基于表面等离子体共振的核酸传感器研究进展
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摘要
表面等离子体共振(surface plasmon resonance,SPR)生物传感器是一种基于物理光学原理的新型生化分析系统。近年来,因其具有无需标记、高灵敏度、高特异性、实时和快速等优势而广泛应用于各个领域,如临床诊断、转基因成分定性及定量检测、病毒鉴定、环境微生物检测、药物筛选及遗传分析等。它在不需要标记甚至无需纯化生物组分的天然条件下,将核酸探针作为识别因子修饰到传感器芯片表面上,并可实时监测一些特殊的分子互作信息。然而,基于SPR技术的核酸传感器鲜有系统性的报道,因此对有关表面等离子体共振现象的形成及其作为核酸敏感检测手段的原理、应用及发展趋势等问题作了简要的介绍,旨在为生物传感研究提供相应的参考依据。
Surface plasmon resonance( SPR) biosensor is a novel type of biochemical analysis system based on the principle of physical optics. In recent years,due to its advantages of label-free,high sensitivity and specificity,realtime and rapid detection,it has been widely applied in various fields,such as clinical diagnosis,qualitative and quantitative detection of genetically modified organism,virus identification,environmental control,drug screening and genetic analysis. DNA probes without labeling or even purification could be immobilized onto the surface of the sensor chip as a recognition factor,and then the biosensor would monitor some specific binding information. However,there were rare reports about this technology and biosensor for nucleic acids analysis. Therefore,in order to provide some references for biosensor research,this paper briefly introduced the formation of surface plasmon resonance phenomena and its mechanisms,principles,applied fields and development trends as sensitive detection methods for nucleic acids.
Surface plasmon resonance( SPR) biosensor is a novel type of biochemical analysis system based on the principle of physical optics. In recent years,due to its advantages of label-free,high sensitivity and specificity,realtime and rapid detection,it has been widely applied in various fields,such as clinical diagnosis,qualitative and quantitative detection of genetically modified organism,virus identification,environmental control,drug screening and genetic analysis. DNA probes without labeling or even purification could be immobilized onto the surface of the sensor chip as a recognition factor,and then the biosensor would monitor some specific binding information. However,there were rare reports about this technology and biosensor for nucleic acids analysis. Therefore,in order to provide some references for biosensor research,this paper briefly introduced the formation of surface plasmon resonance phenomena and its mechanisms,principles,applied fields and development trends as sensitive detection methods for nucleic acids.
引文
[1]苏二辉,耿国强,赵向阳,等.表面等离子共振生物传感技术在食品安全快速检测中的应用研究进展[J].河南农业大学学报,2007,41(1):113-116.Su E H,Geng G Q,Zhao X X,et al..Study advancement of a surface plasmon resonance biosensor technology for rapid food safety testing[J].J.Henan Agric.Univ.,2007,41(1):113-116.
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[22]Prabowo B A,Alom A,Secario M K,et al..Graphene-based portable SPR sensor for the detection of mycobacterium tuberculosis DNA strain[J].Procedia Engin.,2016,168:541-545.
[23]Mousavi M Z,Chen H Y,Wu S H,et al..Magnetic nanoparticle-enhanced SPR on gold nanoslits for ultrasensitive,label-free detection of nucleic acid biomarkers[J].Analyst,2013,138(9):2740-2748.
[24]汪静.合金及磁性纳米复合粒子在SPR传感器中的应用[D].吉林:吉林大学,硕士学位论文,2012.Wang J.Application of alloy and magnetic nanoparticles in SPRsensor[D].Jilin:Jinlin University,Master Dissertation,2012.
[25]Nastruzzi C,Cortesi R,Esposito E,et al..Liposomes as carriers for DNA-PNA hybrids[J].J.Control Release,2000,68(2):237-49.
[26]Hossain M B,Rana M M.Graphene coated high sensitive surface plasmon resonance biosensor for sensing DNAhybridization[J].J.Sensors,2016,14(2):145-152.
[27]洪夏云.SPR传感器优化及LSPR研究[D].杭州:浙江大学,硕士学位论文,2015.Hong X Y.The optimization on SPR sensor and research on LSPR[D].Hangzhou:Zhejiang University,Master Dissertation,2015.
[28]肖桂娜,蔡继业.基于局域表面等离子体共振效应的光学生物传感器[J].化学进展,2010,22(1):194-200.Xiao G N,Cai J Y.Optical biosensor based on localized surface plasmon resonance effect[J].Prog.Chem.,2010,22(1):194-200.
[29]陈雯菁,元秀华.金属纳米颗粒LSPR光纤生物传感DDA方法研究[J].光学与光电技术,2006,4(4):40-43.Chen W Q,Yuan X H.Investigation of noble metal nanoparticle LSPR optical biosensor based on the DDA method[J].Opt.Optoele Technol.,2006,4(4):40-43.
