1株具有铁氧化功能的苯胺降解菌的降解特性
|
摘要
使用模拟苯胺废水驯化以海绵铁为载体的SBBR反应器,从中分离出1株高效苯胺降解菌ZL-1,该降解菌具有铁氧化功能。经16S rDNA鉴定,菌株ZL-1为1种克雷伯氏杆菌(Klebsiella sp.)。通过平行对比实验,考察了菌株ZL-1适宜的生长条件,研究了菌株ZL-1介入下的生物海绵铁体系对苯胺的降解效果。结果表明,菌株ZL-1适宜的pH为4～9,温度为20～35℃,苯胺初始浓度为1 000 mg/L时,降解速率最快。此外,菌株ZL-1作用下的生物海绵铁体系,较单独海绵铁和单独ZL-1菌液体系对苯胺的降解率叠加之和高出10.1%。介入菌株ZL-1的海绵铁体系较介入非铁细菌PF-1的海绵铁体系,铁溶出量高出41.7%。生物海绵铁体系中生物腐蚀与电化学腐蚀共同促进了海绵铁的溶出,尤其是铁细菌作用下的生物海绵铁体系,腐蚀效果更为突出。该体系中存在大量的Fe~(2+),形成了类Fenton反应,提高了苯胺的降解效果。该研究为经济高效降解苯胺废水提供了新思路。
An efficient iron bacteria which can degrade aniline was isolated from the biological sponge iron system that domesticated through aniline simulated wastewater. The strain was identified as Klebsiella sp. by 16S rDNA. In order to study the suitable growing conditions of strain ZL-1, the degradation effect and mechanism of the sponge iron system with strain ZL-1, the contrast experiments were carried out. Results indicated that strain ZL-1 grew well when the initial concentration of aniline was 1000 mg/L, and the optimal conditions for the biodegradation of aniline by strain ZL-1 were at 20～35 ℃ and pH 4～9. In addition, the biological sponge iron system was 10.1% higher than the sum of the aniline degradation rates of the sponge iron system and the bacteria system. Amount of dissolved iron of the sponge iron system inoculated with strain ZL-1 was 41.7% more than inoculated with non-ferrous bacteria PF-1. Biocorrosion and electrochemical corrosion in the biological sponge iron system promote the dissolution of sponge iron together, especially the biological sponge iron system inoculated with strain ZL-1 is more prominent. The existence of a large amount of iron ions in the biological sponge iron system promotes the Fenton-like effect and improves the degradation of aniline. This study provides a new idea for the economical and efficient degradation of aniline wastewater.
An efficient iron bacteria which can degrade aniline was isolated from the biological sponge iron system that domesticated through aniline simulated wastewater. The strain was identified as Klebsiella sp. by 16S rDNA. In order to study the suitable growing conditions of strain ZL-1, the degradation effect and mechanism of the sponge iron system with strain ZL-1, the contrast experiments were carried out. Results indicated that strain ZL-1 grew well when the initial concentration of aniline was 1000 mg/L, and the optimal conditions for the biodegradation of aniline by strain ZL-1 were at 20～35 ℃ and pH 4～9. In addition, the biological sponge iron system was 10.1% higher than the sum of the aniline degradation rates of the sponge iron system and the bacteria system. Amount of dissolved iron of the sponge iron system inoculated with strain ZL-1 was 41.7% more than inoculated with non-ferrous bacteria PF-1. Biocorrosion and electrochemical corrosion in the biological sponge iron system promote the dissolution of sponge iron together, especially the biological sponge iron system inoculated with strain ZL-1 is more prominent. The existence of a large amount of iron ions in the biological sponge iron system promotes the Fenton-like effect and improves the degradation of aniline. This study provides a new idea for the economical and efficient degradation of aniline wastewater.
引文
[1]吕永梅.我国苯胺生产现状与市场分析[J].中国石油和化工经济分析,2006(10):26-30.Lyu Yongmei.Status and market analysis of aniline production in China[J].Economic Analysis of China's Petroleum and Chemical Industry,2006(10):26-30.
[2]钱易,汤鸿霄,文湘华.水体颗粒物和难降解有机物的性质与控制技术原理(下卷):难降解有机物[M].北京:中国环境科学出版社,2000.
[3]Eletskii A.Sorption properties of carbon nanostructures[J].Physics-USPEKHI,2004,47(11):1119-1154.
[4]陶红,高廷耀,刘军,等.13X沸石吸附处理水中苯胺的性能及应用[J].工业水处理,2002,22(10):22-25.Tao Hong,Gao Tingyao,Liu Jun,et al.Properties and application of 13X zeolite adsorption treatment of aniline in water[J].Industrial Water Treatment,2002,22(10):22-25.
