电针“太冲”“百会”对自发性高血压大鼠肾组织ACE2、Ang(1-7)表达及Ang(1-7)/AngⅡ比值的影响
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摘要
目的:观察电针"太冲""百会"对自发性高血压大鼠(SHR)肾RAS系统中舒血管活性因子的影响,探讨其对高血压肾损害的保护机制。方法:24只雄性SHR大鼠随机分为电针组和模型组,12只雄性Wistar-Kyoto(WKY)大鼠作为空白组,电针组电针两极分别连接右侧"太冲穴"和"百会穴"(1 m A,2Hz/15 Hz,20 min)。观察各组血压变化,检测血清中尿酸(UA)和尿素氮(BUN)的含量、肾组织ACE2、Ang(1-7)、AngⅡ的表达,计算Ang(1-7)/AngⅡ的比值; HE、Masson染色和电子透射显微镜观察肾脏结构形态变化。结果:电针组和模型组相比,收缩压、血清中UA、BUN的含量、AngⅡ表达均有所下降(P <0. 05);肾组织ACE2、Ang(1-7)的表达水平、Ang(1-7)/AngⅡ比值提高(P <0. 05)。HE、Masson染色以及电镜观察结果均显示电针组较模型组肾损害现象减轻。结论:电针"太冲""百会"穴能降低血压,减轻高血压对肾的损害。
Objective: To observe the effect of electro-needling Taichong( LR3) and Baihui( GV20) on vasoactive factors of RASS in spontaneous hypertensive rats( SHRs),and to explore its underlying protective mechanism of renal injury in hypertension. Methods: 24 male SHRs were randomized into the model group and the electro-acupuncture( EA) group,another 12 Wistar-Kyoto( WKY) rats were taken as the blank control.Points chosen in the EA group were right LR3 and right GV20( 1 mA,2 Hz/15 Hz,20 min). After 30 days of the treatment,the changes of Bp were observed,serum levels of blood uric acid( UA) and blood urea nitaogen( BUN) were detected,renal expressions of ACE2,Ang( 1-7),and AngⅡ were determined,as well as Ang( 1-7)/AngⅡ was calculated. Pathological changes of renal tissues were observed under HE staning,Masson staining and electron microscope. Results: In EA group,systolic pressure( SP) and the serum contents of AngⅡ,UA and BUN were decreased compared to those in the model group( P < 0. 05). The expressions of ACE2,Ang( 1-7),and Ang( 1-7)/AngII were increased in the kidney in the EA group compared to those in the model group( P < 0. 05). The results of HE staining,Masson staining and electron microscope showed that the kidney damage was less in the EA group than that in the model group. Conclusion: Electro-needling LR3 and GV20 can effectively reduce SP and relieve the damage of high blood pressure to kidney.
Objective: To observe the effect of electro-needling Taichong( LR3) and Baihui( GV20) on vasoactive factors of RASS in spontaneous hypertensive rats( SHRs),and to explore its underlying protective mechanism of renal injury in hypertension. Methods: 24 male SHRs were randomized into the model group and the electro-acupuncture( EA) group,another 12 Wistar-Kyoto( WKY) rats were taken as the blank control.Points chosen in the EA group were right LR3 and right GV20( 1 mA,2 Hz/15 Hz,20 min). After 30 days of the treatment,the changes of Bp were observed,serum levels of blood uric acid( UA) and blood urea nitaogen( BUN) were detected,renal expressions of ACE2,Ang( 1-7),and AngⅡ were determined,as well as Ang( 1-7)/AngⅡ was calculated. Pathological changes of renal tissues were observed under HE staning,Masson staining and electron microscope. Results: In EA group,systolic pressure( SP) and the serum contents of AngⅡ,UA and BUN were decreased compared to those in the model group( P < 0. 05). The expressions of ACE2,Ang( 1-7),and Ang( 1-7)/AngII were increased in the kidney in the EA group compared to those in the model group( P < 0. 05). The results of HE staining,Masson staining and electron microscope showed that the kidney damage was less in the EA group than that in the model group. Conclusion: Electro-needling LR3 and GV20 can effectively reduce SP and relieve the damage of high blood pressure to kidney.
引文
[1]刘巍,熊兴江,王阶,等.高血压肾损害及其中医药防治进展[J].中国中药杂志,2014,39(1):14-19.
[2] Coelho MS,Lopes KL,Freitas RA,et al. High sucrose intake in rats is associated with increased ACE2 and angiotensin-(1-7)levels in the adipose tissue[J]. Regulatory Peptides,2010,162(1-3):61-67.
[3] Marcus Y,Shefer G,Sasson K,et al. Angiotensin 1-7 as Means to Prevent the Metabolic Syndrome:Lessons From the Fructose-Fed Rat Model[J]. Diabetes,2013,62(4):561-570.
[4] Passos-Silva DG,Verano-Braga T,Santos RA. Angiotensin-(1-7):beyond the cardio-renal actions[J]. Clinical science,2013,124(7):443-456.
[5]宗文纳,卢新政.血管紧张素(1-7)/Mas的研究进展[J].国际心血管病杂志,2010,5(37):160.
[6]陈志娟,曲怡,刘宇,等.电针刺曲池、足三里穴对自发性高血压大鼠血压及肾功能的影响[J].中国中医急症,2012,12(9):1423-1424.
[7]陈玲,张智龙.针药并用治疗高血压病早期肾功能损害的临床研究[J].内蒙古中医药,2011,30(5):53-54.
[8]孙静文,田艳鹏,金娜美,等.捻转补泻手法对自发性高血压大鼠肾组织NO、NOS含量的影响[J].中华中医药杂志,2015,30(10):3684-3686.
