恩诺沙星曝露下企鹅珍珠贝肠道微生物多样性及优势菌变化规律
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摘要
【目的】明确恩诺沙星曝露下企鹅珍珠贝肠道微生物群落结构尤其是优势菌的变化规律,为企鹅珍珠贝养殖过程中恩诺沙星的科学用药提供参考依据。【方法】针对3个恩诺沙星浓度(0、5和10 mg/L)共9个企鹅珍珠贝肠道样本中的细菌构建16S rRNA文库,再采用Illlumina高通量测序技术从门和属水平上比较分析各处理组间肠道微生物群落结构和多样性的差异。【结果】从9个企鹅珍珠贝肠道样本中共检测到10个菌门,其优势菌门分别是变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、梭杆菌门(Fusobacteria)、螺旋体门(Spirochaetae)、蓝藻门(Cyanobacteria)和厚壁菌门(Firmicutes);共检测到79个菌属,优势菌属主要包括弧菌属(Vibrio)、发光杆菌属(Photobacterium)、嗜冷菌属(Psychrilyobacter)、假交替单胞菌属(Pseudoalteromonas)、疏螺旋体属(Borrelia)、交替单胞菌属(Alteromonas)、微拟球藻属(Nannochioropsis-oceanica)、弓形杆菌属(Arcobacter)、Thalassotalea、Amphritea、Tenacibaculum和微小杆菌属(Exiguobacterium)等。不同恩诺沙星浓度处理下,企鹅珍珠贝肠道的优势菌门与菌属种类丰度差异明显。经恩诺沙星处理后,企鹅珍珠贝肠道变形菌门相对丰度显著升高(P<0.05,下同)、拟杆菌门相对丰度显著降低;弧菌属相对丰度显著升高,微拟球藻属、Aquibacter、BD1-7_clade及希瓦氏菌属(Shewanella)相对丰度则显著降低;此外,弓形杆菌属相对丰度经10 mg/L恩诺沙星处理后显著升高,Psychrobium相对丰度经5 mg/L恩诺沙星处理后显著升高。【结论】经恩诺沙星处理后企鹅珍珠贝肠道微生物的多样性与丰度发生明显变化,且肠道微生物中一些具有特殊功能的优势菌群结构比例也发生变化,该变化规律可为企鹅珍珠贝养殖过程中科学使用恩诺沙星提供参考依据。
【Objective】Intestinal microbial diversity and dominant bacteria change analysis of Pteria penguin(R?ding)under enrofloxacin exposure were studied to provide reference for scientific administration of enrofloxacin during P. penguin culture.【Method】The bacterial 16 S rRNA library was constructed using the nine P. penguin intestinal bacteria samples under three enrofloxacin concentrations(0,5 and 10 mg/L). The differences in intestinal microbial structure and diversity were analyzed and compared among the groups at phylum level and genus level.【Result】The analysisshowed that,ten phyla were detected from the nine P. penguin intestinal samples,and the core phyla included Proteobacteria,Bacteroidetes,Fusobacteria,Spirochaetae,Cyanobacteria and Firmicutes. And 79 genera were detected,the dominant genera included Vibrio,Photobacterium,Psychrilyobacter,Pseudoalteromonas,Borrelia,Alteromonas,Nannochioropsis-oceanica,Arcobacter,Thalassotalea,Amphritea,Tenacibaculum and Exiguobacterium. After different enrofloxacin concentrations treatments,the abundance of dominant bacteria phyla and genera of P. penguin intestinal tract differed greatly. The relative abundance of Proteobacteria increased significantly under enrofloxacin treatment(P<0.05,the same below),while that of Bacteroidertes decreased significantly. Relative abundance of Arcobacter was significantly increased,and relative abundance of Nannochloropsis,Aquibacte,BD1-7_clade and Shewanella were decreased significantly. Relative abundance of Arcobacter significantly increased after treated by 10 mg/L enrofloxacin,and relative abundance of Psychrobium significantly increased after treated by 5 mg/L enrofloxacin.【Conclusion】The results indicate that enrofloxacin treatment can greatly change the intestinal microbial diversity and abundance in P. penguin,the proportions of dominant microbial community structure with specific functions also change. The findings in the present study can be served to the scientific administration of enrofloxacin during aquaculture of P. penguin.
【Objective】Intestinal microbial diversity and dominant bacteria change analysis of Pteria penguin(R?ding)under enrofloxacin exposure were studied to provide reference for scientific administration of enrofloxacin during P. penguin culture.【Method】The bacterial 16 S rRNA library was constructed using the nine P. penguin intestinal bacteria samples under three enrofloxacin concentrations(0,5 and 10 mg/L). The differences in intestinal microbial structure and diversity were analyzed and compared among the groups at phylum level and genus level.【Result】The analysisshowed that,ten phyla were detected from the nine P. penguin intestinal samples,and the core phyla included Proteobacteria,Bacteroidetes,Fusobacteria,Spirochaetae,Cyanobacteria and Firmicutes. And 79 genera were detected,the dominant genera included Vibrio,Photobacterium,Psychrilyobacter,Pseudoalteromonas,Borrelia,Alteromonas,Nannochioropsis-oceanica,Arcobacter,Thalassotalea,Amphritea,Tenacibaculum and Exiguobacterium. After different enrofloxacin concentrations treatments,the abundance of dominant bacteria phyla and genera of P. penguin intestinal tract differed greatly. The relative abundance of Proteobacteria increased significantly under enrofloxacin treatment(P<0.05,the same below),while that of Bacteroidertes decreased significantly. Relative abundance of Arcobacter was significantly increased,and relative abundance of Nannochloropsis,Aquibacte,BD1-7_clade and Shewanella were decreased significantly. Relative abundance of Arcobacter significantly increased after treated by 10 mg/L enrofloxacin,and relative abundance of Psychrobium significantly increased after treated by 5 mg/L enrofloxacin.【Conclusion】The results indicate that enrofloxacin treatment can greatly change the intestinal microbial diversity and abundance in P. penguin,the proportions of dominant microbial community structure with specific functions also change. The findings in the present study can be served to the scientific administration of enrofloxacin during aquaculture of P. penguin.
引文
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