[J].临床医学研究与实践,2018,3(13):1-3.
[12]张军能,张焕棕,陈李好,等.异常凝血酶原(PIVKA-Ⅱ)在原发性肝癌临床诊断中的应用[J].齐齐哈尔医学院学报,2018,39(2):192-195.
[13]冯磊,年士艳,赵阳,等.不同分析策略下应用二分类Logistic回归进行疾病风险评估的结果差异性分析[J].中华检验医学杂志,2018,41(3):232-236.
[14]高四云,李科,熊一峰,等.恒山仿野生黄芪绝对生长年限鉴别及黄酮和皂苷积累规律研究[J].药学学报,2018,53(1):147-154.
[15]王军.苦参药用部位发育解剖暨黄芪道地药材的年限鉴别及其次生代谢产物积累研究[D].合肥:安徽中医药大学,2013.