[9]纪智,郭文,朱华超,等.以基因芯片探究针刺泻法对应激性高血压前期大鼠肾脏基因表达的影响[J].长春中医药大学学报,2016,32(3):458-461.
[10]田艳鹏,王朝阳,孙静文,等.针刺捻转补泻手法对自发性高血压大鼠RAS的影响[J].世界中医药,2015,10(7):1062-1066.
[11]林文注,王佩.实验针灸学[M].上海:科学技术出版社,1999:55.
[12]万斌,孙丽薇,刘蓉,等.电针对2型糖尿病合并肾性高血压大鼠模型的疗效[J].中国实验动物学报,2016,24(6):622-627.
[13]刘海华,王莹莹,高海波,等.针灸治疗高血压病选穴规律文献研究[J].中医杂志,2014,55(12):1055-1058.
[14]吴雪梅,李冰,吴宝,等.百会透刺前顶对1、2级原发性高血压动态血压的影响[J].北京中医药,2015,34(12):931-935.
[15]傅秋彤.百会放血治疗高血压病45例临床观察[J].北京中医,2001,20(2):44.
[16] Schillaci G,Pirro M,Mannarino MR,et al. Relation between renal function within the normal range and central and peripheral arterial stiffness in hypertension[J]. Hypertension,2006,48(4):111-120.
[17]张年宝,程慧珍,崔卫东,等.葛根素对肾性高血压大鼠的降压作用及对肾组织ANGⅡ的影响[J].中药药理与临床,2010,26(2):26-29.
[18]陈建,曾莉,陈刚,等.单侧肾切除及微渗透泵灌注血管紧张素Ⅱ诱导小鼠肾纤维化与高血压模型的建立[J].中国比较医学杂志,2015,25(2):26.
[19]金海燕,宋蓓,钟久昌,等. ACE2基因:高血压防治新的靶点[J/CD].中华临床医师杂志:电子版,2011,5(9):102-104.
[20]蒋毅弘,钟久昌.血管紧张素转换酶2、氧化应激与高血压[J].中华高血压杂志,2011,19(6):518-520.
[2] Coelho MS,Lopes KL,Freitas RA,et al. High sucrose intake in rats is associated with increased ACE2 and angiotensin-(1-7)levels in the adipose tissue[J]. Regulatory Peptides,2010,162(1-3):61-67.
[3] Marcus Y,Shefer G,Sasson K,et al. Angiotensin 1-7 as Means to Prevent the Metabolic Syndrome:Lessons From the Fructose-Fed Rat Model[J]. Diabetes,2013,62(4):561-570.
[4] Passos-Silva DG,Verano-Braga T,Santos RA. Angiotensin-(1-7):beyond the cardio-renal actions[J]. Clinical science,2013,124(7):443-456.
[5]宗文纳,卢新政.血管紧张素(1-7)/Mas的研究进展[J].国际心血管病杂志,2010,5(37):160.
[6]陈志娟,曲怡,刘宇,等.电针刺曲池、足三里穴对自发性高血压大鼠血压及肾功能的影响[J].中国中医急症,2012,12(9):1423-1424.
[7]陈玲,张智龙.针药并用治疗高血压病早期肾功能损害的临床研究[J].内蒙古中医药,2011,30(5):53-54.
[8]孙静文,田艳鹏,金娜美,等.捻转补泻手法对自发性高血压大鼠肾组织NO、NOS含量的影响[J].中华中医药杂志,2015,30(10):3684-3686.
[9]纪智,郭文,朱华超,等.以基因芯片探究针刺泻法对应激性高血压前期大鼠肾脏基因表达的影响[J].长春中医药大学学报,2016,32(3):458-461.
[10]田艳鹏,王朝阳,孙静文,等.针刺捻转补泻手法对自发性高血压大鼠RAS的影响[J].世界中医药,2015,10(7):1062-1066.
[11]林文注,王佩.实验针灸学[M].上海:科学技术出版社,1999:55.
[12]万斌,孙丽薇,刘蓉,等.电针对2型糖尿病合并肾性高血压大鼠模型的疗效[J].中国实验动物学报,2016,24(6):622-627.
[13]刘海华,王莹莹,高海波,等.针灸治疗高血压病选穴规律文献研究[J].中医杂志,2014,55(12):1055-1058.
[14]吴雪梅,李冰,吴宝,等.百会透刺前顶对1、2级原发性高血压动态血压的影响[J].北京中医药,2015,34(12):931-935.
[15]傅秋彤.百会放血治疗高血压病45例临床观察[J].北京中医,2001,20(2):44.
[16] Schillaci G,Pirro M,Mannarino MR,et al. Relation between renal function within the normal range and central and peripheral arterial stiffness in hypertension[J]. Hypertension,2006,48(4):111-120.
[17]张年宝,程慧珍,崔卫东,等.葛根素对肾性高血压大鼠的降压作用及对肾组织ANGⅡ的影响[J].中药药理与临床,2010,26(2):26-29.
[18]陈建,曾莉,陈刚,等.单侧肾切除及微渗透泵灌注血管紧张素Ⅱ诱导小鼠肾纤维化与高血压模型的建立[J].中国比较医学杂志,2015,25(2):26.
[19]金海燕,宋蓓,钟久昌,等. ACE2基因:高血压防治新的靶点[J/CD].中华临床医师杂志:电子版,2011,5(9):102-104.
[20]蒋毅弘,钟久昌.血管紧张素转换酶2、氧化应激与高血压[J].中华高血压杂志,2011,19(6):518-520.
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