外源水杨酸对草地早熟禾抗褐斑病的诱导与抗病基因PR1和NPR1的表达的影响
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摘要
为了探究外源水杨酸(SA)对草地早熟禾(Poa pratensis)抗褐斑病的诱抗效果,本研究将草地早熟禾品种午夜(Midnight)分为3组,用0.05 mmol·L~(–1) SA和立枯丝核菌进行处理,测定草坪草发病率、病情指数,计算诱抗效果,同时检测相关抗病基因PR1、NPR1表达情况。结果表明,外源SA可以显著(P <0.05)降低草地早熟禾褐斑病的发病率和病情指数,对褐斑病的诱抗效果最高达53%。在喷施SA及接种后,抗病基因PR1和NPR1相对表达量均显著(P <0.05)升高。
To explore the effect of external salicylic acid(SA) on the resistance of Kentucky bluegrass(Poa pratensis) to brown patch, Kentucky bluegrass variety ‘Midnight' was divided into three groups and treated with 0.05 mmol·L–1 SA and Rhizoctonia solani. The percent of disease infection and disease index were measured, and the efficacy of resistance induced was calculated. The relative expression of disease resistance genes PR1 and NPR1 was assessed. The results showed that external SA could significantly(P < 0.05) decrease the plant infection percent and disease index of brown patch on Kentucky bluegrass, and the highest efficacy of resistance induced by SA was 45.53%. After spraying SA and inoculation, the relative expression of PR1 and NPR1 increased significantly(P < 0.05).
To explore the effect of external salicylic acid(SA) on the resistance of Kentucky bluegrass(Poa pratensis) to brown patch, Kentucky bluegrass variety ‘Midnight' was divided into three groups and treated with 0.05 mmol·L–1 SA and Rhizoctonia solani. The percent of disease infection and disease index were measured, and the efficacy of resistance induced was calculated. The relative expression of disease resistance genes PR1 and NPR1 was assessed. The results showed that external SA could significantly(P < 0.05) decrease the plant infection percent and disease index of brown patch on Kentucky bluegrass, and the highest efficacy of resistance induced by SA was 45.53%. After spraying SA and inoculation, the relative expression of PR1 and NPR1 increased significantly(P < 0.05).
引文
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[11]房媛媛,马晖玲.2,3-丁二醇与水杨酸诱导匍匐翦股颖对镰刀菌枯萎病的抗性.草原与草坪,2015, 35(3):83-87.FANG Y Y, MA H L. 2,3-Butanediol and salicylic acid induced resistance of creeping bentgrass to fusarium wilt. Grassland and Lawn,2015, 35(3):83-87.
[12]向妙莲,汪杰,阙海勇,蒋军喜,罗友强,刘冰,宋水林.水杨酸诱导车前草抗菌核病研究.江西植保,2009, 32(3):111-113, 116.XIANG M L, WANG J, QUE H Y, JIANG J X, LUO Y Q, LIU B, SONG S L. Study on the effect of salicylic acid on the antibacterial nuclear disease of plantain. Jiangxi Plant Protection, 2009, 32(3):111-113, 116.
[13]钟小刚,薛应钰,梁巧兰,徐秉良,范祥梅.水杨酸和壳聚糖诱导苹果叶片对斑点落叶病抗性的研究.植物保护,2013,39(4):20-24.ZHONG X G, XUE Y Y, LIANG Q L, XU B L, FAN X M. Studies on resistance of salicylic acid and chitosan to leaf spot and defoliate diseaser in apple leaves. Plant Protection, 2013, 39(4):20-24.
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[15] GUO Y H, YU Y P, WANG D. GhZFP1, a novel CCCH-type zinc finger protein from cotton, enhances salt stress tolerance and fungal disease resistance in transgenic tobacco by interacting witn GZIRD21A and GZIPR5. New Phytologist, 2009, 183(4):62-75.
[16]殷萍萍.日本结缕草响应立枯丝核菌侵染及其转录组学研究.北京:北京林业大学硕士学位论文,2015.YIN P P. Study on the transcriptome of zoysiagrass in response to the infection of Rhizoctonia solani. Master Thesis. Beijing:Beijing Forestry University,2015.
[17] NAKANO Y, ASADAK. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiology, 1981,22(2):867-880.
