基于活性和Plackett-Burnman联合Box-Behnken设计的复方栀子金花提取工艺优化
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摘要
目的:优化复方栀子金花的提取工艺条件。方法:以复方栀子金花提取的干膏率、栀子苷转移率、黄芩苷转移率及其抗肿瘤活性和抗炎活性为评价指标,利用Plackett-Burnman联合Box-Behnken设计对复方栀子金花的提取工艺进行考察,确定最佳提取工艺参数。结果:最佳提取条件为44倍量29%的乙醇,回流提取3次,每次3h。结论:本研究确定的提取工艺合理可行,能够缩短实验周期,显著提高有效成分的转移率,且结合活性评价,筛选结果更具有指导意义。
Objective: To optimize the extraction process conditions of Compound Zhizi Jinhua. Methods: Taking the dry extract yield, the transfer rate of geniposide, baicalin and its anti-tumor and anti-inflammatory activities as evaluation indexes, the extraction process of Compound Zhizi Jinhua was investigated by Plackett-Burnman combined with Box-Behnken design, and the optimum extraction parameters were determined. Results: The optimal extraction conditions were 44 times of 29% alcohol, reflux extraction 3 times, 3 h each time. Conclusion: The extraction process determined in this study was reasonable and feasible, which could shorten the experiment period, significantly improve the transfer rate of active ingredients, and combined with the activity evaluation, the screening results were more instructive.
Objective: To optimize the extraction process conditions of Compound Zhizi Jinhua. Methods: Taking the dry extract yield, the transfer rate of geniposide, baicalin and its anti-tumor and anti-inflammatory activities as evaluation indexes, the extraction process of Compound Zhizi Jinhua was investigated by Plackett-Burnman combined with Box-Behnken design, and the optimum extraction parameters were determined. Results: The optimal extraction conditions were 44 times of 29% alcohol, reflux extraction 3 times, 3 h each time. Conclusion: The extraction process determined in this study was reasonable and feasible, which could shorten the experiment period, significantly improve the transfer rate of active ingredients, and combined with the activity evaluation, the screening results were more instructive.
引文
[1]国家药典委员会.中华人民共和国药典.一部.北京:中国医药科技出版社,2015
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[3]陈庄元,董立莎,曹佩雪,等.不同栀子金花汤煎液中3种成分的变化及抑菌作用的比较.华西药学杂志,2008,23(2):135-137
[4]王鸣,舒志军,张胜华,等.栀子金花汤对腹腔感染脓毒症大鼠炎症介质的影响.中国中西医结合急救杂志,2007,14(3):169-172
[5]刘俐伶,麻继臣,甄晓静.栀子金花丸治疗面部糖皮质激素依赖性皮炎临床观察.中国中西医结合皮肤性病学杂志,2009,8(3):175
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[8]高宁,张婷婷,张薇,等.Plackett-Burman设计和响应面法优化黑果小檗果实总黄酮超声提取工艺.新疆医科大学学报,2017,40(4):494-497
