不同大白菜材料对根肿菌生理小种的抗性反应及抗病位点检测
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摘要
为了明确主栽的大白菜以及大白菜育种材料与根肿菌生理小种之间的抗性关系,以现有4,7,10和11号生理小种为菌源,用注射法进行接种,对25份供试大白菜材料进行抗病性鉴定,同时利用分子标记检测供试材料中所含的根肿病抗性位点。结果表明:供试大白菜中,山地王2号等8份试材对4个生理小种都表现抗病,德高CR117等7份试材对4个生理小种都表现感病,C1等4份试材对2个生理小种感病,华抗301等6份试材只对4号生理小种感病;所有试材均含有Crr1抗病位点,德高CR117等11份试材含有Crr2抗病位点,仅在京春CR3中检测到Crr3抗病位点,除德高CR铁甲1号和大地CR118外,其他试材中均检测到了CRk抗病位点,有21,16份试材中分别检测到抗病位点CRa和CRb,而CRc抗病位点仅在星光和CR75中存在。总之,不同大白菜材料对同一生理小种抗性反应不同,同一个材料对不同生理小种的抗性反应不同;所有生理小种中4号生理小种最强,其次为7号生理小种;大白菜材料抗病位点多一定抗病性强;Crr1抗病位点对材料抗性影响不大。
To clarify the resistant relationship between physiological races and Chinese cabbage(Brassica rapa L.ssp. pekinensis) variety, physiological races 4, 7, 10 and 11 were inoculated by injecting. The disease resistance of the 25 Chinese cabbage and CR markers were detected. The results showed that 8 cultivars such as Shandiwang 2 were resistance to only 4 physiological races; 7 cultivars such as Degao CR117 were susceptible to only 4 physiological races; 4 cultivars(including C1) were susceptible to 2 physiological races, and 6 cultivars(including Huakang 301) were only susceptible to 4 physiological race. All materials contained Crr1, CRk was detected in samples except the Degao CR Tiejia 1 and the Dadi CR118, the disease resistance locus CRa and CRb were detected in 21 and 16 samples respectively, 11 cultivars(including Degao CR117) contained Crr2 resistance locus, Crr3 resistance locus was detected only in Jingchun CR3, CRc resistance locus was contained in Xingguan and CR75.In short,different Chinese cabbage materials have different resistance to the same physiological race,and the same material responds differently to different physiological races.The physiological race No.4 was the most serious, followed by the physiological race No.7. The results of molecular marker identification showed that the resistance sites of Chinese cabbage materials were not necessarily resistant to disease. The resistance of Crr1 had little effect on material resistance.
To clarify the resistant relationship between physiological races and Chinese cabbage(Brassica rapa L.ssp. pekinensis) variety, physiological races 4, 7, 10 and 11 were inoculated by injecting. The disease resistance of the 25 Chinese cabbage and CR markers were detected. The results showed that 8 cultivars such as Shandiwang 2 were resistance to only 4 physiological races; 7 cultivars such as Degao CR117 were susceptible to only 4 physiological races; 4 cultivars(including C1) were susceptible to 2 physiological races, and 6 cultivars(including Huakang 301) were only susceptible to 4 physiological race. All materials contained Crr1, CRk was detected in samples except the Degao CR Tiejia 1 and the Dadi CR118, the disease resistance locus CRa and CRb were detected in 21 and 16 samples respectively, 11 cultivars(including Degao CR117) contained Crr2 resistance locus, Crr3 resistance locus was detected only in Jingchun CR3, CRc resistance locus was contained in Xingguan and CR75.In short,different Chinese cabbage materials have different resistance to the same physiological race,and the same material responds differently to different physiological races.The physiological race No.4 was the most serious, followed by the physiological race No.7. The results of molecular marker identification showed that the resistance sites of Chinese cabbage materials were not necessarily resistant to disease. The resistance of Crr1 had little effect on material resistance.
引文
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[2] Schwelm A,Fogelqvist J,Knaust A,Jülke S,Lilja T,Bonilla-Rosso G,Karlsson M,Shevchenko A,Dhandapani V,Choi S R,Kim H G,Park J Y,Lim Y P,Ludwig-Müller J,Dixeliusl C.The Plasmodiophora brassicae genome reveals insights in its life cycle and ancestry of chitin synthases[J].Scientific Reports,2015,5:11153.doi:10.1038/srep11153.
[3] Elke D,Martin F,Enrico L G A,Katsunori H,Masashi H.Status and perspectives of clubroot resistance breeding in crucifer crops[J].Plant Growth Regul,2009,28:265-281.doi:10.1103/PhysRevB.56.12490.
[4] 原玉香,赵艳艳,魏小春,姚秋菊,蒋武生,王志勇,李扬,许茜,杨双娟,张晓伟.河南省大白菜根肿病菌生理小种鉴定[J].河南农业科学,2017,46(7):71-76.doi:10.15933/j.cnki.1004-3268.2017.07.012.Yuan Y X,ZhaoY Y,Wei X C,Yao Q J,Jiang W S,Wang Z Y,Li Y,Xu Q,Yang S J,Zhang X W.Pathotype identification of Plasmodiophora brassicae woron.collected from Chinese cabbage in Henan Province[J].Journal of Henan Agricultural Sciences,2017,46(7):71-76.
[5] 汪维红,余阳俊,丁云花,张凤兰,于拴仓,张德双,赵岫云,卢桂香.湖北省长阳县十字花科蔬菜根肿病菌生理小种鉴定及抗源筛选[J].中国蔬菜,2013(12):55-60.Wang W H,Yu Y J,Ding Y H,Zhang F L,Yu S C,Zhang D S,Zhao X Y,Lu G X.Physiological race identification of clubroot (Plasmodiphora braassicae Wor.) from Changyang county,Hubei Province and resistant resource screening for Chinese cabbage breeding[J].China Vegetables,2013(12):55-60.
