紫苏籽壳原花青素纯化及抗氧化性研究
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摘要
旨在研究紫苏籽壳原花青素的纯化工艺及其体外抗氧化活性。采用大孔吸附树脂法,通过静态、动态实验,确定最佳纯化参数;采用分光光度法检测原花青素清除DPPH、ABTS自由基的能力,评价其抗氧化活性。结果表明:XDA-8是纯化紫苏籽壳原花青素的最优树脂,吸附率为63.41%,解吸率为78.98%。其最佳工艺为:上样流速4 BV/h、上样浓度4 mg/mL、洗脱剂乙醇体积分数70%、洗脱流速4 BV/h。在此条件下,原花青素纯度由5.25%提高到12.10%。紫苏籽壳原花青素纯化物对DPPH、ABTS自由基的半数抑制浓度IC_(50)分别为2.138、0.3699μg/mL,清除能力强于Vc。因此,XDA-8树脂纯化法简单、高效,可用于紫苏籽壳原花青素的纯化,且紫苏籽壳原花青素具有较强的体外抗氧化活性。
The aim is to study the purification technology of procyanidins from perilla seed hull and the antioxidant activity in vitro. The optimal purification parameters of procyanidins were obtained through static and dynamic experiments by using macroporous adsorption resin method. The antioxidant activity of procyanidins against DPPH and ABTS free radicals were evaluated by spectrophotometry. The results showed that XDA-8 was the best resin for purifying procyanidins in perilla seed hull, the adsorption rate was 63.41%,and the desorption rate was 78.98%. The optimal parameters were as follows: sample flow rate of 4 BV/h,sample concentration of 4 mg/mL, ethanol concentration of 70%, and elution flow rate of 4 BV/h. Under these conditions, the purity of procyanidins increased from 5.25% to 12.10%. The half-inhibition concentration(IC_(50)) value of DPPH radical and ABTS radical scavenging activities for purified procyanidins was 2.138 μg/mL and 0.3699 μg/mL, respectively, and the scavenging abilities of purified procyanidins were higher than that of Vc. Therefore, XDA-8 resin purification method is simple and efficient, and can be used for the purification of procyanidins from perilla seed hull, and the purified procyanidins of perilla seed hull have stronger antioxidant activity in vitro.
The aim is to study the purification technology of procyanidins from perilla seed hull and the antioxidant activity in vitro. The optimal purification parameters of procyanidins were obtained through static and dynamic experiments by using macroporous adsorption resin method. The antioxidant activity of procyanidins against DPPH and ABTS free radicals were evaluated by spectrophotometry. The results showed that XDA-8 was the best resin for purifying procyanidins in perilla seed hull, the adsorption rate was 63.41%,and the desorption rate was 78.98%. The optimal parameters were as follows: sample flow rate of 4 BV/h,sample concentration of 4 mg/mL, ethanol concentration of 70%, and elution flow rate of 4 BV/h. Under these conditions, the purity of procyanidins increased from 5.25% to 12.10%. The half-inhibition concentration(IC_(50)) value of DPPH radical and ABTS radical scavenging activities for purified procyanidins was 2.138 μg/mL and 0.3699 μg/mL, respectively, and the scavenging abilities of purified procyanidins were higher than that of Vc. Therefore, XDA-8 resin purification method is simple and efficient, and can be used for the purification of procyanidins from perilla seed hull, and the purified procyanidins of perilla seed hull have stronger antioxidant activity in vitro.
引文
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[24]赵文娟,宋扬,杨洪江.大孔吸附树脂纯化黑果枸杞中的原花青素[J].食品工业科技,2017(22):189-194.
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[27]孙子文.紫苏叶有效成分的提取及生物活性研究[D].太原:中北大学,2014.
[28]白欢欢.花生红衣原花青素的纯化工艺及性质研究[D].无锡:江南大学,2017.
[29]栾连军,雷后亮,周芸,等.莲房原花青素提取纯化工艺研究与抗氧化活性评价[J].中国现代应用药学,2015,32(9):1069-1073.
[30]喻秀娟,郭达,韦琴,等.李子皮原花青素的提取及抗氧化活性研究[J].广州化工,2018,46(4):51-54.
[2]张晓彬,姜文鑫,张琳,等.紫苏的研究进展[J].食品研究与开发,2015,36(7):140-143.
[3]张泽涛,徐娟,李建成.紫苏油的氧化稳定性研究[J].中国油脂,2016,46(10):84-86.
