雏番鸭细小病毒和传染性法氏囊病病毒混合感染的检测
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摘要
2017年12月,广西某番鸭养殖场的鸭群出现生长缓慢,临床表现软脚,拉白色粪便的腹泻并伴有一定的神经症状等;剖检可见肝脏肿大出血、腹腔有纤维素性渗出物、肠黏膜有不同程度的充血,法氏囊萎缩等病变;于20日龄开始发病,30日龄送检时发病率20%、死亡率10%。无菌取番鸭的病变组织,分别进行番鸭细小病毒(MDPV)和传染性法氏囊病病毒(IBDV)的核酸特异性检测,结果均呈阳性,并成功分离到一株番鸭细小病毒(命名为MDPV GX1712)和一株传染性法氏囊病病毒(命名为GL1712);基因序列分析结果显示,分离株GX1712与GX5、P 1988、 SAASSHNH等MDPV参考株的亲缘性最近,相似性高达98.5%～99.1%,在进化树中处同一个分支,并且与疫苗株FZ91-30亲缘性相距较远,表明该分离株为MDPV野毒株;基于VP1-b序列分析IBDV分离株GL1712与中国新型超强毒株HLJ0504同属HLJ0504-like cluster,而基于vVP2序列则与中等偏强毒力参考株同属C2-intermediate IBDV genotype,为基因重排毒株。根据发病日龄、临床症状、病理剖检和实验室诊断,确诊该临床发病番鸭群混合感染了番鸭细小病毒和传染性法氏囊病病毒。
In December 2017, a group of young Muscovy ducks on a farm in Guangxi exhibited slow growth, clinical symptoms of soft feet, diarrhea with white feces and certain neurological symptoms. The necropsy revealed hepatomegaly with hemorrhage, fibrinous exudate in the abdominal cavity, different degrees of hyperemia in the intestinal mucosa, bursal atrophy and other lesions. The disease started in the ducks when they were 20-day-old; and when they were 30-day-old, their morbidity and mortality reached 20% and 10% respectively. Then, their lesion tissues were aseptically sampled for nucleic acid-specific detection and isolation of MDPV and IBDV. The results showed that the samples were all positive and a Muscovy duck parvovirus(named GX1712) and an infectious bursal disease virus(named GL1712) were isolated. Gene sequence analysis showed that the isolate GX1712 had the closest similarity of 98.5%～99.1% to the MDPV reference strains GX5, P1988 and SAASSHNH, and was located in the same branch in the phylogenetic analysis but was far away from the vaccine strain
In December 2017, a group of young Muscovy ducks on a farm in Guangxi exhibited slow growth, clinical symptoms of soft feet, diarrhea with white feces and certain neurological symptoms. The necropsy revealed hepatomegaly with hemorrhage, fibrinous exudate in the abdominal cavity, different degrees of hyperemia in the intestinal mucosa, bursal atrophy and other lesions. The disease started in the ducks when they were 20-day-old; and when they were 30-day-old, their morbidity and mortality reached 20% and 10% respectively. Then, their lesion tissues were aseptically sampled for nucleic acid-specific detection and isolation of MDPV and IBDV. The results showed that the samples were all positive and a Muscovy duck parvovirus(named GX1712) and an infectious bursal disease virus(named GL1712) were isolated. Gene sequence analysis showed that the isolate GX1712 had the closest similarity of 98.5%～99.1% to the MDPV reference strains GX5, P1988 and SAASSHNH, and was located in the same branch in the phylogenetic analysis but was far away from the vaccine strain
引文
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[3] 戴银, 张丹俊, 沈学怀, 等. 安徽番鸭细小病毒的分离和PCR检测[J].中国兽医杂志, 2016,52(8):35-37.
[4] Glávits R, Zolnai A, Szabó E, et al. Comparative pathological studies on domestic geese (Anser anser domestica) and Muscovy ducks (Cairina moschata) experimentally infected with parvovirus strains of goose and Muscovy duck origin[J].Acta Vet Hung, 2005,53(1):73-89.
