生物海绵铁体系好氧反硝化菌株筛选及其脱氮影响因素分析
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摘要
采用氨氮模拟废水对生物海绵铁体系进行驯化,筛选出一株具有好氧反硝化功能的铁细菌,并对菌株生理生化及反硝化特性进行研究。经BTB培养基、Winogradsky铁细菌固体培养基初筛,结合反硝化性能测定,对菌株进行分离筛选。经形态观察、生理生化鉴定和16S r DNA序列分析,对菌株进行鉴定。研究碳源、海绵铁加量、pH值、温度及C/N对其生长量、反硝化性能的影响。筛选得到一株具有好氧反硝化功能的铁细菌H5,经鉴定为反硝化无色杆菌属(Achromobacter denitrificans sp.)。反硝化特性试验结果表明:该菌最佳碳源、海绵铁投加量、温度、pH值和C/N分别为酒石酸钾钠、1 mg/L、30℃、7、17∶1。该菌在生物脱氮方面具有明显优势。
The ammonia sponge nitrogen was used to simulate the biological sponge iron system,and an aerobic denitrification iron bacteria was screened. The physiological and biochemical and denitrification characteristics of the strain were studied. The strains were screened by BTB medium and Winogradsky iron bacteria solid medium,and the denitrification performance was tested. The strain was identified by morphological observation,physiological and biochemical identification and 16 S r DNA sequence analysis. The effects of carbon source,sponge iron dosage,pH value,temperature and C/N on the growth and denitrification performance were studied. A strain of iron bacteria H5 with aerobic denitrification function was screened and identified as Achromobacter denitrificans sp. The denitrification characteristics showed that the optimum carbon source,sponge iron dosage,temperature,pH value and C/N were potassium sodium tartrate,1 mg/L,30 ℃,7,17 ∶ 1,respectively. The results showed that the iron bacteria H5 had obvious advantages in biological nitrogen removal.
The ammonia sponge nitrogen was used to simulate the biological sponge iron system,and an aerobic denitrification iron bacteria was screened. The physiological and biochemical and denitrification characteristics of the strain were studied. The strains were screened by BTB medium and Winogradsky iron bacteria solid medium,and the denitrification performance was tested. The strain was identified by morphological observation,physiological and biochemical identification and 16 S r DNA sequence analysis. The effects of carbon source,sponge iron dosage,pH value,temperature and C/N on the growth and denitrification performance were studied. A strain of iron bacteria H5 with aerobic denitrification function was screened and identified as Achromobacter denitrificans sp. The denitrification characteristics showed that the optimum carbon source,sponge iron dosage,temperature,pH value and C/N were potassium sodium tartrate,1 mg/L,30 ℃,7,17 ∶ 1,respectively. The results showed that the iron bacteria H5 had obvious advantages in biological nitrogen removal.
引文
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[24]王兆阳,陈国耀,姜珂.等. 1株耐冷兼性嗜碱好氧反硝化菌的分离鉴定及反硝化特性[J].环境科学,2014,35(6):2341-2348.
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[30]田雪雪,程玉立,张圆圆,等.一株异养硝化-好氧反硝化功能菌的分离鉴定及其脱氮特性[J].环境工程学报,2017,11(2):1269-1275.
[31]王新奇,程爱华.生物海绵铁去除生活污水中氨氮的性能研究[J].科学技术与工程,2014,14(9):1671-1815.
[32] Jiang H X,Wang,Xue S H,et al. Isolation,Identification and characterization of a methyl parathion degrading bacterium[J].Journal of Agro Environment Science,2005,24(5):962-965.
[2] Qu J H,Fan M. The current state of water quality and technology development for water pollution control in China[J]. Environmental Science&Technology,2010,4(6):519-560.
[3]张蔚萍,陈建中.低碳高浓度含氮废水的生物脱氮技术[J].环境保护,2003,31(6):20-21.
[4] Pynaert K,Wyffels S,Srengers R,et al. Oxygen-limited nitrogen removal in a lab-scale rotating biological contactor treating an ammonium-rich wastewater[J]. Water Science&Technology,2002,45(10):357-363.
[5]周少奇,周吉林.生物脱氮新技术研究进展[J].环境污染治理技术与设备,2000,1(6):11-20.
[6]梁书诚,赵敏,卢磊,等.好氧反硝化菌脱氮特性研究进展[J].应用生态学报,2010,21(6):1581-1588.
[7]郭焱,张召基,陈少华.好氧反硝化微生物学机理与应用研究进展[J].微生物学通报,2016,43(11):2480-2487.