[4]Kim H K,Choi H.Stimulation of acyl-CoA oxidase by alphalinolenic acid-rich perilla oil lowers plasma triacylglycerol level in rats[J].Life Sciences,2005,77(12):1293-1306.
[5]嵇志红,王钦富,王永奇,等.植物提取剂紫苏油对大鼠血压及心率的影响[J].中国临床康复,2004,8(3):464-465.
[6]王亚萍,陈锴,符兆英,等.紫苏子油对衰老小鼠记忆力和对正常小鼠镇静作用的影响[J].中国老年学杂志,2016,36(7):1544-1546.
[7]宋家乐.紫苏籽皮提取物的自由基清除能力及抗癌活性[J].食品工业科技,2012,33(2):180-181,223.
[8]李国清,郑久利,王天元,等.紫苏种子皮抽提物保健作用初探[J].黑龙江医药科学,2003,26(5):44-45.
[9]王天元.紫苏子皮提取物中抗氧化性成分及作用研究[J].林产化学与工业,2002,22(1):63-67.
[10]朱月,李奋梅,王艳丽,等.黑果腺肋花楸原花青素的提取及抑菌性研究[J].食品工业科技,2017,38(2):302-306,341.
[11]段江莲,李琴,李为琴,等.紫苏甲醇提取物的体外抗氧化及抑菌活性研究[J].实验室科学,2014,17(2):8-11.
[12]朱学伸,赵文,林淑鑫,等.黑豆种皮中原花青素的提取和纯化研究[J].现代食品科技,2018,34(1):154-160.
[13]李玉妹,刘春凤,李崎.麦芽中原花青素的提取纯化[J].食品与发酵工业,2014,40(1):244-249.
[14]Zhao G,Gao H,Qiu J,et al.The molecular mechanism of protective effects of grape seed proanthocyanidin extract on reperfusion arrhythmias in rats in vivo[J].Biological and Pharmaceutical Bulletin,2010,33(5):759-767.
[15]官清,张珩,石晓琳.原花青素药用研究进展[J].临床合理用药杂志,2012,05(4):174-175.
[16]杨志娟,曾真,吴晓萍.火龙果皮原花青素提取纯化及定性分析[J].食品科学,2015,36(2):75-79.
[17]庄英斌,刘军海,郭景学.天然活性多酚提取、纯化及功能性研究进展[J].粮食与油脂,2012(8):44-48.
[18]Wang Y Q,Li X U,Cao F L,et al.Negative Pressure CavitationAssisted Extraction and Purification of Proanthocyaidins from Ginkgo biloba Leaves[J].Food Science,2013,34(24):6-10.
[19]Sun Y S,Zhu H F,Wang J H,et al.Isolation and purification of salvianolic acid A and salvianolic acid B from Salvia miltiorrhiza by high-speed counter-current chromatography and comparison of their antioxidant activity[J].Journal of Chromatography B,2009,877(8/9):733-737.
[20]李侠,臧学丽,徐祎博,等.AB-8大孔树脂纯化绿豆皮黄酮工艺优化及纯化前后抗氧化能力比较[J].食品科学,2018,39(10):283-290.
[21]崔丽霞,张志军,李晓君,等.紫苏花色苷纯化工艺研究[J].食品科技,2017(5):204-208.
[22]薛姣.紫苏迷迭香酸提取工艺及其应用研究[D].太原:中北大学,2016.
[23]杨青,何传波,魏好程,等.大孔树脂纯化山竹壳原花青素的研究[J].热带作物学报,2013,34(3):569-573.
[24]赵文娟,宋扬,杨洪江.大孔吸附树脂纯化黑果枸杞中的原花青素[J].食品工业科技,2017(22):189-194.
[25]夏瑶瑶.紫苏籽油及原花青素的提取工艺研究[D].太原:中北大学,2017.
[26]李钰,吴卫,苏华,等.响应面法优化紫苏籽粕超声辅助提取原花青素工艺[J].食品科学,2014,35(4):50-54.
[27]孙子文.紫苏叶有效成分的提取及生物活性研究[D].太原:中北大学,2014.
[28]白欢欢.花生红衣原花青素的纯化工艺及性质研究[D].无锡:江南大学,2017.
[29]栾连军,雷后亮,周芸,等.莲房原花青素提取纯化工艺研究与抗氧化活性评价[J].中国现代应用药学,2015,32(9):1069-1073.
[30]喻秀娟,郭达,韦琴,等.李子皮原花青素的提取及抗氧化活性研究[J].广州化工,2018,46(4):51-54.
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