女金丸HPLC指纹图谱建立及聚类分析和主成分分析
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摘要
目的:建立女金丸的高效液相色谱(HPLC)指纹图谱,并进行聚类分析和主成分分析。方法:采用HPLC法。色谱柱为Agilent ZORBAX Eclipse Plus C_(18),流动相为乙腈-0.2%甲酸溶液(梯度洗脱),流速为1.0 mL/min,检测波长为270 nm,柱温为30℃,进样量为10μL。以黄芩苷为参照,绘制10批样品的HPLC图谱,采用《中药色谱指纹图谱相似度评价软件(2012年版)》进行相似度评价,确定共有峰,并采用SPSS 22.0软件进行聚类分析和主成分分析。结果:10批女金丸样品的HPLC图谱有21个共有峰,相似度均在0.95以上,表明10批样品的化学成分一致性较好,但各成分含量存在差异。欧氏距离为25时,10批样品可聚为2类,S3为一类,其余聚为一类;欧氏距离为5时,后一类又可聚为3类,S2、S4、S6、S10聚为一类,S5、S9聚为一类,S1、S7、S8聚为一类。经主成分分析,3个主成分因子的累积方差贡献率为90.642%,以S3样品的主成分因子综合得分最高、整体质量最好。结论:所建HPLC指纹图谱及聚类分析和主成分分析结果可为女金丸样品的质量控制提供参考。
OBJECTIVE:To establish HPLC fingerprint of Nüjin pills,and to conduct cluster analysis and principal component analysis. METHODS:HPLC method was adopted. The determination was carried out on Agilent ZORBAX Eclipse Plus C_(18) column with mobile phase consisted of acetonitrile-0.2% formic acid solution(gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength was set at 270 nm,and column temperature was 30 ℃. The sample size was 10 μL. Using baicalin as reference,HPLC fingerprints of 10 batches of samples were determined. The similarity of 10 batches of samples was evaluated by TCM Chromatographic Fingerprint Similarity Evaluation System(2012 edition) to confirm common peak. Cluster analysis and principal component analysis were performed by using SPSS 22.0 software. RESULTS:There were 21 common peaks in HPLC chromatograms of 10 batches of samples,the similarity of which was all over 0.95,which indicated that in 10 batches of samples,the chemical composition was consistent,but the contents of these components were different. When euclidean distance was equal to 25,10 batches of sample were divided into two categories;S3 was included in the first category,and others were included in the second category. When euclidean distance was equal to 5,the second category could be divided into three sub-categories,one subcategory was S2,S4,S6 and S10;one sub-category was S5,S9;one sub-category was S1,S7,S8. Through the principal component analysis,the cumulative contribution rate of three main component factors was 90.642%,and comprehensive score of S3 was the highest with the best quality. CONCLUSIONS:Established HPLC fingerprint,the results of cluster analysis and principal component analysis can provide reference for quality evaluation of Nüjin pills.
OBJECTIVE:To establish HPLC fingerprint of Nüjin pills,and to conduct cluster analysis and principal component analysis. METHODS:HPLC method was adopted. The determination was carried out on Agilent ZORBAX Eclipse Plus C_(18) column with mobile phase consisted of acetonitrile-0.2% formic acid solution(gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength was set at 270 nm,and column temperature was 30 ℃. The sample size was 10 μL. Using baicalin as reference,HPLC fingerprints of 10 batches of samples were determined. The similarity of 10 batches of samples was evaluated by TCM Chromatographic Fingerprint Similarity Evaluation System(2012 edition) to confirm common peak. Cluster analysis and principal component analysis were performed by using SPSS 22.0 software. RESULTS:There were 21 common peaks in HPLC chromatograms of 10 batches of samples,the similarity of which was all over 0.95,which indicated that in 10 batches of samples,the chemical composition was consistent,but the contents of these components were different. When euclidean distance was equal to 25,10 batches of sample were divided into two categories;S3 was included in the first category,and others were included in the second category. When euclidean distance was equal to 5,the second category could be divided into three sub-categories,one subcategory was S2,S4,S6 and S10;one sub-category was S5,S9;one sub-category was S1,S7,S8. Through the principal component analysis,the cumulative contribution rate of three main component factors was 90.642%,and comprehensive score of S3 was the highest with the best quality. CONCLUSIONS:Established HPLC fingerprint,the results of cluster analysis and principal component analysis can provide reference for quality evaluation of Nüjin pills.