[8]陈玲,白洁,赵阳国,等.分离于河口区芦苇湿地1株好氧反硝化菌的鉴定及其反硝化特性[J].微生物学报,2016,56(8):1314-1325.
[9]韩永和,章文贤,庄志刚,等.耐盐好氧反硝化菌A-13菌株的分离鉴定及其反硝化特性[J].微生物学报,2013,53(1):47-58.
[10] Wan C L,Yang X,Lee D J,et al. Aerobic denitrification by novel isolated strain using NO2--N as nitrogen source[J]. Bioresource Technology,2011,102(15):7244-7248.
[11] Miyahara M,Kim S W,Zhou S,et al. Survival of the aerobic denitrifier Pseudomonas stutzeri strain TR2 during co-culture with activated sludge under denitrifying conditions[J]. Bioscience,Biotechnology,and Biochemistry,2012,76(3).
[12]田雪雪,程玉立,张圆圆,等.一株异养硝化-好氧反硝化功能菌的分离鉴定及其脱氮特性[J].环境工程学报,2017,11(2):1269-1275.
[13] Yao S,Ni J R,Ma T,et al. Heterotrophic nitrification and aerobic denitrification at low temperature by a newly isolated bacterium,Acinetobacter sp. HA2[J]. Bioresource Technology,2013,139:80-86.
[14] Liu Y X,Wang Y,Li Y,et al. Nitrogen removal characteristics of heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis C16[J]. Chinese Journal of Chemical Engineering,2015,23(5):827-834.
[15] Shi Z,Zhang Y,Zhou J T,et al. Biological removal of nitrate and ammonium under aerobic atmosphere by Paracoccus versutus LYM[J]. Bioresource Technology,2013,148:144-148.
[16]李杰,王亚娥.生物海绵铁的制备及其在水处理中的应用:ZL200610104990. 9[P]. 2009-4-29.
[17]李正,王亚娥,李杰.污泥浓度对生物海绵铁体系中Fe0的腐蚀影响研究[J].环境科学与技术,2014,37(3):7-11.
[18]李杰,王亚娥,王志盈,等.生物海绵铁在生活污水脱氮除磷中的应用研究[J].中国给水排水,2007,23(1):97-100.
[19]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001.
[20]布坎南·R E,吉本斯·N E.伯杰氏细菌鉴定手册[M].北京:科学出版社,1984.
[21]国家环境保护总局水和废水监测分析方法编委会.水和废水监测分析方法[M]. 4版.北京:中国环境科学出版社,2002.
[22]许涛,王国英,岳秀萍. Diaphorobacter sp.PDB3菌好氧反硝化脱氮特性[J].中国环境科学,2018,38(6):2321-2328.
[23]郝敏娜,杨云龙.高温好氧反硝化菌的分离鉴定及脱氮特性[J].环境工程学报,2014,8(7):3058-3062.
[24]王兆阳,陈国耀,姜珂.等. 1株耐冷兼性嗜碱好氧反硝化菌的分离鉴定及反硝化特性[J].环境科学,2014,35(6):2341-2348.
[25]孙庆花,于德爽,张培玉,等. 1株海洋异养硝化-好氧反硝化菌的分离鉴定及其脱氮特性[J].环境科学,2016,37(2):647-654.
[26]姜磊.好氧反硝化菌的筛选及其反硝化特性研究[D].沈阳:辽宁大学,2012.
[27] Gupta A B. Thiosphaera pantotropha:a sulphur bacterium capable of simultaneous heterotrophic nitrification and aerobic denitrification[J]. Enzyme and Microbial Technology,1997,21(8):589-595.
[28]张苗,黄少斌.高温好氧反硝化菌的分离鉴定及其反硝化性能研究[J].环境科学,2011,32(1):259-265.
[29]司圆圆,陈兴汉,许瑞雯,等.好氧反硝化细菌脱氮研究进展[J].山东化工,2018,47(4):157-158.
[30]田雪雪,程玉立,张圆圆,等.一株异养硝化-好氧反硝化功能菌的分离鉴定及其脱氮特性[J].环境工程学报,2017,11(2):1269-1275.
[31]王新奇,程爱华.生物海绵铁去除生活污水中氨氮的性能研究[J].科学技术与工程,2014,14(9):1671-1815.
[32] Jiang H X,Wang,Xue S H,et al. Isolation,Identification and characterization of a methyl parathion degrading bacterium[J].Journal of Agro Environment Science,2005,24(5):962-965.
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