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[10]王永香,米慧娟,张传力,等.Box-Behnken响应面法优化热毒宁注射液金银花和青蒿(金青)的醇沉工艺研究.中草药,2015,46(5):671-678
[11]叶陈丽,贺帅,康迎波,等.Box-Behnken效应面法优化金翘热毒清复方中绿原酸和连翘酯苷A的提取工艺.中国药学杂志,2014,49(7):543-549
[12]马翠霞,赵武,肖凤琴,等Box-Behnken响应面法优化小飞蓬总黄酮纯化工艺及其抗肿瘤活性研究.中华中医药杂志,2018,33(9):4104-4109
[13]张艳军,刘莉莉,胡军华,等.基于Plackett-Burman设计和BoxBehnken响应面法优化鼻鼽颗粒一步制粒工艺.中国中药杂志,2015,40(22):4406-4410
[14]宋佳,黄飞龙,段树卿,等.Plackett-Burnman设计联合BoxBehnken设计-效应面法优化心神宁片的提取工艺.中草药,2016,47(3):430-435
[15]林彦君,游宇,吴品江.Plackett-Burman与Box-Behnken设计-效应面法优化射干总黄酮磷脂复合物制备工艺研究.成都中医药大学学报,2015,38(1):44-48
[16]罗俊,尚海,成伟华,等.西松烷型二萜衍生物的合成及抗肿瘤活性研究.中草药,2017,48(3):460-467
[17]Yang B Y,Guo R,Li T,et al.New anti-inflammatory withanolides from the leaves of Datura metel L..Steroids,2014,87(13):26-34
[18]Yang B Y,Xia Y G,Liu Y,et al.New antiproliferative and immunosuppressive withanolides from the seeds of Datura metel.Phytochemistry Letters,2014,8(5):92-96
[2]牛德斌.HPLC测定栀子金花丸中2类成分的含量.中国实验方剂学杂志,2011,17(16):96-97
[3]陈庄元,董立莎,曹佩雪,等.不同栀子金花汤煎液中3种成分的变化及抑菌作用的比较.华西药学杂志,2008,23(2):135-137
[4]王鸣,舒志军,张胜华,等.栀子金花汤对腹腔感染脓毒症大鼠炎症介质的影响.中国中西医结合急救杂志,2007,14(3):169-172
[5]刘俐伶,麻继臣,甄晓静.栀子金花丸治疗面部糖皮质激素依赖性皮炎临床观察.中国中西医结合皮肤性病学杂志,2009,8(3):175
[6]孙晔.春季上火分清虚实.中国中医药报,2011-2-25(006)
[7]艾志福,伍振峰,张帅杰,等.Plackett-Burman设计结合响应面法优化天麻效应成分的提取工艺.中国实验方剂学杂志,2014,20(14):34-37
[8]高宁,张婷婷,张薇,等.Plackett-Burman设计和响应面法优化黑果小檗果实总黄酮超声提取工艺.新疆医科大学学报,2017,40(4):494-497
[9]伍振峰,陈伟良,王雅琪,等.丹参减压提取工艺优化及技术适宜性研究.中草药,2014,45(6):795-800
[10]王永香,米慧娟,张传力,等.Box-Behnken响应面法优化热毒宁注射液金银花和青蒿(金青)的醇沉工艺研究.中草药,2015,46(5):671-678
[11]叶陈丽,贺帅,康迎波,等.Box-Behnken效应面法优化金翘热毒清复方中绿原酸和连翘酯苷A的提取工艺.中国药学杂志,2014,49(7):543-549
[12]马翠霞,赵武,肖凤琴,等Box-Behnken响应面法优化小飞蓬总黄酮纯化工艺及其抗肿瘤活性研究.中华中医药杂志,2018,33(9):4104-4109
[13]张艳军,刘莉莉,胡军华,等.基于Plackett-Burman设计和BoxBehnken响应面法优化鼻鼽颗粒一步制粒工艺.中国中药杂志,2015,40(22):4406-4410
[14]宋佳,黄飞龙,段树卿,等.Plackett-Burnman设计联合BoxBehnken设计-效应面法优化心神宁片的提取工艺.中草药,2016,47(3):430-435
[15]林彦君,游宇,吴品江.Plackett-Burman与Box-Behnken设计-效应面法优化射干总黄酮磷脂复合物制备工艺研究.成都中医药大学学报,2015,38(1):44-48
[16]罗俊,尚海,成伟华,等.西松烷型二萜衍生物的合成及抗肿瘤活性研究.中草药,2017,48(3):460-467
[17]Yang B Y,Guo R,Li T,et al.New anti-inflammatory withanolides from the leaves of Datura metel L..Steroids,2014,87(13):26-34
[18]Yang B Y,Xia Y G,Liu Y,et al.New antiproliferative and immunosuppressive withanolides from the seeds of Datura metel.Phytochemistry Letters,2014,8(5):92-96
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