模拟油藏条件下内源微生物群落空间分布规律
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摘要
【背景】油藏内源微生物群落是开展内源微生物驱油技术的物质基础,由于油藏多孔介质取样技术难度大、成本高,实施内源微生物驱油后从注入端到产出端多孔介质中的内源微生物空间分布规律尚不明确。【目的】通过室内长岩心连续驱替实验模拟油藏内源微生物驱油过程,分析实施后不同空间位点油砂上吸附的内源微生物群落结构,揭示从注入端到产出端内源微生物群落的空间分布规律。【方法】借助高通量测序技术及荧光定量PCR技术解析不同空间位点油砂原位微生物群落信息。【结果】注入端到产出端不同空间位点生态环境的差异及菌属间的相互作用造成油藏内源微生物群落空间分布差异,存在明显的好氧、厌氧空间演替变化规律。岩心前端主要存在一些好氧类的产生物表面活性剂类微生物如假单胞菌属,岩心中部主要存在兼性和厌氧类的微生物如地芽孢杆菌、厌氧杆菌属,岩心末端主要分布严格厌氧类细菌和产甲烷古菌,厌氧类微生物代谢产生的H2、CO2和乙酸分子可以为产甲烷古菌提供代谢底物。【结论】通过室内物模油砂研究,首次明确了内源微生物群落在多孔介质中从注入端到产出端的空间分布规律,证实油藏内源微生物的好氧、厌氧空间接替分布规律,深化了对油藏内源微生物的认识。
[Background] The indigenous microbial community in the porous medium of the reservoir is the basis for the indigenous microorganism enhanced oil recovery. Because of the difficulty and high cost of the sampling technique from porous media in the reservoir, the spatial distribution of indigenous microbes from the injected into the output of porous media after the indigenous microorganism flooding has not been clarified. [Objective] Simulation of indigenous microbial enhanced oil recovery process was done via long core dynamic continuous displacement experiment. The spatial distribution of the indigenous microbial communities from injection to output end was revealed by analysis the internal microbial community structure adsorbed on the oil sand of different spatial loci after implementation.[Methods] The microbial community structure adsorbed on the oil sand of different space sites of core was analyzed by the high-throughput sequencing and fluorescent quantitative PCR. [Results] From injection to output end, the difference in the ecological environment of porous media and microbiological interaction lead to the spatial difference of the microbial community. There has been a clear law of aerobic to anaerobic succession. Aerobic microbes are mainly distributed in the front of the core, such as Pseudomonas, which can drive the crude oil in the front of the core by producing the biological surfactants. Facultative and anaerobic microorganisms such as Geobacillus and Anaerobaculum are mainly in the middle of the core, while anaerobic bacteria and methanogens are mainly at the end of core.Anaerobic bacteria produce H2, CO2 and small molecular acids through the anaerobic metabolism of organic nutrition, which provides a metabolic substrate for the methanogens at the end of the core.[Conclusion] Aerobic and anaerobic succession spatial distribution of indigenous microorganisms in the porous media was clarified for the first time, and the understanding of the indigenous microorganisms in the reservoir was further deepened.
