一株耐盐菌的分离鉴定及其孔雀石绿脱色的研究
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摘要
从舟山双峰盐场的盐田中分离筛选到一株对孔雀石绿具有较强脱色能力的细菌,经鉴定为腐生葡萄球菌(Staphylococcus saprophyticus sp. JJ-1)。研究孔雀石绿的浓度、脱色pH、氯化钠浓度及氧气对染料脱色的影响,并对脱色产物进行紫外-可见吸收光谱分析,HPLC分析和GC-MS分析,以揭示孔雀石绿的降解产物。脱色反应条件初步研究表明,Staphylococcus saprophyticus sp. JJ-1在4 h内对200 mg·L-1孔雀石绿脱色率可高达95%;该菌在低浓度孔雀石绿(<50 mg·L-1)时能够在1 h内快速脱色;在有氧和厌氧条件下该菌的脱色率基本保持一致;在15%NaCl,200mg·L-1孔雀石绿条件下,5 h后的脱色率为94%;该菌脱色的适宜p H 7～10,培养时间4 h,脱色率达到95%。孔雀石绿的主要降解产物为4-(二甲氨基)二苯甲酮。
One bacterial strain, with excellent capability of malachite green decolorization, was isolated from salt pans of Zhoushan Shuangfeng saltern. This strain was identified as Staphylococcus saprophyticus sp. JJ-1. The effect of various parameters(concentration of malachite green, pH, concentration of NaCl and oxygen) on the removal of malachite green was evaluated. Decolorization products were analyzed by UV-visible analysis, HPLC analysis and GC-MS analysis. The results showed that the decolorization efficiency was up to 95% after 4 h when malachite green concentration was 200 mg·L-1. The malachite green with low concentration(<50 mg·L-1) was rapidly decolorized within 1 h. There is no significant difference of decolorization efficiency between aerobic and anaerobic conditions. The decolorization efficiency of 200 mg·L-1 malachite green after 5 h was 94% in the presence of 15% NaCl, the decolorization efficiency was high between p H 7-10, which reached 95% after 4 h. The degradation products of malachite green was 4-(dimethylamino) benzophenone.
One bacterial strain, with excellent capability of malachite green decolorization, was isolated from salt pans of Zhoushan Shuangfeng saltern. This strain was identified as Staphylococcus saprophyticus sp. JJ-1. The effect of various parameters(concentration of malachite green, pH, concentration of NaCl and oxygen) on the removal of malachite green was evaluated. Decolorization products were analyzed by UV-visible analysis, HPLC analysis and GC-MS analysis. The results showed that the decolorization efficiency was up to 95% after 4 h when malachite green concentration was 200 mg·L-1. The malachite green with low concentration(<50 mg·L-1) was rapidly decolorized within 1 h. There is no significant difference of decolorization efficiency between aerobic and anaerobic conditions. The decolorization efficiency of 200 mg·L-1 malachite green after 5 h was 94% in the presence of 15% NaCl, the decolorization efficiency was high between p H 7-10, which reached 95% after 4 h. The degradation products of malachite green was 4-(dimethylamino) benzophenone.
引文
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[2]SARATALE R G,SARATALE G D,CHANG J S,et al.Bacterial decolorization and degradation of azo dyes[J].JTaiwan Inst Chem E,2011,42(1):138-157.
[3]TURHAN K,DURUKAN I,OZTURKCAN S A,et al.Decolorization of textile basic dye in aqueous solution by ozone[J].Dyes Pigments,2012,92(3):897-901.
[4]ALI S,KHATRI Z,KHATRI A,et al.Integrated desizing-bleaching-reactive dyeing process for cotton towel using glucose oxidase enzyme[J].J Clean Prod,2014,66(2):562-567.
[5]SHARMA D K,SAINI H S,SINGH M,et al.Isolation and characterization of microorganisms capable of decolorizing various triphenylmethane dyes[J].J Basic Microb,2004,44(1):59-65.
[6]REN S,GUO J,ZENG G,et al.Decolorization of triphenylmethane,azo,and anthraquinone dyes by a newly isolated Aeromonas hydrophila strain.[J].Appl Microbiol Biot,2006,72(6):1316-1321.
[7]SHARMA D K,SAINI H S,SINGH M,et al.Biological treatment of textile dye acid violet-17 by bacterial consortium in an up-flow immobilized cell bioreactor[J].Lett Appl Microbiol,2010,38(5):345-350.
[8]JANG M S,LEE Y M,CHOI Y L,et al.Isolation of Citrobacter sp.mutants defective in decolorization of brilliant green by transposon mutagenesis[J].J Microbiol,2004,44(4):320-324.
[9]PARSHETTI G,KALME S,SARATALE G,et al.Biodegradation of Malachite Green by Kocuria rosea MTCC1532[J].Acta Chimica Slovenica,2006,53(4):492-498.
[10]SANI R K,BANERJEE U C.Decolorization of triphenylmethane dyes and textile and dye-stuff effluent by Kurthia sp.[J].Enzyme Microb Tech,1999,24(7):433-437.
[11]成文,曾宝强.孔雀绿染料的微生物脱色研究[J].应用与环境生物学报,2000,6(4):370-373.
[12]任倩,蒋丽娟,宋炜,等.孔雀石绿降解菌M3的分离鉴定及降解特性研究[J].生态与农村环境学报,2007,23(3):65-69.
[13]林少芳,余萍,林玉满.一株绿脓假单胞菌对碱性孔雀绿脱色的初步研究[J].福建师范大学学报(自然科学版),2004,20(4):72-75.
[14]金显春,陶文沂.菌株XC6对10种染料的生物脱色作用[J].食品与生物技术学报,2007,26(2):67-70.
[15]吴茵,陈敏,刘洁.刺芹侧耳对孔雀石绿的脱色降解及其产物分析[J].环境科学学报,2016,36(8):2844-2851.
[16]PATEL V R,BHATT N S,B?BHATT H.Involvement of ligninolytic enzymes of Myceliophthora vellerea HQ871747 in decolorization and complete mineralization of Reactive Blue220[J].Chem Eng J,2013,233:98-108.
[17]吴永利,王莉,范子睿,等.孔雀石绿高效脱色菌的鉴定及降解特性研究[J].安徽建筑大学学报,2017,25(1):38-43.
[18]DU L N,ZHAO M,LI G,et al.Biodegradation of malachite green by Micrococcus sp.strain BD15:Biodegradation pathway and enzyme analysis[J].Int Biodeter Biodegr,2013,78(2):108-116.
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