[5] 周宗安, 王永山, 邓小昭, 等. 传染性法氏囊病病毒的生态学与流行病学研究[J].中国兽医学报, 1998(5):430-433.
[6] 王永山, 周宗安,邓小昭,等.传染性法氏囊病病毒不同分离株vp2基因部分核酸序列分析[J].中国病毒学, 2003,18(1):68-71.
[7] 王永山, 周宗安, 翟春生, 等. 麻雀自然感染鸡传染性法氏囊病病毒的调查[J].中国兽医学报, 1994(3):268-270.
[8] Kasanga C J, Yamaguchi T, Wambura P N, et al. Detection of infectious bursal disease virus (IBDV) genome in free-living pigeon and guinea fowl in Africa suggests involvement of wild birds in the epidemiology of IBDV[J].Viru Gen, 2008,36(3):521-529.
[9] 韦平, 丁家波. 几种引起家禽免疫抑制的病毒性疾病及其作用机理[J].中国预防兽医学报, 2000,22(4):316-318.
[10] Palya V, Zolnai A, Mato T, et al. Short beak and dwarfism syndrome(SBDS)of mule duck caused by a distinct lineage of goose parvoviruses[J].Avian Pathol, 2009, 38(2):175-180.
[11] 韦平, 龙进学, 阳秀英, 等. 传染性法氏囊病病毒(IBDV)快速检测与分型技术[J].中国兽医学报, 2004,24(4):313-316.
[12] He X, Xiong Z, Yang L, et al. Molecular epidemiology studies on partial sequences of both genome segments reveal that reassortant infectious bursal disease viruses were dominantly prevalent in southern China during 2000—2012[J].Arch Virol, 2014,159(12):3279-3292.
[13] 林世棠, 郁晓岚, 陈炳钿, 等. 一种新的雏番鸭病毒性传染病的诊断[J].中国预防兽医学报, 1991(2):25-26.
[14] 张云, 耿宏伟, 郭东春, 等. 鹅和番鸭细小病毒全基因克隆与序列分析[J].中国预防兽医学报, 2008,30(6):415-419.
[15] 黄安国, 蒋玉雯, 盘宝进, 等. 广西番鸭细小病毒的分离和鉴定[J].广西畜牧兽医, 2002,18(6):5-7.
[16] 黄瑜, 万春和, 傅秋玲, 等. 新型番鸭细小病毒的发现及其感染的临床表现[J].福建农业学报, 2015(5):442-445.
[17] 卢家平, 朱丽娴. 雏番鸭细小病毒和鸭疫里氏杆菌混合感染的诊治[J].中国家禽, 2004(21):29-30.
[18] 罗志英. 雏番鸭花肝病与细小病毒混合感染的诊治[J].中国动物检疫, 2013(5):68-69.
[19] 崔国杰, 陈少莺. 番鸭细小病毒和小鹅瘟病毒混合感染的诊治[J].畜牧与兽医, 2015,47(3):147-148.
[20] 鲜思美, 汪德生, 王春文. 番鸭细小病毒、鸭瘟病毒与沙门菌混合感染的诊断[J].中国家禽, 2012,34(22):54-56.
[21] 吕红超. 新型鸭细小病毒病流行病学研究进展[J].畜牧与饲料科学, 2018,39(2):106-108.
[22] Yu K, Ma X, Sheng Z, et al. Identification of Goose-Origin Parvovirus as a Cause of Newly Emerging Beak Atrophy and Dwarfism Syndrome in Ducklings[J].J Clin Microbiol, 2016,54(8):1999-2007.
[2] 殷震. 动物病毒学[M].北京:科学出版社, 1997.
[3] 戴银, 张丹俊, 沈学怀, 等. 安徽番鸭细小病毒的分离和PCR检测[J].中国兽医杂志, 2016,52(8):35-37.