[30]Malic L,Cui B,Veres T,et al..Enhanced surface plasmon resonance imaging detection of DNA hybridization on periodic gold nanoposts[J].Opt.Lett.,2007,32(21):3092-3094.
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[2]李熠,周金慧,赵静,等.表面等离子共振生物传感技术测定蜂蜜中残留的泰乐菌素[J].中国蜂业,2008,59(1):11-13.Li Y,Zhou J H,Zhao J,et al..Determination of tylosin residue in honey by surface plasmon resonance(SPR)[J].Chin.J.Apic.,2008,59(1):11-13.
[3]路萍萍,孟志云,王敏伟,等.表面等离子共振技术测定药物与人血清白蛋白的相互作用[J].中国药理学与毒理学杂志,2007,21(2):147-151.Lu P P,Meng Z Y,Wang M W,et al..Interactions between drug and human serum albumin investigated using surface plasmon resonance technology[J].Chin.J.Pharmacol.Toxicol.,2007,21(2):147-151.
[4]陈执中.DNA微阵列技术的研究应用进展[J].药物生物技术,2001,8(6):357-360.Chen Z Z.Progress in studies and applications of DNAmicroarray technology[J].Pharm.Biotechnol.,2001,8(6):357-360.
[5]赵丽君,陈楠,江静,等.表面等离子共振技术检测人乳头瘤病毒亚型在宫颈病变中的临床应用[J].中国妇产科临床杂志,2011,12(4):252-255.Zhao L J,Chen N,Jiang J,et al..The clinical application of surface plasmon resonance in detection of human papillomavirus genotypes in patients with cervical lesions[J].Chin.J.Clin.Obstetrics Gynecol.,2011,12(4):252-255.
[6]林钟劝,张波.表面等离子共振生物传感技术及其在临床实验诊断中的应用[J].国际检验医学杂志,2013,34(5):585-587.Lin Z Q,Zhang B.Surface plasma resonance biosensing technology and its application in clinical diagnosis[J].Int.J.Lab.Med.,2013,34(5):585-587.
[7]陈朗东,董中云,吕狄亚,等.表面等离子共振技术在定量分析中的应用和研究[J].药学实践杂志,2018(1):18-23.Chen L D,Dong Z Y,Lv D Y,et al..Application of surface plasmon resonance biosensor in quantitative analysis research[J].J.Pharm.Practice,2018(1):18-23.
[8]Liu Y,Wilson W D.Quantitative analysis of small moleculenucleic acid interactions with a biosensor surface and surface plasmon resonance detection[A].In:Proceedings of the seventh Intearnational Conference on Vacuum Metallurgy[C].Iron and Steel Institute of Japan,2010,1-23.
[9]王海明,钱凯先.表面等离子共振技术在生物分子互作研究中的应用[J].浙江大学学报(工学版),2003,37(3):354-357.Wang H M,Qian K X.Principle of surface plasmon resonance biosensor technology and its application in biomolecular interacting analysis[J].J.Zhenjiang Univ(Engin.Sci.),2003,37(3):354-357.
[10]程慧,黄朝峰,段子渊.SPR生物传感器及其应用进展[J].中国生物工程杂志,2003,23(5):46-49.Cheng H,Huang C F,Duan Z Y.Advances in the application of SPR biosensor[J].J.Chin.Biotechnol.,2003,23(5):46-49.
[11]徐明波.SPR技术在生化分析中的应用[D].上海:华中师范大学,硕士学位论文,2007.Xu M B.Application of SPR technology in biochemical analysis[D].Shanghai:Central China Normal University,Master Dissertation,2007.
[12]赵晓君,陈焕文,宋大千,等.表面等离子体子共振传感器I:基本原理[J].分析仪器,2000(4):1-8.Zhao X J,Chen H W,Song D Q,et al..Surface plasmon resonance sensor,part I:Fundamentals[J].Chin.J.Anal.Instrum.,2000(4):1-8.
[13]吴世康,汪鹏飞.表面等离子共振(SPR)---一种新型化学检测方法的原理[J].影像科学与光化学,2008,26(2):157-168.Wu S K,Wang P F.Surface plasmon resonance(SPR)---The principle of a new method for chemical detection[J].Image.Sci.Photochem.,2008,26(2):157-168.
[14]肖守斌.运用表面等离子共振(SPR)生物传感器检测转基因玉米的研究[J].玉米科学,2009,17(2):38-43.Xiao S B.Detection of transgenic maize using surface plasmon resonance(SPR)DNA biosensor[J].J.Maize Sci.,2009,17(2):38-43.
[15]Zhou J C,Feller B,Hinsberg B,et al..Melting temperature of surface-tethered DNA[J].Colloid Surface A,2015,481:72-79.
[16]滕娟,徐云庆,顾大勇,等.表面等离子体共振传感器技术在检测甲型流感病毒中的应用研究[J].中国国境卫生检疫杂志,2011(3):145-149.Teng J,Xu Q Y,Gu D Y,et al..Surface plasmon resonance biosensor for detecting influenza A virus in human[J].Chin.J.Front.Health Quar.,2011(3):145-149.
