灵丹多糖胶囊对CCl_4致小鼠肝纤维化的保护作用
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摘要
目的:探讨灵丹多糖胶囊对CCl_4致小鼠肝纤维化的保护作用。方法:以C57BL/6雄性小鼠为研究对象,构建CCl_4诱导肝纤维化模型,予灵丹多糖胶囊干预。记录实验过程中小鼠体质量治疗变化,检测血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)水平,取肝组织做HE染色,天狼星红染色及α-平滑肌肌动蛋白(α-SMA)免疫荧光染色。结果:与模型组比较,灵丹多糖胶囊各浓度组小鼠血清ALT、AST水平均显著下降(P<0.05,P<0.01),肝脏损伤程度减轻,胶原纤维沉积及α-SMA表达水平均显著下降(P<0.05)。结论:灵丹多糖胶囊能降低肝纤维化小鼠血清ALT、AST水平,改善肝损伤,减少α-SMA的沉淀,对小鼠化学性肝纤维化有保护作用。
Objective: To investigate the protective effect of Lingdan Polysaccharide Capsule against carbon tetrachloride(CCl_4)-induce liver fibrosis injury in mice. Methods: C57 BL/6 male mice were used in this study to duplicate CCl_4-induce liver fibrosis model, intervened with Lingdan Polysaccharide Capsule. The weight of mice and the sterum levels of alanine transaminase(ALT) and aspartate aminotransferase(AST) were examed. The liver paraffin sections were made and tested by the Hematoxylin-eosin(HE) and Sirius Red staining. Furthermore, the deposit of α-smooth muscle actin(α-SMA) in liver tissues was analyzed by immunofluorescent. Results: Compared with the model group, the levels of serum ALT, AST of mice in Lingdan Polysaccharide Capsule group were decreased significantly(P<0.05, P<0.01). The degree of liver injury was alleviated, collagen deposition and the expression of α-SMA were significantly decreased(P<0.05). Conclusion: Lingdan Polysaccharide Capsule could notably lower the mice serum ALT and AST elevation caused by the CCl_4, improve liver tissue injury, and inhibit the generation of α-SMA.
Objective: To investigate the protective effect of Lingdan Polysaccharide Capsule against carbon tetrachloride(CCl_4)-induce liver fibrosis injury in mice. Methods: C57 BL/6 male mice were used in this study to duplicate CCl_4-induce liver fibrosis model, intervened with Lingdan Polysaccharide Capsule. The weight of mice and the sterum levels of alanine transaminase(ALT) and aspartate aminotransferase(AST) were examed. The liver paraffin sections were made and tested by the Hematoxylin-eosin(HE) and Sirius Red staining. Furthermore, the deposit of α-smooth muscle actin(α-SMA) in liver tissues was analyzed by immunofluorescent. Results: Compared with the model group, the levels of serum ALT, AST of mice in Lingdan Polysaccharide Capsule group were decreased significantly(P<0.05, P<0.01). The degree of liver injury was alleviated, collagen deposition and the expression of α-SMA were significantly decreased(P<0.05). Conclusion: Lingdan Polysaccharide Capsule could notably lower the mice serum ALT and AST elevation caused by the CCl_4, improve liver tissue injury, and inhibit the generation of α-SMA.
引文
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[12]张艳,李卉,满成云,等.基于气相色谱-质谱联用技术的平菇多糖治疗大鼠急性肝损伤的代谢组学研究.食品工业科技,2018,39(20):303-307
[13]邱赛红,黄湘珺,黄宇明,等.灵芝及其提取物减轻肝损伤作用的研究概况.湖南中医药大学学报,2017,37(4):461-464
[14]吴丽娟,赵峡,王伟,等.几种海洋类肝素多糖的制备及抗乙肝病毒活性初步研究.中国海洋药物,2016,35(6):32-37
[15]李天一,汪丽佩,吴国琳.黄芪多糖对免疫性肝损伤小鼠免疫调节的影响.中国中医急症,2014,23(1):25-27
[16]高雅,张可兰,钟明利,等.狗肝菜多糖通过抗炎抗氧化抑制酒精高糖高脂引起的大鼠肝损伤.中国药理学通报,2016,32(12):1665-1669
[2]宋雨鸿,刘强,徐舒,等.丹参多糖对小鼠免疫性肝损伤的保护作用.中南药学,2013,11(5):345-348
[3]姚莹华,刘强,陈育尧,等.丹参多糖对小鼠急性肝损伤的保护作用.中国实验方剂学杂志,2010,16(6):227-230
[4]Song Y H,Liu Q,Lv Z P,et al.Protection of a polysaccharide fromSalvia miltiorrhiza,a Chinese medicinal herb,against immunologicalliver injury in mice.Int J Biol Macromol,2008,43(2):170-175
[5]Sun X G,Fu X Q,Cai H B,et al.Proteomic analysis of protectiveeffects of polysaccharides from Salvia miltiorrhiza againstimmunological liver injury in mice.Phytother Res,2011,25(7):1087-1094
[6]陈玉胜,陈全战.灵芝多糖对CCl4诱导的急性肝损伤小鼠的抗炎和保肝活性.食品科学,2017,38(17):210-215
[7]黄进,张晨,詹菲,等.黄芪多糖对肝纤维化大鼠TGF-β1/Smads信号通路的影响.中华中医药杂志,2015,30(6):2184-2186
[8]Zhong W,Gao L,Zhou Z,et al.Indoleamine 2,3-dioxygenase 1deficiency attenuates CCl4-induced fibrosis through Th17 cellsdown-regulation and tryptophan 2,3-dioxygenase compensation.Oncotarget,2017,8(25):40486-40500
[9]夏海珊,陈少茹,钟月春,等.肝纤维化的发病机制和药物治疗现况.中国医药导报,2014,11(18):162-165
[10]黄宇,邓鑫,梁健.中西医结合药物治疗乙肝肝纤维化的研究进展.辽宁中医杂志,2018,45(4):878-882
[11]穆娟,赵明峰,李玉明.丹参在药理作用的研究现况.当代医学,2017,23(27):182-184
[12]张艳,李卉,满成云,等.基于气相色谱-质谱联用技术的平菇多糖治疗大鼠急性肝损伤的代谢组学研究.食品工业科技,2018,39(20):303-307
[13]邱赛红,黄湘珺,黄宇明,等.灵芝及其提取物减轻肝损伤作用的研究概况.湖南中医药大学学报,2017,37(4):461-464
[14]吴丽娟,赵峡,王伟,等.几种海洋类肝素多糖的制备及抗乙肝病毒活性初步研究.中国海洋药物,2016,35(6):32-37
[15]李天一,汪丽佩,吴国琳.黄芪多糖对免疫性肝损伤小鼠免疫调节的影响.中国中医急症,2014,23(1):25-27
[16]高雅,张可兰,钟明利,等.狗肝菜多糖通过抗炎抗氧化抑制酒精高糖高脂引起的大鼠肝损伤.中国药理学通报,2016,32(12):1665-1669
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