引文
[1]国家药典委员会.中华人民共和国药典:一部[S].2015年版.北京:中国医药科技出版社,2015:521-522.
[2]靳贝贝,裴香萍,梁慧珍.青皮药材的HPLC指纹图谱建立及聚类分析和主成分分析[J].中国药房,2018,29(24):3336-3339.
[3]高微,陈明生,韦广辉,等.尖尾风药材的HPLC指纹图谱建立及聚类分析和主成分分析[J].中国药房,2018,29(16):2215-2219.
[4]姜红,赵生玉,黄浩洲,等.三勒浆口服液HPLC指纹图谱的建立及多成分定量测定[J].中草药,2017,48(18):3741-3747.
[5]戴德雄,刘根才,朱婷,等.银黄滴丸HPLC指纹图谱研究[J].中草药,2016,47(24):4370-4373.
[6]徐放,李明珠,孙阳,等.齿痛消炎灵颗粒HPLC特征指纹图谱研究及多成分定量测定[J].中草药,2017,48(18):3748-3753.
[7]周杰,商雪莹,佟玲,等.西黄丸HPLC-ELSD指纹图谱研究[J].中草药,2017,48(11):2219-2224.
[8]胡惠君,林龙飞,刘宇灵,等.血栓心脉宁片UPLC-PDA指纹图谱研究[J].中草药,2017,48(20):4268-4274.
[9]明磊,林美妤,张庆贺,等.血栓心脉宁片的HPLC-ELSD指纹图谱研究[J].中国药房,2013,24(7):639-641.
[10]孙冬梅,林晓燕,江洁怡,等.复方芪麻胶囊UPLC指纹图谱建立和同时测定8种成分研究[J].中草药,2016,47(23):4186-4191.
[11]杨莎,周瑞,唐志书,等.秦七风湿方HPLC指纹图谱研究[J].中草药,2017,48(5):924-929.
[12]周卿意骏,张水寒,高尚,等.银黄清肺胶囊HPLC指纹图谱研究[J].中草药,2015,46(9):1314-1320.
[2]靳贝贝,裴香萍,梁慧珍.青皮药材的HPLC指纹图谱建立及聚类分析和主成分分析[J].中国药房,2018,29(24):3336-3339.
[3]高微,陈明生,韦广辉,等.尖尾风药材的HPLC指纹图谱建立及聚类分析和主成分分析[J].中国药房,2018,29(16):2215-2219.
[4]姜红,赵生玉,黄浩洲,等.三勒浆口服液HPLC指纹图谱的建立及多成分定量测定[J].中草药,2017,48(18):3741-3747.
[5]戴德雄,刘根才,朱婷,等.银黄滴丸HPLC指纹图谱研究[J].中草药,2016,47(24):4370-4373.
[6]徐放,李明珠,孙阳,等.齿痛消炎灵颗粒HPLC特征指纹图谱研究及多成分定量测定[J].中草药,2017,48(18):3748-3753.
[7]周杰,商雪莹,佟玲,等.西黄丸HPLC-ELSD指纹图谱研究[J].中草药,2017,48(11):2219-2224.
[8]胡惠君,林龙飞,刘宇灵,等.血栓心脉宁片UPLC-PDA指纹图谱研究[J].中草药,2017,48(20):4268-4274.
[9]明磊,林美妤,张庆贺,等.血栓心脉宁片的HPLC-ELSD指纹图谱研究[J].中国药房,2013,24(7):639-641.
[10]孙冬梅,林晓燕,江洁怡,等.复方芪麻胶囊UPLC指纹图谱建立和同时测定8种成分研究[J].中草药,2016,47(23):4186-4191.
[11]杨莎,周瑞,唐志书,等.秦七风湿方HPLC指纹图谱研究[J].中草药,2017,48(5):924-929.
[12]周卿意骏,张水寒,高尚,等.银黄清肺胶囊HPLC指纹图谱研究[J].中草药,2015,46(9):1314-1320.
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