lncRNA-linc00152在口腔鳞状细胞癌组织、癌旁组织及口腔鳞癌细胞系中的表达及作用机制
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摘要
目的分析长链非编码RNA(lncRNA)-linc00152在口腔鳞状细胞癌(OSCC)组织、正常癌旁组织及OSCC细胞系中的表达及作用机制。方法回顾性收集西安交通大学第一附属医院的44例OSCC组织标本及其相应的正常癌旁组织标本。通过实时荧光定量PCR法对OSCC组织、正常癌旁组织及OSCC细胞系中的linc00152表达水平进行检测,并比较不同临床病理特征的OSCC患者linc00152表达水平的差异。采用SCC9与CAL27细胞对linc00152-siRNA进行转染,分别采用CCK-8、Transwell和划痕实验对SCC9与CAL27细胞增殖、侵袭及迁移情况进行检测。结果相比正常癌旁组织,OSCC组织标本中的linc00152表达水平明显上调(P <0.01)。相比正常黏膜HOK细胞,OSCC细胞系SCC9、SCC15、CAL27及TSCCA细胞中的linc00152表达水平明显上调(P <0.01),而linc00152在不同性别、年龄、TNM分期及是否伴有局部浸润患者中表达水平的比较,均无统计学差异(P> 0.05)。伴有淋巴结转移者linc00152表达水平较无淋巴结转移者明显上调(P <0.05)。细胞增殖实验结果发现,采用linc00152-sinRNA敲低SCC9与CAL27细胞中的linc00152表达后,可明显阻滞细胞生长速率(P <0.05)。细胞侵袭实验结果发现,下调linc00152表达后,OSCC细胞系SCC9与CAL27细胞侵袭能力较对照组明显下降(P <0.05)。细胞迁移实验结果发现,下调linc00152表达后,OSCC细胞系SCC9与CAL27细胞迁移能力较对照组明显下降(P <0.05)。结论 linc00152高表达于OSCC组织与OSCC细胞系,采用linc00152-sinRNA敲低SCC9与CAL27细胞中的linc00152表达后,可有效阻滞OSCC细胞增殖、侵袭及迁移,提示linc00152有望成为临床治疗OSCC的潜在靶点。
Objective To analyze the expression and mechanism of long-chain non-coding RNA(lncRNA)-linc00152 in oral squamous cell carcinoma(OSCC),normal adjacent tissues and OSCC cell lines.Methods The expression levels of linc00152 in OSCC tissues,normal para-cancerous tissues and OSCC cell lines were detected by real-time fluorescence quantitative PCR,and the differences of linc00152 expression levels among OSCC patients with different clinicopathological characteristics were compared.SCC9 and CAL27 cells were transfected with linc00152-siRNA.CCK-8.Transwell and scratch test were used to detect the proliferation,invasion and migration of SCC9 and CAL27 cells.Results The expression of linc00152 in OSCC tissues was significantly higher than that in normal adjacent tissues(P < 0.01).Compared with normal HOK cells,the expression of linc00152 in OSCC cell lines SCC9,SCC15,CAL27 and TSCCA cells was significantly increased(P <0.01),while the expression of linc00152 in patients with different gender,ages,TNM stage and local infiltration was not significantly different(P > 0.05).The expression of linc00152 in patients with lymph node metastasis was significantly higher than that in patients without lymph node metastasis(P < 0.05).The results of cell proliferation experiment showed that the growth rate of SCC9 and CAL27 cells was significantly blocked by knocking down the expression of linc00152 in SCC9 and CAL27 cells with linc00152-sinRNA(P < 0.05).The results of cell invasion experiment showed that the invasive ability of OSCC cell lines SCC9 and CAL27 decreased significantly after down-regulating the expression of linc00152(P < 0.05).The results of cell migration experiment showed that the migration ability of OSCC cell lines SCC9 and CAL27 decreased significantly after down-regulation of linc00152 expression(P < 0.05).Conclusion Linc00152 is highly expressed in OSCC tissues and OSCC cell lines.When linc00152-sinRNA is used to knock down the expression of linc00152 in SCC9 and CAL27 cells,it can effectively block the proliferation,invasion and migration of OSCC cells,suggesting that linc00152 may be a potential target for clinical treatment of OSCC.