[Background] The indigenous microbial community in the porous medium of the reservoir is the basis for the indigenous microorganism enhanced oil recovery. Because of the difficulty and high cost of the sampling technique from porous media in the reservoir, the spatial distribution of indigenous microbes from the injected into the output of porous media after the indigenous microorganism flooding has not been clarified. [Objective] Simulation of indigenous microbial enhanced oil recovery process was done via long core dynamic continuous displacement experiment. The spatial distribution of the indigenous microbial communities from injection to output end was revealed by analysis the internal microbial community structure adsorbed on the oil sand of different spatial loci after implementation.[Methods] The microbial community structure adsorbed on the oil sand of different space sites of core was analyzed by the high-throughput sequencing and fluorescent quantitative PCR. [Results] From injection to output end, the difference in the ecological environment of porous media and microbiological interaction lead to the spatial difference of the microbial community. There has been a clear law of aerobic to anaerobic succession. Aerobic microbes are mainly distributed in the front of the core, such as Pseudomonas, which can drive the crude oil in the front of the core by producing the biological surfactants. Facultative and anaerobic microorganisms such as Geobacillus and Anaerobaculum are mainly in the middle of the core, while anaerobic bacteria and methanogens are mainly at the end of core.Anaerobic bacteria produce H2, CO2 and small molecular acids through the anaerobic metabolism of organic nutrition, which provides a metabolic substrate for the methanogens at the end of the core.[Conclusion] Aerobic and anaerobic succession spatial distribution of indigenous microorganisms in the porous media was clarified for the first time, and the understanding of the indigenous microorganisms in the reservoir was further deepened.
引文
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[9]Bi YQ,Yu L,Xiu JL,et al.Migration and retention mechanism of microorganisms for oil recovery in porous media[J].Acta Petrolei Sinica,2017,38(1):91-98(in Chinese)毕永强,俞理,修建龙,等.采油微生物在多孔介质中的迁移滞留机制[J].石油学报,2017,38(1):91-98
[10]Feng QX,Dou SJ,Yang HJ,et al.Migration and growth of microorganism in porous medium[J].Acta Scientiarum Naturalium Universitatis Nankaiensis,2003,36(1):126-128(in Chinese)冯庆贤,窦松江,杨怀军,等.采油微生物在多孔介质中的运移与生长实验研究[J].南开大学学报:自然科学版,2003,36(1):126-128
[11]Liu QQ,Zheng JT,Liu HC.Diversity analysis of microbial community in oil reservoir under artificial simulation of porous medium[J].China Biogas,2014,32(2):9-14(in Chinese)刘全全,郑经堂,刘海昌.油藏微生物在人工模拟的多孔介质下的多样性分析[J].中国沼气,2014,32(2):9-14
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[14]Kuisiene N,Raugalas J,Stuknyte M,et al.Identification of the genus Geobacillus using genus-specific primers,based on the16S-23S rRNA gene internal transcribed spacer[J].FEMSMicrobiology Letters,2007,277(2):165-172
[15]Geetha SJ,Banat IM,Joshi SJ.Biosurfactants:production and potential applications in microbial enhanced oil recovery(MEOR)[J].Biocatalysis and Agricultural Biotechnology,2018,14:23-32
