不同产地海地瓜活性物质检测及降糖活性评价
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摘要
目的通过不同产地海地瓜活性物质的检测及降糖活性评价,为海地瓜的高值化利用提供理论依据。方法运用凯氏定氮法测定粗蛋白含量、硫酸-苯酚法测定多糖含量、比色法测定皂苷含量、溶剂萃取法测定油脂含量,采用α-淀粉酶抑制剂筛选方法测定活性物质的体外降糖活性。采用单因素方差分析、主成分分析(PCA)、正交偏最小二乘判别分析(OPLS-DA)方法对数据进行分析。结果不同产地海地瓜活性物质在含量与活性两方面均存在差异,且越南(CD1)、广东(CD3)、福建(CD4)、海南(CD8)与浙江(CD2)、威海(CD5)、烟台(CD6)、日照(CD7)分类明显,而对分类效果贡献最大的是皂苷、多糖、油脂的降糖活性。结论本研究对海地瓜中活性物质的挖掘具有一定的指导意义,可精准选择合适产地的海地瓜,快速、高效分离纯化海地瓜中的活性物质,实现其高值化利用。
Objective Through the detection of active substances and the evaluation of hypoglycemic activity in acaudina molpadioides from different producing areas,the next step of separation and purification of active substances in acaudina molpadioides is guided.Methods Determination of crude protein content by Kjeldahl method,determination of polysaccharide content by sulfuric acid-phenol method,determination of saponin content by colorimetric method,determination of lipa content by solvent extraction method,determination lipa hypoglycemic activity of active substance in vitro byα-amylase inhibitor screening method.The data were analyzed by one-way analysis of variance,principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA).Results There were differences in the content and activity of the active substances in different producing areas.Yuenan(CD1)、Guangdong(CD3)、Fujian(CD4)、Hainan(CD8)and Zhejiang(CD2)、Weihai(CD5)、Yantai(CD6)、Rizhao(CD7)were clearly classified,and the most important contribution to the classification effect was the hypoglycemic activity of saponins,polysaccharides and lipa.Conclusion This study has certain guiding significance for the excavation of active substances in acaudina molpadioides.In the later research work,it was expected to accurately select the suitable producing area of the acaudina molpadioides to separate and purify the active substances quickly and efficiently.
Objective Through the detection of active substances and the evaluation of hypoglycemic activity in acaudina molpadioides from different producing areas,the next step of separation and purification of active substances in acaudina molpadioides is guided.Methods Determination of crude protein content by Kjeldahl method,determination of polysaccharide content by sulfuric acid-phenol method,determination of saponin content by colorimetric method,determination of lipa content by solvent extraction method,determination lipa hypoglycemic activity of active substance in vitro byα-amylase inhibitor screening method.The data were analyzed by one-way analysis of variance,principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA).Results There were differences in the content and activity of the active substances in different producing areas.Yuenan(CD1)、Guangdong(CD3)、Fujian(CD4)、Hainan(CD8)and Zhejiang(CD2)、Weihai(CD5)、Yantai(CD6)、Rizhao(CD7)were clearly classified,and the most important contribution to the classification effect was the hypoglycemic activity of saponins,polysaccharides and lipa.Conclusion This study has certain guiding significance for the excavation of active substances in acaudina molpadioides.In the later research work,it was expected to accurately select the suitable producing area of the acaudina molpadioides to separate and purify the active substances quickly and efficiently.
引文
[1]王宁丽,魏鉴腾,王勇,等.海地瓜血管紧张素转换酶抑制肽体外评价[J].食品科技,2017,42(9):224-226.
[2]苏永昌,刘淑集,刘秋凤,等.海地瓜营养成分的分析及评价[J].渔业研究,2016,38(4):288-294.
[3]陈发河,李真,吴光斌.海地瓜多糖和多肽提取纯化工艺及抗氧化活性[J].集美大学学报(自然科学版),2018,23(2):105-118.
[4]Wang Y,Su W,Zhang C,et al.Protective effect of sea cucumber(acaudina molpadioides)fucoidan against ethanolinduced gastric damage[J].Food Chem,2012,133(4):1414-1419.
[5]阮伟达.海地瓜总皂苷提取纯化工艺及生物活性研究[D].厦门:集美大学,2012.