Objective To analyze the expression and mechanism of long-chain non-coding RNA(lncRNA)-linc00152 in oral squamous cell carcinoma(OSCC),normal adjacent tissues and OSCC cell lines.Methods The expression levels of linc00152 in OSCC tissues,normal para-cancerous tissues and OSCC cell lines were detected by real-time fluorescence quantitative PCR,and the differences of linc00152 expression levels among OSCC patients with different clinicopathological characteristics were compared.SCC9 and CAL27 cells were transfected with linc00152-siRNA.CCK-8.Transwell and scratch test were used to detect the proliferation,invasion and migration of SCC9 and CAL27 cells.Results The expression of linc00152 in OSCC tissues was significantly higher than that in normal adjacent tissues(P < 0.01).Compared with normal HOK cells,the expression of linc00152 in OSCC cell lines SCC9,SCC15,CAL27 and TSCCA cells was significantly increased(P <0.01),while the expression of linc00152 in patients with different gender,ages,TNM stage and local infiltration was not significantly different(P > 0.05).The expression of linc00152 in patients with lymph node metastasis was significantly higher than that in patients without lymph node metastasis(P < 0.05).The results of cell proliferation experiment showed that the growth rate of SCC9 and CAL27 cells was significantly blocked by knocking down the expression of linc00152 in SCC9 and CAL27 cells with linc00152-sinRNA(P < 0.05).The results of cell invasion experiment showed that the invasive ability of OSCC cell lines SCC9 and CAL27 decreased significantly after down-regulating the expression of linc00152(P < 0.05).The results of cell migration experiment showed that the migration ability of OSCC cell lines SCC9 and CAL27 decreased significantly after down-regulation of linc00152 expression(P < 0.05).Conclusion Linc00152 is highly expressed in OSCC tissues and OSCC cell lines.When linc00152-sinRNA is used to knock down the expression of linc00152 in SCC9 and CAL27 cells,it can effectively block the proliferation,invasion and migration of OSCC cells,suggesting that linc00152 may be a potential target for clinical treatment of OSCC.
引文
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[14]Cai Q,Wang ZQ,Wang SH,et al.Upregulation of long non-coding RNA LINC00152 by SP1 contributes to gallbladder cancer cell growth and tumor metastasis via PI3K/AKT pathway[J].Am J Transl Res,2016,8(10):4068-4081.
[15]Sun Z,Guo X,Zang M,et al.Long non-coding RNA LINC00152promotes cell growth and invasion of papillary thyroid carcinoma by regulating the miR-497/BDNF axis[J].J Cell Physiol,2019,234(2):1336-1345.
[2]Yao LX,Liu J,Xu L.MiR-610 functions as a tumor suppressor in oral squamous cell carcinoma by directly targeting AGK[J].Eur Rev Med Pharmacol Sci,2019,23(1):187-197.
[3]Haque SU,Niu L,Kuhnell D,et al.Differential expression and prognostic value of long non-coding RNA in HPV-negative head and neck squamous cell carcinoma[J].Head Neck,2018,40(7):1555-1564.
[4]郭春芳,李华强,陈晏林,等.卵巢癌患者组织中lncRNA NEAT1和CTBP2表达水平及其与临床病理特征的关系[J].临床和实验医学杂志,2018,17(12):1285-1289.
[5]王玉佩,冷卫东,牛玉明,等.OSCC组织特征性lncRNA表达谱及lncRNA CCAT2在口腔鳞状细胞癌发生发展中的作用[J].临床和实验医学杂志,2018,17(19):2089-2092.
[6]Xu MD,Qi P,Du X,et al.Long non-coding RNAs in colorectal cancer:implications for pathogenesis and clinical application[J].Mod Pathol,2014,27(10):1310-1320.
[7]Weidle UH,Birzele F,Kollmorgen G,et al.Long non-coding RNAs and their role in metastasis[J].Cancer Genomics Proteomics,2017,14(3):143-160.
[8]Sun W,Yang Y,Xu C,et al.Regulatory mechanisms of long noncoding RNAs on gene expression in cancers[J].Cancer Genet,2017,216-217(1):105-110.
[9]Hu XL,Wang J,He W,et al.Down-regulation of lncRNA Linc00152suppressed cell viability,invasion,migration,and epithelial to mesenchymal transition,and reversed chemo-resistance in breast cancer cells[J].Eur Rev Med Pharmacol Sci,2018,22(10):3074-3084.
[10]Nishizawa Y,Konno M,Asai A,et al.Hypoxia stimulates the cytoplasmic localization of oncogenic long noncoding RNA LINC00152 in colorectal cancer[J].Int J Oncol,2018,52(2):453-460.
[11]Wang Y,Liu J,Bai H,et al.Long intergenic non-coding RNA 00152promotes renal cell carcinoma progression by epigenetically suppressing P16 and negatively regulates miR-205[J].Am J Cancer Res,2017,7(2):312-322.
[12]Mao Y,Tie Y,Du J,et al.LINC00152 promotes the proliferation of gastric cancer cells by regulating B-cell lymphoma-2[J].J Cell Biochem,2019,120(3):3747-3756.
[13]Deng X,Zhao XF,Liang XQ,et al.Linc00152 promotes cancer progression in hepatitis B virus-associated hepatocellular carcinoma[J].Biomed Pharmacother,2017,90(1):100-108.
[14]Cai Q,Wang ZQ,Wang SH,et al.Upregulation of long non-coding RNA LINC00152 by SP1 contributes to gallbladder cancer cell growth and tumor metastasis via PI3K/AKT pathway[J].Am J Transl Res,2016,8(10):4068-4081.
[15]Sun Z,Guo X,Zang M,et al.Long non-coding RNA LINC00152promotes cell growth and invasion of papillary thyroid carcinoma by regulating the miR-497/BDNF axis[J].J Cell Physiol,2019,234(2):1336-1345.
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