[4] Glávits R, Zolnai A, Szabó E, et al. Comparative pathological studies on domestic geese (Anser anser domestica) and Muscovy ducks (Cairina moschata) experimentally infected with parvovirus strains of goose and Muscovy duck origin[J].Acta Vet Hung, 2005,53(1):73-89.
[5] 周宗安, 王永山, 邓小昭, 等. 传染性法氏囊病病毒的生态学与流行病学研究[J].中国兽医学报, 1998(5):430-433.
[6] 王永山, 周宗安,邓小昭,等.传染性法氏囊病病毒不同分离株vp2基因部分核酸序列分析[J].中国病毒学, 2003,18(1):68-71.
[7] 王永山, 周宗安, 翟春生, 等. 麻雀自然感染鸡传染性法氏囊病病毒的调查[J].中国兽医学报, 1994(3):268-270.
[8] Kasanga C J, Yamaguchi T, Wambura P N, et al. Detection of infectious bursal disease virus (IBDV) genome in free-living pigeon and guinea fowl in Africa suggests involvement of wild birds in the epidemiology of IBDV[J].Viru Gen, 2008,36(3):521-529.
[9] 韦平, 丁家波. 几种引起家禽免疫抑制的病毒性疾病及其作用机理[J].中国预防兽医学报, 2000,22(4):316-318.
[10] Palya V, Zolnai A, Mato T, et al. Short beak and dwarfism syndrome(SBDS)of mule duck caused by a distinct lineage of goose parvoviruses[J].Avian Pathol, 2009, 38(2):175-180.
[11] 韦平, 龙进学, 阳秀英, 等. 传染性法氏囊病病毒(IBDV)快速检测与分型技术[J].中国兽医学报, 2004,24(4):313-316.
[12] He X, Xiong Z, Yang L, et al. Molecular epidemiology studies on partial sequences of both genome segments reveal that reassortant infectious bursal disease viruses were dominantly prevalent in southern China during 2000—2012[J].Arch Virol, 2014,159(12):3279-3292.
[13] 林世棠, 郁晓岚, 陈炳钿, 等. 一种新的雏番鸭病毒性传染病的诊断[J].中国预防兽医学报, 1991(2):25-26.
[14] 张云, 耿宏伟, 郭东春, 等. 鹅和番鸭细小病毒全基因克隆与序列分析[J].中国预防兽医学报, 2008,30(6):415-419.
[15] 黄安国, 蒋玉雯, 盘宝进, 等. 广西番鸭细小病毒的分离和鉴定[J].广西畜牧兽医, 2002,18(6):5-7.
[16] 黄瑜, 万春和, 傅秋玲, 等. 新型番鸭细小病毒的发现及其感染的临床表现[J].福建农业学报, 2015(5):442-445.
[17] 卢家平, 朱丽娴. 雏番鸭细小病毒和鸭疫里氏杆菌混合感染的诊治[J].中国家禽, 2004(21):29-30.
[18] 罗志英. 雏番鸭花肝病与细小病毒混合感染的诊治[J].中国动物检疫, 2013(5):68-69.
[19] 崔国杰, 陈少莺. 番鸭细小病毒和小鹅瘟病毒混合感染的诊治[J].畜牧与兽医, 2015,47(3):147-148.
[20] 鲜思美, 汪德生, 王春文. 番鸭细小病毒、鸭瘟病毒与沙门菌混合感染的诊断[J].中国家禽, 2012,34(22):54-56.
[21] 吕红超. 新型鸭细小病毒病流行病学研究进展[J].畜牧与饲料科学, 2018,39(2):106-108.
[22] Yu K, Ma X, Sheng Z, et al. Identification of Goose-Origin Parvovirus as a Cause of Newly Emerging Beak Atrophy and Dwarfism Syndrome in Ducklings[J].J Clin Microbiol, 2016,54(8):1999-2007.
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