[5]李超.吸附技术在水污染控制中的应用研究[D].北京:中国人民解放军军事医学科学院,2017.Li Chao.Application of Adsorption Technology in Water Pollution Control[D].Beijing:Academy of Military Medical Sciences,Chinese People's Liberation Army,2017.
[6]吴勇民,陈新才,楼洪海,等.印染废水中苯胺类化合物处理的研究进展[J].印染,2014,40(2):51-54.Wu Yongmin,Chen Xincai,Lou Honghai,et al.Research progress in the treatment of aniline compounds in printing and dyeing wastewater[J].Dyeing and Printing,2014,40(2):51-54.
[7]杨春维,桂雯雯,张书豪,等.电辅助Fe2+活化过硫酸盐法降解水中苯胺[J].环境科学与技术,2017,40(4):171-175.
[8]郑文婕,刘国,裴昭君.铋酸钠可见光光催化氧化水中苯胺的研究[J].工业安全与环保,2014,40(3):11-13,57.Zheng Wenjie,Liu Guo,Pei Zhaojun.Study on visible light photocatalytic oxidation of aniline in water by sodium citrate[J].Industrial Safety and Environmental Protection,2014,40(3):11-13,57.
[9]Dai J,Sun M,Culp R A,et al.A laboratory study on biochemical degradation and microbial utilization of organic matter comprising a marine diatom,land grass,and salt marsh plant in estuarine ecosystems[J].Aquatic Ecology,2009,43(4):825-841.
[10]程雅楠,陈鸿汉,刘菲.利用正交法分离鉴定苯胺降解菌及对其降解特性的硏究[J].安徽农业科学,2011,39(15):9002-9005.
[11]Mujahid M.Aniline-induced tryptophan production and identification of indole derivatives from three purple bacteria[J].Current Microbiology,2010,61(4):285-290.
[12]Ghiorse W C.Biology of iron and manganese depositing bacteria[J].Annual Review of Microbiology,1984,38(1):515-550.
[13]张艳,王宏武,马鲁铭.铁细菌在水处理方面的应用及影响其生长的因素[J].四川环境,2006,25(5):61-64.Zhang Yan,Wang Hongwu,Ma Luming.Application of iron bacteria in water treatment and factors affecting its growth[J].Sichuan Environment,2006,25(5):61-64.
[14]Voegelin A,Hug S J.Catalyzed oxidation of arsenic(Ⅲ)by hydrogen peroxide on the surface of ferrihydrite:an in situ ATR-FTIR study[J].Environmental Science and Technology,2003,37(5):972-978.
[15]Wang X M,Waite T D.Iron speciation and iron species transformation in activated sludge membrane bioreactors[J].Water Research,2010,44(11):3511-3521.
[16]冯娟娟,王亚娥,李杰,等.新型富铁多孔复合填料强化生化处理效能研究[J].中国给水排水,2013,29(3):24-28.Feng Juanjuan,Wang Ya'e,Li Jie,et al.Study on enhanced biochemical treatment efficiency of new iron-rich porous composite filler[J].China Water&Wastewater,2013,29(3):24-28.
[17]Hyung Yeel,Kahng,Jerome J Kukor.Characterization of strain HY99,a novel microorganism capable of aerobic and anaerobic degradation of aniline[J].FEMS Microbiology Letters,2000(190):215-221.
[18]曾国驱,任随周,曹渭,等.兼性厌氧苯胺降解菌的分离鉴定及其特性[J].环境科学,2006(8):1618-1622.
[19]Fujii T,Takeo M,Macda Y,et al.Cloning and sequencing of a gene cluster for the meta-cleavage pathway of aniline degradation in Acinectobacter sp.strain YAA[J].J Ferment Bioeng,1998(85):514-517.
[20]Emtiazi G,Satarii M,Mazaherion F.The utilization of aniline,chlorinated aniline,and aniline blue as the only source of nitrogen by fungi in water[J].Water Research,2001(35):1219-1224.
[21]张逸飞,顾挺,王国祥,等.一株苯胺降解菌的分离鉴定及其降解特性[J].环境污染与防治,2008,30(2):12-15.
[22]Schnell S,Schink B.Anaerobic aniline degradation via reductive deamination of 4-aminobenzoyl-Co a in desulfo bacterium aniline[J].Arch Microbiol,1991(153):183-190.