[17]Scarano S,Ermini M L,Spiriti M M,et al..Simultaneous detection of transgenic DNA by surface plasmon resonance imaging with potential application to gene doping detection[J].Anal.Chem.,2011,83(16):6245-53.
[18]Sonntag F,Schmieder S,Danz N,et al..Novel lab-on-a-chip system for the label-free detection of DNA hybridization and protein-protein interaction by surface plasmon resonance(SPR)[A].In:Proceedings pf SPIE Europe Microtechnologies for the New Millennium[C].International Society for Optics and Photonics,2009,73650Q-73650Q-9.
[19]梅博,丁利,程云辉,等.纳米材料在SPR生物传感器中的应用进展[J].包装工程,2015(1):24-28.Mei B,Ding L,Chen Y H,et al..Development in application of nanomaterials in SPR biosensors[J].Package Engin.,2015(1):24-28.
[20]段瑞岐,于秀章,兰竹,等.LSPR生物传感器在临床医学检测应用中的研究进展[J].光电工程,2017,44(2):152-160.Duan R Q,Yu X Z,Lan Z,et al..Research progress in applications of LSPR biosensor for clinical medicine detection[J].Opto-Elec.Engin.,2017,44(2):152-160.
[21]宋婷.利用DNA自组装技术检测DNA甲基化及肿瘤细胞[D].济南:山东师范大学,硕士学位论文,2016.Song T.The detection of DNA methylation and tumor cells by DNA self-assembly[J].Jinan:Shandong Normal University,Master Dissertation,2016.
[22]Prabowo B A,Alom A,Secario M K,et al..Graphene-based portable SPR sensor for the detection of mycobacterium tuberculosis DNA strain[J].Procedia Engin.,2016,168:541-545.
[23]Mousavi M Z,Chen H Y,Wu S H,et al..Magnetic nanoparticle-enhanced SPR on gold nanoslits for ultrasensitive,label-free detection of nucleic acid biomarkers[J].Analyst,2013,138(9):2740-2748.
[24]汪静.合金及磁性纳米复合粒子在SPR传感器中的应用[D].吉林:吉林大学,硕士学位论文,2012.Wang J.Application of alloy and magnetic nanoparticles in SPRsensor[D].Jilin:Jinlin University,Master Dissertation,2012.
[25]Nastruzzi C,Cortesi R,Esposito E,et al..Liposomes as carriers for DNA-PNA hybrids[J].J.Control Release,2000,68(2):237-49.
[26]Hossain M B,Rana M M.Graphene coated high sensitive surface plasmon resonance biosensor for sensing DNAhybridization[J].J.Sensors,2016,14(2):145-152.
[27]洪夏云.SPR传感器优化及LSPR研究[D].杭州:浙江大学,硕士学位论文,2015.Hong X Y.The optimization on SPR sensor and research on LSPR[D].Hangzhou:Zhejiang University,Master Dissertation,2015.
[28]肖桂娜,蔡继业.基于局域表面等离子体共振效应的光学生物传感器[J].化学进展,2010,22(1):194-200.Xiao G N,Cai J Y.Optical biosensor based on localized surface plasmon resonance effect[J].Prog.Chem.,2010,22(1):194-200.
[29]陈雯菁,元秀华.金属纳米颗粒LSPR光纤生物传感DDA方法研究[J].光学与光电技术,2006,4(4):40-43.Chen W Q,Yuan X H.Investigation of noble metal nanoparticle LSPR optical biosensor based on the DDA method[J].Opt.Optoele Technol.,2006,4(4):40-43.
[30]Malic L,Cui B,Veres T,et al..Enhanced surface plasmon resonance imaging detection of DNA hybridization on periodic gold nanoposts[J].Opt.Lett.,2007,32(21):3092-3094.
[31]Jang H,Kwak C H,Kim G,et al..Identification of genetically modified DNA found in Roundup Ready soybean using gold nanoparticles[J].Microchim.Acta,2016,183(9):2649-2654..
[32]王琼,唐浩,谢青季,等.电化学表面等离子体共振技术[J].化学进展,2007,19(2):370-376.Wang Q,Tang H,Xie Q J,et al..Electrochemical surface plasmon resonance studies[J].Prog.Chem.,2007,19(2):370-376.
[33]付清丽.电化学表面等离子激元共振技术(EC-SPR)的应用[D].长沙:中南大学,硕士学位论文,2012.Fu L Q.Application of electrochemical surface plasmon resonance[D].Changsha:Zhongnan University,Master Dissertation,2012.
[34]Salamifar S E,Lai R Y.Application of electrochemical surface plasmon resonance spectroscopy for characterization of electrochemical DNA sensors[J].Colloid Surface B,2014,122:835-839.
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