[18]周丽霞,易克贤,马振宇.苜蓿霜霉病的接种鉴定.甘肃畜牧兽医,1998, 140(3):44.ZHOU L X, YI K X, MA Z Y. Identification of alfafa downy mildew inoculation. Gansu Animalland Veterinary Science, 1998,140(3):44.
[19] ALLEN R D. Dissection of oxidative stress tolerance using transgenic plants. Plant Physiology, 1995, 107(4):1049-1054.
[20]张岗,李依民,张毅.条锈菌诱导的小麦病程相关蛋白TaPR10基因的克隆及特征分析.中国农业科学,2009, 42(1):110-116.ZHANG G, LI Y M, ZHANG Y. Cloning and characterization of a pathogenesis related protein gene TaPR10 from wheat induced by stripe rust pathogen. Scientia Agricultura Sinica, 2009, 42(1):110-116.
[21] SZALONTAI B, JAKAB G. Differential expression of PRLIPs, a pathogenesis-related gene family encoding class 3 lipase-like proteins in Arabidopsis. Acta Biologica Hungarica, 2010, 61(1):156-157.
[22]王坤,王海燕,刘大群.叶锈菌与'TcLr19'小麦互作体系中PR1基因的克隆及分析.河北农业大学学报,2012, 35(2):1-6.WANG K, WANG H Y, LIU D Q. Cloning and analysis of a PR1 gene from'TcLr19'wheat in the defense responses to Puccinia triticina. Journal of Agricultural University of Hebei, 2012, 35(2):1-6.
[23] CAO H, BOWLING S A, GORDON A S. Characterizaion of an Arabidopsis mutant that is nonresponsive to inducers of systemic acquired resistance. Plant Cell, 1994, 6(8):1583-1592.
[2]孙建华,王彦荣,柴琦.28个草地早熟禾品种坪用性状评价.草业科学,2003, 20(12):18-21.SUN J H, WANG Y R, CHAI Q. Turf use quality characteristic assessment for 28 varieties of Poa pratensis. Pratacultual Science,2003,20(12):18-21.
[3]高琪昕,胡新喜,王欢妍.水杨酸诱导植物抗病性机制的研究进展.中国马铃薯,2014, 28(4):238-242.GAO Q X, HU X X, WANG H Y. Research progress in mechanism of plant disease resistance induced by salicylic acid. Chinese Potato Journal,2014, 28(4):238-242.
[4]孙涛,曹致中,马晖玲.水杨酸诱导苜蓿对霜霉病抗性的研究.甘肃农业大学学报,2006, 41(3):61-64.SUN T, CAO Z Z, MA H L. Study on resistence of alfafa to downy mildew induced by SA. Journal of Gansu Agricultural University, 2006, 41(3):61-64.
[5]蔡新忠,郑重,宋凤鸣.水杨酸对水稻幼苗抗瘟性的诱导作用.植物病理学报,1996, 26(1):7-12.CAI X Z, ZHENG Z, SONG F M. Effect of salicylic acid on the induction of resistance to rice seedling blast. Acta Phyto Pathologica Sinica, 1996, 26(1):7-12.
[6]刘凤权,王金生.水杨酸诱导水稻幼苗抗白叶枯病研究.植物保护学报,2000, 27(1):47-52.LIU F Q, WANG J S. Study on the effect of salicylic acid on the resistance of rice seedlings to leaf blight. Journal of Plant Protection,2000, 27(1):47-52.
[7]古燕翔,王代军.外源诱导物水杨酸对草坪型高羊茅弯孢霉叶病抗性影响的研究.中国草地,2003, 25(4):57-61,72.GU Y X, WANG D J. Effect of external salicylic acid on tall fescue on Festuca arundinacea Schreb. Glassland of China, 2003,25(4):57-61,72.
[8]孙艳,杨淑英.水杨酸诱发黄瓜幼苗对霜霉病抗性的研究.植物病理学报,2004, 34(2):187-189.SUN Y, YANG S Y. Effects of SA on resistence of cucumber seedlings to downy mildew. Acta Phyto Pathologica Sinica, 2004,34(2):187-189.
[9]张莹.水杨酸诱导玉米抗大斑病的机理研究.保定:河北农业大学硕士学位论文,2007.ZHANG Y. Study on the mechanism of salicylic acid in maize's resistance to macular disease. Mater Thesis. Baoding:Hebei Agricultural University, 2007.