[16]Steinberg LM,Regan JM.Phylogenetic comparison of the methanogenic communities from an acidic,oligotrophic fen and an anaerobic digester treating municipal wastewater sludge[J].Applied and Environmental Microbiology,2008,74(21):6663-6671
[17]Li CF,Li Y,Cao YB,et al.Distribution of the lipopeptide biosurfactant-producing microbes in oil reservoir environment[J].Acta Petrolei Sinica,2015,36(9):1122-1126(in Chinese)李彩风,李阳,曹嫣镔,等.油藏环境产脂肽类表面活性剂微生物的分布[J].石油学报,2015,36(9):1122-1126
[18]Rees GN,Patel BK,Grassia GS,et al.Anaerobaculum thermoterrenum gen.nov.,sp.nov.,a novel,thermophilic bacterium which ferments citrate[J].International Journal of Systematic and Evolutionary Microbiology,1997,47(1):150-154
[19]Niu LL,Song L,Liu XL,et al.Tepidimicrobium xylanilyticum sp.nov.,an anaerobic xylanolytic bacterium,and emended description of the genus Tepidimicrobium[J].International Journal of Systematic and Evolutionary Microbiology,2009,59(11):2698-2701
[20]Cheng L,Dai LR,Li X,et al.Isolation and characterization of Methanothermobacter crinale sp.nov.,a novel hydrogenotrophic methanogen from the Shengli Oil Field[J].Applied and Environmental Microbiology,2011,77(15):5212-5219
[21]Song YT.Regulation of microbial growth and metabolism and oil displacement under different gas distribution conditions[J].Chinese Journal of Applied and Environmental Biology,2017,23(6):974-978(in Chinese)宋永亭.不同配气条件下微生物驱油生长代谢规律[J].应用与环境生物学报,2017,23(6):974-978
[22]Fang XY,Li JB,Rui JP,et al.Research progress in biochemical pathways of methanogenesis[J].Chinese Journal of Applied and Environmental Biology,2015,21(1):1-9(in Chinese)方晓瑜,李家宝,芮俊鹏,等.产甲烷生化代谢途径研究进展[J].应用与环境生物学报,2015,21(1):1-9
[23]He YL,She YH,Zhang F,et al.Research on the degradation of the crude oil after the polymer flooding in the north of the Xing4~6 block[J].Science Technology and Engineering,2013,13(23):6697-6704(in Chinese)何延龙,佘跃惠,张凡,等.杏四~六面积北部聚驱后微生物对原油的降解作用研究[J].科学技术与工程,2013,13(23):6697-6704
[24]Ueda K,Beppu T.Lessons from studies of Symbiobacterium thermophilum,a unique syntrophic bacterium[J].Bioscience,Biotechnology,and Biochemistry,2007,71(5):1115-1121
[25]Gao PK,Ma T,Liu RL.Microbial metabolic characteristics and ecological controlling in petroleum reservoir-a review[J].Acta Microbiologica Sinica,2011,51(6):711-717(in Chinese)高配科,马挺,刘如林.油藏微生物的代谢特征和生态结构调控[J].微生物学报,2011,51(6):711-717
[26]Jones DM,Head IM,Gray ND,et al.Crude-oil biodegradation via methanogenesis in subsurface petroleum reservoirs[J].Nature,2008,451(7175):176-180
[27]Yang ZY,Shi M,Wang DW,et al.Study on authigenous microorganism community distribution and oil recovery mechanism in Daqing Oilfield[J].Acta Petrolei Sinica,2006,27(S1):95-100,105(in Chinese)杨振宇,石梅,王大威,等.大庆油田本源微生物群落分布及采油机理研究[J].石油学报,2006,27(S1):95-100,105
[28]Zhao LX,Gao PK,Cao MN,et al.Research on population structure and distribution characteristic of indigenous microorganism in post-polymer-flooding oil reservoir[J].Environmental Science,2012,33(2):625-632(in Chinese)赵玲侠,高配科,曹美娜,等.大庆聚驱后油藏内源微生物群落结构解析与分布特征研究[J].环境科学,2012,33(2):625-632
[29]Kaster KM,Hiorth A,Kjeilen-Eilertsen G,et al.Mechanisms involved in microbially enhanced oil recovery[J].Transport in Porous Media,2012,91(1):59-79