[6]冯婷玉.海地瓜(acaudina molpadioides)活性脂质的制备研究及抗肿瘤活性初探[D].青岛:中国海洋大学,2011.
[7]董丽莎,李妍妍,张红燕,等.利用基因芯片技术解析海地瓜酶解液对糖尿病小鼠糖脂代谢的调控[J].食品科学,2018,39(9):145-153.
[8]王宁丽,魏鉴腾,祝潇,等.基于碘-淀粉显色的α-淀粉酶抑制剂筛选方法研究及应用[J].中国海洋药物,2017,36(5):75-78.
[9]Du L,Li Z J,Xu J,et al.The anti-tumor activities of cerebrosides derived from sea cucumber acaudina molpadioides and starfish Asterias amurensis in vitro and in vivo[J].JOleo Sci,2012,61(6):321-330.
[10]黄丽英,范栋杰,张月星,等.元素含量及稳定同位素比值用于网销带鱼产地溯源[J/OL].分析化学:1-12[2019-01-14].https://doi.org/10.19756/j.issn.0253-3820.181422.
[11]邹士玉,马方励,丁博.苯酚-硫酸法测定“润和津露”的粗多糖含量[J].中药材,2007,30(11):1470-1472.
[12]毕云枫,王溪竹,姜珊,等.不同人参皂苷为标准品测定皂苷含量的比较研究[J].食品工业,2018,39(8):200-203.
[13]董平,薛长湖,盛文静,等.海参中总皂苷含量测定方法的研究[J].中国海洋药物,2008,27(1):28-32.
[14]林汝榕,蔡文旋,柯秀蓉,等.采用加速溶剂萃取法提高微藻油脂萃取量的优化条件研究[J].台湾海峡,2011,30(3):437-443.
[2]苏永昌,刘淑集,刘秋凤,等.海地瓜营养成分的分析及评价[J].渔业研究,2016,38(4):288-294.
[3]陈发河,李真,吴光斌.海地瓜多糖和多肽提取纯化工艺及抗氧化活性[J].集美大学学报(自然科学版),2018,23(2):105-118.
[4]Wang Y,Su W,Zhang C,et al.Protective effect of sea cucumber(acaudina molpadioides)fucoidan against ethanolinduced gastric damage[J].Food Chem,2012,133(4):1414-1419.
[5]阮伟达.海地瓜总皂苷提取纯化工艺及生物活性研究[D].厦门:集美大学,2012.
[6]冯婷玉.海地瓜(acaudina molpadioides)活性脂质的制备研究及抗肿瘤活性初探[D].青岛:中国海洋大学,2011.
[7]董丽莎,李妍妍,张红燕,等.利用基因芯片技术解析海地瓜酶解液对糖尿病小鼠糖脂代谢的调控[J].食品科学,2018,39(9):145-153.
[8]王宁丽,魏鉴腾,祝潇,等.基于碘-淀粉显色的α-淀粉酶抑制剂筛选方法研究及应用[J].中国海洋药物,2017,36(5):75-78.
[9]Du L,Li Z J,Xu J,et al.The anti-tumor activities of cerebrosides derived from sea cucumber acaudina molpadioides and starfish Asterias amurensis in vitro and in vivo[J].JOleo Sci,2012,61(6):321-330.
[10]黄丽英,范栋杰,张月星,等.元素含量及稳定同位素比值用于网销带鱼产地溯源[J/OL].分析化学:1-12[2019-01-14].https://doi.org/10.19756/j.issn.0253-3820.181422.
[11]邹士玉,马方励,丁博.苯酚-硫酸法测定“润和津露”的粗多糖含量[J].中药材,2007,30(11):1470-1472.
[12]毕云枫,王溪竹,姜珊,等.不同人参皂苷为标准品测定皂苷含量的比较研究[J].食品工业,2018,39(8):200-203.
[13]董平,薛长湖,盛文静,等.海参中总皂苷含量测定方法的研究[J].中国海洋药物,2008,27(1):28-32.
[14]林汝榕,蔡文旋,柯秀蓉,等.采用加速溶剂萃取法提高微藻油脂萃取量的优化条件研究[J].台湾海峡,2011,30(3):437-443.
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