[10]金一锋.外源性水杨酸诱导月季对黑斑病抗性的研究.哈尔滨:东北农业大学硕士学位论文,2013.JIN Y F. Study on resistance of Chinese rose to black spot disease induced of exogenous salicylic acid. Mater Thesis. Harbin:Northeast Agricultural University, 2013.
[11]房媛媛,马晖玲.2,3-丁二醇与水杨酸诱导匍匐翦股颖对镰刀菌枯萎病的抗性.草原与草坪,2015, 35(3):83-87.FANG Y Y, MA H L. 2,3-Butanediol and salicylic acid induced resistance of creeping bentgrass to fusarium wilt. Grassland and Lawn,2015, 35(3):83-87.
[12]向妙莲,汪杰,阙海勇,蒋军喜,罗友强,刘冰,宋水林.水杨酸诱导车前草抗菌核病研究.江西植保,2009, 32(3):111-113, 116.XIANG M L, WANG J, QUE H Y, JIANG J X, LUO Y Q, LIU B, SONG S L. Study on the effect of salicylic acid on the antibacterial nuclear disease of plantain. Jiangxi Plant Protection, 2009, 32(3):111-113, 116.
[13]钟小刚,薛应钰,梁巧兰,徐秉良,范祥梅.水杨酸和壳聚糖诱导苹果叶片对斑点落叶病抗性的研究.植物保护,2013,39(4):20-24.ZHONG X G, XUE Y Y, LIANG Q L, XU B L, FAN X M. Studies on resistance of salicylic acid and chitosan to leaf spot and defoliate diseaser in apple leaves. Plant Protection, 2013, 39(4):20-24.
[14] BURPEE L, MARTIN B. Biology of rhizoctonia species associated with turgrasses. Plant Disease, 1992, 76(2):112-117.
[15] GUO Y H, YU Y P, WANG D. GhZFP1, a novel CCCH-type zinc finger protein from cotton, enhances salt stress tolerance and fungal disease resistance in transgenic tobacco by interacting witn GZIRD21A and GZIPR5. New Phytologist, 2009, 183(4):62-75.
[16]殷萍萍.日本结缕草响应立枯丝核菌侵染及其转录组学研究.北京:北京林业大学硕士学位论文,2015.YIN P P. Study on the transcriptome of zoysiagrass in response to the infection of Rhizoctonia solani. Master Thesis. Beijing:Beijing Forestry University,2015.
[17] NAKANO Y, ASADAK. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiology, 1981,22(2):867-880.
[18]周丽霞,易克贤,马振宇.苜蓿霜霉病的接种鉴定.甘肃畜牧兽医,1998, 140(3):44.ZHOU L X, YI K X, MA Z Y. Identification of alfafa downy mildew inoculation. Gansu Animalland Veterinary Science, 1998,140(3):44.
[19] ALLEN R D. Dissection of oxidative stress tolerance using transgenic plants. Plant Physiology, 1995, 107(4):1049-1054.
[20]张岗,李依民,张毅.条锈菌诱导的小麦病程相关蛋白TaPR10基因的克隆及特征分析.中国农业科学,2009, 42(1):110-116.ZHANG G, LI Y M, ZHANG Y. Cloning and characterization of a pathogenesis related protein gene TaPR10 from wheat induced by stripe rust pathogen. Scientia Agricultura Sinica, 2009, 42(1):110-116.
[21] SZALONTAI B, JAKAB G. Differential expression of PRLIPs, a pathogenesis-related gene family encoding class 3 lipase-like proteins in Arabidopsis. Acta Biologica Hungarica, 2010, 61(1):156-157.
[22]王坤,王海燕,刘大群.叶锈菌与'TcLr19'小麦互作体系中PR1基因的克隆及分析.河北农业大学学报,2012, 35(2):1-6.WANG K, WANG H Y, LIU D Q. Cloning and analysis of a PR1 gene from'TcLr19'wheat in the defense responses to Puccinia triticina. Journal of Agricultural University of Hebei, 2012, 35(2):1-6.
[23] CAO H, BOWLING S A, GORDON A S. Characterizaion of an Arabidopsis mutant that is nonresponsive to inducers of systemic acquired resistance. Plant Cell, 1994, 6(8):1583-1592.
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