[2]Xiang TS,Wang L,Zhang M.Microorganism of petroleum reservoir and application in oil industry[J].Biotechnology,2005,15(4):87-90(in Chinese)向廷生,王莉,张敏.油藏微生物及其在石油工业中的应用[J].生物技术,2005,15(4):87-90
[3]Wang L,Nie Y,Song XM,et al.Microbial community in petroleum reservoir and its pressure adaptation mechanisms[J].Microbiology China,2016,43(11):2495-2505(in Chinese)王璐,聂勇,宋新民,等.油藏微生物及其压力适应机制[J].微生物学通报,2016,43(11):2495-2505
[4]Zhou F.Microbial diversity of production fluids from oil reservoirs and their response to anaerobic cultivations[D].Shanghai:Master’s Thesis of East China University of Science and Technology,2012(in Chinese)周昉.油藏产出液菌群多样性及其对厌氧培养响应的分析[D].上海:华东理工大学硕士学位论文,2012
[5]Wang H,Song ZY,Hao B,et al.Relationship between structures of microbial colony in produced fluids and oil production performance in microbial flooding oil field[J].Acta Petrolei Sinica,2013,34(3):535-539(in Chinese)王慧,宋智勇,郝滨,等.微生物驱产出液群落结构与现场生产动态的关系[J].石油学报,2013,34(3):535-539
[6]Song ZY,Zhao FM,Sun GZ,et al.Long-term dynamics of microbial communities in a high-permeable petroleum reservoir reveals the spatiotemporal relationship between community and oil recovery[J].Energy&Fuels,2017,31(10):10588-10597
[7]Li H,Mu BZ.Recent advances in molecular microbial ecology of petroleum reservoirs[J].Microbiology China,2008,35(5):803-808(in Chinese)李辉,牟伯中.油藏微生物多样性的分子生态学研究进展[J].微生物学通报,2008,35(5):803-808
[8]Ren HY,Song ZY,Li FJ,et al.Comparison of bacterial community structures in Shengli oil reservoir of different time point[J].Microbiology China,2011,38(4):561-568(in Chinese)任红燕,宋志勇,李霏霁,等.胜利油藏不同时间细菌群落结构的比较[J].微生物学通报,2011,38(4):561-568
[9]Bi YQ,Yu L,Xiu JL,et al.Migration and retention mechanism of microorganisms for oil recovery in porous media[J].Acta Petrolei Sinica,2017,38(1):91-98(in Chinese)毕永强,俞理,修建龙,等.采油微生物在多孔介质中的迁移滞留机制[J].石油学报,2017,38(1):91-98
[10]Feng QX,Dou SJ,Yang HJ,et al.Migration and growth of microorganism in porous medium[J].Acta Scientiarum Naturalium Universitatis Nankaiensis,2003,36(1):126-128(in Chinese)冯庆贤,窦松江,杨怀军,等.采油微生物在多孔介质中的运移与生长实验研究[J].南开大学学报:自然科学版,2003,36(1):126-128
[11]Liu QQ,Zheng JT,Liu HC.Diversity analysis of microbial community in oil reservoir under artificial simulation of porous medium[J].China Biogas,2014,32(2):9-14(in Chinese)刘全全,郑经堂,刘海昌.油藏微生物在人工模拟的多孔介质下的多样性分析[J].中国沼气,2014,32(2):9-14
[12]Ren HY,Zhang XJ,Song ZY,et al.Comparison of microbial community compositions of injection and production well samples in a long-term water-flooded petroleum reservoir[J].PLoS One,2011,6(8):e23258
[13]Gao PK,Ma T,Zhao LX,et al.Screening,optimization and evaluation of indigenous microorganism activation system in Zhan 3 block,Shengli oilfield[J].CIESC Journal,2011,62(7):2005-2012(in Chinese)高配科,马挺,赵玲侠,等.胜利油田沾3区块内源微生物激活剂的筛选、优化及效果评价[J].化工学报,2011,62(7):2005-2012
[14]Kuisiene N,Raugalas J,Stuknyte M,et al.Identification of the genus Geobacillus using genus-specific primers,based on the16S-23S rRNA gene internal transcribed spacer[J].FEMSMicrobiology Letters,2007,277(2):165-172
[15]Geetha SJ,Banat IM,Joshi SJ.Biosurfactants:production and potential applications in microbial enhanced oil recovery(MEOR)[J].Biocatalysis and Agricultural Biotechnology,2018,14:23-32