[23]张浩,刘玉香,呼婷婷,等.一株苯胺降解菌的分离及其降解特性[J].环境工程学报,2015,9(12):6154-6160.Zhang Hao,Liu Yuxiang,Hu Tingting,et al.Isolation and degradation characteristics of an aniline-degrading bacterium[J].Techniques and Equipment for Environmental Protection,2015,9(12):6154-6160.
[24]刁刻,吴琼,邱业先.一株苯胺降解菌的筛选及其降解效率研究[J].苏州科技大学学报:自然科学版,2017,34(2):43-48,54.Diao Ke,Wu Qiong,Qiu Yexian.Screening and degradation efficiency of an aniline-degrading bacterium[J].Journal of Suzhou University of Science and Technology:Natural Science Edition,2017,34(2):43-48,54.
[25]Sheludchenko M S,Kolomytseva M P,Travkin V M,et al.Degradation of aniline by Delftia tsuruhatensis 14S in batch and continuous processes[J].Applied Bio-chemistry and Microbiology,2005,41(5):465-468.
[26]Wang Dianzhan,Zheng Guanyu,Wang Shimei,et al.Biodegradation of aniline by Candida tropicalis AN1 isolated from aerobic granular sludge[J].Journal of Environmental Sciences,2011,23(12):2063-2068.
[27]李正,王亚娥,李杰.污泥浓度对生物海绵铁体系中Fe0的腐蚀影响研究[J].环境科学与技术,2014,37(3):7-11.
[28]王亚娥,李杰,翟思媛,等.Fe0对SBBR工艺处理腈纶废水性能影响[J].化工学报,2013,64(8):2996-3002.
[29]Voegelin A,Hug S J.Catalyzed oxidation of arsenic(Ⅲ)by hydrogen peroxide on the surface of ferrihydrite:an in situ ATR-FTIR study[J].Environmental Science and Technology,2003,37:972-978.
[30]龙腾锐,孟雪征,赖震宏.Fe3+对活性污泥系统的影响[J].给水排水,2004(12):15-17.Long Tengrui,Meng Xuezheng,Lai Zhenhong.Effect of Fe3+on activated sludge system[J].Water&Wastewater,2004(12):15-17.
[2]钱易,汤鸿霄,文湘华.水体颗粒物和难降解有机物的性质与控制技术原理(下卷):难降解有机物[M].北京:中国环境科学出版社,2000.
[3]Eletskii A.Sorption properties of carbon nanostructures[J].Physics-USPEKHI,2004,47(11):1119-1154.
[4]陶红,高廷耀,刘军,等.13X沸石吸附处理水中苯胺的性能及应用[J].工业水处理,2002,22(10):22-25.Tao Hong,Gao Tingyao,Liu Jun,et al.Properties and application of 13X zeolite adsorption treatment of aniline in water[J].Industrial Water Treatment,2002,22(10):22-25.
[5]李超.吸附技术在水污染控制中的应用研究[D].北京:中国人民解放军军事医学科学院,2017.Li Chao.Application of Adsorption Technology in Water Pollution Control[D].Beijing:Academy of Military Medical Sciences,Chinese People's Liberation Army,2017.
[6]吴勇民,陈新才,楼洪海,等.印染废水中苯胺类化合物处理的研究进展[J].印染,2014,40(2):51-54.Wu Yongmin,Chen Xincai,Lou Honghai,et al.Research progress in the treatment of aniline compounds in printing and dyeing wastewater[J].Dyeing and Printing,2014,40(2):51-54.
[7]杨春维,桂雯雯,张书豪,等.电辅助Fe2+活化过硫酸盐法降解水中苯胺[J].环境科学与技术,2017,40(4):171-175.
[8]郑文婕,刘国,裴昭君.铋酸钠可见光光催化氧化水中苯胺的研究[J].工业安全与环保,2014,40(3):11-13,57.Zheng Wenjie,Liu Guo,Pei Zhaojun.Study on visible light photocatalytic oxidation of aniline in water by sodium citrate[J].Industrial Safety and Environmental Protection,2014,40(3):11-13,57.
[9]Dai J,Sun M,Culp R A,et al.A laboratory study on biochemical degradation and microbial utilization of organic matter comprising a marine diatom,land grass,and salt marsh plant in estuarine ecosystems[J].Aquatic Ecology,2009,43(4):825-841.
[10]程雅楠,陈鸿汉,刘菲.利用正交法分离鉴定苯胺降解菌及对其降解特性的硏究[J].安徽农业科学,2011,39(15):9002-9005.
[11]Mujahid M.Aniline-induced tryptophan production and identification of indole derivatives from three purple bacteria[J].Current Microbiology,2010,61(4):285-290.
[12]Ghiorse W C.Biology of iron and manganese depositing bacteria[J].Annual Review of Microbiology,1984,38(1):515-550.