[6] 赵杨,白元俊,苗则彦,李颖,赵奎华.东北地区大白菜根肿病菌生理小种鉴定[J].湖南农业大学学报(自然科学版),2013,39(2):176-178.doi:1007-1032 (2013) 02-0176-03.Zhao Y,Bai Y J,Miao Z Y,Li Y,Zhao K H.Identification of physiological races of Plasmodiophora brassicae causing club root in Chinese cabbage from Northeast China[J].Journal of Hunan Agricultural University (Natural Sciences),2013,39(2):176-178.
[7] 沈向群,聂凯,吴琼,张玉光,孟星河.大白菜根肿病主要生理小种种群分化鉴定初报[J].中国蔬菜,2009 (8):59-62.Shen X Q,Nie K,Wu Q,Zhang Y G,Meng X G.Initial research report on differentiation identification of Chinese cabbage clubroot main physiological races[J].China Vegetables,2009 (8):59-62.
[8] 彭沙莎,任佐华,黄小莉,刘敏捷,孙良菲,刘二明.湖南十字花科作物根肿病菌生理小种鉴定[J].长江蔬菜,2013(6):46-49.doi:10.3865/j.issn.1001-3547.2013.06.019.Peng S S,Ren Z H,Huang X L,Liu M J,Sun L F,Liu E M.Identification of physiological races of Plasmodiophora brassicae causing club root in Chinese cabbage from Hunan Province[J].Joural of Changjiang Vegetables,2013(6):46-49.
[9] 刘峰,张丽辉,姬广海.云南和西藏十字花科蔬菜根肿病菌生理小种鉴定[J].中国蔬菜,2013(20):77-81.Liu F,Zhang L H,Ji G H.Identification of physiological race of Plasmodiophora brassicae in Yunnan and Tibet[J].China Vegatables,2013(20):77-81.
[10] 陈静,任雪松,宋洪元,李成琼,袁天成,司军.4个不同地区十字花科根肿病菌生理小种鉴定及甘蓝新组合的抗性鉴定[J].西南大学学报(自然科学版),2016,38(1):67-72.doi:10.13718/j.cnki.xdzk.2016.01.010.Chen J,Ren X S,Song H Y,Li C Q,Yuan T C,Si J.Identification of physiological races of Plasmodiophora brassicae in 4 different areas and identification of resistance in new combination of Brassica oleracea L.[J].Journal of Southwest University(Natural Sciences Edition),2016,38(1):67-72.
[11] Matsumoto E,Yasui C,Ohi M,Tsukada M.Linkage analysis of RFLP markers for clubroot resistance and pigmentation in Chinese cabbage (Brassica rapa ssp.Pekinensis) [J].Euphytica,1998.104:79-86.doi:10.1023/a:1018370418201.
[12] Hatakeyama K,Niwa T,Kato T,Ohara T,Kakizaki T,Matsumoto S.The tandem repeated organization of NB-LRR genes in the clubroot resistant CRb locus in Brassica rapa L.[J].Mol Genet Genom,2017,29(2):397-405.doi:10.1007/s00438-016-1281-1.
[13] Saito M N,Kubo S.Matsumoto K,Suwabe K,Tsukada M,Hirai M.Fine mapping of the clubroot resistance gene Crr3 in Brassica rapa[J].Theor Appl Genet,2006,114:81-91.doi:10.1007/s00122-006-0412-1.
[14] Sakamoto K,Saito A,Hayashida N,Taguchi G,Matsumoto E.Mapping of isolate-specific QTLs for clubroot resistance in Chinese cabbage(Brassica rapa L.ssp.Pekinensis) [J].Theor Appl Genet,2008,117:759-767.doi:10.1007/s00122-008-0817-0.
[15] Suwabe K,Tsukazaki H,Iketani H,Hatakeyama K,Fujimura M,Nunome T,Ukuoka H,Matsumoto S,Hirai M.Identification of two loci for resistance to clubroot(Plasmodiophora brassicae Woron.) in Brassica rapa L.[J].Theor Appl Genet,2003,107:997-1002.doi:10.1007/s00122-003-1309-x.
[16] Suwabe K,Tsukazaki H,Iketani H,Hatakeyama K,Kondo M,Fujimura M,Nunome T,Fukuoka H,Hirai M,Matsumoto S.Simple sequence repeat-based comparative genomics between Brassica rapa and Arabidopsis thaliana:the genetic origin of clubroot resistance[J].Genetics,2006,173:309-319.doi:10.1534/genetics.104.038968.
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[19] 曾令益,任莉,刘凡,陈旺,陈坤荣,徐理,方小平.28 个大白菜品种对根肿菌不同菌株的抗性反应及抗病基因位点检测[J].中国油料作物学报,2017,39(4):532-639.doi:10.7505/j issn.1007-9084.2017.04.015.Zeng L Y,Ren L,Liu F,Chen W,Chen K R,Xu L,Fang X P.Reaction and resistant gene loci detection of 28 Chinese cabbage cultivars to different Plasmodiophora brassicae strains[J].Chinese Journal of Oil Crop Sciences,2017,39(4):532-639.
[20] 王涛,王超楠,张红,温娟娟,张斌.大白菜基因组DNA快速提取方法的研究[J].华北农学报,2017,32(6):67-72.doi:10.7668/hbnxb.2017.06.010.Wang T,Wang C N,Zhang H,Wen J J,Zhang B.Study on rapid extraction of genomic DNA from Chinese cabbage[J].Acta Agriculturae Boreali-Sinica,2017,32(6):67-72.
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