[16]Steinberg LM,Regan JM.Phylogenetic comparison of the methanogenic communities from an acidic,oligotrophic fen and an anaerobic digester treating municipal wastewater sludge[J].Applied and Environmental Microbiology,2008,74(21):6663-6671
[17]Li CF,Li Y,Cao YB,et al.Distribution of the lipopeptide biosurfactant-producing microbes in oil reservoir environment[J].Acta Petrolei Sinica,2015,36(9):1122-1126(in Chinese)李彩风,李阳,曹嫣镔,等.油藏环境产脂肽类表面活性剂微生物的分布[J].石油学报,2015,36(9):1122-1126
[18]Rees GN,Patel BK,Grassia GS,et al.Anaerobaculum thermoterrenum gen.nov.,sp.nov.,a novel,thermophilic bacterium which ferments citrate[J].International Journal of Systematic and Evolutionary Microbiology,1997,47(1):150-154
[19]Niu LL,Song L,Liu XL,et al.Tepidimicrobium xylanilyticum sp.nov.,an anaerobic xylanolytic bacterium,and emended description of the genus Tepidimicrobium[J].International Journal of Systematic and Evolutionary Microbiology,2009,59(11):2698-2701
[20]Cheng L,Dai LR,Li X,et al.Isolation and characterization of Methanothermobacter crinale sp.nov.,a novel hydrogenotrophic methanogen from the Shengli Oil Field[J].Applied and Environmental Microbiology,2011,77(15):5212-5219
[21]Song YT.Regulation of microbial growth and metabolism and oil displacement under different gas distribution conditions[J].Chinese Journal of Applied and Environmental Biology,2017,23(6):974-978(in Chinese)宋永亭.不同配气条件下微生物驱油生长代谢规律[J].应用与环境生物学报,2017,23(6):974-978
[22]Fang XY,Li JB,Rui JP,et al.Research progress in biochemical pathways of methanogenesis[J].Chinese Journal of Applied and Environmental Biology,2015,21(1):1-9(in Chinese)方晓瑜,李家宝,芮俊鹏,等.产甲烷生化代谢途径研究进展[J].应用与环境生物学报,2015,21(1):1-9
[23]He YL,She YH,Zhang F,et al.Research on the degradation of the crude oil after the polymer flooding in the north of the Xing4~6 block[J].Science Technology and Engineering,2013,13(23):6697-6704(in Chinese)何延龙,佘跃惠,张凡,等.杏四~六面积北部聚驱后微生物对原油的降解作用研究[J].科学技术与工程,2013,13(23):6697-6704
[24]Ueda K,Beppu T.Lessons from studies of Symbiobacterium thermophilum,a unique syntrophic bacterium[J].Bioscience,Biotechnology,and Biochemistry,2007,71(5):1115-1121
[25]Gao PK,Ma T,Liu RL.Microbial metabolic characteristics and ecological controlling in petroleum reservoir-a review[J].Acta Microbiologica Sinica,2011,51(6):711-717(in Chinese)高配科,马挺,刘如林.油藏微生物的代谢特征和生态结构调控[J].微生物学报,2011,51(6):711-717
[26]Jones DM,Head IM,Gray ND,et al.Crude-oil biodegradation via methanogenesis in subsurface petroleum reservoirs[J].Nature,2008,451(7175):176-180
[27]Yang ZY,Shi M,Wang DW,et al.Study on authigenous microorganism community distribution and oil recovery mechanism in Daqing Oilfield[J].Acta Petrolei Sinica,2006,27(S1):95-100,105(in Chinese)杨振宇,石梅,王大威,等.大庆油田本源微生物群落分布及采油机理研究[J].石油学报,2006,27(S1):95-100,105
[28]Zhao LX,Gao PK,Cao MN,et al.Research on population structure and distribution characteristic of indigenous microorganism in post-polymer-flooding oil reservoir[J].Environmental Science,2012,33(2):625-632(in Chinese)赵玲侠,高配科,曹美娜,等.大庆聚驱后油藏内源微生物群落结构解析与分布特征研究[J].环境科学,2012,33(2):625-632
[29]Kaster KM,Hiorth A,Kjeilen-Eilertsen G,et al.Mechanisms involved in microbially enhanced oil recovery[J].Transport in Porous Media,2012,91(1):59-79
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