[13]张艳,王宏武,马鲁铭.铁细菌在水处理方面的应用及影响其生长的因素[J].四川环境,2006,25(5):61-64.Zhang Yan,Wang Hongwu,Ma Luming.Application of iron bacteria in water treatment and factors affecting its growth[J].Sichuan Environment,2006,25(5):61-64.
[14]Voegelin A,Hug S J.Catalyzed oxidation of arsenic(Ⅲ)by hydrogen peroxide on the surface of ferrihydrite:an in situ ATR-FTIR study[J].Environmental Science and Technology,2003,37(5):972-978.
[15]Wang X M,Waite T D.Iron speciation and iron species transformation in activated sludge membrane bioreactors[J].Water Research,2010,44(11):3511-3521.
[16]冯娟娟,王亚娥,李杰,等.新型富铁多孔复合填料强化生化处理效能研究[J].中国给水排水,2013,29(3):24-28.Feng Juanjuan,Wang Ya'e,Li Jie,et al.Study on enhanced biochemical treatment efficiency of new iron-rich porous composite filler[J].China Water&Wastewater,2013,29(3):24-28.
[17]Hyung Yeel,Kahng,Jerome J Kukor.Characterization of strain HY99,a novel microorganism capable of aerobic and anaerobic degradation of aniline[J].FEMS Microbiology Letters,2000(190):215-221.
[18]曾国驱,任随周,曹渭,等.兼性厌氧苯胺降解菌的分离鉴定及其特性[J].环境科学,2006(8):1618-1622.
[19]Fujii T,Takeo M,Macda Y,et al.Cloning and sequencing of a gene cluster for the meta-cleavage pathway of aniline degradation in Acinectobacter sp.strain YAA[J].J Ferment Bioeng,1998(85):514-517.
[20]Emtiazi G,Satarii M,Mazaherion F.The utilization of aniline,chlorinated aniline,and aniline blue as the only source of nitrogen by fungi in water[J].Water Research,2001(35):1219-1224.
[21]张逸飞,顾挺,王国祥,等.一株苯胺降解菌的分离鉴定及其降解特性[J].环境污染与防治,2008,30(2):12-15.
[22]Schnell S,Schink B.Anaerobic aniline degradation via reductive deamination of 4-aminobenzoyl-Co a in desulfo bacterium aniline[J].Arch Microbiol,1991(153):183-190.
[23]张浩,刘玉香,呼婷婷,等.一株苯胺降解菌的分离及其降解特性[J].环境工程学报,2015,9(12):6154-6160.Zhang Hao,Liu Yuxiang,Hu Tingting,et al.Isolation and degradation characteristics of an aniline-degrading bacterium[J].Techniques and Equipment for Environmental Protection,2015,9(12):6154-6160.
[24]刁刻,吴琼,邱业先.一株苯胺降解菌的筛选及其降解效率研究[J].苏州科技大学学报:自然科学版,2017,34(2):43-48,54.Diao Ke,Wu Qiong,Qiu Yexian.Screening and degradation efficiency of an aniline-degrading bacterium[J].Journal of Suzhou University of Science and Technology:Natural Science Edition,2017,34(2):43-48,54.
[25]Sheludchenko M S,Kolomytseva M P,Travkin V M,et al.Degradation of aniline by Delftia tsuruhatensis 14S in batch and continuous processes[J].Applied Bio-chemistry and Microbiology,2005,41(5):465-468.
[26]Wang Dianzhan,Zheng Guanyu,Wang Shimei,et al.Biodegradation of aniline by Candida tropicalis AN1 isolated from aerobic granular sludge[J].Journal of Environmental Sciences,2011,23(12):2063-2068.
[27]李正,王亚娥,李杰.污泥浓度对生物海绵铁体系中Fe0的腐蚀影响研究[J].环境科学与技术,2014,37(3):7-11.
[28]王亚娥,李杰,翟思媛,等.Fe0对SBBR工艺处理腈纶废水性能影响[J].化工学报,2013,64(8):2996-3002.
[29]Voegelin A,Hug S J.Catalyzed oxidation of arsenic(Ⅲ)by hydrogen peroxide on the surface of ferrihydrite:an in situ ATR-FTIR study[J].Environmental Science and Technology,2003,37:972-978.
[30]龙腾锐,孟雪征,赖震宏.Fe3+对活性污泥系统的影响[J].给水排水,2004(12):15-17.Long Tengrui,Meng Xuezheng,Lai Zhenhong.Effect of Fe3+on activated sludge system[J].Water&Wastewater,2004(12):15-17.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |